딥러닝을 활용한 차세대 주차관리 시스템 개발

이희원(Hee-Won Lee),정규철(Kyu-chul Jung),이현정(Hyun-Jung Lee),손혜원(Hye-Won Son),윤희진(Hee-Jin Yoon),안상태(Sangtae Ahn) 한국정보기술학회 2022 Proceedings of KIIT Conference Vol.2022 No.12

본 연구에서는 수요에 비해 작은 주차 부지를 최대한으로 활용하기 위하여 딥러닝 기반 차세대 주차관리 시스템을 개발하였다. 수행된 연구의 결과는 토대로 대형 쇼핑몰 등 유동 인구가 많은 상업지역에서의 활용할 수 있고, 주차 관리와 관련된 다양한 기술의 발전을 촉진할 수 있을 것이다. In this work, we develop a next-generation parking management system using deep learning to optimize limited parking lots. Based on the results of this work, we expect that the developed parking management system could be used for commercial areas such as shopping malls and accelerating the technologies related to parking management.

Efficient Transmission and Propagation of Tomato Chlorosis Virus by Simple Single-Leaflet Grafting

Lee, Huin,Kim, Mi-Kyeong,Choi, Hong-Soo,Kang, Jin-Ho,Ju, Ho-Jong,Seo, Jang-Kyun The Korean Society of Plant Pathology 2017 Plant Pathology Journal Vol.33 No.3

Tomato chlorosis virus (ToCV), a member of the genus Crinivirus, has caused an epidemic disease in tomato worldwide. ToCV is phloem-limited and transmitted by whiteflies in a semi-persistent manner, but not by mechanical inoculation. Experimental propagation of ToCV has been performed primarily by using whitefly-mediated inoculation. To develop a simple and convenient method for transmission of ToCV, we investigated grafting single-leaflets from tomato plants infected with ToCV to recipient tomato seedlings. Forty-one of 46 tomato seedlings tested were grafted successfully with single-leaflets infected with ToCV. Among them, 36 seedlings (87.8%) were systemically infected with ToCV and developed typical symptoms. Our results demonstrated that single-leaflet grafting could provide a sufficient amount of inoculum for the transmission of ToCV to the grafted seedlings.

임상연구 : 무수혈 심장 수술에 대한 임상적 고찰

이종현 ( Jong Hyun Lee ),신다흰 ( Da Huin Shin ),허금주 ( Gum Jn Hoo ),이창하 ( Chang Ha Lee ),나찬영 ( Chan Young Na ) 대한마취과학회 2007 Korean Journal of Anesthesiology Vol.52 No.5

Background: Jehovah`s Witnesses are well known for their refusal of blood transfusions which, challenges the safety of performing cardiac surgeries. Their stand regarding blood transfusions has garnered criticism from some medical and legal sources, but has also added incentive for the development of many bloodless surgery techniques. To assess the impact on the continuing progress of blood saving protocols and increasing operative risk; herein our results in this specific population are reported. Methods: The medical records of Jehovah`s Witnesses, who underwent cardiac surgeries without blood transfusions, between Feb 1996 and Sep 2005, were retrospectively reviewed. Results: 103 surgical procedures were performed on 102 patients. The age of patients varied from 2 months to 78 years, with a sex ratio of 60:42 (Male:Female). Cardiopulmonary bypass (CPB) was used in 81 cases. The mean hemoglobin (Hb) and hematocrit (Hct) levels were 13.2 g/dl (8.1-17.3), and 39.2% (24.3-52.5) before surgery, and 11.8 g/dl (8.0-16.5), and 35.3% (24-49) after surgery, respectively. Recombinant human erythropoietin (rHuEPO) and iron were used in 95 and 69 cases before and after surgery, respectively. High dose aprotinin (2 million KIU IV loading dose, 2 million KIU into the pump prime volume and 500,000 KIU per hour of surgery as a continuous intravenous infusion) was used in 67 cases. Acute normovolemic hemodilutions were performed in 7 cases. Cell saver was used in all procedures. Re-operations were needed in two cases due to wound infections and one patient died-of arrhythmias on the 2nd post-operative day. Conclusions: Bloodless cardiac surgery can be performed on Jehovah`s Witnesses, but effective care of such patients requires close collaborative team efforts and advance planning to ensure favorable outcomes. At our hospital, preoperative iron and rHuEPO, as well as high dose aprotinin and cell saver are routinely used where indicated. (Korean J Anesthesiol 2007; 52: 530~6)

