능이버섯의 藥理作用에 관한 硏究

殷載淳,이중원,黃甲洙 又石大學校 1990 論文集 Vol.12 No.-

The pharmacological effects of Sarcodon aspratus extract were investigated. The extract promoted contraction of isolated stomach and ileum, and this contraction was blocked by atropine(7×10^-7M) and cyproheptadine (1.5×10^-7M). The maximum effective dose of the extract was 1.5×10^-3g/㎖. The extract promoted the peristaltic movement of small intestine, and this movement was blocked by atropine. The extract accelerated the secretion of histamine in serum and gastric acid. The serum glucose and total cholesterol levels in alloxan-induced mice were significantly decreased by oral administration of the extract for 7 days. The pharmacological effects of the extract on the central nervous system were little.

Piperic Acid Amide 誘導體의 微生物學的 硏究 : Studies on the Synthesis and Antibacterial Activity of Piperic Acid Analogues

殷載淳 又石大學校 1983 論文集 Vol.5 No.-

5-(3, 4-methylenedioxypheny)-2, 4-pentadienoic acid[piperic acid], 3, 4-methylenedioxy cinnamic acid, 3, 4-methylenedioxy benzoic acid, 5-phenyl 2, 4-pentadienoic acid, cinnamic acid, benzoic acid and various amides and esters of these acids were synthesized. The fifty-six compounds synthesized were tested for their antibacterial activity was shown as follows;the number of synthesized compounds, ⅩⅩⅩⅤ, ⅩⅩⅩⅨ, ⅩL, ⅩLⅠ, ⅩLⅢ, ⅩLⅥ, ⅩLⅨ and LⅢ were active against Staphylococcus aureus, ⅩⅩⅩⅨ, ⅩL, ⅩLⅥ and ⅩLⅨ were active against Bacillus subtilis, ⅩⅠ, ⅩⅩⅡ, ⅩⅩⅢ, ⅩⅩⅥ, ⅩⅩⅦ, ⅩⅩⅩⅧ, ⅩⅩⅩⅨ, ⅩLⅥ, LⅩⅧ, ⅩLⅨ, LⅡ, LⅣ and LⅥ were native against Escherichia coli, and ⅩⅩⅩⅥ, ⅩⅩⅩⅦ, ⅩLⅥ, ⅩLⅦ, ⅩLⅧ, LⅣ, LⅤ and LⅥ were active against Pseudomonas aeruginosa.

甘草엑스의 抗炎 및 抗潰瘍作用에 대한 機轉

殷載淳,崔東晟,全焄,安文生 又石大學校 1989 論文集 Vol.11 No.-

Glycyrrhizae radix, commonly called licorice, has been used as anti-ulcerous and anti-inflammatory agents. It's main component, glycyrrhizin, is known to be effective as an anti-allergenic, anti-hepatic, and anti-viral agent, and shows steroid-like action. Histamine mediates and modulates inflammation through a variety of immuno-regulatory actions and stress-induced secretion of glucocorticoids(GC) is generally mediated by release of ACTH from the pituitary gland. This study was to investigate the mechanism of Glycyrrhizae Radix Ext. on anti-inflammatory and anti-ulcerous action. 1. The serum histamine contents was the most increased at the dose of 25㎍/㎏ LPS. injected rats(i.p), and the serum corticosterone was not significant. 2. Pretreatment of Glycyrrhizae radix Ext. (150㎎/㎏) was markedly decreasing the levels of serum histamine and corticosterone. 3. The serum histamine content was significantly increased in 120 minutes after LPS. injection. 4. The serum histamine content was decreased by pretreatment of cimetidine (H^2- receptor antagonist) 5㎎/㎏. These results were suggested that the effects of Glycyrrhizae Radix Ext, on anti-inflammatory and anti-ulcerous action was induced by its glucocorticoid-like action and inhibition of the histamine realese via H_2-receptor.

