자돈(雌豚)의 난관간막(卵管間膜), 난소간막(卵巢間膜) 및 난관채(卵管綵)에 형성(形成)된 낭종(囊腫)에 관한 연구(硏究)

강병규,손성완,Kang, Byong-kyu,Son, Seong-wan 대한수의학회 1984 大韓獸醫學會誌 Vol.24 No.1

Gross and histological findings were obtained on cysts formed in the reproductive organs, particularly in the mesosalpinx, mesovarium, and fimbria, of 144 gilts and 37 sows. Of the 181 gilts and sows, 63 (34.8%) were found to have cysts which totaled 89. The number of cysts totaled 38 for the gilts and 51 for the sows, with relative frequencies of 18.1% and 48.6%, respectively. The total number of cysts and the incidence were greater in the sows than in the gilts. With regard to the location of cysts, the highest incidence was found in the mesosalpinx (59.6%). The incidence of cysts in the mesovarium and fimbria was relatively low, with 16.9% and 23.6%, respectively. Most cysts were spherical or oval in shape, and below 5.9mm in diameter. A pedunculated cyst was also seen. The bilateral, incidence of the cysts was lower than unilateral, although the incidence was approxmately the same between the gilts and the sows. With regard to the sexual cycle, the high incidence of cysts was found in the follicular phase than in the luteal phase. The cysts putting pressure upon the oviduct were classified into 3 types according to the shape of epithelium and lamina propria. In type I the epithelium had ciliated cells and the lamina propria formed folds. I type 2 ciliated cells existed, but no folds were formed. In type 3, neither ciliated cells nor folds were seen, the histological change, including partial atrophy and lack of folds in the tubal mucosa were seen.

한우(韓牛)의 번식효율(繁殖效率) 증진(增進)에 관한 연구(硏究) -발정주기(發情周期) 및 임신초기(姙娠初期)의 progesterone 농도변화(濃度變化)-

강병규,최한선,이정길,손창호,서국현,Kang, Byong-kyu,Choi, Han-sun,Lee, Chung-gill,Son, Chang-ho,Suh, Guk-hyun 대한수의학회 1990 大韓獸醫學會誌 Vol.30 No.2

A study was conducted to improve the reproductive performance of Korean native cows. Plasma progesterone concentrations were determined by radioimmunoassay during the estrous cycle and early pregnancy. Plasma progesterone concentrations during the estrous cycle in the 15 cows were the lowest (0.30ng/ml) at estrus, gradually increased after 6 days, remained high from 8 to 17 days (3.87~5.59ng/ml) and drastically decreased thereafter to reach minimal concentrations at the next estrus. Plasma progesterone concentrations of the 10 pregnant cows at 20 days after insemination were $5.82{\pm}1.33ng/ml$ (mean${\pm}$SD) and those of 10 non-pregnant cows were $0.30{\pm}0.21ng/ml$, respectively. Therefore, plasma progesterone concentrations in the pregnant cows at 20 days after insemination were significantly higher than in non-pregnant cows (p<0.001).

한우(韓牛)의 번식효율(繁殖效率) 증진(增進)에 관한 연구(硏究) -Progesterone농도(濃度) 측정(測定)에 의한 조기임신진단(早期姙娠診斷)-

강병규,최한선,이정길,손창호,서국현,Kang, Byong-kyu,Choi, Han-sun,Lee, Chung-gill,Chang-ho, Son,Suh, Guk-hyun 대한수의학회 1990 大韓獸醫學會誌 Vol.30 No.2

Plasma progesterone concentrations were measured in 24 Korean native cows by a radioimmunoassay at 0, 6 and 20 days after insemination. As a result, 23 cows (95.8%) were judged to be in estrus with ovulation and one cow (4.2%) was judged to be in estrus without ovulation. Plasma progesterone concentrations at 20 days after insemination were $5.11{\pm}1.33$ (mean${\pm}$SD, range 3.20~7.86) ng/ml in pregnant cows and $0.35{\pm}0.10$ (range 0.30~0.50) ng/ml in nonpregnant cows. Of the 20 cows, 18 cows were confirmed the pregnant by rectal palpation at 60 days after insemination. The accuracy of the pregnancy diagnosis based on plasma progesterone concentration was 90 % for positive case and 100% for negative case.

