CK2 phosphorylates AP-2α and increases its transcriptional activity

( Kai Qun Ren ),( Shuang Lin Xiang ),( Fang Li He ),( Wen Feng Zhang ),( Xiao Feng Ding ),( Yan Yang Wu ),( Li Ping Yang ),( Jian Lin Zhou ),( Xiang Gao ),( Jian Zhang ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.7

Transcription factor AP-2α involves in the process of mammalian embryonic development and tumorigenesis. Many studies have shown that AP-2α functions in association with other interacting proteins. In a two-hybrid screening, the regulatory subunit β of protein casein kinase 2 (CK2β) was identified as an interacting protein of AP-2α; we confirmed this interaction using in-vitro GST pull-down and in-vivo co-immunoprecipitation assays; in an endogenous co-immunoprecipitation experiment, we further found the catalytic subunit α of protein casein kinase 2 (CK2α) also exists in the complex. Phosphorylation analysis revealed that AP-2α was phosphorylated by CK2 kinase majorly at the site of Ser429, and such phosphorylation could be blocked by CK2 specific inhibitor 4,5,6,7-tetrabromobenzotriazole (TBB) in a dose-dependent manner. Luciferase assays demonstrated that both CK2α and CK2β enhanced the transcription activity of AP-2α; moreover, CK2β increased the stability of AP-2α. Our data suggest a novel cellular function of CK-2 as a transcriptional co-activator of AP-2α.

Containment Control of Continuous-time Multi-agent Systems with General Linear Dynamics under Time-varying Communication TopologiesTopologies

Zhe Yang,Xiao-wu Mu,Kai Liu 제어·로봇·시스템학회 2017 International Journal of Control, Automation, and Vol.15 No.1

This paper devotes itself to the containment tracking problem of general linear high-order multiagentsystems (MASs) under time-varying communications. For general linear MASs with time-varying weightunbalanceddigraph, the containment control problem is difficult and challenging, because the Lyapunov methodis not an effective approach in this case. Under the assumption that the graph topology uniformly and jointlyhas a directed spanning forest, we show that when the exponentially unstable mode associated with each agent’sself-dynamics is weak enough, the followers can asymptotically tend to the dynamical convex hull spanned bythe leaders, i.e. the containment can be achieved. Moreover, the least convergence rate is explicitly specified. Simulations are also provided to demonstrate the effectiveness of our result.

Genetic Epidemiological Analysis of Esophageal Cancer in High-incidence Areas of China

Wang, Kai-Juan,Yang, Jun-Xia,Shi, Jia-Chen,Deng, Song-Yuan,Cao, Xiao-Qin,Song, Chun-Hua,Wang, Peng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

Genetic epidemiological studies have shown that genetic susceptibility to esophageal cancer (EC) is an important cause of its high incidence within families in some areas of China. The purpose of this study was to obtain evidence of a genetic basis of EC in Xin-an and Xin-xiang counties in China. Familial aggregation and complex segregation analyses were performed of 79 EC families in these counties. The heritability of EC was examined using Falconer's method and complex segregation analysis was conducted with the SEGREG program in Statistical Analysis for Genetic Epidemiology (SAGE version 5.3.1). The results showed that the distribution of EC in families did not fit well into a binomial distribution. The heritability of EC among first-degree and second-degree relatives was $67.0{\pm}7.31%$ and $43.1%{\pm}9.80%$, respectively, and the summing up powered heritability was $53.2{\pm}6.74%$. The segregation ratio was 0.045. Complex segregation analysis showed that the genetic model of EC was additive. The current results provide evidence for an inherited propensity to EC in certain high-risk groups in China, and support efforts to identify the genes that confer susceptibility to this disease.

Research on the influence of union-pay M-payment quality and brand personality on user viscosity

Zi-Yang Liu,Jun-Lin Wang,Jiayu Liu,Xiao-yin Liu,Kai Liao 한국컴퓨터정보학회 2020 韓國컴퓨터情報學會論文誌 Vol.25 No.4

