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사람의 배양세포에 있어 자외선 및 MMS 에 의한 비동시 (非同時) DNA 합성과 염색체 이상에 미치는 감기상사체의 (監基相似體) 영향
엄경일,박상대 ( Kyung Il Um,Sang Dai Park ) 한국유전학회 1981 Genes & Genomics Vol.3 No.1
The effects of base analogs on UV-light and MMS-induced unscheduled DNA synthesis and chromosome aberrations were investigated in human cells and the results obtained were as follows: BUdR and IUdR were found to be both potent sensitizers enhancing UV-induced unscheduled DNA synthesis and chromosome aberrations. The sensitization effects of these base analogs were different in these two different repair processes. BUdR and IUdR did not seem to act as sensitizers for both unscheduled DNA synthesis and chromosome aberrations induced by MMS. These results suggest that excision repair may not be related to chromosome aberrations in human cells.
鮮于 洋一,嚴慶一 東亞大學校 大學院 1981 大學院論文集 Vol.5 No.-
Isozymes and chromosomes of Kaloula borealis, hyla alborea japonica, and Bombina arientalis were studied by starch gel electrophoresis and bone marrow air-drying method. Twenty-three enzymatic proteins and enzymes of the three anuran species collected in Seoul province provided a basis for estimating the genetic distance among these species. On the genetic similariry, the relationship between Bombina orientalis and Kaloula alborea was nearer than the others. The chromosome number of Hyla alborea japonica and Bombina orientalis was 24, and Kaloula borealis was 28. The differences of these were a little.
포유동물 세포에서 고농도 돌연변이원에 의한 세포생존과 DNA 회복에 대한 저농도 돌연변이원의 전처리 효과
윤영삼,신은주,엄경일 東亞大學校附設基礎科學硏究所 1992 基礎科學硏究論文集 Vol.9 No.1
본 연구는 자외선과 ethyl methanesulfonate (EMS) 또는 bleomycin (BLM)을 처리한 Chinese hamster 난소세포에서 세포생존과 DNA 회복에 미치는 자외선의 전처리 효과를 밝히기 위하여, 세포생존 시험법과 알카리 유출법으로 조사하였다. 2J/㎡의 자외선을 전처리한 뒤 6시간 동안 배양한 후, 자외선 혹은 EMS를 처리한 군에서는 자외선 혹은 EMS 단독 처리군에 비해 세포생존이 증가하였다. 2J/㎡ 자외선을 전처리한 뒤 4시간 동안 배양한 후, 자외선 혹은 EMS를 처리한 군에서는 자외선이나 EMS를 단독 처리한 군에 비해 DNA 단사절이 증가 하였다. 이상의 결과들이 종합해 볼 때, 원핵 세포에서 알려진 적응 현상과 유사한 현상이 진핵생물에도 존재하는 것으로 추측된다. The effect of pretreatment with Ultraviolet (UV) radiation on cell survival and DNA repair in Chinese hamster ovary (CHO)-k₁cells treated with UV radiation, ethyl methanesulfonate (EMS), or bleomycin (BLM) was investigated. Two assays were employed in this study ; cell survival assay and alkaline elution. The survival of cells treated with UV-lights or EMS after incubation for 6 hours following pretreatment with 2 J/㎡ UV radiation was higher than the cell survival of UV alone or EMS treated group. DNA single-strand breaks induced by UV-irradiation or EMS after incubation for 4 hours following pretreatment with 2 J/㎡ UV radiation was higher than DNA single-strand breaks induced by UV-irradiation alone or EMS alone. The results suggest that there is a response in mammlian cells like adaptation processes in prokaryotic cells.
자외선-A를 조사한 CHO 세포의 생존에 대한 DNA 복제 효소 저해제의 영향
신은주,이재용,이동욱,엄경일 東亞大學校附設基礎科學硏究所 1995 基礎科學硏究論文集 Vol.12 No.1
본 연구는 동시화 하여 자외선-A를 조사한 Chinese hamster ovary (CHO) 세포의 세포생존에 미치는 novobiocin (NOV), aphidicolin (APC), 2', 3'-dideoxythymidine 5'-triphosphate (ddTTP) 및 3-aminobenzamide (3AB) 의 효과를 조사하였다. 세포의 동시화는 thymidine double block 방법을 사용하였다. 자외선-A에 노출 후 NOV, ddTTP, 3AB를 후처리하면 세포생존에 영향을 미치니 않으나, 자외선-A에 노출한 후 APC를 후처리 하면 세포의 모든 주기에서 세포생존이 감소하였고, 특히 G₁기에서 현저하게 감소하였다. 이상의 결과들은 DNA 중합효소 a가 자외선-A를 조사한 세포의 생존에 관여 하는 것으로 추측 된다. The present investigation has been performed to determine the effects of inhibitors, novobiocin (NOV), aphidicolin (APC), 2', 3' -dideoxythymidine 5'-triphosphate (ddTTP), and 3-aminobenzamide (3AB), of DNA replication enzymes on cell survival in synchronized Chinese hamster ovary (CHO)-K₁ cells exposed to ultraviolet-A radiation (UV-A). The cells were synchronized by the thymidine double block method. The survival of cells exposed to UV-A was not affected by post-treatment with NOV, ddTTP, and 3AB, while the post-treatment with APC decreased the survival of cells exposed to UV-A, and particularly is remarkable n G₁phase. These results suggest that the DNA polymerase a participate in the survival of cells exposed to UV-A.