발정유기(發情誘起)된 암캐의 혈중(血中) progesterone 농도변화(濃度變化)

강병규,최한선,나진수,이차범,오기석,손창호,Kang, Byong-kyu,Choi, Han-sun,Na, Jin-su,Lee, Cha-bum,Oh, Ki-seok,Son, Chang-ho 대한수의학회 1989 大韓獸醫學會誌 Vol.29 No.3

This study was performed to investigate the patterns of progesterone secretion after induction of estrus in premature, metestrous and anestrous bitches. A total of 22 bitches were used. Of them 18 bitches were treated with hormone to induced estrus and 4 bitches were untreated and served as controls. Estrus was induced with $PGF_{2{\alpha}}$, estrone, estradiol-$17{\beta}$, PMSG and HCG(Treatment A), and with PMSG and HCG(Treatment B). Blood samples were collected via the cephalic vein at 2 to 5 days interval. Blood samples were centrifuged (1,200g, 10min.) within 30 minutes after collection and plasma was stored at $-20^{\circ}C$ until analyzed for the progesterone concentrations. Plamsa progesterone concentrations were measured by radioimmunoassay. The results of estrous induction were determined by estrous signs, ovarian response, egg recovery and progesterone patterns. The results obtained were as follows; 1. All bitches in treatment A showed estrous signs, however the ovarian response and egg recovery were not detectable and the levels of progesterone were nearly same as before. 2. In the treatment B, premature and metestrous bitches showed only estrous signs, however 5 of 7 anestrous bitches (71.4%) showed estrous signs, ovarian response and changes of progesterone levels. In conclusion, clinical estrous behavior can be induced during any phase of the estrous cycle, but ovulation should be induced only if induction occur approximately 4 months or more after the previous estrus.

발정유기견(發情誘起犬)의 난소반응(卵巢反應)과 수정란(受精卵)의 외과적(外科的) 회수(回收)

강병규,최한선,나진수,오기석,손창호,이차범,Kang, Byong-kyu,Choi, Han-sun,Na, Jin-su,Oh, Ki-seok,Son, Chang-ho,Lee, Cha-bum 대한수의학회 1989 大韓獸醫學會誌 Vol.29 No.3

The aim of the present study was to test the efficiency of estrous induction in the premature, metestrous and anestrous bitches. The estrus was induced with prostaglandin $F_{2{\alpha}}$, estradiol-$17{\beta}$, pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin(HCG) in the treatment A, and with PMSG and HCG in the treatment B. Day 0 was the first day of estrone injection in the treatment A and the day of PMSG injection in the treatment B. Twenty three of the twenty six bitches were laparotomized under general anesthesia between 11 and 18 days after onset of behavioral estrus, whereas three bitches were not laparotomized and remained until parturition. Ovarian responses were evaluated with the total number of corpora lutea or ovulation sites. The uterine horns were flushed with phosphate-buffered saline added heat treated canine serum(10%), the flushing media was collected into watch glass and the ova were examined under stereomicroscope. The results obtained were as follows: 1. Standing estrus was observed on the day $17.7{\pm}1.5$ after injection of estrone in the treament A, but ovarian responses were not detectable. 2. Standing estrus was observed on the day $12.2{\pm}0.2$ after injection of PMSG in the treament Band 14 of 17 bitches showed ovarian responses. Ova were recovered in 9 of the 14 bitches. 3. Ovarian responses were observed in one of the three premature bitches. two of the three metestrous bitches and all of the 11 anestrous bitches. The average number of the ova collected from 9 bitchs were $12.2{\pm}1.4$. 4. Three bitches in the treament B exhibited behavioural estrus and all of them were mated with fertile male dog, resulting the pregnancy in only one bitch. The pregnant bitch gave the birth of two pups.

인공적(人工的) 발정유기견(發情誘起犬)의 질세포상(膣細胞像)에 관(關)한 연구(硏究)

강병규,최한선,나진수,손창호,오기석,이차범,Kang, Byong-kyu,Choi, Han-sun,Na, Jin-su,Son, Chang-ho,Oh, Ki-seok,Lee, Cha-bum 대한수의학회 1988 大韓獸醫學會誌 Vol.28 No.1