3, 4-Methylenedioxy Cinnamic Acid Amide 誘導體의 合成 및 藥理作用에 관한 硏究

殷載淳 又石大學校 1984 論文集 Vol.6 No.-

In order to search more active and less toxic compound in comparison with the central nervous depressant activity of piperine, 3,4-methylenedioxy cinnamic acid amides were synthesized by condensation of 3,4-methylenedioxy cinnamoyl chloride with alkyl or aryl amines, and N-acyl-3,4-methylenedioxy cinnamimides were synthesized by condesation of 3,4-methylenedioxy unsubstiuted amide with acyl chlorides. The bioassay results revealed that, among the compounds synthesized, 3,4-methylenedioxy cinnamoyl monoethylamide exhibited more potent protective activity against pentylenetetrazoleinduced convulsion, 3,4-methylenedioxy cinnamoyl unsubstituted amide, monoethylamide and anilide exhibited more potent protective activity against strychnine-induced convulsion, and all of the compunds except 3,4-methylenedioxycinnamoyl unsubstituted amide and monoethylamide showed less acute toxicity than that of piperine.

청피에 함유된 복강 파크로파지의 탐식작용 억제 성분

은재순,김대근,소준노,지옥표 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

The phagocytic activity of murine peritoneal macrophage was determined by lucigenin chemiluminescence and engulfment of fluorescein-conjugated E. coli particles. The activity-guided fractionation upon the methylenechloride fraction of Aurantii immaturi pericarpium led to the isolation of a flavonoid, isosinensetin, as a suppressive component of phagocytosis, lsosinensetin suppressed the lucigenin chemiluminescence and the engulfment of fluorescein-conjugated E. coli particles and enhanced the production of nitric oxide in murine peritoneal macrophage.

3T3-L1 세포주에서 분비하는 인체 암세포 성장억제 단백질에 대한 연구

은재순,권진 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

Ingibition of the growth of human cancer cells by proteins secreted from 3T3-L1 cells was investigated in the present study. The growth of human cancer cells was inhibited by co-culture with 3T3-L1 cells under 10% FBS and DME, GIT and serumless medium, respectively. The conditioned medium of cultured 3T3-L1 cells under serumless medium was concentrated 100-fold through an ultrafiltration cell with a 10,000 molecular weight cutoff at 4℃ under positive pressure using nitrogen (3T3-L1 EM). 3T3-L1 EM inhabited the growth of HrRa, Hep G2, KHOS-Np, A431 andMCF-7 cells. 3T3-L1 EM was purified with FPLC, DEAE-ion exchange chromatography and pheny-sepharose chromatography. The major protein of 3T3-L1 EM has a molecular weight of 66,000-69,000 in SDS-PAGE analysis. The results suggest that the inhibitory activity of 3T3-L1 EM appears to be due to some protein(m.w. 66,000-68,000) secreted by 3T3-L1 cells.

사군자탕이 L1210 세포를 이식한 마우스의 면역세포에 미치는 영향

殷載淳,金大根,柳東和,權鎭,徐龍勳,蘇俊魯,全焄,吳贊鎬 우석대학교 의약품개발연구소 1997 藥學硏究誌 Vol.2 No.-

The purpose of this research was to investigate effects of Sa-Kunja-Tang(SKT) on immune cells of L1210 cell-transplanted mice. The apoptosis and T lymphocytes subopoulation were tested using a flow cytometry, and the proliferation was tested using a MTT assay. Nitric oxide production from mouse peritoneal macrophage was tested using a Griess reagents, and the phagocytic activity of mouse peritoneal macrophage was tested using a lucigenin chemiluminescence. SKT suppressed apoptosis of T-lymphocytes induced by L1210 transplantation. SKT decreased nitric oxide production from mice peritoneal macrophages increased by L1210 transplantation, and the phagocytic activity decreased by L1210 transplantation. These results suggest that SKT suppresses T lymphocyte apoptosis and macrophage activity in L1210 transplanted mice.

托裡消毒飮이 傷處治癒에 미치는 影響

殷載淳,黃甲洙 又石大學校 1992 論文集 Vol.14 No.-

These experiments were conducted to investigate the wound healing action of Taklee-Sodok-Eum(TSE) water ext. TSE extract did not affect on the paper disk granuloma formation and mouse paw edema induced by histamine, but inhibited on the leakage of evans blue into peritoneal cavity. Using proliferation of Balb/c 3t3 fibroblast cell line as an in vitro model of granulation tissue formation, the ability of TSE to stimulate cellualar proliferation of fibroblast cells was investigated. When the cells were seeded at 1 × 10 exp (4) cells/well, Balb/c 3T3 cells were reached to the late exponential phase at 3rd day. Under the conditions established above, TSE increased the proliferation of Balb/c 3T3 cells at concentration of 10^-6 g/ml. The treatment of 10^-6 g/ml of TSE did not influence on the DNA synthesis, but decreased the protein synthesis of the cells. The 10% serum from TSE treated mice(500㎎/㎏/day for 4 days) increased the proliferation of Balb/c 3T3 fibroblast markedly, and did not influence on the DNA synthesis and protein sythesis of the cells. The results suggest that wound healing in vivo will be agumented by the treatment of TSE. In addition, TSE may be of practical therapeutic use in wound healing.

