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Transcriptome analysis of a tropical medicinal plant, Pistacia weinmannifolia
Mi Kyung Choi(Mi Kyung Choi),Bimpe Suliyat Azeez(Bimpe Suliyat Azeez ),Sang Woo Lee(Sang Woo Lee),Wan Yi Li(Wan Yi Li),Sangho Choi(Sangho Choi),Ik-Young Choi(Ik-Young Choi),Ki-Young Choi(Ki-Young (사)한국식물생명공학회 2024 JOURNAL OF PLANT BIOTECHNOLOGY Vol.51 No.1
Regioselective Synthesis of 1,3‐ and 1,2‐Tocopheryl Glyceride Ethers of Carboxylic Acids
Choi, Bo Seul,Choi, Jeongae,Bak, Seonyoung,Koo, Sangho WILEY‐VCH Verlag 2015 European journal of organic chemistry Vol.2015 No.3
<P><B>Abstract</B></P><P>A regioselective synthetic method with which to access 1,3‐tocopheryl glyceride ethers <B>2</B> of diverse carboxylic acids was developed through Bu<SUB>4</SUB>NBr‐catalyzed ring‐opening of tocopheryl glycidyl ether <B>1</B>. Steglich esterification of the 1,3‐tocopheryl acetyl‐glyceride ether <B>2a</B> with various carboxylic acids, followed by selective deprotection of 3‐acetate by LiEt<SUB>3</SUB>BH reduction at –78 °C constitutes a regioselective synthetic method of 1,2‐tocopheryl glyceride ethers <B>4</B>. An example of the differing self‐assembled behavior of phosphorylcholine derivatives <B>6c</B> and <B>7c</B> of 1,3‐ and 1,2‐tocopheryl stearoyl‐glyceride ethers in aqueous solution is also described.</P>
Choi, Sangho,Jeong, Hang Jin,Hwang, Kyu-Jam,Gill, Byoungchul,Ju, Young Ran,Lee, Yeong Seon,Lee, Jeongmin Allen Press, etc 2017 The American journal of tropical medicine and hygi Vol.97 No.1
<P><B>Abstract.</B></P><P>We investigated the 47-kDa outer membrane protein (OMP), which is a periplasmic serine protease and an antigenic major surface protein of <I>Orientia tsutsugamushi</I>, as a vaccine candidate. We developed a conventional subunit vaccine expressing recombinant 47-kDa OMP (rec47) and a DNA vaccine (p47). In mouse immunization experiments, intranasal immunization with rec47 alone or with rec47 plus heat-labile enterotoxin B subunit from <I>Escherichia coli</I> or plus cholera toxin (CT) as adjuvants induced a higher amount of rec47-specific antibodies than intramuscular immunization with p47 alone or with p47 plus pBOOST2-samIRF7/3 (pB) as adjuvant. Moreover, the combination of rec47 and CT induced a strong cellular immune response to 47-kDa OMP, as demonstrated by a spleen cell proliferation assay, and also induced Th1- and Th2-type cytokine production, as demonstrated by a cytokine enzyme-linked immunosorbent assay. Intranasal immunization with rec47 plus CT was the most effective method for the induction of humoral and cell-mediated immune responses. Furthermore, relatively strong protection against homologous <I>O. tsutsugamushi</I> strain Boryong challenge was observed in mice immunized with rec47 plus CT. Therefore, 47-kDa OMP is an attractive candidate for developing a prophylactic vaccine against scrub typhus by <I>O. tsutsugamushi</I> infection.</P>
Choi, Sangho,Kim, Sung Jin Pergamon Press 2017 International journal of heat and mass transfer Vol. No.
