English Paper Presentation 1: Resuscitation/Airway Management : OE2-4 ; Protective Effect of Adiponectin on Paraquat-Induced Pulmonary Fibrosis in Mice

( Rong Yao ),( Ya Rong He ),( Zong An Liang ),( Zhi Zeng ),( Yu Cao ) 대한응급의학회 2014 대한응급의학회 학술대회초록집 Vol.2014 No.2

Pulmonary fibrosis is one of the most common complications of paraquat (PQ) poisoning which accounts for high mortality. Accumulating evidence suggest adiponectin (APN), an adipocytokine predominantly secreted by adipocytes, maybe a promising therapeutic option in fibrotic diseases. Here we aim to evaluate the potential effect and mechanism (s) of exogenous globular form of APN on PQ-induced pulmonary fibrosis. Eighty BALB/C mice were divided into four groups (control group, PQ intoxication group, PQ + low dose APN group and PQ + high dose APN group) and sacrificed on days 3, 7, 14 and 21. Lung tissues were collected and analyzed histologically and biochemically. Human lung fibroblasts WI-38 were divided into three groups (control group, PQ intoxication group and APN group) and collected for measurements at 24, 48 and 72 h after PQ stimulation. HE and Masson trichrome staining were used to observe the histological alterations of lungs. The Ashcroft score of lungs were also measured. Cell apoptosis was measured by Annexin V-FITC/PI double labeling. Dihydroethidium (DHE) was used to detect intracellular O2-. The protein and mRNA expression level of TGF-β1, NF-κB p65,α -SMA and IL-1Ra were assessed by Western blot and Real-time quantitative flouorescence-PCR. APN administration significantly ameliorated PQ mediated fibrotic histological alterations and reduced the protein and mRNA expression of TGF-β1 and NF-κB p65 in lung tissues of mice in a dose-dependent manner. Also, Pretreated with APN down-regulated the expression of α -SMA, O2- and NF-κB p65 in WI-38 lung fibroblasts exposure to PQ, while up-regulated the expression of anti-inflammatory factor IL-1Ra. Taken together, this study reveals the beneficial effect of APN against PQ-induced pulmonary fibrosis in a dose-dependent manner, which may through suppressing NF-κB dependent inflammatory and TGF-β1 mediated fibrotic events.

GRP78 Secreted by Colon Cancer Cells Facilitates Cell Proliferation via PI3K/Akt Signaling

Fu, Rong,Yang, Peng,Wu, Hai-Li,Li, Zong-Wei,Li, Zhuo-Yu Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17

Glucose regulated protein 78 (GRP78) is usually recognized as a chaperone in the endoplasmic reticulum. However, increasing evidence indicates that GRP78 can be translocated to the cell surface, acting as a signaling receptor for a variety of ligands. Since little is known about the secretion of GRP78 and its role in the progression of colon cancer we here focused on GRP78 from colon cancer cells, and purified GRP78 protein mimicking the secreted GRP78 was able to utilize cell surface GRP78 as its receptor, activating downstream PI3K/Akt and Wnt/${\beta}$-catenin signaling and promote colon cancer cell proliferation. Our study revealed a new mode of action of autocrine GRP78 in cancer progression: secreted GRP78 binds to cell surface GRP78 as its receptor and activates intracellular proliferation signaling.

Comparison of Chlorophyll Algorithms in the Bohai Sea of China

Xiu, Peng,Liu, Yuguang,Rong, Zengrui,Zong, Haibo,Li, Gang,Xing, Xinogang,Cheng, Yongcun The Korean Society of Oceanography 2007 Ocean science journal Vol.42 No.4

Empirical band-ratio algorithms and artificial neural network techniques to retrieve sea surface chlorophyll concentrations were evaluated in the Bohai Sea of China by using an extensive field observation data set. Bohai Sea represents an example of optically complex case II waters with high concentrations of colored dissolved organic mattei (CDOM). The data set includes coincident measurements of radiometric quantities and chlorophyll a concentration (Chl), which were taken on 8 cruises between 2003 and 2005, The data covers a range of variability in Chl in surface waters from 0.3 to 6.5 mg $m^{-3}$. The comparison results showed that these empirical algorithms developed for case I and case II waters can not be applied directly to the Bohai Sea of china, because of significant biases. For example, the mean normalized bias (MNB) for OC4V4 product was 1.85 and the root mean square (RMS) error is 2.26.