HeLa 細胞에서 Bleomycin에 의해 誘發된 DNA 回復合成에 미치는 沮害劑의 影響
嚴慶一 東亞大學校 敎育大學院 1981 敎育大學院 論文集 Vol.7 No.-
HeLa S_3 세포를 재료로 DNA 회복합성에 미치는 Bleomycin(BLM)의 농도에 따른 효과와 저해제[5-aminouracil(AU), actinomycin D(AMD), caffeine (Caf), 5-fluorodeoxyuridine (FUdR)]들 각각의 단독효과, 그리고 BLM에 의한 DNA 합성 및 회복합성에 미치는 저해제들의 영향을 ^3H-thymidine 처리에 의한 자기방사법으로 조사한 결과는 다음과 같다. 1. BLM 단독 처리인 경우 BLM에 의해 유발되는 DNA 회복합성율은 1.0㎍/㎖의 농도까 농도의 증가에 따라 증가한다. 세포당 평균 grain 수로 정량 분석한 grain 수도 농도의 증가에 따라 1.0㎍/㎖의 농도까지 증가한다. 2. 저해제들을 단독으로 각각 처리할 경우 DNA 회복합성에 영향을 주지 않는다. 3. BLM과 저해제를 복합 처리한 모든 군의 DNA 회복합성율은 BLM 단독 처리군에 대해 변화가 없다. 그러나 BLM과 AU, BLM과 FUdR을 처리한 군에서는 DNA 합성이 억제되었다. 이상의 결과들은 본 실혐 조건 하에서 BLM에 의해 유발된 DNA 회복합성에 저해제들이 영향을 미치지 않는 것으로 추측된다. The present study has been undertaken to determine the effects of inhibitors [5-amin-ouracil (AU), actinomycin D (AMD), caffeine (Caf) and 5-fluorodeoxyuridine (FUdR)] on bleomycin (BLM) induced DNA synthesis was measured by autoradiography. The results obtained were as follows: 1. In the BLM treated group, the induced DNA repair synthesis by BLM was shown to in direct proportion upto 1.0 ㎍/㎖. The quantitative analysis of unscheduled DNA synthesis as determined by the average number of grains per cell was also shown that the grains were increased directly proportional to dose increased upto 1.0 ㎍/㎖. 2. The single treatment with inhibitors did not affect DNA repair synthesis. 3. The percentage of DNA repair synthesized cells did not significantly change in all BLM plus inhibitors as compared with the corresponding single BLM, but the normal DNA synthesis by BLM plus AU or FUdR was slightly suppressed. The above results suggested that these inhibitors did not seem to induce the BLM induced DNA repair synthesis under this experimental condition.
DNA 상해와 회복기작에 미치는 환경성 돌연변이원의 영향
엄경일,채기수,이주혜,정정남,이천복 한국유전학회 1986 Genes & Genomics Vol.8 No.4
The effects of aphidicolin, an inhibitor of DNA polymerase α, on ethyl methanesulfonate (EMS)- or bleomycin (BLM)-induced DNA repair were investigated in Chinese hamster ovary (CHO) cells. The amount of unscheduled DNA synthesis induced by EMS or BLM was increased according to dose increment and decreased with incubation time after treatment, and the damage almost seemed to be recovered during 4 hour incubation. The EMS-induced DNA single-strand breaks showed dose dependent increment and were increased according to EMS containing time. BLM also showed dose dependent increment on DNA single-strand breaks. On the other hand, it was shown that aphidicolin inhibited excision repair induced by EMS, and thus, the post-treatment with aphidicolin induced the higher degree of unscheduled DNA synthesis and the more accumulation of DNA single-strand breaks than the cells post-incubated without aphidicolin. However, aphidicolin did not inhibit excision repair induced by BLM, and thus, the cells post-incubated with or without aphidicolin for 1 hour had the same value in the amount of unscheduled DNA synthesis and of DNA single-strand breaks. These results suggested that the participation of DNA polymerase α in DNA repair induced by EMS or BLM was dependent on different types of damage.
균체외단백질 생산균 Bacillus brevis P176-2 균주의 동정과 배양조건
채기수,엄경일 東亞大學校大學院 1991 大學院論文集 Vol.16 No.-
Identification and influence of the cultural conditions upon extracellular protein production by Bacillus brevis P176-2 isolated from soil were investigated. The morphological, physiological and cultural characteristics of strain P176-2 were studied according to the methods of Bergey's manual. Based on the results obtained in thses experiments, strain P176-2 was identified as Bacillus brevis. The extracellular protein ws efficiently produced when added to 1.0% peptone as nitrogen source. Optimum initial pH and temperature for extracellular protein production were 7.5 and 40℃ respectively. Higher concentration of magnesium ion stimulated growth, but prevented extracellular protein production. Optimum concentration of potassium phosphate was found to be 0.2%. This strain produced 5.38mg/ml of extracellular protein when cultured for 48 hours under optimum conditions. The extracellular protein produced under optimum conditions contained relatively large amounts of aspartic acid, lysine and arginine, but cystine and methionine were very low.