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Agar-Gel Immunodiffusion Test를 이용한 돼지흉막폐염(Swine Pleuropneumoniae)의 진단에 관한 연구
심항섭,장경수,조용성,전무형 충남대학교 수의과대학 동물의과학연구소 1994 動物醫科學硏究誌 Vol.2 No.-
To establish an effective diagnostic measure for detection of the antibodies against Actinobacillus pleuropneumoniae, that is the primary agent of swine pleuropneumoniae, the methods for micro-agglutination test(MAT) and agar-gel immunodiffusion test(AGID) were improved and standarized and the comparative studies were carried out. The results obtained through the experiments were summarized as follows. 1. The rabbit hyperimmune sera to reference serotypes 1 to 6 were cross-tested with MAT and AGID. The range of antibody titers on MAT was 80 to 1280, showing the cross-reaction in serotypes 3, 4, 5 and 6. In AGID, the range of antigen titers was 8 to 32, and cross-reaction was observed in serotype 5. The optimal concentration of antigen MAT were 1.25㎎/㎖ 2. In AGID, the most promising antigen and the buffer for agar-gel were EDTA-treated antigen and 0.05M tris buffer(pH 7.2), respectively. 3. By the tests for 200 swine sera, it was found that the accumulative frequency of positive reaction for the 6 serotypes were 240 in MAT and 163 in AGID. 4. when compared the results of AGID with those of MAT for 200 swine sera, all sera with MAT titier under 20 were negative in AGID. Of the sera with MAT titer 40 and 80, 24.7% and 93.9% were positive in AGID, respectively. All sera with MAT titer over 160 showed positive in AGID. 5. In conclusion, the established MAT showed high sensitivity but low specificity, whereas AGID revealed low sensitivity but high specificity. The established AGID test was considered very efficient for detection of antibodies and serotyping of swine sera.
Monocrotophos poisoning in wild mallards (Anas platyrhynchos)
Shim, Hang-Sub,Kim, Hae-Sung,Woo, Jong-Tae,Kim, In-Seop,Jung, Hae-Sun,Song, Eun-Ah,Bark, Jun-Jo The Korean Society of Veterinary Service 2007 韓國家畜衛生學會誌 Vol.30 No.4
The toxicity of organophosphate arises from disruption of the nervous system due to the inhibition of cholinesterase enzymes, leading to death. Six dead mallards were found at Ansung where is one of the most popular wintering sites for migratory birds in Korea, and requested for diagnosis to Gyeonggi Veterinary Service on January of 2007. Some examinations including polymerase chain reaction (PCR) could not find any evidence of specific disease condition. However, the contents of gastrointestinal tracts of the birds contained residues of monocrotophos ranged from 31.3ppm to 294.3ppm by gas chromatography and mass spectrometry. It can be supposed that monocroptophos was responsible for the death of mallards by this results.
Monocrotophos poisoning in wild mallards(Anas platyrhynchos)
( Hang Sub Shim ),( Hae Sung Kim ),( Jong Tae Woo ),( In Seop Kim ),( Hae Sun Jung ),( Eun Ah Song ),( Jun Jo Bark ) 한국가축위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.4
The toxicity of organophosphate arises from disruption of the nervous system due to the inhibition of cholinesterase enzymes, leading to death. Six dead mallards were found at Ansung where is one of the most popular wintering sites for migratory birds in Korea, and requested for diagnosis to Gyeonggi Veterinary Service on January of 2007. Some examinations including polymerase chain reaction(PCR) could not find any evidence of specific disease condition. However, the contents of gastrointestinal tracts of the birds contained residues of monocrotophos ranged from 31.3ppm to 294.3ppm by gas chromato-graphy and mass spectrometry. It can be supposed that monocroptophos was responsible for the death of mallards by this results,
돼지에서 Actinobacillus pleuropneumoniae의 혈청학적 진단법에 대한 비교연구
심항섭 ( Hang Sub Shim ),우종태 ( Jong Tae Woo ),조중현 ( Jung Huin Cho ),전무형 ( Moo Hyung Jun ) 한국가축위생학회 1994 韓國家畜衛生學會誌 Vol.17 No.2
