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Proteomics Analysis of Gastric Epithelial AGS Cells Infected with Epstein-Barr Virus
Ding, Yong,Li, Xiao-Rong,Yang, Kai-Yan,Huang, Li-Hua,Hu, Gui,Gao, Kai Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1
Effects of the Epstein-Barr virus (EBV) on cellular protein expression are essential for viral pathogenesis. To characterize the cellular response to EBV infection, differential proteomes of gastric epithelial AGS cells were analyzed with two-dimensional gel electrophoresis (2-DE) followed by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) and liquid chromatography electrospray/ionization ion trap (LC-ESI-IT) mass spectrometry identification. Mass spectrometry identified 9 altered cellular proteins, including 5 up-regulated and 4 down-regulated proteins after EBV infection. Notably 2-DE analysis revealed that EBV infection induced increased expression of heat shock cognate 71 kDa protein, actin cytoplasmic 1, pyridoxine-5'-phosphate oxidase, caspase 9, and t-complex protein 1 subunit alpha. In addition, EBV infection considerably suppressed those cellular proteins of zinc finger protein 2, cyclin-dependent kinase 2, macrophage-capping protein, and growth/differentiation factor 11. Furthermore, the differential expressional levels of partial proteins (cyclin-dependent kinase 2 and caspase 9) were confirmed by Western blot analysis.Thus, this work effectively provided useful protein-related information to facilitate further investigation of the mechanisms underlying EBV infection and pathogenesis.
Jian Liu,Gui-jie Yang,Yong Li,Hong-wei Gao,Jian-yong Su 전력전자학회 2014 JOURNAL OF POWER ELECTRONICS Vol.14 No.1
To insure stable operation and eliminate twice torque ripple, a topology for a six phase permanent magnet synchronous generator (SP-PMSG) with a neutral point connected together was analyzed in this paper. By adopting an extended transformation matrix, the mathematic model of the space vector control was established. The voltage and torque equations were deduced while considering the third harmonic flux and inductance. In addition, the suppression third harmonic method and the closed loop control strategy were proposed. A comparison analysis indicates that the cooper loss minimum method and the current magnitude minimum method can meet different application requirements. The voltage compensation amount for each of the methods was deduced which also takes into account the third harmonic effect. A simulation and experimental result comparison validates the consistency through theoretical derivation. It can be seen that all of the two control strategies can meet the requirements of post-fault.
Zhang, Bai-Hua,Cheng, Gui-Yu,Xue, Qi,Gao, Shu-Geng,Sun, Ke-Lin,Wang, Yong-Gang,Mu, Ju-Wei,He, Jie Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3
Background: Basaloid squamous cell carcinoma of the esophagus (BSCCE) is a rare and distinctive tumor with no standard treatment. This study aimed to explore treatment in relation to prognosis of the disease. Methods: A total of 142 patients with BSCCE that underwent treatment in our hospital from March 1999 to July 2010 were retrospectively analyzed. All patients received surgery, 42 postoperative radiotherapy and 28 patients chemotherapy. Results: There were 26 patients included in stage I, 60 in stage II, 53 in stage III and 3 in stage IV. The clinical symptoms and macroscopic performances of BSCCE did not differ from those of typical esophageal squamous cell carcinoma. Among 118 patients receiving endoscopic biopsy, only 12 were diagnosed with BSCCE. The median survival time (MST) of the entire group was 32 months, with 1-, 3- and 5-year overall survival (OS) of 81.4%, 46.8% and 31.0%, respectively. The 5-year OS of stage I and II patients was significantly longer than that of stages III/IV, at 60.3%, 36.1% and 10.9%, respectively (p<0.001, p=0.001). The MST and 5-year OS were 59.0 months and 47.4% in patients with tumors located in the lower thoracic esophagus, and 27.0 months and 18.1% in those with lesions in the upper/middle esophagus (p=0.002). However, the survival was not significantly improved in patients undegoing adjunctive therapy. Multivariate analysis showed TNM stage and tumor location to be independent prognostic factors. Furthermore, distant metastasis was the most frequent failure pattern, with a median recurrence time of 10 months. Conclusion: BSCCE is an aggressive disease with rapid progression and a propensity for distant metastasis. It is difficult to make a definitive diagnosis via preoperative biopsy. Multidisciplinary therapy including radical esophagectomy with extended lymphadenectomy should be recommended, while the effectiveness of radiochemotherapy requires further validation for BSCCE.
