연속 2 단계 교반조 효소 반응장치의 최적화에 관한 연구

박영훈,유두영 한국화학공학회 1978 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.16 No.4

For production of 6-aminopenicillanic acid by enzymatic hydrolysis of benzylpenicillin, two-stage continuous stirred tank enzyme reactor system was evaluated. Using proper mathematical model of the reaction kinetics and kinetic constants of soluble and immobilized forms of penicillin amidohydrolase, the reactor system was simulated with an aid of a computer and a set of optimum operating conditions was found. In comparison with the single stage system, the productivity of the two-stage enzyme reactor system was 50% higher than that of the single stage system under the same operating conditions. For the industrial applications, the reactor system using immobilized penicillin amidohydrolase was found to be far more advantageous in terms of productivity than that using soluble enzyme because of the reduced inhibitory effect of the product, 6-aminopenicillanic acid, on the immobilized enzyme as compared to the same effect on the soluble enzyme.

생물소재관련 산업기술분야

박영훈 한국과학기술원 유전공학연구소 1993 생명공학동향 Vol.1 No.4

Copper Phthalocyanine 生成速度에 미치는 觸媒의 效果

朴永勳 尙志大學校 1981 論文集 Vol.2 No.-

Since a blue substance was discovered from a reaction of heating o-cyanobenzamide in 1907 extensive studies of phthalocyanine and it's metallic complexes have been undertaken by a number of research groups. In respect of synthesis there are generally three to routes lead phthalocyanine. Yet, none of mechanism of the formation has been investigated. Synthesis choosed in this study is Wyler's method using phthalic anhydride and urea as main starting meterials. The effect of catalysts on the reaction rate was examined. It is known, in general, that certain suitable catalysts accelerate the rate of ring formation of Cu-phthalocyanine, and the common ones for the purpose are ferric chloride, aluminium chloride, titanium tetrachloride and ammonium molybdate. The effectiveness of these catalysts for the formation of Cu-pc has investigated here by the kinetic method. The amount of titanium tetrachloride and ammonium molybdate used was one tenth as much as the others. And ammonium molybdate used was one tenth as much as the others. And ammonium molybdate is found to be the must effective among them all. Next the variation of optical density measured at definite intervals during Cu-pc formation from phthalic anhydride, urea and cupric chloride as starting and ammonium moybdate as a catalyst was substituted into the equation log(D∞-Do)/(D∞-Dt). The absorption maximum was at 440nm.

알루미나에 담지된 친핵성 시약과 할로겐화 알킬과의 반응 연구

朴永勳 상지대학교 1994 論文集 Vol.15 No.-

Recent organic reactions are summarized to publicize that reagent adsorbed on active metal oxide (i.e., alumina or silica gel) can be used deliberately to achive some very mild and highly selective organic transformations. Under this study, substitution of aliphatic alkyl halides using inorganic salts (i.e., LiN₃, NaN₃, LiSCN, NaSCN, KSCN, NaNO₂) adsorbed in monolayer on active alumina was conducted and the data on reactivity were compared. Specially, monothiocyanation of 1,3-dibromopropance with KSCN in the presence of PTC or alumina was compared. Further, these basic data will be search for the most effective reaction conditions for nucleophilic substitution reactions of alkyl halide with alumina-supported reagent.

Gospel Song과 함께(8)

박영훈 생명샘 1983 생명샘 Vol.- No.1

Azaquinazoline 誘導體의 合成에 關한 硏究

朴永勳 尙志大學校 1986 論文集 Vol.7 No.-

1,3-disubstituted-8-aza-quinazoline-2,4-dione derivatives were synthesized by the following methods using 2-chloronicotinic acid as starting material (call method A and method B in this paper). In method A 1-substituted-8-aza-quinazoline-2,4-diones were prepared by the reaction of 2-(N-substituted amino) nicotinic acid with urea at high temperature, while in method B 1-substituted-8-aza-quinazoline-2,4-diones prepared by the reaction of 2-(N-substituted amino)nicotinamide with ethyl carbonates at low temperature. The former requires only 2-3 hours of reaction time at 20-40℃, while the latter requires 7 hours at 180-200℃. Yields for two methods obtained were 60% all. It is clear that the method B is more efficient than the method A. Yields of 1,3-disubstituted-8-aza-quinazoline-2,4-dione as object was obtained about 60% by the reaction of intermediate material with alkylate in the DMF solvent. It has been affirmed that the mechanism of reactions involved in synthesis was of nucleophilic substitution. The molecular structure, and molecular weight of 1,3-disubstituted-8-aza-quinazoline-2,4-diones were confirmed by the analysis of physico-chemical instrumentation such as IR, NMR, and MS spectra, and the physical properties were examined. And the values of elementary analysis for C, H and N were in very good agreement with the expected results.