<P><B>Abstract</B></P> <P>The effect of initial wall cooling from ambient temperature to cryogenic temperature on heat and mass transfer at the cryogenic surface under natural convection was experimentally and numerically investigated. The experimental study showed that the initial wall cooling had a strong effect on heat and mass transfer at the cryogenic surface. The frost under initial wall cooling grew considerably thicker than the case without initial wall cooling. The maximum heat flux under initial wall cooling was 40% of that without initial wall cooling, and the minimum heat flux under the initial wall cooling was 52% of that without initial wall cooling. In addition, a numerical model for the frost formation accounting for initial wall cooling was proposed. The proposed numerical model could explain the heat and mass transfer at the cryogenic surface during the cooling process as well as the filling and holdup process. In order to validate the proposed numerical model, experiments were performed under various ambient air temperature and relative humidity conditions: 10°C≤Ta≤30°C and 30%≤RH≤90%. The maximum and minimum heat flux from the numerical model showed good agreement with experimental data within 10% and 25% error, respectively. The final frost thickness from the numerical model showed good agreement with experimental data within 13% error except for one case where mass transfer was reduced due to fog formation near the cryogenic surface. Therefore, the numerical model will be useful for estimating the heat flux in an uninsulated cryogenic system, such as a rocket oxygen tank.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The effect of initial cooling on frost formation at the cryogenic surface is presented. </LI> <LI> The frost under an initial cooling grows thicker than the case without cooling. </LI> <LI> The frost formation under an initial cooling severely reduces wall heat flux. </LI> <LI> The effect of ambient air conditions on heat and mass transfer is presented. </LI> <LI> A numerical model of frost formation accounting for an initial cooling is proposed. </LI> </UL> </P>
Sequence variations of the bovine prion protein gene (PRNP) in native Korean Hanwoo cattle
Sangho Choi,우희종,이정민 대한수의학회 2012 JOURNAL OF VETERINARY SCIENCE Vol.13 No.2
Bovine spongiform encephalopathy (BSE) is one of the fatal neurodegenerative diseases known as transmissible spongiform encephalopathies (TSEs) caused by infectious prion proteins. Genetic variations correlated with susceptibility or resistance to TSE in humans and sheep have not been reported for bovine strains including those from Holstein,Jersey, and Japanese Black cattle. Here, we investigated bovine prion protein gene (PRNP) variations in Hanwoo cattle [Bos (B.) taurus coreanae], a native breed in Korea. We identified mutations and polymorphisms in the coding region of PRNP, determined their frequency, and evaluated their significance. We identified four synonymous polymorphisms and two non-synonymous mutations in PRNP, but found no novel polymorphisms. The sequence and number of octapeptide repeats were completely conserved, and the haplotype frequency of the coding region was similar to that of other B. taurus strains. When we examined the 23-bp and 12-bp insertion/deletion (indel) polymorphisms in the non-coding region of PRNP, Hanwoo cattle had a lower deletion allele and 23-bp del/12-bp del haplotype frequency than healthy and BSE-affected animals of other strains. Thus, Hanwoo are seemingly less susceptible to BSE than other strains due to the 23-bp and 12-bp indel polymorphisms.
( Sangho Choi ),( Hang Jin Jeong ),( Byoungchul Gill ),( Yeong Seon Lee ),( Jeongmin Lee ) 대한인수공통전염병학회 2016 창립총회 및 학술대회 초록집 Vol.2016 No.1
Background: Scrub typhus is an acute, febrile, and potentially fatal mite-borne disease that is caused by infection with Orientia tsutsugamushi. In Korea, the incidence has increased due to environmental changes and increased outdoor activity leading to frequent exposure to chigger mite vectors. Although the infection is treatable with doxycycline and azithromycin, an effective prophylactic vaccine against O. tsutsugamushi would be more favorable for preventing scrub typhus in the endemic areas. Materials and Methods: We combined the immunoreactive regions of 47-kDa outer membrane protein (47-kDa OMP) and 56-kDa type-specific antigen (TSA56) to generate a conventional subunit vaccine expressing recombinant OTBS56-47 (recOTBS56-47) and a DNA vaccine (pOTBS56-47). We evaluated two types of vaccination, intranasal immunization with recOTBS56-47, and Intramuscular immunization with pOTBS56-47 for immunogenicity and efficacy. Results: In mice, intranasal immunization with recOTBS56-47 plus cholera toxin (CT) was the most effective method for the induction of humoral and cell-mediated immune responses; it induced a strong cellular immune response, as demonstrated by a spleen cell proliferation assay, and induced a higher amount of recOTBS56-47-specific antibodies. Intramuscular immunization with pOTBS56-47 alone, or pOTBS56-47 plus adjuvant pmIL-2, induced a low level of humoral immune response. Conclusion: OTBS56-47 is an attractive candidate for developing a prophylactic vaccine against scrub typhus caused by O. tsutsugamushi. This work was supported by an intramural fund (2013-NG52001-00) by research of Korea Centers for Disease Control and Prevention.