대두 - 근류균(根瘤菌) 공생(共生)에서 뿌리 분비물(分泌物)과 화학주성(化學走性)이 근류형성에 미치는 영향

강상재,박우철 한국농화학회 1993 Applied Biological Chemistry (Appl Biol Chem) Vol.36 No.3

인공고환 성형술에 대한 고찰

강상재,한창섭,최성협 인제대학교 1988 仁濟醫學 Vol.9 No.2

남성에 있어서 음낭에 고환이 없다는 것은 일상생활에 지장을 주지 않더라도 신체적 결함에 의한 열등감 때문에 받는 정신적 상처는 심각할 수 있으며 이 경우 인공고환 성형술의 의미는 매우 크다. 저자들은 1984년 7월부터 1987년 10월까지 9명의 환자에서 시술한 10개의 인공고환 성형술을 추적 관찰한 결과 음낭농양과 인공고환 크기 부적합 등의 합병증이 각각 1례씩이었으나 대부분 만족한 결과를 얻었다. The absence of testicle may impart a feeling of inferiority in the male, and the result may be failure of sexual performance. The psychologic problem also may have detrimental effects on a patient's occupation and social life. All patients, unfortunate enough to have an absent testicle should be offered the option of a testicular prosthesis. We report 9 patients with cryptorchidism, testicular torsion and prostate cancer treated with orchiectomy and testicular prosthesis during the past 3 years. The prostheses currently available all result in a satisfactory cosmetic and psychologic result, and surgical complications were very low.

The Effects of Phosphate Starvation on the Activities of Acid and Alkaline Phosphatase, Fructose-1,6-bisphosphatase, Sucrose-phosphate Synthase and Nitrate Reductase in Melon (Cucumis melo L.) Seedlings

강상재,이창희,박만 한국토양비료학회 2016 한국토양비료학회지 Vol.50 No.4

Plants response to phosphate starvation include the changes of activity of some enzymes, such as phosphatases, fructose-1,6-bisphosphatase, sucrose-phosphate synthase and nitrate reductase. In this study, to determine the effects of phosphate starvation on the change of activities of acid and alkaline phosphatase, fructose-1,6-bisphosphatase, sucrose-phosphate synthase, and nitrate reductase were studied in melon seedlings (Cucumis melo L.). The content of the protein and chlorophyll tended to relatively reduced in melon seedlings subjected to phosphate starvation. Acid phosphatase activity in first and second leaves of melon seedlings was relatively higher than that of third and fourth leaves of seedlings in 14 days after phosphate starvation treatment, respectively. Active native-PAGE band patterns of acid phosphatase in melon leaves showed similar to activities of acid phosphatase, whereas alkaline phosphatase activity was different from the change in the activity of acid phosphatase. Inorganic phosphate content in melon seedlings leaves was constant. The changes of Fructose-1,6-bisphosphatase and sucrose phosphate synthase activities showed similar patterns in melon seedlings leaves, and between these enzymes activities and phosphate nutrition negatively related. Fructose-1,6- bisphosphatase and sucrose phosphate synthase activities showed significant difference in second and fourth leaves, but nitrate reductase showed significant difference in first and second leaves in 14days after phosphate starvation treatment. We concluded that phosphate nutrition could affect the distribution of phosphate, carbon and nitrogen in melon seedlings.

Flavonoids와 근류균의 상호작용

강상재,박우철,서상현,Kang, Sang-Jae,Park, Woo-Churl,Seo, Sang-Hyun 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.44 No.4

