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RISS 인기검색어
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- 저자 : ( Xinqi Xu ) (검색결과 5 건)
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Molecular discrimination of Panax ginseng cultivar K-1 using pathogenesis-related protein 5 gene
Wang, Hongtao,Xu, Fengjiao,Wang, Xinqi,Kwon, Woo-Saeng,Yang, Deok-Chun The Korean Society of Ginseng 2019 Journal of Ginseng Research Vol.43 No.3
Background: The mixed-cultivation of different Panax ginseng cultivars can cause adverse effects on stability of yield and quality. K-1 is a superior cultivar with good root shape and stronger disease resistance. DNA markers mined from functional genes are clearly desirable for K-1, as they may associate with major traits and can be used for marker-assisted selection to maintain the high quality of Korean ginseng. Methods: Five genes encoding pathogenesis-related (PR) proteins of P. ginseng were amplified and compared for polymorphism mining. Primary, secondary, and tertiary structures of PR5 protein were analyzed by ExPASy-ProtParam, PSSpred, and I-TASSER methods, respectively. A coding single nucleotide polymorphism (SNP)-based specific primer was designed for K-1 by introducing a destabilizing mismatch within the 3' end. Allele-specific polymerase chain reaction (PCR) and real-time allele-specific PCR assays were conducted for molecular discrimination of K-1 from other cultivars and landraces. Results: A coding SNP leading to the modification of amino acid residue from aspartic acid to asparagine was exploited in PR5 gene of K-1 cultivar. Bioinformatics analysis showed that the modification of amino acid residue changed the secondary and tertiary structures of the PR5 protein. Primer KSR was designed for specific discrimination of K-1 from other ginseng cultivars and landraces. The developed real-time allele-specific PCR assay enabled easier automation and accurate genotyping of K-1 from a large number of ginseng samples. Conclusion: The SNP marker and the developed real-time allele-specific PCR assay will be useful not only for marker-assisted selection of K-1 cultivar but also for quality control in breeding and seed programs of P. ginseng.
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Molecular discrimination of Panax ginseng cultivar K-1 using pathogenesis-related protein 5 gene
Hongtao Wang,Fengjiao Xu,Xinqi Wang,권우생,Deok Chun Yang 고려인삼학회 2019 Journal of Ginseng Research Vol.43 No.3
Background: The mixed-cultivation of different Panax ginseng cultivars can cause adverse effects onstability of yield and quality. K-1 is a superior cultivar with good root shape and stronger diseaseresistance. DNA markers mined from functional genes are clearly desirable for K-1, as they may associatewith major traits and can be used for marker-assisted selection to maintain the high quality of Koreanginseng. Methods: Five genes encoding pathogenesis-related (PR) proteins of P. ginseng were amplified andcompared for polymorphism mining. Primary, secondary, and tertiary structures of PR5 protein wereanalyzed by ExPASy-ProtParam, PSSpred, and I-TASSER methods, respectively. A coding single nucleotidepolymorphism (SNP)ebased specific primer was designed for K-1 by introducing a destabilizingmismatch within the 30 end. Allele-specific polymerase chain reaction (PCR) and real-time allele-specificPCR assays were conducted for molecular discrimination of K-1 from other cultivars and landraces. Results: A coding SNP leading to the modification of amino acid residue from aspartic acid to asparaginewas exploited in PR5 gene of K-1 cultivar. Bioinformatics analysis showed that the modification of aminoacid residue changed the secondary and tertiary structures of the PR5 protein. Primer KSR was designedfor specific discrimination of K-1 from other ginseng cultivars and landraces. The developed real-timeallele-specific PCR assay enabled easier automation and accurate genotyping of K-1 from a large numberof ginseng samples. Conclusion: The SNP marker and the developed real-time allele-specific PCR assay will be useful not onlyfor marker-assisted selection of K-1 cultivar but also for quality control in breeding and seed programs ofP. ginseng.