일할 수밖에 없는 여성노인의 다중차별 경험에 관한 연구

정순둘,장명선,이은진,송보가,김재민 이화여자대학교 젠더법학연구소 2021 이화젠더법학 Vol.13 No.2

본 연구는 노동시장에서 일할 수밖에 없는 여성노인의 다중차별 경험을 파악하여 이들의 노동권을 보호하고 차별을 해소하기 위한 방안을 마련하는데 목적이 있다. 이를 위해 노인집중 고용 업종에 종사하는 60세 이상 여성노인 14명을 대상으로 심층면접조사를 진행하였다. 본 연구 결과, 일할 수밖에 없는 여성노인들은 노동시장의 입직 단계에서부터 성별, 연령, 건강의 다중차별에 의한 상시적인 고용불안과 사회안전망에서의 배제를 경험하였다. 또한 일하는 조직의 불평등 지배체제 특성으로 일상화된 고용불안, 열악한 노동조건과 복지제도의 미비, 노인친화적이지 않은 노동 강도와 근무 제공, 노인 노동 평가 절하에 따른 조직 위계 문화와 관행, 과도한 노동 제공의 요구와 성희롱의 일상화, 조직 내에서 이해를 대변하는 데 어려움을 경험하는 것으로 나타났다. 본 연구 결과를 바탕으로 일할 수밖에 없는 여성노인의 다중차별을 해소하기 위한 법과 제도의 개선, 사회안전망 강화, 여성노인 노동자 이해대변단체 조직화 지원을 제언하였다. This study aims to (i) identify the experience of multiple discrimination among older women, who have no choice but to work in the labor market; (ii) protect their labor rights; and (iii) resolve discrimination. To conduct this study, we performed an in-depth interview survey with 14 older women aged 60 or older who are engaged in the elderly intensive employment sector. Under the result of this study, older women, who have no choice but to work, have experienced constant employment insecurity and exclusion from the social safety net due to multiple discrimination in gender, age, and health from the employment stage in the labor market. In addition, because of inequality organizations, they have experienced as follows: employment insecurity that became common, poor labor conditions and inadequate welfare systems, unfriendly labor intensity and provision of work, organizational hierarchical culture and practices due to devaluation of elderly labor, demand for excessive labor provision and sexual harassment routinely, and difficulties in representing understanding within the organization. Based on the results of this study, therefore, this study provides suggestions to resolve the multiple discrimination of older women, who have no choice but to work, including but no limited to, improve laws and systems, strengthen the social safety net, and organize support for the organization of interest groups for older women workers.

子官癌細胞(HeLa)에 대한 血府逐瘀湯과 數種 抗癌劑의 倂用投與 效果

殷載淳,蘇俊魯 又石大學校 1992 論文集 Vol.14 No.-

The studies were conducted to investigate the combined effects of Hyulbu Chuko Tang (HCT) and several anti-cancer drugs. The effects of HCT and several anti-cancer drugs on the proliferation of HeLa Colls, human cervical cell line, was estimated by MTT colorimetric assays. The 50% inhibitory concentrations of mitomycin C(MMC), cisplatin(CPT), mercaptopurine (MCP) and 5-fluorouracil(5-FU) on HeLa cell were 0.313, 0.565, 22.745 and 28.325/㎖, repectively. HCT extract inhibited the proliferation of HeLa cell at 10^-6-10^-3g/㎖. The inhibitory action of the MCP treated group on the proliferation of HeLa cell was increased by HCT and MCP. When the mice were treated by MMC, the number of leukocyte was decreased significantly at the 1st and 3rd day, but recovered at the 7th day. In the groups of MMC treated with HCT, the number of leukocytes was increased significantly than the group of MMC treated only at the 1st and 3rd day. The HCT extract increased the number of plaque forming cells(PFC), but the MMC treated group decreased the number of PFC. The combined treatment of MMC and HCT increased the number of PFC significantly than the MMC treated group. The HCT extract increased the proliferation of T cells, but the MMC treated group decreased the proliferation of T cells. The combined treatment of MMC and HCT increased the T cell proliferation significantly than MMC treated group.