식물 바이러스병 진단방법에 관한 고찰

이은혜 ( Eun-hye Lee ),윤선희 ( Seon-hee Yun ),( Huin Lee ),주호종 ( Ho-jong Ju ) 전북대학교 농업과학기술연구소 2022 농업생명과학연구 Vol.53 No.1

Although plant viruses are very small compared to other pathogen groups, they cause diseases in plants resulting in great quantitative and qualitative loss It causes losses of more than $30 billion annually worldwide. Since viral pathogens are absolute parasites in cells, direct control using chemicals is very difficult. Especially, once it occurred, it is almost impossible to get rid of viruses from infected plant in the field. Therefore, prevention of the virus spread through diagnosis is the best approach to control viral diseases so far. Here we compared diagnostic methods based on serological methods and molecular biological methods. Serological diagnostic methods include enzyme-linked immunosorbent assay (ELISA), dot blot immunobinding assay (DBIA), and tissue blot immunobinding assay (TBIA). Use of serological diagnostic method is gradually decreasing because It has disadvantages over molecular biological based diagnostic methods in terms of time required for diagnosis, false positives due to cross-reaction, and sensitivity. On the other hand, PCR-based molecular biological diagnostic methods are on the rise. PCR-based methods are divided into two groups. One is the traditional PCR system which need temperature change during amplification. The other is isothermal-based amplification system which is not required for temperature change. Many isothermal amplification methods have been developed, including Nucleic acid sequence-based amplification (NASBA), Loop-mediated isothermal amplification (LAMP), Recombinase polymerase amplification (RPA), etc. Traditional PCR based methods require expensive equipment, but isothermal-based amplification does not require expensive equipment. Isothermal diagnostic methods can be usefully in underdeveloped countries. Among isothermal amplification methods, RPA is most likely to be applied to on-site diagnosis due to its very short diagnosis time and excellent sensitivity. In conclusion, since accurate and fast detection system is an essential to control plant viruses, the development of betted diagnostic methods than the current existing diagnostic method should be continued, and that will greatly contribute to the control of plant viral diseases.

Cellular uptake mechanism and comparative <i>in vitro</i> cytotoxicity studies of monomeric LMWP-siRNA conjugate

Ye, Junxiao,Pei, Xing,Cui, Hui,Yu, Zhili,Lee, Hyukjin,Wang, Jianxin,Wang, Xu,Sun, Lu,He, Huining,Yang, Victor C. THE KOREAN SOCIETY OF INDUSTRIAL AND ENGINEERING 2018 JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY -S Vol.63 No.-

<P><B>Abstract</B></P> <P>The covalent attachment of CPPs to siRNA molecules offers great potential for CPP-mediated siRNA delivery. We recently reported a concise and high-yield synthesis strategy of the cell-permeable, cytosol-dissociable LMWP-siRNA covalent conjugate. Herein, cell uptake mechanism and cellular toxicity studies of this conjugate were performed to evaluate the potential of LMWP-siRNA conjugate for clinical translation. Cellular uptake mechanism study indicated that the conjugate could be taken up by cells via multiple pathways, including direct penetration of the plasma membrane and clathrin- and caveolae-independent endocytosis. <I>In vitro</I> cytotoxicity study revealed that the conjugation promoted internalization in a low-toxic fashion.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

MMP-2-responsive fluorescent nanoprobes for enhanced selectivity of tumor cell uptake and imaging

Sun, Lu,Xie, Shuping,Ji, Xiuru,Zhang, Jingming,Wang, Dongmei,Lee, Seung Jin,Lee, Hyukjin,He, Huining,Yang, Victor C. The Royal Society of Chemistry 2018 Biomaterials Science Vol.6 No.10