본 연구는 숙주특이적인 근류형성에 두과작물의 뿌리분비물에 포함된 기주특이적인 flavonoids와 근류균의 상호작용에 대해 실험하였다. Flavonoid 화합물에 대한 근류균의 생물학적 활성은 B. japonocum의 경우 대두의 뿌리분비물에 포함된 flavonoid화합물인 daidzein과 genistein에서는 생장 증진 효과가 나타났으며, alfalfa의 뿌리분비물에 포함된 flavonoid인 luteolin에서는 생장 저해 효과가 나타났으며, R. meliloti의 경우 luteolin의 경우 생장증진 효과가 나타났으며, daidzein과 genistein의 경우 생장저해효과가 나타났다. 각 근류균의 흡수 특이성은 B. japonicum에서 daidzein과 genistein이 각각 $14.95\;{\mu}g/g$와 $14.20\;{\mu}g/g$로 가장 높고 luteolin이 가장 낮게 나타났으며, R. meliloti의 경우 luteolin이 $18.31\;{\mu}g/g$으로 가장 높고 daidzein과 genistein이 가장 낮게 나타났다. 이와 같은 사실로 숙주특이적인 flavonoids가 근류균의 숙주특이성에 깊은 관련이 있을 것으로 사료된다. This experiment was carried out to elucidate the biological activity and absorption characteristics of flavonoids in Rhizobium and Bradyrhizobium and to obtain basic information on host specific nodulation by flavonoids in rhizobium-legume symbiosis. The purpose of the present study was to explore the biological activity and the flavonoid absorption indicates that host-specificity is induced by flavonoids in symbiotic nitrogen fixation. Biological activity increased by daidzein and genistein treatment on B. japonicum KCTC 1539 whereas decreased by luteolin treatment but increased by luteolin treatment on R. meliloti whereas decreased by daidzein and genistein treatment. Daidzein and genistein are absorbed by B. japonicum, KCTC 1539 at higher rate than other flavonoids. Especially, luteolin was absorbed at a least rate. Luteolin are absorbed by R. meliloti KCTC 2353 at higher rate than other flavonoids. Especially, daidzein and genistein was absorbed at a least rate.

발기부전 환자의 감별 진단에 대한 Rigiscan^ⓡ 검사의 의의

강상재,최성협 인제대학교 1990 仁濟醫學 Vol.11 No.2

성기능장애를 진단하는데 있어서 종래의 진단법들과는 달리 야간 음경발기와 강직도를 동시에 측정할 수 있는 RigiscanR을 이용하여 임상적 응용의 효율성을 살펴 보았다. We studied 30 male patients complaining of impotence. Underlying causes were psycogenic (12 paients) and injuries (18 patients). Rigiscan is preferred than other diagnostic tools of impotence to differentiate psycogenic from organic impotence due to continuous and simultaneous recording of nocturnal penile rigidity and tumescence. The results obtained were as follows : 1.In 21 patients shown normal rigiscan, the number of erection was 1.86±0.91 times per night, duration was 12.39±6.41 minutes at tip and 17.03±8.95 minutes at base, tumescence change was 2.86±0.73cm at tip and 2.90±0.68cm at base, rigidity was 66.90±17.08% at tip and 87.85±13.31% at base. 2.In 9 patients shown abnormal rigiscan, the number of erection was 0 per night, duration was 0.92±1.64 minutes at tip and 2.43±3.59 minutes at base. tumescence change was 1.46±0.78cm at tip and 1.69±0.79cm at base, rigidity was 8.26±15.64% at tip and 24.68±30.21% at base. 3.Normal rigiscan showed in 21 patients (11 of psychogenic, 10 of injured patient) but quality of normal event, duration and rigidity were poor.

大豆組織培養細胞 : Rhizobium에 依한 窒素固定力 Rhizobium Association through Tissue Culture Technique in Soybean