Preparation and Antitumor Activity of a Tamibarotene-Furoxan Derivative

Wang, Xue-Jian,Duan, Yu,Li, Zong-Tao,Feng, Jin-Hong,Pan, Xiang-Po,Zhang, Xiu-Rong,Shi, Li-Hong,Zhang, Tao Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

Multi-target drug design, in which drugs are designed as single molecules to simultaneously modulate multiple physiological targets, is an important strategy in the field of drug discovery. QT-011, a tamibarotene-furoxan derivative, was here prepared and proposed to exert synergistic effects on antileukemia by releasing nitric oxide and tamibarotene. Compared with tamibarotene itself, QT-011 displayed stronger antiproliferative effects on U937 and HL-60 cells and was more effective evaluated in a nude mice U937 xenograft model in vivo. In addition, QT-011 could release nitric oxide which might contribute to the antiproliferative activity. Autodocking assays showed that QT-011 fits well with the hydrophobic pocket of retinoic acid receptors. Taken together, these results suggest that QT-011 might be a highly effective derivative of tamibarotene and a potential candidate compound as antileukemia agent.

Comparison of Chlorophyll Algorithms in the Bohai Sea of China

Peng Xiu,Yuguang Liu,Zengrui Rong,Haibo Zong,GangLi,Xiaogang Xing,Yongcun Cheng 한국해양과학기술원 2007 Ocean science journal Vol.42 No.4

Empirical band-ratio algorithms and artificial neural network techniques to retrieve sea surface chlorophyll concentrations were evaluated in the Bohai Sea of China by using an extensive field observation data set. Bohai Sea represents an example of optically complex case II waters with high concentrations of colored dissolved organic matter (CDOM). The data set includes coincident measurements of radiometric quantities and chlorophyll a concentration (Chl), which were taken on 8 cruises between 2003 and 2005. The data covers a range of variability in Chl in surface waters from 0.3 to 6.5 mg m-3. The comparison results showed that these empirical algorithms developed for case I and case II waters can not be applied directly to the Bohai Sea of China, because of significant biases. For example, the mean normalized bias (MNB) for OC4V4 product was 1.85 and the root mean square (RMS) error is 2.26.

Hypoxia-induced circRNF13 promotes the progression and glycolysis of pancreatic cancer

Zhao Qiuyan,Zhu Zhonglin,Xiao Wenqin,Zong Guanzhao,Wang Chuanyang,Jiang Weiliang,Li Kai,Shen Jie,Guo Xingya,Cui Jianhua,Guo Lihong,Wan Rong 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Pancreatic cancer (PC) is one of the most malignant tumors. Rapid progression and distant metastasis are the main causes of patient death. Hypoxia is a hallmark of multiple cancers and is involved in tumor biology. However, little is known about the roles of circRNAs in glycolysis and hypoxia-mediated progression of PC. Here, the expression pattern of hypoxia-related circRNAs was analyzed using RNA sequencing. A unique circRNA termed circRNF13 was found to be upregulated in PC tissues and may be a potential prognostic indicator. HIF-1α and EIF4A3 are involved in regulating the biogenesis of circRNF13. Furthermore, circRNF13 was validated to exert a stimulative effect on cell proliferation, angiogenesis, invasion and glycolysis. Importantly, we found that circRNF13 promoted PDK3 levels by acting as a miR-654-3p sponge, thus promoting the PC malignant process. Collectively, our results reveal that hypoxia-induced circRNF13 mediated by HIF-1α and EIF4A3 promotes tumor progression and glycolysis in PC, indicating the potential of circRNF13 as a prognostic biomarker and therapeutic target for PC.

Direct Position Determination of Coherently Distributed Sources based on Compressed Sensing with a Moving Nested Array

( Zhang Yankui ),( Xu Haiyun ),( Ba Bin ),( Zong Rong ),( Wang Daming ),( Li Xiangzhi ) 한국인터넷정보학회 2019 KSII Transactions on Internet and Information Syst Vol.13 No.5

The existing direct position determinations(DPD) for coherently distributed(CD) sources are mostly applicable for uniform linear array(ULA), which result in a low degree of freedom(DOF), and it is difficult for them to realize the effective positioning in underdetermined condition. In this paper, a novel DPD algorithm for coherently distributed sources based on compressed sensing with a moving nested array is present. In this algorithm, the nested array is introduced to DPD firstly, and a positioning model of signal moving station based on nested array is constructed. Owing to the features of coherently distributed sources, the cost function of compressed sensing is established based on vectorization. For the sake of convenience, unconstrained transformation and convex transformation of cost functions are carried out. Finally, the position coordinates of the distribution source signals are obtained according to the theory of optimization. At the same time, the complexity is analyzed, and the simulation results show that, in comparison with two-step positioning algorithms and subspace-based algorithms, the proposed algorithm effectively solves the positioning problem in underdetermined condition with the same physical element number.