To establish an effective diagnostic measure for detection of the antibodies against Actinobacillus pleuropneumoniae, the methods for tube agglutination test(TAT), plate agglutination test(PAT), micro-agglutination test(MAT) and agar-gel immunodiffusion test(ID) were improved and standarized, and the comparative studies were carried out. The results obtained through the experiments were summarized as follows. 1. The rabbit hyperimmune sera to reference serotypes 1 to 6 were cross-tested with TAT, PAT, MAT and ID. In the homologous systems, the range of antibody titers in TAT was 80 to 640, showing the cross-reaction in serotypes 3, 4, 5 and 6. The range of antibody titers in PAT was 4 to 64, showing the cross-reaction in serotypes 3, 4, 5 and 6. In ID, the range of antigen titers was 8 to 32, and cross-reaction was observed in serotype 5. 2. The optimal concentration of antigen in PAT and MAT were 100mg/ml and l.25mg /ml respectively. The most sensitive reaction in MAT was observed in 52°C for 18hrs. 3. In ID, the most promising antigen and the buffer for agar-gel were EDTA-treated antigen and 0.05M tris buffer(pH 7.2), respectively. 4. By the tests for 200 swine sera, it was found that the frequency of positive reaction were 203 in TAT, 240 in PAT and 163 in ID. 5. When compared the titers of TAT with those of MAT for 200 swine sera, MAT showed the higher titer than TAT being increased by relative correlation. Int was found that the titer for positive readings were 20 in TAT and 40 in MAT. 6. when compared the results of ID with those of TAT for 200 swine sera, all sera with TAT titer under 10 were negative in ID. Of the sera with TAT titer 20 and 40, 55.1% nd 91.8% were positive in ID, respectively. All sera with TAT titer above 80 were positive in ID. In comparison of ID and MAT, all sera with MAT titer under 20 were negative in ID. Of the sera with MAT titer 40 and 80, 24.7% and 93.9% were positive in ID, respectively. All sera with MAT titer over 160 showed positive in ID. 7. In conclusion, the established MAT showed high sensitivity but low specificity, wherease ID revealed low sensitivity but high specificity.
Latex 응집반응 및 polymerase chain reaction을 이용한 돼지톡소플라즈마병 감염실태 조사
심항섭 ( Hang Sub Shim ),최경묵 ( Gyeong Muk Choi ),전오숙 ( Oh Sook Jeon ),이수진 ( Su Jin Lee ),우종태 ( Jong Tae Woo ),노기완 ( Ki Woan Ro ) 한국가축위생학회 2008 韓國家畜衛生學會誌 Vol.31 No.1
Between March and October 2007, a total of 516 blood samples from pigs in the Gyeonggi province were examined for seroprevalence against toxoplasmosis by latex agglutination test (LAT) and the detection of antigenic particles among seropositive samples by PCR. In the LAT, 118 (22.8%) were positive, and the unadjusted percentage of seroprevalence rates of breeding and fattening pigs were significant difference. Positive rate (14.1%) in the breeding pigs was much lower than that (27.8%) of the fattening pigs (p<0.001, Pearson`s Chi-square test). The antibody detection rate of sows was lower than fattening pigs, i.e., 15.8% (25/158) and 26% (93/358), respectively (P=0.011, Pearson`s Chi-square test). Among 118 seropositive samples by LAT, 68 (57.6%) were positive in PCR for the detection of the toxoplasma specific-DNA. There was a statistical difference in the positive PCR reaction between the raising pigs(63/93 67.7%) and sows (5/25, 20%) (P<0.01).
효소면역법을 이용한 Brucella abortus 항체 검출에 관한 연구
심항섭 ( Hang Sub Shim ),국정희 ( Jung Hee Kook ),정봉수 ( Bong Su Chung ),고태오 ( Tae Oh Ko ),조중현 ( Jung Hyun Cho ),박유순 ( Yu Soon Park ) 한국가축위생학회 1998 韓國家畜衛生學會誌 Vol.21 No.2
In order to establish a rapid, sensitive and specific diagnostic method for detection of antibody to Brucella abortus, a enzyme-linked immunosorbent assay(ELISA) was adapted. The diagnostic efficacy of the established ELISA was compared with that of the standard tube agglutination test for B abortus. 1. It was found that the optimal concentration of antigen for this ELISA was 51ug/m1, the optimal dilution of conjugate was 1:2000, and the optimal dilution of serum was 1:200, respectively. 2. Cut off value in this ELISA was 1,102 that was determined by mean absorbance(at 492nm) of tube agglutination test negative serum added with the triple value of the standared devation. 3. The relationship between the tube agglutination test and ELISA was showen high corresponding rate with sensitivity(96.3%) and specificity(98.1%). 4. The efficacy of the ELISA for detection of B abortus antibody was compared with tube agglutination test in brucellosis outbreak farm. The sensivity of ELSIA was higher than tube agglutination test.
