Ginsenoside Rg1 Induces Autophagy in Colorectal Cancer through Inhibition of the Akt/mTOR/p70S6K Pathway

Ruiqi Liu,Bin Zhang,Shuting Zou,Li Cui,Lin, Lin,Lingchang Li The Korean Society for Microbiology and Biotechnol 2024 Journal of microbiology and biotechnology Vol.34 No.4

This study aimed to elucidate the anti-colon cancer mechanism of ginsenoside Rg1 in vitro and in vivo. Cell viability rate was detected using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) tetrazolium assay. The inhibitory effect of ginsenoside Rg1 against CT26 cell proliferation gradually increased with increasing concentration. The in vivo experiments also demonstrated an antitumor effect. The monodansylcadaverine (MDC), transmission electron microscopy (TEM), and expression of autophagy marker proteins confirmed that ginsenoside Rg1 induced autophagy in vitro. Ginsenoside Rg1 induced autophagy death of CT26 cells, but this effect could be diminished by autophagy inhibitor (3-methyladenine, 3-MA). Additionally, in a xenograft model, immunohistochemical analysis of tumor tissues showed that the LC3 and Beclin-1 proteins were highly expressed in the tumors from the ginsenoside Rg1-treated nude mice, confirming that ginsenoside Rg1 also induced autophagy in vivo. Furthermoer, both in vivo and in vitro, the protein expressions of p-Akt, p-mTOR, and p-p70S6K were inhibited by ginsenoside Rg1, which was verified by Akt inhibitors. These results indicated that the mechanism of ginsenoside Rg1 against colon cancer was associated with autophagy through inhibition of the Akt/mTOR/p70S6K signaling pathway.

High-Pitch Coronary CT Angiography at 70 kVp Adopting a Protocol of Low Injection Speed and Low Volume of Contrast Medium

Ruiqi Feng,Jiajie Tong,Xiaofei Liu,Yu Zhao,Liang Zhang 대한영상의학회 2017 Korean Journal of Radiology Vol.18 No.5

Objective: To evaluate the feasibility and image quality (IQ) of prospectively high-pitch coronary CT angiography (CCTA) with low contrast medium injection rate at 70 kVp. Materials and Methods: One hundred and four patients with suspected coronary artery disease (body mass index < 26 kg/m2, sinus rhythm and heart rate < 70 beats/min) were prospectively enrolled and randomly divided into two groups. In group A and group B, 28 mL and 40 mL of 370 mgI/mL iodinated contrast media was administrated at a flow rate of 3.5 and 5 mL/s, respectively. CT values, noise, signal-to-noise ratio, contrast-to-noise ratio (CNR) of the proximal segments of coronary arteries and subjective IQ were evaluated. Results: The CT values and noise in group A were significantly lower than those in group B (434–485 Hounsfield units [HU] vs. 772–851 HU, all p < 0.001; 17.8–22.3 vs. 23.3–26.4, all p < 0.005). The CNRs of the right coronary artery and left main artery showed no statistical difference between the two groups (42.1 ± 13.8 vs. 36.8 ± 16.0, p = 0.074; 38.7 ± 10.6 vs. 38.1 ± 17.0, p = 0.819). No statistical difference was observed between the two groups in IQ scores (3.04 ± 0.75 vs. 3.0 ± 0.79, p = 0.526) and diagnostic ratio (96.1% [50/52] vs. 94.2% [49/52], p = 0.647). Conclusion: Prospective high-pitch CCTA at 70 kVp with 28 mL of contrast media and injection rate of 3.5 mL/s could provide diagnostic IQ for normal-weight patients with heart rate of < 70 beats/min.

