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Wu Jun(Jun Wu),Luo Yuxin(Yuxin Luo) 아시아사회과학학회 2022 Jornal of Asia Social Science Vol.8 No.1
The problem of “shelling” of specialized farmers’ cooperatives is that farmers’ cooperatives neither insist cooperation essence nor farmer actual participation, and there is no normal operation. The “shelling” problem of farmers’ cooperatives in Guizhou Province is manifested in the three phenomena of “titular form”, “be more in name than in reality” and “unsustainable form”. For the unsustainable farmers’ cooperatives, we can take measures such as improving the organizational structure and management system, exploring joint construction mechanism among the rural credit cooperatives, supply and marketing cooperatives and farmers’ cooperatives, exploring technical services, strengthening the connection of interests and cultivating talent through multiple channels to solve the difficult problems existing in “organization, capital, market, talent, motivation” of the farmers’ cooperatives, achieving the high-quality development.
Intrusion Detection System based on Hidden Conditional Random Fields
Jun Luo,Zenghui Gao 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.9
Intrusion detection is an important way to ensure the security of computers and networks. In this paper, a new intrusion detection system (IDS) is proposed based on Hidden Conditional Random Fields (HCRFs). In order to optimize the performance of HCRFs, we bring forward the Two-stage Feature Selection method, which contains Manual Feature Selection method and Backward Feature Elimination Wrapper (BFEW) method. The BFEW is a feature selection method which is introduced based on wrapper approach. Experimental results on KDD99 dataset show that the proposed IDS not only has a great advantage in detection efficiency but also has a higher accuracy when compared with other well-known methods.
Luo Juan,Bai Luyan,Tao Jun,Wen Yu,Li Mingke,Zhu Yunzhen,Luo Sufeng,Pu Guangyu,Ma Lanqing 한국유전학회 2021 Genes & Genomics Vol.43 No.10
Background Vacuolating cytotoxin (VacA) is an important virulence factor of Helicobacter pylori (H. pylori). It was previously believed that VacA can trigger the cascade of apoptosis on mitochondria to lead to cell apoptosis. Recently, it was found that VacA can induce autophagy. However, the molecular mechanism by which VacA induces autophagy is largely unknown. Objective We aimed to explore the molecular mechanism of autophagy induced by H. pylori in gastric cancer cells and the efect of autophagy on the survival of gastric cancer cells. Methods The autophagy of human gastric cancer cell line SGC7901 was detected by Western blot and RT-PCR in the treatment of VacA protein of H. pylori. The relationship between autophagy and reactive oxygen species (ROS) in the proliferation of gastric cancer cells were studied by gene expression silences (siRNA) and CM-H2DCFDA (DCF) staining. Results The results showed that VacA protein secreted by H. pylori in the supernatant stimulated autophagy in SGC7901 cells. After VacA protein treatment, the mRNA expressions of BECN1, ATG7 and PIK3C3, were up-regulated. ATG7 silencing by siRNA inhibited VacA-induced autophagy. Furthermore, our data demonstrated that VacA protein increased ROS levels. Addition of the antioxidant N-acetyl-l-cysteine (NAC) suppressed the levels of ROS, leading to inhibition of autophagy. Conclusions H. pylori VacA is a key toxin that induces autophagy by increased ROS levels. And our fndings demonstrated that VacA signifcantly inhibited proliferation in SGC7901 cells.
Jun Luo,Junying Liu,He-he Liu,Tao Zhang,Jiwen Wang,Hua He,Chunchun Han 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.7
Objective: The bursa of Fabricius (BF) is a central humoral immune organ belonging specifically to avians. Recent studies had suggested that miRNAs were active regulators involved in the immune processes. This study was to investigate the possible differences of the BF at miRNA level between two genetically disparate duck breeds. Methods: Using Illumina next-generation sequencing, the miRNAs libraries of ducks were established. Results: The results showed that there were 66 differentially expressed miRNAs and 28 novel miRNAs in bursa. A set of abundant miRNAs (i.e., let-7, miR-146a-5p, miR-21-5p, miR-17~92) which are involved in immunity and disease were detected and the predicted target genes of the novel miRNAs were associated with duck high anti-adversity ability. By gene ontology analysis and enriching KEGG pathway, the targets of differential expressed miRNAs were mainly involved in immunity and disease, supporting that there were differences in the BF immune functions between the two duck breeds. In addition, the metabolic pathway had the maximum enriched target genes and some enriched pathways that were related to cell cycle, protein synthesis, cell proliferation and apoptosis. It indicted that the difference of metabolism may be one of the reasons leading the immune difference between the BF of two duck breeds. Conclusion: This data lists the main differences in the BF at miRNAs level between two genetically disparate duck breeds and lays a foundation to carry out molecular assisted breeding of poultry in the future.