Liu, Jian,Yang, Gui-Jie,Li, Yong,Gao, Hong-Wei,Su, Jian-Yong The Korean Institute of Power Electronics 2014 JOURNAL OF POWER ELECTRONICS Vol.14 No.1
To insure stable operation and eliminate twice torque ripple, a topology for a six phase permanent magnet synchronous generator (SP-PMSG) with a neutral point connected together was analyzed in this paper. By adopting an extended transformation matrix, the mathematic model of the space vector control was established. The voltage and torque equations were deduced while considering the third harmonic flux and inductance. In addition, the suppression third harmonic method and the closed loop control strategy were proposed. A comparison analysis indicates that the cooper loss minimum method and the current magnitude minimum method can meet different application requirements. The voltage compensation amount for each of the methods was deduced which also takes into account the third harmonic effect. A simulation and experimental result comparison validates the consistency through theoretical derivation. It can be seen that all of the two control strategies can meet the requirements of post-fault.
Chao Wang,Chunlan Yan,Yong-Gui Gao,Lian-Hui Zhang 한국미생물학회 2015 The journal of microbiology Vol.53 No.9
The quorum quenching system of Agrobacterium tumefaciens is specifically activated upon entering the stationary phase. Evidence has shown that this system includes two key components: the IclR-type transcriptional factor AttJ (also named as BlcR) and the AHL-lactonase AttM (also named as BlcC). At exponential phase, AttJ binds to the promoter region of attM and thus suppresses the expression of attM. At stationary phase, however, the small molecule SSA directly binds to AttJ and relieves its inhibition of AttJ and thereby triggers the expression of attM. While the regulation of AttM has been extensively investigated, little is known about the regulation of AttJ. In this study, we demonstrated the D101 amino acid of AttJ is essential for the AttJ function. In vitro, the variant protein of AttJD101H appeared to be readily aggregated. In vivo, the D101H mutation in AttJ entirely abolished the inhibitory activity of AttJ and overexpressed attM in A. tumefaciens A6. In addition, D101H mutation led to an overexpression of attJ, indicating an auto-regulatory mechanism for the attJ regulation. Put together, these findings demonstrate that D101 is an important amino acid for the transcription activity of AttJ and the transcription of attJ is regulated by a negative feedback loop. These results expand previous biochemical characterization of AttJ and provide new mechanistic insights into the regulation of quorum quenching in A. tumefaciens.
Deep removal of sulfur from real diesel by catalytic oxidation with halogen-free ionic liquid
Dan Liu,Jianzhou Gui,Yong-Ki Park,Shuang Yang,Yuhuan Gao,Xilai Peng,Zhaolin Sun 한국화학공학회 2012 Korean Journal of Chemical Engineering Vol.29 No.1
A halogen-free task-specific ionic liquid (TSIL) of 1-(2-carboxylic acid) ethyl-3-methylimidazolium bisulfate ([(CH2)2COOHmim] [HSO4]) is applied for deep oxidative desulfurization of real diesel as a catalyst and reaction media. The sulfur level of real diesel can be reduced from 200 to 20.5 μg/g at room temperature with small amount of ionic liquid using H2O2 as an oxidant. The used ionic liquid can be easily recycled and no obvious decrease in catalytic activity can be found after recycling five times.
Guan Yi-Xin,Pan Hai-Xue,Gao Yong-Gui,Yao Shan-Jing,Cho Man-Gi The Korean Society for Biotechnology and Bioengine 2005 Biotechnology and Bioprocess Engineering Vol.10 No.2
A size exclusion chromatography (SEC) process, in the presence of denaturant in the refolding buffer was developed to refold recombinant human $interferon-\gamma$ ($rhIFN-\gamma$) at a high concentration. The $rhlFN-\gamma$ was overexpressed in E. coli resulting in the formation of inactive inclusion bodies (IBs). The IBs were first solubilized in 8 M urea as the denaturant, and then the refolding process performed by decreasing the urea concentration on the SEC column to suppress protein aggregation. The effects of the urea concentration, protein loading mode and column height during the refolding step were investigated. The combination of the buffer-exchange effect of SEC and a moderate urea concentration in the refolding buffer resulted in an efficient route for producing correctly folded $rhIFN-\gamma$, with protein recovery of $67.1\%$ and specific activity up to $1.2\times10^7\;IU/mg$.
Shan-Jing Yao,Yi-Xin Guan,Hai-Xue Pan,Yong-Gui Gao,Man-Gi Cho 한국생물공학회 2005 Biotechnology and Bioprocess Engineering Vol.10 No.2
A size exclusion chromatography (SEC) process, in the presence of denaturant in the refolding buffer was developed to refold recombinant human interferon- (rhIFN-) at a high concentration. The rhIFN- was overexpressed in E. coli, resulting in the formation of inactive inclusion bodies (IBs). The IBs were first solubilized in 8 M urea as the denaturant, and then the refolding process performed by decreasing the urea concentration on the SEC column to suppress protein aggregation. The effects of the urea concentration, protein loading mode and column height during the refolding step were investigated. The combination of the buffer-exchange effect of SEC and a moderate urea concentration in the refolding buffer resulted in an efficient route for producing correctly folded rhIFN-, with protein recovery of 67.1% and specific activity up to 1.2 107 IU/mg.