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단기간 침수처리 하에서 유채의 생리적 반응, 지방산 조성과 수확량
구양규,박원,방진기,장영석,김용범,배현종,서미정,안성주 (사) 한국생물환경조절학회 2009 시설원예‧식물공장 Vol.18 No.2
The effects of short-term waterlogging on physiological responses, fatty acid compositions and yield components of rapeseed at both the vegetative growth and the reproductive growth stages were assessed in this study. Waterlogged plants were treated for a period of 10 days at the vegetative growth stage and 4 days at the reproductive growth stage. The results show that photosynthesis and stomatal conductance at both the vegetative growth and the reproductive growth stage were significantly reduced during the waterlogging period and after the recovery period. When the plants were removed from water, photosynthesis and stomatal conductance progressively restored similar values to those of control plants within 2~3 days. Fatty acid compositions were unaffected by waterlogging treatment. However, yield components (pod number and pod length) of the waterlogged treated plants at the reproductive growth stage were significantly reduced. These results suggest that short-term waterlogging may thus influence oilseed yield component.
구양규,D.J. 울리,안성주 한국원예학회 2008 Horticulture, Environment, and Biotechnology Vol.49 No.4
This study explored the influence of N-(2-chloro-4-pyridyl)-N-phenylurea (CPPU) treatment of the roots on shoot growth and root growth, and investigated the effects of a GA3 foliar spray on reversing the growth suppression of chilli ‘Ancho St Luis’ shoots due to CPPU treatment of the root system. In the first experiment, six-week-old chilli seedling roots were soaked in an aqueous solution of CPPU (10 mg・L-1) for two hours, transplanted into pots and grown in a glasshouse. In the second experiment, the roots of six-week-old seedlings were soaked in three concentrations (1, 5, or 10 mg・L-1) of CPPU for two hours, transplanted into pots, and grown in a glasshouse. Four weeks after CPPU treatment, the plants were sprayed with gibberellic acid (GA3) at 100 mg・L-1 as a single foliar spray. Results showed that root applied CPPU at 10 mg・L-1 significantly decreased plant height, probably by inhibiting the activity of the sub-apical region of shoot, reducing internode number and length at 2 and 4 weeks after treatment, and suppressing root growth. CPPU-treated plants also resulted in delayed flowering. Application of GA3 to shoots did not reverse CPPU effect. Therefore, CPPU applied to roots may not only block GA3 biosynthesis but also block GA3 action.
유채의 $F_1$ 종자 생산시 종자친과 화분친의 재식비 및 등숙시기별 종자의 지방산 분석
구양규,양선영,정용수,김현성,서미정,안성주,Ku, Yang-Gyu,Yang, Sun-Young,Jung, Yong-Su,Kim, Hyun-Sung,Suh, Mi-Chung,Ahn, Sung-Ju 한국작물학회 2010 한국작물학회지 Vol.55 No.3
이 실험의 목적은 유채 $F_1$ 종자 생산시 종자친과 화분친의 재식비 및 수확시기별 종자의 지방산 함량에 영향을 미치는지 알아보고자 실험을 수행하였다. 안정적인 종자 생산을 위해, 두 가지의 실험을 노지에서 수행하였다. 실험 1은 $F_1$ 종자 생산을 위한 종자친과 화분친의 재식 비별(4:2, 10:2, 10:1)로 정식하였고 유채 개화 후 40, 45, 50, 55, 60에 지방산 함량을 조사하였다. 실험 2는 $F_2$ 종자의 개화 후 등숙시기별로 종자를 수확하여 지방산을 분석하였다. 실험의 결과, 종자친과 화분친 재식비에 따른 등숙시기별 유채 종자의 지방산 조성 및 함량은 차이를 보여 주었으나 지방산의 오염 정도 척도인 erucic acid는 재식비에 따라 영향을 미치지 않았다. 종자친과 화분친의 비에서 얻어진 $F_1$과 $F_2$ 종자 비에서 palmitic acid, stearic acid과 linoleic acid은 등숙 시기에 따라 감소하였다. 그러나 oleic acid는 두 실험 모두에서 개화 후 55일까지 상대적으로 증가하였지만, 개화후 60일째 영향을 미치지 않았다. 지방산 생합성 관련 유전자인 SAD, FAD1 및 FAD2의 발현양상도 등숙 시기별 지방산 조성 및 함량과 일치하였다. 이런 결과는 종자친과 화분친의 비에 따른 재식비과 $F_2$종자의 비에 따른 지방산 조성이 큰 문제가 되지 않음을 보여주므로 $F_1$ 종자의 안정적인 생산을 위해 종자친의 파종비는 늘리고 화분친비를 줄이면 유채 종자 생산량이 높아질 것으로 사료가 된다. The objective of this experiment was determined to investigate the effect of the planting ratio of Male Sterility (MS) to Restorer Line (RL) and harvesting time on fatty acid compositions under $F_1$ seed production of Brassica napus L. For rapeseed seed production, two experiments were conducted in the open fields. One experiment studied planting ratios of MS to RL (4:2, 10:2, or 10:1) were planted and investigated fatty acid composition at 40, 45, 50, 55, and 60 days after flowering, the other $F_2$ seeds were analyzed on fatty acid compositions of harvested seeds at five sequential stages. The results showed that fatty acid compositions of developing seeds were influenced by MS:RL planting ratios and $F_2$ hybrid treatments and contaminated level of fatty acid compositions, erucic acid, were unaffected by planting ratio of MS to RL. Fatty acid compositions such as palmitic acid (C16:0), stearic acid (C18:0) and linoleic acid (C18:2) contents decreased during seed maturation period in $1^{st}$ and $2^{nd}$ experiments. In contrast, oleic acid (C18:1) content relatively increased up to 55days after flowering. At day 60 after flowering, oleic acid content was unaffected by MS:RL planting ratios and $F_2$ seeds treatments. Aspects of related gene expression of fatty acid synthesis such as SAD, FAD1 and FAD2 were followed exactly to changes of fatty acid composition during seed maturation. These results suggest that MS ratio may be enlarged and RL may be reduced, indicating this ratio will be useful for rape seed production.
