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"송혜인", "박동원", "안성옥", "김수균" 배재대학교 공학연구소 2015 공학논문집 Vol.17 No.1
모바일 게임은 간단한 조작과 짧은 라운드 형식으로 바쁜 현대인들을 위한 스트레스 해소용 게임으로써의 역할을 톡톡히 해내고 있다. 그 중에서도 자동실행 없이 직접 조작하여 게임에 집중하는 정도에 따라 점수를 매겨 성취감을 이끌어 낼 수 있는 러닝 어드벤처 게임이 몰입도가 높아 짧은 시간동안 힘든 일을 잊게 해주는 역할을 더욱 더 효율적으로 해낼 수 있다. 따라서 본 논문에서는 Unity 3D 엔진의 NGUI 플러그인을 이용하여 모바일 환경에서 간단한 조작으로 가볍게 실행시킬 수 있는 2D 러닝 어드벤처 게임을 설계하는 것을 목표로 한다. Mobile games are rolled into the as important games for the busy modern man with a simple operation. Among them is also the direct operation without Auto run ranked a score based on the degree to concentrate on the game be able learning adventure game that has led to the accomplishment commitment can do a role that you forget the hard work for a short period of time increased more efficiently. In this paper, using the engine of NGUI plug of Unity 3D, it aims to design a 2D-learning adventure game that can execute it with a simple operation in a mobile environment.
Translocation of DNA and protein through a sequentially polymerized polyurea nanopore
Kim, Hyung-Jun,Choi, Ui-Jin,Kim, Hyein,Lee, Kidan,Park, Kyeong-Beom,Kim, Hyun-Mi,Kwak, Dong-Kyu,Chi, Seung-Wook,Lee, Jin Seok,Kim, Ki-Bum The Royal Society of Chemistry 2019 Nanoscale Vol.11 No.2
<P>Here, we investigated the translocation of biomolecules, such as DNA and protein, through a sequentially polymerized polyurea nanopore, with a thin (<10 nm) polymer membrane of uniform thickness. The polyurea membrane was synthesized by molecular layer deposition using <I>p</I>-phenylenediisocyanate (PDI) and <I>p</I>-phenylenediamine (PDA) as sequential precursors. The membrane exhibited a hydrophobic surface with a highly negative surface charge density (−51 mC m<SUP>−2</SUP> at pH 8). It was particularly noted that the high surface charge density of the membrane resulted in a highly developed electro-osmotic flow which, in turn, strongly influenced the capture probability of biomolecules, depending on the balance between the electro-osmotic and electrophoretic forces. For instance, the capture frequency of negatively charged DNA was demonstrated to be quite low, since these two forces more or less cancelled each other, whereas that of positively charged MDM2 was much higher, since these two forces were additive. We also identified that the mean translocation time of MDM2 through the polyurea nanopore was 26.1 ± 3.7 μs while that of the SiN nanopore was 14.2 ± 2.0 μs, hence suggesting that the enhanced electrostatic interaction between positively charged MDM2 and the negatively charged pore surface affects the translocation speed.</P>
( Hyein Kim ),( Seul Ki Kim ),( Chang-woo Choo ),( Jung Ryeol Lee ),( Byung Chul Jee ),( Won Don Lee ),( Seok Hyun Kim ) 대한산부인과학회 2018 대한산부인과학회 학술대회 Vol.104 No.-
Objective: To investigate the recognition and knowledge about oocyte freezing for social or medical reasons among unmarried women of reproductive age in Korea. Methods: This was a survey study performed at a single center between December 2016 and May 2018. Women of reproductive age (20-45 years) who had no partner at the time of recruitment were included. The 30-item questionnaire consisted of following parts: (1) demographics; (2) awareness of pregnancy and childbirth; (3) recognition about oocyte freezing and factors that affect a woman's decision-making in the utilization of fertility preservation; and (4) knowledge about oocyte freezing. Results: 86 women received an electrical survey and 15 were excluded as they were incomplete. Mean age was 37.0 years. Over half of the women (73%) responded they decided to undergo oocyte freezing for their fertility preservation. Most frequently reported reason for the decision was to maintain their fertility in the future when they attempt pregnancy (70.6%). Most of the respondents (61.8%) expressed positive intent for oocyte donation. In the evaluation of background knowledge about fertility, most respondents got only 1 question correct out of the 6. Most respondents thought that costs for the procedure should be covered by medical insurance or social security system. They expected proper medical advice about fertility preservation when they met health care providers. Conclusion: Interest in the preservation of freezing eggs has increased and the number of procedures is increasing in Korea, but it has not been universal yet. Reinforcement in governmental and institutional support is essential to meet the demand of women who want to preserve their fertility. This could be one of the solutions to the low fertility crisis in Korea.