Azaquinazolinedione 誘導體의 合成

朴永勳 尙志大學校 1985 論文集 Vol.6 No.-

1, 3-Disubstituted-8-aza-quinazoline-2, 4-dione derivatives were synthesized by the following method A and B. Method A: 1-substituted-8-aza-quinazoline-2, 4-diones were prepared by the reaction of 2-(N-substituted amino) nicotinic acid with urea at high temperature. On the otherhand method B: 1-Substituted-8-aza-quinazoline-2, 4-diones prepared by the reaction of 2-(N-substituted amino) nicotinamide with ethyl carbonates at low temperature. The former requires only 2-3 hours of reaction time at 20-40 ℃, while the latter requires 7 hours at 180-200 ℃. Yields for two methods obtained were 60% all. It is clear that the method B is more efficient than the method A. Yields of 1, 3-disubstituted-8-aza-quinazoline-2, 4-dione as object was obtained about 60% by the reaction of intermediate material with alkyate in the DMF solvent. It has been affirmed that the mechanism of reactions involved in synthesis was of nucleophilic substitution. The molecular structure, and molecular weight of 1,3-disubstituted-8-aza-quinazoline-2, 4-diones were confirmed by the analysis of physico-chemical instrumentation such as IR, NMR, and MS spectra, and the physical properties were examined.

Phenazin 環을 가진 界面活性劑의 合成과 그 抗菌性(제2보)

朴永勳 尙志大學校 1983 論文集 Vol.4 No.-

7-Alkyl-1-hydroxyphenazine derivatives, bearing butyl or octyl group, were synthesized by the condensation reaction of pyrogallol-1-monoethylether with 3,4-diaminoalkylbenzene, prepared from aniline and alcohols as starting materials through alkylation of 3-nitro-4-aminoalkylbenzene. And the products were also identifed by elementary analysis, IR and NMR spectra. Ir spectra were determined in pressed potassium bromide disks. Nmr spectra were determined on a Varian spectrometer and shifts are reported in parts per million with TMS as an internal standard. Surface tension of these derivatives in aqueous solution was determined. And also antibiotic activity of these derivatives was tested, using Gram positive and negative bacillus and fungi. It showed that the antibiotic activity of these alkyl-substituted 1-hydroxyphenazine derivatives was stronger than that of the 1-hydroxyphenazine.

정방형내에서 경사냉각면이 동결현상에 미치는 영향에 관한 연구

박영훈,김병철 조선대학교 에너지.자원신기술연구소 1998 에너지·자원신기술연구소 논문지 Vol.20 No.1

It was studied on the phenomena of freezing with inclination cooling surfaces in the cube One side of the test section was cooled and the other sides were insulated The effects for the initial temperature, the inclination angle on the temperature field and the shape of the ice-water interface were observed In the beginning of freezing, with increasing the inclination angle the freezing rate was increased and in the stable density layers the convective fluid flow became small, the freezing was fast In the region above the 4℃ of initial temperature, the frozen thickness in the inclined surface of the upper part was thinner than that of lower part, but with time the frozen thickness of upper part was thicker than that of lower part, and also below the 4℃ of initial temperature the frozen thickness in the upper part was thicker than that of lower part from the beginning. 기호설명 H Height of enclosure GREEKS L length of enclosure η Dimensionless vertical coordinate [y/H] T Temperature ε Dimensionless horizontal coordinate [x/L] x Horizontal coordination SUBSCRIPTS y Vertical coordination i inital t Time c cold θ Angle of cooling surface

LiBr와 CaBr₂가 흡착된 알루미나상에서 일어나는 염화 알킬의 브롬화반응

박영훈,신영문,조범준,김창배,Park, Yeong Hoon,Shin, Young Mun,Cho, Beom Jun,Kim, Chang Bae 한국공업화학회 1996 공업화학 Vol.7 No.5

알루미나에 LiBr와 $CaBr_2$를 각각 흡착시켜 용매가 없는 상태에서 여러 가지 염화 알킬과 반응시켜 좋은 수득률로 해당하는 브롬화 생성물을 얻을 수 있었다. 이러한 높은 반응성은 알루미나에 흡착되어 있는 극소량의 물과 브롬화 금속간의 상호작용이 알루미나 상에서 일어날 수 있기 때문이라는 것을 Differential scanning calorimetry (DSC) 열분석 실험과 문헌을 통하여 고찰하였다. Several alkyl chlorides were brominated using LiBr and $CaBr_2$ adsorbed on alumina in the absence of solvent. The yields of alkyl bromides were fairly high. Such a high reactivity by these bromide salts might be due to the interaction of minute amount of water and the salt on alumina. Differential scanning calorimetry (DSC) thermogram was provided and discussed.