구양규,D. J. 울리,안성주 한국원예학회 2008 Horticulture, Environment, and Biotechnology Vol.49 No.5
This experiment was designed to further evaluate the effect of root applied N-(2-chloro-4-pyridyl)-N -phenylurea (CPPU) on plant growth, chlorophyll content, leaf water potential and photosynthetic rate of capsicum plants. The roots of 6-week-old capsicum seedlings were soaked in 2 concentrations (1 or 10 mg・L-1) of CPPU for 2 hours. Root applied CPPU at 10 mg・L-1 treated plants significantly decreased plant height and leaf expansion, and by 3 weeks after CPPU treatment, chlorophyll content of 10 mg・L-1 CPPU was significantly lower than untreated controls. Photosynthetic rates were not affected by CPPU treatment. Leaf water potential of the 10 mg・L-1 CPPU-treated plants was lower than untreated controls, possibly due to a small osmotic adjustment as stomatal resistance and photosynthetic rate were not affected. Xylem sap collected from the roots of CPPU-treated capsicum plants had less detectable gibberellin, possibly associated with blocking of gibberellin biosynthesis.
양파 채종과 채종 후 재생구의 변화에 미치는 고온과 고습의 영향
구양규(Yang-Gyu Ku),박원(Won Park),이을태(Eul-Tai Lee),김철우(Cheol-Woo Kim),김유선(Yu-Seon Kim),장영석(Young-Seok Jang),안성주(Sung-Ju Ahn) 한국원예학회 2008 원예과학기술지 Vol.26 No.2
고온, 고습, 고온+고습 조건하에서 양파 채종량과 모구 수확량에 미치는 영향을 조사하고자 조생종 ‘신선황’, 중생종‘맵시황’, 만생종 ‘천주대고’등을 이용하여 실험을 수행하였다. 모든 양파 품종들의 개화 후 고온, 고습, 고온+고습처리 후32일째 양파를 수확하여 구고, 구중, 구경, 구수와 양파 채종량을 측정하였다. 구경, 구중, 구수 등에는 처리간의 현저한 차이를 보이지 않았지만 고습과 고온+고습 처리구에서는 구고는 현저하게 증가했다. 만생종 ‘천주대고’는 조생종과 중생종에 비해 구고, 구경, 구중의 감소와 더불어 모구의 수가 많아 양파 모구 생산에 감소했다. 대조구와 비교하여 고온, 고습과 고온+고습 처리구에서는 조생종, 중생종, 만생종 양파의 채종량이 현저하게 감소했다. 특히 만생종 ‘천주대고’는 다른 품종에 비교해서 고온, 고온+고습 처리에서 피해가 더 심한 것으로 생각된다. This study was undertaken to determine the effect of high temperature, high humidity and high temperature plus high humidity on seed production and mother bulb harvesting of three onion (Allium cepa L.) cultivars of early cultivar ‘Shinsunhwang’, intermediate cultivar ‘Maebsihwang’ and late cultivar ‘Chunjudaego’. At 32 days after treatment, onion plants were harvested and measured mother bulb parameters such as bulb height, diameter, weight and number, and seed yield. The results show that bulb height of the high humidity and high temperature plus high humidity treated plants significantly affected, but bulb diameter, weight and number were unaffected. Significantly decreased bulb yield in the late cultivar ‘Chunjudaego’ would be caused by the reduced bulb height, diameter, weight and increased bulb number. Compared to control, high temperature, high humidity and high temperature plus high humidity treatments significantly decreased seed yield of three onion cultivars such as early, intermediate and late. Especially, onion seed yield of the late cultivar ‘Chunjudaego’ under high temperature and high temperature plus high humidity treatments were considered to be more damaged compared to other cultivars.