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Intracellular Uptake of Magnetite Nanoparticles Conjugated with RGDS-Peptide
Zhi-Cai Xing,강인규,박민정,한승진,최문정,이병헌,장용민 한국고분자학회 2011 Macromolecular Research Vol.19 No.9
Peptides containing the Arg-Gly-Asp (RGD) motif inhibit cell adhesion and exhibit a range of other biological effects including anticoagulant and antimetastatic activities. This study examined the anchorage independent effects of an RGD-containing peptide, Arg-Gly-Asp-Ser (RGDS), on NIH 3T3 fibroblasts. Magnetite nanoparticles were prepared by the coprecipitation of ferrous iron (Fe^2+) and ferric iron (Fe^3+) with NH4OH and dextran (DNPs). The aminosilane agent of 3-aminopropyltriethoxysilane (A) is considered to be a candidate for modification of the surface of magnetite nanoparticles (ADNPs). The amino-functionalized magnetite nanoparticles were activated using the glutaraldehyde (G) method (GADNPs). Subsequently, RGD or RGDS peptides were immobilized on the glutaraldehyde-modified magnetite nanoparticles (RGADNPs or RSGADNPs). The RSGADNPs were evaluated using Fourier-transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), and dynamic light scattering (DLS). The biological responses of the RSGADNPs were evaluated using NIH 3T3 fibroblast cells. Inductively coupled plasma mass spectrophotometry (ICP-MS) and Prussian blue staining showed that the RSGADNPs were internalized into the NIH 3T3 cells compared to the RGADNPs. These results suggest that RSGADNP may find ever-growing applications in biological labels and detection or contrast agents in life science and medical diagnostics.
( Zhi-ying Cai ),( Yi-xian Liu ),( Yu-ping Shi ),( Li-ming Dai ),( Lan-lan Li ),( Hong-jun Mu ),( Mei-lin Lv ),( Xiao-yong Liu ) 한국균학회 2019 Mycobiology Vol.47 No.1
A new species of Alternaria causing leaf spots on the rubber tree (Hevea brasiliensis) in Yunnan, China, was isolated, examined, and illustrated. Morphologically, it belongs to the section Porri of Alternaria, which produces relatively large conidia and a simple or branched, filamentous long beak. It is, however, characterized by conidiophores gradually enlarging near the apex into a clavate conidiogenous cell and long ellipsoid to obclavate, smoothwalled conidia with a long filamentous beak. Molecular phylogenetic analyses based on ITS rDNA, GAPDH, and TEF1-alpha sequences demonstrate that the phytopathogen falls in the clade of the section Porri, being most closely related to A. sidae, A. sennae, A. deseriticola, A. cyamopsidis, A. rostellata, A. nitrimali, A. crassa, and A. thunbergiae.
Zhi-Jiang Cai,Qin Zhang,Xian-You Song 대한금속·재료학회 2016 ELECTRONIC MATERIALS LETTERS Vol.12 No.6
Polyindole/carbon nanotubes (PIN/CNTs) composite was prepared byan in-situ chemical oxidative polymerization of indole monomer withCNTs using ammonium persulfate as oxidant. The obtained compositematerial was characterized by SEM, TEM, FT-IR, Raman spectroscopy,XPS, XRD and BET surface areas measurements. It was found that theCNTs were incorporated into the PIN matrix and nanoporous structurewas formed. Spectroscopy results showed that interfacial interactionbonds might be formed between the polyindole chains and CNTs duringthe in-situ polymerization. PIN/CNTs composite was evaluated byelectrochemical impedance spectroscopy, cyclic voltammetry andcharge/discharge tests to determine electrode performances in relation tosupercapacitors properties in both aqueous and non-aqueous system. Amaximum specific capacitance and specific volumetric capacitance of555.6 F/g and 222.2 F/cm3 can be achieved at 0.5 A/g in non-aqueoussystem. It also displayed good rate performance and cycling stability. The specific capacitance retention is over 60% at 10 A/g and 91.3%after 5000 cycles at 2 A/g, respectively. These characteristics point to itspromising applications in the electrode material for supercapacitors.
ValueRank: Keyword Search of Object Summaries Considering Values
( Cai Zhi ),( Lan Xu ),( Su Xing ),( Lang Kun ),( Cao Yang ) 한국인터넷정보학회 2019 KSII Transactions on Internet and Information Syst Vol.13 No.12
The Relational ranking method applies authority-based ranking in relational dataset that can be modeled as graphs considering also their tuples’ values. Authority directions from tuples that contain the given keywords and transfer to their corresponding neighboring nodes in accordance with their values and semantic connections. From our previous work, ObjectRank extends to ValueRank that also takes into account the value of tuples in authority transfer flows. In a maked difference from ObjectRank, which only considers authority flows through relationships, it is only valid in the bibliographic databases e.g. DBLP dataset, ValueRank facilitates the estimation of importance for any databases, e.g. trading databases, etc. A relational keyword search paradigm Object Summary (denote as OS) is proposed recently, given a set of keywords, a group of Object Summaries as its query result. An OS is a multilevel-tree data structure, in which node (namely the tuple with keywords) is OS’s root node, and the surrounding nodes are the summary of all data on the graph. But, some of these trees have a very large in total number of tuples, size-l OSs are the OS snippets, have also been investigated using ValueRank.We evaluated the real bibliographical dataset and Microsoft business databases to verify of our proposed approach.