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( Min Shen ),( Xin Ju ),( Xinqi Xu ),( Xuemei Yao ),( Liangzhi Li ),( Jiajia Chen ),( Cuiying Hu ),( Jiaolong Fu ),( Lishi Yan ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.7
In this study, we attempted to find new and efficient microbial enzymes for producing rare sugars. A ribose-5-phosphate isomerase B (OsRpiB) was cloned, overexpressed, and preliminarily purified successfully from a newly screened Ochrobactrum sp. CSL1, which could catalyze the isomerization reaction of rare sugars. A study of its substrate specificity showed that the cloned isomerase (OsRpiB) could effectively catalyze the conversion of L-rhamnose to L-rhamnulose, which was unconventional for RpiB. The optimal reaction conditions (50oC, pH 8.0, and 1 mM Ca<sup>2+</sup>) were obtained to maximize the potential of OsRpiB in preparing L-rhamnulose. The catalytic properties of OsRpiB, including K<sub>m</sub>, K<sub>cat</sub>, and catalytic efficiency (K<sub>cat</sub>/K<sub>m</sub>), were determined as 43.47 mM, 129.4 sec<sup>-1</sup>, and 2.98 mM/sec. The highest conversion rate of L-rhamnose under the optimized conditions by OsRpiB could reach 26% after 4.5 h. To the best of our knowledge, this is the first successful attempt of the novel biotransformation of L-rhamnose to L-rhamnulose by OsRpiB biocatalysis.
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Zou Yutong,Wang Danchen,Cheng Xinqi,Ma Chaochao,Lin Songbai,Hu Yingying,Yu Songlin,Xia Liangyu,Li Honglei,Yin Yicong,Liu Huaicheng,Zhang Dianxi,Zhang Kui,Lian Xiaolan,Xu Tengda,Qiu Ling 대한진단검사의학회 2021 Annals of Laboratory Medicine Vol.41 No.1
Background: Thyroid diseases are highly prevalent worldwide, but their diagnosis remains a challenge. We established reference intervals (RIs) for thyroid-associated hormones and evaluated the prevalence of thyroid diseases in China. Methods: After excluding outliers based on the results of ultrasound screening, thyroid antibody tests, and the Tukey method, the medical records of 20,303 euthyroid adults, who visited the Department of Health Care at Peking Union Medical College Hospital from January 2014 to December 2018, were analyzed. Thyroid-associated hormones were measured by the Siemens Advia Centaur XP analyzer. The RIs for thyroid-associated hormones were calculated according to the CLSI C28-A3 guidelines, and were compared with the RIs provided by Siemens. The prevalence of thyroid diseases over the five years was evaluated and compared using the chi-square test. Results: The RIs for thyroid stimulating hormone (TSH), free thyroxine (FT4), free triiodothyronine (FT3), total thyroxine (TT4), and total triiodothyronine (TT3) were 0.71–4.92 mIU/L, 12.2–20.1 pmol/L, 3.9–6.0 pmol/L, 65.6–135.1 nmol/L, and 1.2–2.2 nmol/L, respectively. The RIs of all hormones except TT4 differed significantly between males and females. The RIs of TSH increased with increasing age. The prevalence of overt hypothyroidism, overt hyperthyroidism, subclinical hypothyroidism, and subclinical hyperthyroidism was 0.5% and 0.8%, 0.2% and 0.6%, 3.8% and 6.1%, and 3.3% and 4.7% in males and females, respectively, which differed from those provided by Siemens. Conclusions: Sex-specific RIs were established for thyroid-associated hormones, and the prevalence of thyroid diseases was determined in the Chinese population.
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A New BODIPY Derivative Bearing Piperazine Group
Xin Qi,김숙경,Eun Jin Jun,Li Xu,김성진,윤주영 대한화학회 2007 Bulletin of the Korean Chemical Society Vol.28 No.12
A new BODIPY derivative bearing piperazine group was synthesized and its fluorescent changes towards metal ions as well as pH are studied. The title compound displayed a moderate selectivity for Hg2+ among the metal ions examined.
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