Functional analysis of prv-miR-LLT11a encoded by pseudorabies virus

Huimin Liu,Li Yang,Zhibin Shi,Ruiqi Lv,Xia Yang,Chuanqing Wang,Lu Chen,Hongtao Chang 대한수의학회 2019 Journal of Veterinary Science Vol.20 No.6

Viral-encoded microRNAs (miRNAs) play have vital roles in the regulations of virus replications and host immune responses. The results of previous studies have indicated that miRNA clusters are involved in the replication and virulence of the pseudorabies virus (PRV), which may potentially lead to the immune escape or facilitation of PRV replications. This study's previous research revealed that the prv-mirmiR-LLT11a was differentially expressed during PRV infections. The present study's results have demonstrated that the prv-miR-LLT11a could significantly inhibit PRV replications. It was further determined that SLA-1 was the target gene of the prv-miR-LLT11a, and simultaneously, thate overexpression of prv-miR-LLT11a could down-regulate the mRNA and protein levels of SLA-1 in a dose-independent manner. Furthermore, the present study also found observed that the prv-miR-LLT11a canhad also down-regulated the TAP1 expressions. Our findings provide a better understanding of the molecular mechanism involved in on the effects of prv-miR-LLT11a on SLA-1 and TAP1, as well as and its involvement in a potential immune system evasion of PRV.

Application of UV Photocatalytic Degradation of Benzene

Gan, Yi,Liu, Ruiqi,Yu, Zhimin Urban Science Institute 2019 도시과학 Vol.8 No.2

Benzene pollution is becoming increasingly serious, and the treatment technology of benzene has attracted much attention. In this paper, a self-made photocatalytic reactor was used to explore the removal rate of benzene under the ultraviolet light with the wavelength of 253.7nm. The results showed that the degradation rate of benzene decreased from 64.29% to 16.26% when the concentration increased from 43mg/㎥ to 256mg/㎥ under the condition of 28W UV light intensity and 50s residence time. Under the condition of 28W UV light intensity and 103mg/㎥ concentration, the residence time increased from 16.5s to 50s, and the benzene removal rate increased from 13.23% to 42.72%.Under the condition of benzene concentration 103mg/㎥ and residence time of 50s, the removal rate of benzene increased from 29.34% to 52.58% in the process of UV light intensity rising from 28W to 48W.It is concluded that decreasing the concentration and increasing the residence time of gas were beneficial to the removal of benzene and increasing the light intensity can improve the removal rate of benzene.

Parameters Adaptive Control of Uncertain Chaotic Systems

Shujun Liang,Haiyan Liu,Ruiqi Wang,Junwei Lei 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.12

The bound of static uncertain function of chaotic system is required to be known by using adaptive method in many past research papers. Or many papers only considered the situation of the systems with simple uncertain nonlinear functions. In this paper, a kind of generalized chaotic systems are taken as an example, and an adaptive control algorithm with static uncertain nonlinear functions is proposed to control chaotic systems. The requirements for nonlinear function of system is relaxed by using the proposed algorithm. It only requires that the bound of the nonlinear functions in the system model exists and some part of it is known. At last, detailed numerical simulation is done to testify the rightness of the proposed method. It shows that the stabilization and tracking of chaotic system with static uncertain functions can be realized with a quick response by using the proposed algorithm.

Cyclic Loading Test for Concrete-Filled U-Shaped Steel Beam to Concrete-Filled Steel Tube Column Connections

Xing Xu,Rui Cheng,Pu Yang,Ruiqi Liu 한국강구조학회 2020 International Journal of Steel Structures Vol.20 No.6

Concrete-fi lled U-shaped steel (CFUS) beam and concrete-fi lled steel tube (CFST) column are recently developed because of the quick construction and economical saving. This paper aims to investigate the seismic performance of a composite connection system connecting CFUS beam to square CFST columns. In the joint system, the grooved cuts on the square tube allowed the U-shaped steel beam passing through the joint core completely, which developed a through beam connection. To verify the seismic behavior of the beam-column connection, three specimens designed according to the strong column-weak beam and strong joint-weak member seismic concept were tested under combined constant axial compression and cyclic loading. For the test parameters, the diff erent connection details and the axial load on the CFST column were considered. The test results showed that the specimens exhibited good load-carrying, deformation, and energy dissipation capacities. The maximum story drift ratio of all the three specimens was 5%, satisfying the minimum requirement of special moment frames. The primary failure mode of the specimens was local buckling of the webs and the fracture of the bottom fl anges of the CFUS beam near the column tube. In addition, the joint shear strength of the specimens was evaluated based on ASCE design guidelines.