Luo Jian-jun 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.6
A numerical simulation on the 3D compressible viscous unsteady flow field induced by a high-speed subway train, while it’s passing by the platform screen door of subway station, is presented in the paper. The research on transient pressure in tunnel due to changes of positions of platform screen door is proceeded in order to design platform screen doors for underground stations of Dong-guan to Huizhou inter-city rail traffic. Result shows that the platform is subjected to aerodynamic compression and tension cycle loads all the time. And compression load is larger than tension load. The maximum and minimum pressure values in absolute terms got from measuring points of the whole station decrease gradually along the direction train going ,no matter whether the platform screen door is fixed or not. The absolute values of maximum and minimum pressure of piston wind generated at platform screen door are decreasing while the spacing between platform screen door and platform becomes wider. The pressure value of air at exit is larger than that of air at entrance. As for a high-speed subway station, it is a practicable way to set air shafts at entrance and exit of station to mitigate pressure of air in station and tunnel. The pressure value of air could have a 10 to 15 percent reduction if there is an air shaft. Besides, the platform screen door can be installed in half tall of PSD.
Luo, Guo-Xuan,Cai, Jun,Lin, Jing-Zhi,Luo, Wei-Shi,Luo, Heng-Shan,Jiang, Yu-Yang,Zhang, Yong Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12
Objective: To investigate the effects of gambogic acid (GA) on the growth of human malignant glioma cells. Methods: U251MG and U87MG human glioma cell lines were treated with GA and growth and proliferation were investigated by MTT and colony formation assays. Cell apoptosis was analyzed by annexin V FITC/PI flow cytometry, mitochondrial membrane potential assays and DAPI nuclear staining. Monodansylcadaverine (MDC) staining and GFP-LC3 localisation were used to detect autophagy. Western blotting was used to investigate the molecular changes that occurred in the course of GA treatment. Results: GA treatment significantly suppressed cell proliferation and colony formation, induced apoptosis in U251 and U87MG glioblastoma cells in a time- and dose-dependent manner. GA treatment also lead to the accumulation of monodansylcadaverine (MDC) in autophagic vacuoles, upregulated expressions of Atg5, Beclin 1 and LC3-II, and the increase of punctate fluorescent signals in glioblastoma cells pre-transfected with GFP-tagged LC3 plasmid. After the combination treatment of autophagy inhitors and GA, GA mediated growth inhibition and apoptotic cell death was further potentiated. Conclusion: Our results suggested that autophagic responses play roles as a self-protective mechanism in GA-treated glioblastoma cells, and autophagy inhibition could be a novel adjunctive strategy for enhancing chemotherapeutic effect of GA as an anti-malignant glioma agent.
Numerical Simulation of Magnetorheological Liquid Sedimentation Character
Jun Qiu,YiPing Luo,Jiao Luo,Ying Wang,Hongyi Wang 한국자기학회 2020 Journal of Magnetics Vol.25 No.3
In this paper, numerical simulation is used to simulate the sedimentation process of magnetorheological fluid (MRF), and the sedimentation time of MR fluid is predicted by this method. Firstly, based on the existing literature simulation method and its improvement, a numerical simulation method is proposed to simulate the MR fluid sedimentation process. Finally, a variety of MR fluid samples are prepared to detect the accuracy of the simulation results of the constructed numerical simulation method. It can be proved by comparing the measured sedimentation time with the simulated sedimentation time, and the method constructed in this paper can predict the sedimentation time of the more complicated MR fluid well, which has good practicability.