The present study was carried out to investigate the estrous behavior and vaginal smear after induction of estrus with exogenous hormones in the premature, metestrous and anestrous bitches. A total of 21 bitches(Mixed breed: 16, Jindo breed: 5), from 10 months to 5 years of age and weighing 8 to 15 kg was studied the change of vaginal smear and the estrous behavior before and after induction of estrus. The results obtained are as follows: 1. In the treatment A(They were given the $PGF_2{\alpha}$, estrone, estradiol-$17{\beta}$, PMSG and HCG) proestrus commenced in $10.16{\pm}1.44$($Mean{\pm}SEM$) days after treatment, The mean duration of proestrus, and estrus was $7.50{\pm}1.44$ and $13.50{\pm}3.44$ days, respectively. In the treatment B(They were given the PMSG and HCG) proestrus commenced in $5.53{\pm}0.59$ days after treatment. The mean duration of proestrus and estrus was $6.60{\pm}0.71$ and $14.60{\pm}1.14$ days, respectively. 2. All of the 6 bitches in the treatment A showed vulval swelling and vaginal discharge, 14 of the 15 bitches in the treatment B showed vulval swelling and vaginal discharge. However, all of the treatment A and B showed male acceptance. 3. The main change of vaginal smear in proestrus and estrus after induction of estrus was a increase in the proportion of anuclear and superficial cells associated with a decrease in small intermediate and parabasal cells. By the estrous behavior and vaginal smear the estrus was induced in all the premature, metestrous and anestrous bitches.

Bordetella 감염증(感染症)의 혈청학적진단(血淸學的診斷): 특히 보균돈검색(保菌豚檢索)을 위한 급속평판응집반응(急速平板凝集反應)의 실용화(實用化)

강병규,Kang, Byong Kyu 대한수의학회 1978 大韓獸醫學會誌 Vol.18 No.2

The detection of Bordetella bronchiseptica which is supposed to be an agent of the infectious atrophic rhinitis of swine, is likely to receive more attention in the future as the pork industry comes to realize that eradication of this infection from breeding herds is a practical possibility. Experiments described here were carried out to establish the rapid plate agglutination test for the detection of the infectious atrophic rhinitis of swine in the field using the criteria of antigen preparation, effects on the antigenecity after storing of the antigen and reaction appearing time. Also, the agglutinabilities between the plate and tube method were compared and the degree of pathological lesions were recorded in relation to tube agglutination titers. Obtained results were as follows: 1. No differences were noted in the agglutinabilities on the plate agglutination test between the treatments in antigen preparation-formolized, merthiolate-killed and living organism. 2. The agglutinability of the antigens did not show any significant changes until 10 weeks of storage at 4 C; however, after 10 weeks of storage, non-specific reaction was observed with the HPCD control sera. 3. The results of the plate and tube agglutination tests were not comparable but the effective use of the plate method in Bordetella bronchiseptica eradication programs in pigs especially in the sow is stressed as a screening test.

한국재래산양(韓國在來山羊)의 성성숙일령(性成熟日齡) 및 계절번식성(季節繁殖性)

강병규,최한선,박영준,박범준,손창호,Kang, Byong-kyu,Choi, Han-sun,Park, Young-jun,Park, Bum-jun,Son, Chang-ho 대한수의학회 1992 大韓獸醫學會誌 Vol.32 No.2

To investigate the age at puberty and the seasonal breeding in Korean native goats, progesterone concentrations were measured in blood. Blood samples were collected from 8 goats at 10 day intervals from 2 months of age until the first estrus after birth, and then every 5 days for a further estrous cycle and the seasonal breeding. The mean age and weight at puberty were $195{\pm}57$ days($mean{\pm}S.D.$, range : 107~260 days) and $11.1{\pm}0.9kg$(range : 9.8~12.0kg), respectively. The mean age at first pregnancy after birth was $241{\pm}109$ days(range : 107~273 days). The estrus was observed 47.6% from October to December, and was highest in fall(38.1%) and lowest in spring and summer(14.3%). However, the estrus was observed every season. About 67% of total conception occurred form October to January. The parturition occurred 41.7% in spring, 25.0% in summer and winter, and 8.3% in fall, respectively. These results suggest that Korean native goats do not have a breeding season, but the reproductive activity is influenced by the season.