β-catenin protein utilized by Tumour necrosis factor-α in porcine preadipocytes to suppress differentiation

( Xiao Luo ),( Hui Xia Li ),( Rong Xin Liu ),( Zong Song Wu ),( Ying Juan Yang ),( Gong She Yang ) 생화학분자생물학회 2009 BMB Reports Vol.42 No.6

Diagnostic Value of ¹⁸F-FDG PET/CT in Comparison to Bone Scintigraphy, CT and ¹⁸F-FDG PET for the Detection of Bone Metastasis

Liu, Ning-Bo,Zhu, Lei,Li, Ming-Huan,Sun, Xiao-Rong,Hu, Man,Huo, Zong-Wei,Xu, Wen-Gui,Yu, Jin-Ming Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6

Purpose: To evaluate the diagnostic value of $^{18}F$-FDG PET/CT for detection of bone metastasis in comparison with the efficacies of $^{18}F$-FDG PET/CT, CT, $^{18}F$-FDG PET and conventional planar bone scintigraphy in a series of cancer patients. Methods: Five hundred and thirty patients who underwent both $^{18}F$-FDG PET/CT and bone scintigraphy within 1 month were retrospectively analyzed. The skeletal system was classified into 10 anatomic segments and interpreted blindly and separately. For each modality, the sensitivity, specificity, accuracy, PPV and NPV were calculated and the results were statistically analyzed. Results: Bone metastases were confirmed in 117 patients with 459 positive segments. On patient-based analysis, the sensitivity, specificity, accuracy, PPV and NPV of $^{18}F$-FDG PET/CT were significantly higher than bone scintigraphy, CT and $^{18}F$-FDG PET (P<0.05). On segment-based analysis, the sensitivity of CT, bone scintigraphy, $^{18}F$-FDG PET and $^{18}F$-FDG PET/CT were 70.4%, 89.5%, 89.1% and 97.8%, respectively (P<0.05, compared with $^{18}F$-FDG PET/CT). The overall specificity and accuracy of the four modalities were 89.1%, 91.8%, 90.3%, 98.2% and 90.3%, 90.9%, 89.8%, 98.0%, respectively (P<0.05, compared with $^{18}F$-FDG PET/CT). The PPV and NPV were 89.8%, 87.6%, 85.6%, 97.2% and 85.6%, 93.2%, 92.8%, 98.6%, respectively. Three hundred and twelve lesions or segments were presented as lytic or sclerotic changes on CT images at the corresponding sites of increased $^{18}F$-FDG uptake. In lytic or mixed lesions, the sensitivity of $^{18}F$-FDG PET/CT and $^{18}F$-FDG PET were better than bone scintigraphy, while in osteoblastic lesions bone scintigraphy had a similar performance with $^{18}F$-FDG PET/CT but better than $^{18}F$-FDG PET alone. Conclusion: Our data allow the conclusion that $^{18}F$-FDG PET/CT is superior to planar bone scintigraphy, CT or $^{18}F$-FDG PET in detecting bone metastasis. $^{18}F$-FDG PET/CT may enhance our diagnosis of tumor bone metastasis and provide more information for cancer treatment.

Direct ethanol production from dextran industrial waste water by Zymomonas mobilis

Ming-xiong He,Han Qin,Xiao-bo Yin,Zhi-yong Ruan,Fu-rong Tan,Bo Wu,Zong-xia Shui,Li-chun Dai,Qi-chun Hu 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.11

The direct production of ethanol from dextran industrial waste water was investigated by using Zymomonasmobilis via batch and semi-continuous fermentation mode. In batch fermentation, pretreated waste water (unsterilizedand sterilized), pH value (3.8 and 6.0), and Mg2+(with and without) was compared with OD600, sugar and ethanol con-centration. After 24 h fermentation, sugar in the dextran waste water was almost exhausted, and the amount of ethanolaccumulated reached 24.33-29.92 g/l, which is nearly 99% of the theoretical yield of ethanol. Kinetic parameters ofZ. mobilis in batch fermentation were also investigated. The raw dextran waste water was also used in semi-continuousfermentation. After 48 h fermentation, the production of ethanol was 28.65 g/l. These results indicated that dextranwaste water may be used as a candidate substrate and Z. mobilis could convert the raw material into ethanol directly.