姜相載,朴愚喆 慶北大農學誌編輯委員 1986 慶北大農學誌 Vol.4 No.-

Rhizobium의 Nitrogenase 生成要因과 感染機作을 究明하고 培養細胞와 Rhizobia의 混合培養에서 窒養固定係를 確立하기 爲하여 黃金, 南川, D68-0099等 세 品種을 組織培養한 結果는 다음과 같다. Callus 形成能은 胚와 幼根에서는 良好하나 胚軸에서는 전혀 없었으며 2㎎/ℓ 2, 4-D, 4㎎/ℓ NAA에서 가장 良好하고 2, 4-D/Kinetin 組合濃度에서는 0.2㎎(2,4-D)/ℓ 과 0.05㎎(Kinetin)/ℓ에서 가장 理想的이었다. 培養細胞의 成長에는 2,4-D 2㎎/ℓ와 2,4-D(0.2㎎/ℓ)/Kinetin(0.05㎎/ℓ)일 때가 가장 良好하며 R. japonicum 019, 011을 接種하였을때 培養細胞의 成長은 상당히 둔화되었다. 單一 아미노산은 培養細胞의 成長을 沮害 하였는데 黃金의 경우 Methionine, Leucine에서 沮害가 가장 컷으며 다른 아미노산의 添加로 沮害作用이 상당히 회복되었다. 不定根의 生成은 2, 4 -D 2.0㎎/ℓ 에서나 0.2㎎/ℓ 2, 4-D/0.05㎎/ℓ Kinetin에서 良好하였다. 培養細胞-Rhizobium의 親和에 依한 窒養固定力은 25個 使用 菌株中에서 黃金에서는 10個 菌株, 南川에서는 7個 菌株에서 나타났으며 D68-0099에서는 전혀 나타나지 않았으며 黃金의 境遇 85-HG-1 019, 007, 南川에서는 007, 119等이 높은 活性을 나타내었다. This experiment was carried out to elucidate the factor of nitrogenase formation and to establish the nitrogen fixation system in mixed culture of cultured cells and rhizobia through tissue culture technique using three soybean varieties, Hwangkeum, Namcheon and D 68-0099 as host plants. The results obtained were as follows; The callus was induced in embryo and radicle, but not in hypocotyl. The most favorable callus induction was caused by the individual application of 2, 4-D and NAA at the concentration of 2㎎/1 and 4㎎/1, respectively, but in case of treating both 2, 4-D and kinetin, that was done at the concentration of 0.2㎎(2, 4-D)/0.05㎎(kinetin)per liter. The growth of cultured cell was good at the concentration of 2.0㎎(2, 4-D)/1 and 0.2㎎(2, 4-D)/0.05㎎(kinetin)per liter. When cultured cells were inoculated with R. japonicum 019 and 011, their growthes were considerably inhibited. The addition of single amino acid inhibited the growth of cultured cells. Hwangkeum was inhibited considerably by methionine and leucine. The inhibition of growth by single amino acid can be abolished by the addition of certain amino acids. The differentiation of adventitious root was good at the concentration of 2.0㎎ 2, 4-D and 0.2㎎ 2, 4-D/0.05㎎ kinetin per liter. Of three host plants tested with 25 R. japonicum strains, Hwangkeum had affinity for 10 strains, Namcheon for 7 strains and D68-0099 for none. The nitrogen fixing abilities of Hwangkeum and Namcheon caused by cultured cell-Rhizobium association were high in strain 019, 007, and in 007 mixed with 119, respectively.

하대정맥후요관 1례

강상재,한창섭,최성협,김종덕 인제대학교 1988 仁濟醫學 Vol.9 No.2

The retrocaval ureter is a rare congenital venous anomaly in which ureter passes behind the inferior vena cava, leading to varying degrees of ureteral compression. This condition was first described by Hochstetter in 1893. We report a 54 years old male patient who was admitted to our hospital due to intermittent right flank dull pain and managed with the diagnosis of retrocaval ureter. An IVP demonstrated delayed excretion on the right side with pyelocaliectasis and ureterectasis of the upper third of the ureter on subsequent films. A combined retrograde pyelogram and venacavogram delineated the retrocaval ureter. After the ureter was excised and relocated anterior to the vena lava, spatulated end to end anastomosis was made.

음낭내 및 후복강의 Cystic Lymphangioma 1례

강상재,한창섭,이성영,최성협 大韓泌尿器科學會 1988 Korean Journal of Urology Vol.29 No.5

원발성 폐색증 거대요관 6례

강상재,조의제,한창섭,최성협 大韓泌尿器科學會 1989 Korean Journal of Urology Vol.30 No.2

충격파에 의한 요로결석분쇄의 실험적 관찰 (II)

강상재,최성협 大韓泌尿器科學會 1990 Korean Journal of Urology Vol.31 No.1