이 연구의 목적은 서비스 품질 및 브랜드 특성의 관점에서 Union-Pay 모바일 결제가 사용자 점도에 미치는 영향을 탐색하는 것이다. 앞으로 중국Union-Pay가 모바일결제 시장에서 안정적이고 충실한 사용자 그룹을 확보하는 것이 의미가 있다. 이 연구는 SPSS22.0 및 AMOS 통계 분석 도구를 사용하여 경험적 연구를 수행을 했다. 본 연구는 모바일 서비스 품질을 사용하고 브랜드 성격은 독립 변수이고, 사용자의 점도는 종속 변수이며, China Union-Pay 모바일 결제와 관련하여 사용자의 점도에 미치는 영향을 연구하였다. 연구 분석결과에 따르면, 서비스 품질이 사용자가 인식 한 가치에 유의 한 영향을 미친다는 결과 나타났다. 서비스 품질은 사용자 점도에 유의 한 양의 관계를 갖다. 인식 된 값은 사용자 점도에 유의한 양의 효과를 나타났다. 사용자에 대한 브랜드 특성이 사용자 점도에 유의한 양의 효과를 나타났다. 브랜드 성격은 사용자가 인식 한 가치에 유의 한 양의 관계 영향을 미친다. 이 연구를 통해 모바일 결제 기업의 산업 발전에 대한 제안을 했고 모바일 결제발전에 대한 더 나은 의견을 제시 하였다. The purpose of this study is to explore the impact of Union-Pay mobile payments on user viscosity from the perspective of service quality and brand personality. In the future, it is meaningful for CUP to obtain a stable and loyal user group in China’s mobile payment market. This study uses SPSS22.0 and AMOS statistical analysis tools to conduct empirical research. In this case, this study uses mobile service quality and The brand personality is the independent variable, and the user’s viscosity is the dependent variable, which studies the impact on the user’s viscosity in the context of China Union-Pay mobile payment. the study. According to the analysis results, the research goal: service quality has a significant positive correlation effect on user perceived value; service quality has a significant positive correlation effect on user viscosity; perceived value has significant positive correlation effect on user viscosity; brand personality to user Viscosity has a significant positive correlation effect; brand personality has a significant positive correlation effect on user perceived value. Through this research, we can make Suggestions on the industrial development of mobile payment enterprises and provide better opinions on the development of mobile payment.

EFFECT OF HEAVY-DUTY DIESEL ENGINE OPERATING PARAMETERS ON PARTICLE NUMBER AND SIZE DISTRIBUTION AT LOW SPEED CONDITION

Bin Yang Wu,Qiang Zhang,Shun Kai Zhang,Xiao Kun Nie,Yu Han Li,Wanhua Su 한국자동차공학회 2018 International journal of automotive technology Vol.19 No.4

Experiments and simulations were used to investigate the effect of a range of engine operating parameters and fuel characteristics on the particle size and particle number (PN) concentration at low speed and idle speed condition. The occurrence, size, and concentration of particles were tested against a range of parameters including start of injection (SOI), common rail pressure, exhaust gas recirculation (EGR) ratio and load. The results showed that the homogeneity of the mixture had the greatest impact on particle size and number concentration. The performance of particle is different at different levels of load. The particle were of nucleation mode at idle condition, and the cold idle particles had a slightly larger diameter than those produced at hot idle. By using the diesel and under high load, at EGR ratios of less than 20 %, most particles were of nucleation mode. At EGR ratios exceeding 20 %, nucleation-mode particles were gradually replaced by accumulation-mode particles. At EGR ratios above 30 %, most particles were of the accumulation mode. Under the same load, gasoline compression ignition produced particles of smaller size and reduced particulate mass (PM). The use of gasoline extended ignition delay, as the high volatility and octane number of the fuel improved the homogeneity of the mixture. Finally, a linear relationship was found between PM and PN. The relative contribution of the different factors to the formation of nucleationor accumulation-mode particles was investigated.

Market Led Industrialization and Globalization

( Jeffrey Sachs ),( Xiao Kai Yang ) 세종대학교 경제통합연구소 2002 Journal of Economic Integration Vol.17 No.2

The paper introduces asymmetric production conditions between firms and asymmetric transaction conditions between countries into the Murphy-Shleifer-Vishny model of industrialization. It explores a general equilibrium mechanism that generates circular causation loop that each firm`s profitability and its decision of involvement in a network of industrial linkages is determined by the size of the network, while the network size is in turn determined by all firms` decisions of participation. It shows that the very function of the market is to network relevant self-interested decision-makers and to utilize the network effects of industrialization, though this function is not perfect. Hence, market led industrialization will gradually spread until the whole world economy is integrated in a single network of trade and industrial linkages as trading efficiency is improved. This paper devises a new approach to specifying zero profit condition for a marginal modern firm, while keeping original feedback loop between positive profit and the extent of the market of the MSV model. Hence, this new method and the trade off between economies of scale and transaction costs can be used to endogenize the number of modern sectors, thereby increasing applicability of this type of models.

