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Jung, Won-Kyo,Jo, Hee-Yeon,Qian, Zhong-Ji,Jeong, Young-Ju,Park, Sae-Gwang,Choi, Il-Whan,Kim, Se-Kwon Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.5
A novel inhibitory protein against blood coagulation factor Va (FVa) was purified from muscle protein of granulated ark (Tegillarca granosa, order Arcoida, marine bivalvia) by consecutive FPLC method using anion exchange and gel permeation chromatography. In the results of ESI-QTOF tandem mass analysis and database research, it was revealed that the purified T. granosa anticoagulant protein (TGAP) has 7.7 kDa of molecular mass and its partial sequence, HTHLQRAPHPNALGYHGK, has a high identity (64%) with serine/threonine kinase derived from Rhodopirellula baltica (order Planctomycetales, marine bacteria). TGAP could potently prolong thrombin time (TT), corresponding to inhibition of thrombin (FIIa) formation. Specific factor inhibitory assay showed that TGAP inhibits FVa among the major components of prothrombinase complex. In vitro assay for direct-binding affinity using surface plasmon resonance (SPR) spectrometer indicated that TGAP could be directly bound with FVa. In addition, the binding affinity of FVa to FII was decreased by addition of TGAP in dose-dependant manner ($IC_{50}$ value = 77.9 nM). These results illustrated that TGAP might interact with a heavy chain of FVa ($FVa_H$) bound to FII in prothrombin complex. The present study elucidated that non-cytotoxic T. granosa anticoagulant protein (TGAP) bound to FVa can prolong blood coagulation time by inhibiting conversion of FII to FIIa in blood coagulation cascade. In addition, TGAP did not significantly (P < 0.05) show fibrinolytic activity and cytotoxicity on venous endothelial cell line (ECV 304).
Antiproliferative action of metformin in endometrial stromal cell and endometrial cancer cell
( Jung Won Yoon ),( Ji Ann Jung ),( Young Eun Jun ),( Si Hyun Cho ),( Young Sik Choi ),( Byung Seok Lee ),( Seok Kyo Seo ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.98 No.-
The aim of this study was to investigate the antitumor effects of metformin in inflammatory response and proliferation of endometrial stromal cells (ESCs) and endometrial cancer cell lines. Normal human ESCs were obtained from normal fertile women, who were undergoing hysterectomy for fibroids. ECC-1 and Ishikawa cells were used as endometrial cancer cell lines. The antiproliferative effects of metformin were assessed in endometrial cancer cell lines and normal human ESCs at various concentrations of metformin. Proliferation and apoptosis was assessed by flow cytometry. Metformin decreased IL-1β-induced IL-8 production and inhibited growth of ESCs in a dose-dependent manner. Metformin also exerts antiproliferative effects on both ECC-1 and Ishikawa cell in a dose-dependent manner. High dose of metformin induced apoptosis in both ECC-1 and Ishikawa cells. Our findings suggest that metformin is a potent inhibitor of cell proliferation in ESCs and endometrial cancer cell lines. Further researchs are needed to investigate metformin as a strategy for endometrial cancer prevention in patients with polycystic ovary syndrome.