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      • SCOPUSKCI등재

        3개의 배양액내에서 생쥐배아의 발달과 Pronase로 처리한 생쥐배아 부화율의 비교 연구

        이정헌,고희정,채규정,이기숙,김종덕,Lee, Jeong-Heon,Go, Hee-Jeong,Chae, Geu-Jeong,Lee, Ki-Suk,Kim, Jong-Duk 대한생식의학회 2001 Clinical and Experimental Reproductive Medicine Vol.28 No.3

        Objectives: The purpose of this present study was to compare mouse embryo development in 3 commercial media and hatching competence of mouse embryo with or without enzymatic treatment. Methods: Collected 375 mouse embryos were divided into three groups, and then cultured in IVF-20 (G2), Medicult IVF (M3), P-1 (blastocyst M), respectively. Three day mouse morulae were cultured in G2 media treated with pronase. The results were analyzed using Chi-square test, and considered statistically significant when p<0.01. Results: The developmental rate of 2 cell mouse embryo after 72 hours was highest in IVF-20 (G2) among conventional 3 media. The hatching rate of mouse morulae was low when clultured in G2 media without pronase during 48 hours. However, it was higher when cultured in media treated with $1{\mu}g/ml$, $2.5{\mu}g/ml$, $5{\mu}g/ml$ pronase, respectively. Conclusions: Using good media and digestion of zona pellucida with enzymatic treatment improve development and hatching rate of embryo. Therefore, implantation and pregnancy rate could be improved.

      • 응급 자궁경부봉축술의 임상적 고찰

        양윤정 ( Yun Jeong Yang ),안은준 ( Eun Jun Ahn ),고희정 ( Hee Jeong Go ),정영주 ( Young Ju Jeong ),조성남 ( Sung Nam Cho ),김종덕 ( Jong Duk Kim ) 전북대학교 의과학연구소 2004 全北醫大論文集 Vol.28 No.1

        이 임상분석의 목적은 자궁경부무력증을 지닌 환자에 있어 응급 자궁경부봉축술이 임신의 연장과 주산기 유병율에 미치는 영향을 밝히는데 있다. 응급 자궁경부봉축술 후 평균임신 유지기간은 76.9±49.6일 이었다. 분만 시 평균 임신 주 수는 31.0±7.2이었으며 평균 출생체중은 1,737±965gm이었다. 23명의 임신부 중 19명이 신생아를 분만하였고, 이중 3명의 신생아가 신생아기에 사망하였다. 주산기 생 존률은 69.9%였고 신생아 생존률운 84.2%였다. 총괄하여 응급 자궁경부봉축술의 성공률은 69.9%(16/23)이었다. 이번 연구를 통하여, 양막의 돌출과 진행된 자궁경부 개대가 동반된 임신부들에게 있어 응급 자궁경부봉축술은 임신기간의 연장과 더불어 주산기 예후를 증진시킬 수 있었다. Objective: The aim of this clinical evaluation was to identify the effect of emergency cervical cerclage on prolonging pregnancy and perinatal morbidity in patient with cervical incompetence. Methods: From April 1994 to February 2003, twenty-three patients receiving emergency cervical cerclage at our department were retrospectively analyzed. At the time of emergency cerclage, the mean gestational age of the patients was 20.2±3.4 weeks. These patients were compared with thirty patients underwent elective cervical cercalge in the same period and place. Results: The mean maintaining period of pregnancy after emergency cerclage was 76.9±49.6 days. The mean gestational age at delivery was 31.0±7.2 weeks and the mean birth weight was 1,737±965 gm. Nineteen of twenty-three pregnant women delivered viable infant but three infants died during the neonatal period. Perinatal survival rate was 69.9%, and neonatal survival rate was 84.2%. Overall the success rate of emergency cerclage was 69.9%(16/23). Conclusion: From this study, it suggested that emergency cerclage in patient with presence of bulging membrane and advanced cervical dilation can achieve prolonging pregnancy and improving perinatal outcome.

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