The effect of biogas slurry application on biomass production and the silage quality of corn

Hua Sun,Kai Shi,Hairong Ding,Chenglong Ding,Zhiqing Yang,Chen An,Chongfu Jin,Beiyi Liu,Zhaoxin Zhong,Xia Xiao,Fuyin Hou Asian Australasian Association of Animal Productio 2023 Animal Bioscience Vol.36 No.12

Objective: The objective of this study was to evaluate the effect of biogas slurry application on biomass production and the silage quality of corn. Methods: A field experiment was conducted in which corn was grown using different biogas slurry application rates. The effect of 25% to 500% biogas slurry nitrogen replacement (T1 to T14) on the yield and quality indices of corn were studied by field plot experiments. Results: The results revealed that biogas slurry application improved the stem diameter and relative feed value of corn silage in treatments T13 and T11. Moreover, the fermentation quality of corn silage was improved due to an increase in lactic acid content; in comparison with the chemical synthetic fertilizer (CF) group. The crude protein contents of corn silage had no obvious change with increasing biogas slurry application. However, the forage quality index of acid detergent fiber was decreased (p<0.05) in the T11 group compared with the CF group. In addition, higher (p<0.05) 30 h in vitro dry matter digestibility and 30 h in vitro neutral detergent fiber digestibility were observed in the T11 and T13 groups than in the CF group. Conclusion: Based on these results, it was concluded that the optimum biogas slurry application rate for corn was approximately 350% to 450% biogas slurry nitrogen replacement under the present experimental conditions.

Removal of the Glycosylation of Prion Protein Provokes Apoptosis in SF126

( Lan Chen ),( Yang Yang ),( Jun Han ),( Bao Yun Zhang ),( Lin Zhao ),( Kai Nie ),( Xiao Fan Wang ),( Feng Li ),( Chen Gao ),( Xiao Ping Dong ),( Cai Min Xu ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.5

Non-thermal plasma enhances rice seed germination, seedling development, and root growth under low-temperature stress

Bian Jing-Yang,Guo Xiao-Yu,Lee Dong Hun,Sun Xing-Rong,Liu Lin-Shuai,Shao Kai,Liu Kai,Sun Hu-Nan,Kwon Taeho 한국응용생명화학회 2024 Applied Biological Chemistry (Appl Biol Chem) Vol.67 No.-

Recently, non-thermal plasma (NTP) technologies have found widespread application across diverse fields, including plant growth, medical science, and biological and environmental research. Rice (Oryza sativa L.) is exceptionally sensitive to temperature changes. Notably, low-temperature stress primarily affects the germination and reproductive stages of rice, often leading to reduced crop yield. This study aimed to identify optimal conditions for enhancing rice seed germination and seedling growth under low temperatures using NTP technology. Our research indicated that NTP treatment at 15.0 kV for 30 s optimally promotes rice seed germination and growth under low-temperature stress. Furthermore, NTP treatment increases the activity and expression of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD), under low-temperature conditions. Moreover, it downregulates the expression of β-ketoacyl-[acyl carrier protein] synthase I (KASI) and cis-epoxy carotenoid dioxygenase 3 (NCED3) and upregulates the expression of alternative oxidase (AOX1B), BREVIS RADIX-like homologous gene (BRXL2), WRKY transcription factor 29 (WRKY29), and EREBP transcription factor 2 (EREBP2) in roots after tandem 7 days lowtemperature (16 ℃) and 7 days room-temperature (28 ℃) treatments. Transcriptomic analysis revealed the involvement of various key genes in phosphotransferase activity, phosphate-containing compound metabolic processes, and defense responses. These analyses provide comprehensive information on gene expression at the transcriptional level, offering new insights for a deeper understanding of candidate genes required for root growth in rice.

Removal of the Glycosylation of Prion Protein Provokes Apoptosis in SF126

Chen, Lan,Yang, Yang,Han, Jun,Zhang, Bao-Yun,Zhao, Lin,Nie, Kai,Wang, Xiao-Fan,Li, Feng,Gao, Chen,Dong, Xiao-Ping,Xu, Cai-Min Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.5

Although the function of cellular prion protein (PrP$^C$) and the pathogenesis of prion diseases have been widely described, the mechanisms are not fully clarified. In this study, increases of the portion of non-glycosylated prion protein deposited in the hamster brains infected with scrapie strain 263K were described. To elucidate the pathological role of glycosylation profile of PrP, wild type human PrP (HuPrP) and two genetic engineering generated non-glycosylated PrP mutants (N181Q/N197Q and T183A/T199A) were transiently expressed in human astrocytoma cell line SF126. The results revealed that expressions of non-glycosylated PrP induced significantly more apoptosis cells than that of wild type PrP. It illustrated that Bcl-2 proteins might be involved in the apoptosis pathway of non-glycosylated PrPs. Our data highlights that removal of glycosylation of prion protein provokes cells apoptosis.