A Power and Performance Management Simulation Platform for Web Application Server Cluster
Zhi Xiong,Zhongliang Xue,Weihong Cai,Lingru Cai,Juan Yang 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.12
Web application server cluster has been widely used to improve the performance of web application servers. Because web load is highly variable, we need to dynamically manage cluster’s deployment so as to reduce power consumption and meanwhile satisfy load performance demand. To facilitate researchers to evaluate a management strategy or choose key parameters for it, we propose a CloudSim-based simulation platform in this paper. It can simulate different cluster deployment algorithm, request scheduling algorithm and load feature, where cluster’s deployment includes the on/off state, CPU frequency and request scheduling parameter(s) of each server. By the aid of HookTimer component, the platform supports periodical and conditional deployment trigger modes, and can calculate some common performance indicators. The usage of interface, dynamic proxy technique and XML configuration file make the platform have good extensibility and configurability. In addition, a request-number-triggered management strategy is proposed and simulated by the platform. The simulation results demonstrate the feasibility of the platform.
Fabrication of Biodegradable Polyester Nanocomposites by Electrospinning for Tissue Engineering
Xing, Zhi-Cai,Han, Seung-Jin,Shin, Yong-Suk,Kang, Inn-Kyu Hindawi Publishing Corporation 2011 Journal of nanomaterials Vol.2011 No.-
<P>Recently, nanocomposites have emerged as an efficient strategy to upgrade the structural and functional properties of synthetic polymers. Polyesters have attracted wide attention because of their biodegradability and biocompatibility. A logic consequence has been the introduction of natural extracellular matrix (ECM) molecules, organic or inorganic nanostructures to biodegradable polymers to produce nanocomposites with enhanced properties. Consequently, the improvement of the interfacial adhesion between biodegradable polymers and natural ECM molecules or nanostructures has become the key technique in the fabrication of nanocomposites. Electrospinning has been employed extensively in the design and development of tissue engineering scaffolds to generate nanofibrous substrates of synthetic biodegradable polymers and to simulate the cellular microenvironment. In this paper, several types of biodegradable polyester nanocomposites were prepared by electrospinning, with the aim of being used as tissue engineering scaffolds. The combination of biodegradable nanofibrous polymers and natural ECM molecules or nanostructures opens new paradigms for tissue engineering applications.</P>
Xing, Zhi-Cai,Meng, Wan,Yuan, Jiang,Moon, Sungmo,Jeong, Yongsoo,Kang, Inn-Kyu Hindawi Limited 2012 Journal of nanomaterials Vol.2012 No.-
<P>Flavonoids, such as quercetin, have been reported to exhibit a wide range of biological activities related to their antioxidant capacity. The aim of this study was to investigate the protective effect of quercetin on cell adhesion, and the viability and proliferation of KB epithelial cells. Quercetin- (1, 5 wt%)-containing poly (l-lactide-co-glycolide) (PLGA) nanofibrous scaffolds (PLGA/Q 1, PLGA/Q 5) were prepared by electrospinning technique and their antibacterial properties were examined. Two types of bacteria strains,<I>Staphylococcus aureus</I>(SA) and<I>Klebsiella pneumoniae</I>(KP), were used to evaluate the antibacterial properties of the scaffolds. The results showed that the quercetin-containing PLGA nanofibrous scaffolds exhibited significant antibacterial effects against the two bacterial strains. KB epithelial cells were also used to evaluate the cytocompatibility of the scaffolds. From the results, it was found that the PLGA nanofibrous scaffolds with 1 wt% of quercetin had good cell compatibility. It is considered that the PLGA nanofibrous scaffolds with 1 wt% quercetin have potential to be used in tissue engineering.</P>
Xing, Zhi-Cai,Chae, Won-Pyo,Huh, Man-Woo,Park, Lee-Soon,Park, Soo-Young,Kwak, Giseop,Yoon, Keun-Byoung,Kang, Inn-Kyu American Scientific Publishers 2011 Journal of Nanoscience and Nanotechnology Vol.11 No.1
<P>Electrospinning has recently emerged as a leading technique for the formation of nanofibrous structures made of organic and inorganic components. In this study, nanofibrous scaffolds were prepared by electrospining a bend solution of poly(L-lactide-co-glycolide) (PLGA) and silver nanoparticles in 1,1,1,3,3,3,-hexafluoro-2-propanol (HFIP). The resulting fibers ranged from 420 to 590 nm in diameter. To evaluate the possibility of using silver-containing PLGA as a tissue engineering scaffold, experiments on cell viability and antibacterial activity were carried out. As a result, PLGA nanofibrous scaffolds having silver nanoparticles of more than 0.5 wt% showed antibacterial effect against Staphylococcus aureus and Klebsiella pneumonia. Furthermore, silver-containing PLGA nanofibrous scaffolds showed viability, indicating their possible application in the field of tissue engineering.</P>