전기전도도(電氣傳導度) 측정(測定)에 의한 유우(乳牛) 준임상형(準臨床型) 유방염(乳房炎)의 진단(診斷)에 관한 연구(硏究) 1. 전기전도도법(電氣傳導度法)과 간접검진법(間接檢診法)(CMT 및 총체세포수(總體細胞數))과의 비교(比較)

강병규,Kang, Byong-kyu 대한수의학회 1984 大韓獸醫學會誌 Vol.24 No.1

A total of 466 foremilk from dairy farms in Chonnam district was examined for the subclinical mastitis over a period of one year, using a method of the electrical conductivities(EC); absolute conductivity(AC) and differential conductivity(DC) and quarter difference value(QD), in relation to the California mastitis test(CMT) and the direct somatic cell count(DSCC). The compatibility and efficiency rating between the EC values and the other screening tests was conducted. Obtained results are summarized as follows. 1. A linear relationship was found between the EC values and the CMT scores and direct somatic cell counts and it was found that electrical conductivity measurements were comparable with other screening tests for diagnosing animals with mastitis. 2. Compatibilities between the EC and CMT were 70.4% in AC, 74.6% in DC and 70.7% in QD, and that of the EC and DSCC were 53.0% in AC, 63.1% in DC and 53.2% in QD. On the other hand, relative efficiency ratings of Postle's equation between EC and CMT were 37.3% in AC, 26.5% in DC and 13.6% in QD, and that of the EC and DSCC were 33.1% in AC, 20.2% in DC and 11.9% in QD. 3. In the foremilk samples collected from damaged quarters determined by EC, the false positive rate wart higher than the false negative rate, and consequently tests of EC produced lower compatibility or efficiency rating scores. These tendencies suggested that any factors other than the mastitic condition influencing the EC values might be existed.

Serologic Studies on Porcine Strains of Haemophilus parahaemolyticus (pleuropneumoniae) : Antigenic Specificity and Prevalence of Antibodies to Serotypes

강병규,산본 효사,미형 학,Kang, Byong-Kyu,Yamamoto, K.,Ogata, M. The Korean Society of Veterinary Science 1983 大韓獸醫學會誌 Vol.23 No.2

Haemophilus parahaemolyticus(pleuropneumoniae)균(菌)(HP균(菌)) 혈청(血淸) 1형(型)(4074주(株)), 2형(型)(1536주(株)), 3형(型)(1421주(株)) 및 5형(型)(966주(株))의 혈청반응특이성(血淸反應特異性)을 검토할 목적으로, 2주령(週齡)의 SPF돈(豚)에 HP균(菌) 신선분리주(新鮮分離株)(HP8주(株))를 경비접종(經鼻接種)하여 경시적(經時的)으로 나타나는 항체가(抗體價)를 주로 응집반응(凝集反應)(AG)과 보체결합반응(補體結合反應)(CF)으로 비교검토하였다. 아울러 야외(野外)에서 채취한 돈혈청(豚血淸)에 대하여 각 혈청형별(血淸型別) 항체가(抗體價)의 분포(分布)도 AG및 CF반응(反應)으로 조사하였다. 얻어진 결과(結果)를 요약하면 다음과 같다. 1. 응집항체(凝集抗體)(AG항체(抗體)) 및 보체결합항체(補體結合抗體)(CF항체(抗體))는 접종(接種)한 다음 1주일에 나타나기 시작하였고, AG항체(抗體)는 접종(接種) 2주후에 그리고 CF항체(抗體)는 2~3주후에 최고치를 나타내었다. 그리고 CF항체(抗體)는 AG항체(抗體)보다 약간 늦게 나타나는 경향이 인정되었다. 2. HP균(菌)(HP 8주(株))혈청(血淸) 2형감염돈(型感染豚) 혈청(血淸)에 대한 각 혈청항원간(血淸抗原間)의 반응(反應)에서는 항체가(抗體價)가 낮기는 하나 교차반응(交叉反應)이 안정되었고, 특히 혈청(血淸) 1형(型) 5형항원(型抗原)에서 이러한 현상이 인정되었다. 3. 야외혈청(野外血淸)에 대한 HP균(菌)의 혈청형별항체(血淸型別抗體)는 주로 HP균(菌)2형(型)이 대부분이었고, 그 검출양성율(檢出陽性率)은 AG반응(反應)에서 48.6%, CF반응(反應)에서 37.1%를 나타내고 있어, HP균혈청(菌血淸) 2형균(型菌)의 감염(感染)이 존재함이 혈청학적(血淸學的)으로 확인되었다. 4. HP균(菌)의 혈청반응(血淸反應)의 실시에 있어서는 AG반응(反應)에서 나타나는 비특이반응(非特異反應)은 가검혈청(可檢血淸)을 kaolin처리 함으로서 이를 배제할 수 있었고, CF반응(反應)에 있어서는 신선자우혈청(新鮮仔牛血淸)을 보체계(補體系)에 첨가함으로써 효과적인 반응을 실시할 수 있음이 확인되었다.