Shin, HyeIn,Kim, Teayeop,Seo, IlHo,Kim, SeonIl,Kim, Yong Jae,Hong, Hyeonaug,Park, Yunjeong,Jeong, Hyung Mo,Kim, Kyunghoon,Ryu, WonHyoung Elsevier BV * North-Holland 2019 Applied Surface Science Vol.481 No.-
<P><B>Abstract</B></P> <P>For extraction of photosynthetic electrons (PEs) from plant cells and algal cells, there have been many approaches using living algal cells or isolated photosynthetic apparatus such as photosystem II, photosystem I, and thylakoid membranes (TMs). Among these, bio-photoanodes coated with TMs demonstrated stable performance and the possibility for their practical applications. When a TM photoanode is prepared, TMs are deposited on the surface of a metal electrode. However, since the thickness of TM films determines the light absorption and electron transfer processes, the performance of a TM bio-photoanode is significantly affected by the TM film quality. Thus, in this study, electrospraying was employed to deposit TMs with enhanced control of the thickness and uniformity of TM coating on metal electrodes. In particular, we investigated how both the quality of TM films and the magnitude of PE currents were influenced by electrospraying time, substrate motion, solution feed rates, TM concentration, and addition of graphene oxide (GO) nanosheets. Finally, to assess the feasibility of TM electrospraying as a scalable fabrication method, TMs were electrosprayed on 5 × 5 cm<SUP>2</SUP> size films of indium tin oxide (ITO)-coated polyethylene naphthalate (PEN) (ITO-PEN). The coating uniformity was assessed by measuring PE currents from different locations of the TM-deposited ITO-PEN films.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Electrospraying was employed to uniformly deposit thylakoid/graphene oxide(GO) composite on electrodes for enhanced photosynthetic currents </LI> <LI> GO addition to thylakoid solution enhanced charge transfer between thylakoid and GO nanosheets and resulted in higher photosynthetic currents </LI> <LI> Flexible large-area bio-photoanodes (5 × 5 cm<SUP>2</SUP>) with high uniformity were fabricated by electrospraying thylakoids on ITO-PEN films </LI> </UL> </P>
Lactobacillus Persisters Formation and Resuscitation
( Hyein Kim ),( Sejong Oh ),( Sooyeon Song ) 한국미생물생명공학회 2024 Journal of microbiology and biotechnology Vol.34 No.4
Lactobacillus is a commonly used probiotic, and many researchers have focused on its stress response to improve its functionality and survival. However, studies on persister cells, dormant cells that aid bacteria in surviving general stress, have focused on pathogenic bacteria that cause infection, not Lactobacillus. Thus, understanding Lactobacillus persister cells will provide essential clues for understanding how Lactobacillus survives and maintains its function under various environmental conditions. We treated Lactobacillus strains with various antibiotics to determine the conditions required for persister formation using kill curves and transmission electron microscopy. In addition, we observed the resuscitation patterns of persister cells using single-cell analysis. Our results show that Lactobacillus creates a small population of persister cells (0.0001-1% of the bacterial population) in response to beta-lactam antibiotics such as ampicillin and amoxicillin. Moreover, only around 0.5- 1% of persister cells are heterogeneously resuscitated by adding fresh media; the characteristics are typical of persister cells. This study provides a method for forming and verifying the persistence of Lactobacillus and demonstrates that antibiotic-induced Lactobacillus persister cells show characteristics of dormancy, sensitivity of antibiotics, same as exponential cells, multi-drug tolerance, and resuscitation, which are characteristics of general persister cells. This study suggests that the mechanisms of formation and resuscitation may vary depending on the characteristics, such as the membrane structure of the bacterial species.