<P>It is difficult to develop highly selective substrate-based fluorescent nanoprobes for specific matrix metalloproteinases (MMPs) due to overlapping substrate specificities among the family of MMP enzymes. To resolve this issue, we have developed novel fluorescent nanoprobes that are highly selective for soluble MMP-2. Herein, MMP-2-responsive nanoprobes were prepared by immobilizing fluorescent fusion proteins on nickel ferrite nanoparticles <I>via</I> the His-tag nickel chelation mechanism. The fusion protein consisted of a fluorescent mCherry protein with a cell penetrating peptide (CPP) moiety. An MMP-2 cleavage site was also introduced within the fusion protein, which was directly linked to the nickel ferrite nanoparticles. The selectivity of nanoprobes was modulated by hiding the cleavage site of MMP-2 substrates deeply inside the system, which could result in strong steric hindrance between the nanoprobes and MMPs, especially for membrane-tethered MMPs such as MMP-14. A cell-based assay demonstrated that the nanoprobes could only be activated by tumor cells secreting soluble MMP-2, but not membrane-tethered MMP-14. To further evaluate the contribution of the steric hindrance effect on the nanoprobes, a truncated recombinant MMP-14 was employed to confer their cleavage activity as compared to native membrane-tethered MMP-14. Furthermore, a designed probe with a diminished steric hindrance effect was proved to be activated by membrane-tethered type MMP-14. The results indicated that the design of fluorescent nanoprobes employing the steric hindrance effect can greatly enhance the selectivity of MMP-responsive nanoprobes realizing the specific detection of soluble MMP-2 in a tumor microenvironment. We believe that highly selective MMP-2-responsive fluorescent nanoprobes have broad impacts on biomedical applications including molecular imaging and labeling for tumor detection.</P>

어린이집 영유아 대상 체험활동을 통한 바다의 환경오염에 대한 이해 및 식습관 개선효과: “바다 이야기” 활동

강유진(Yu Jin Kang),김나은(Na Eun Kim),동다흰(Da Huin Dong),유아리(A Ri Yoo),이혜진(Hye Jin Lee),노정옥(Jeong Ok Rho) 한국생활과학회 2023 한국생활과학회 학술대회 Vol.2023 No.5

Cardiac-derived stem cell engineered with constitutively active HIF-1a gene enhances blood perfusion of hindlimb ischemia

Xing Pei,Jiyoung Shin,김희정,Nana Wang,Chaewon Seo,Miyun Yoon,Xiongwen Chen,Jianqing Gao,Victor C. Yang,Huining He,Seungjin Lee 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.105 No.-

Stem cell-based therapeutic approach provides a possible treatment for critical limb ischemia (CLI) byinducing revascularization and regenerating ischemic tissue. However, the clinical benefit is modestdue to low cell survival and limited efficacy after transplantation. Cardiac-derived stem cells (CSCs) mightbe a novel cell source for CLI treatment owing to their superb endothelial differentiation potential andangiogenic paracrine functions. In this study, the angiogenic ability of CSCs was maximized by geneticengineering with constitutively active form of hypoxia-inducible factor-1a (CA-HIF-1a), resistant tooxygen-dependent degradation. CSCs transfected with CA-HIF-1a (CA-HIF-CSCs) promoted supplementaryexpression of proangiogenic factors including VEGF, bFGF, Ang-1 and PDGF-B, along with enhancedangiogenic function including migratory effect, tube formation and endothelial differentiation potential. In the mouse CLI model, CA-HIF-CSCs transplanted into the ischemic region using fibrin gel as cell deliveryvehicle, improved blood perfusion and limb functional recovery with minimal incidence of foot necrosisand limb loss by promoting new vessel formation. Histological evidence further confirmed that CAHIF-CSC/gel treatment markedly alleviated muscle degeneration and fibrosis. CSCs genetically engineeredwith constitutively active HIF-1a provide a novel therapeutic modality in CLI combining stem cell andgene therapy.