경기도에서 발생하는 유우부루셀라병에 관한 연구: 1. 감염우의 역학조사 및 분리균의 특성에 관하여
심항섭 ( Hang Sub Shim ),고태오 ( Tae Oh Ko ),유성종 ( Sung Jong Yoo ),우종태 ( Jong Tae Woo ),박병옥 ( Byoung Ok Park ),김성열 ( Soung Yeol Kim ),박유순 ( Yoo Soon Park ) 한국가축위생학회 1996 韓國家畜衛生學會誌 Vol.19 No.3
The survey was performed to provied details about the pattern of bovine brucellosis occurred in Kyunggi province area. The results obtained through the investigations were summarized as follows. Five hundred seventy-three cattle of bovine brucella reactor were occurred in 14 districts among 31 districts in Kyunggi province in 1989-1995. Among them, 370 cattle(64.5 %) were bred in Eastern area(Ichon, Yeju, Kwangju) and 153 cattle(26.7%) in Southern area(Yongin, Ansung, Peungtack). And the number of farms occurred by bovine brucellosis was 110 ones. When we investigated the occurrance frequency for the 110 farms, the ratio of farms which was brocken out just one time was 67.3% and more than twice was 32.7%. In 573 cattle, 271 cattle were reoccurred in farms which had broken out the bovine brucellosis more than one time. And this survey said the interval of reoccurrance was like this; within a month 50.2%, within two month 19.2%, within four month 7.4%, within six month 7.4%, within an year 15.1%, within 2 year 7.0%. Brucella abortus was isolated from 38 cattle of the 61 ones cattles, and in type all isolated belong to biotype 1.
Enzyme-Linked Immunosorbent Assay(ELISA)를 이용한 혈청 및 원유 중의 Mycobacterium bovis 항체 검출에 관한 연구
심항섭 ( Hang Sub Shim ),국정희 ( Jung Hee Kook ),박병옥 ( Byoung Ok Park ),김성열 ( Sung Youl Kim ),박유순 ( Yu Soon Park ) 한국가축위생학회 1997 韓國家畜衛生學會誌 Vol.20 No.2
In order to supplement a diagnostic method for detection of infectious cattle to bovine tuberculosis, performed ELISA for detection of antibody to M bovis in serum and milk. The diagnostic efficacy of the established ELISA was compared with test of the tuberculin skin test for bovine tuberculosis. The positive corresponding rate of serum ELISA and tuberculin skin test showed 84.3%, milk ELISA and tuberculin skin test showed 75.0%, milk ELISA and serum ELISA showed 75.0% respectively. Comparison of the serum and milk to tuberculin antibody concentration in tuberculin positive cattle, the milk contained 1/100-1/150 concentration compared serum tuberculin concentration. The established ELISA was considered efficient for detection of antibodies to M bovis in serum and milk.
심항섭 ( Hang Sub Shim ),국정희 ( Jung Hee Kook ),황영옥 ( Young Ok Hwang ),정봉수 ( Bong Su Jung ),김학열 ( Hak Yeol Kim ),이모란 ( Mo Ran Lee ),유성종 ( Sung Jong Yoo ),강순근 ( Sun Kun Kang ),임경애 ( Kyung Ae Lim ),고태오 ( Ta 한국가축위생학회 1998 韓國家畜衛生學會誌 Vol.21 No.3
Since bovine leukosis caused considerable economic loss to the dairy industry, seroepidemiological survey on bovine leukosis was carried out for the dairy herds in Kyunggi province. 1. When compared the results of immunodifussion test with those of enzyme-linked immunosor-bent assay(ELISA) for 94 dairy herds sera, the relationship between the immunodifussion test and ELISA were showen high corresponding rate with sensitivity(97.5%) and specificity(92.6%). 2. In immunodiffusion test for bovine leukosis virus (BLV) antibody in 570 dairy cattle from 30 herds, mean positive rate for BLV antibody was 28.2 %. The positive rate by districts were 16.5% in central, 35.4% in east, 17.3% in west, 29.1% in south, 31.6% in north, 43.7% in northeast. 3. When the results of serological studies was analyzed by age groups, the number of positive was increased gradually with the advanced in age of herds. The highest positive rate was found in the age over 6 years. 4. Of 30 dairy herds examined, 5 herds(16.7%) have no reactions against BLV antigen while 15 herds (50%) showed the range of 1~5 positive cattle and 5 herds(16.7%), the rang of over 11 positive cattle.
Yang, Su-Jeong,Shim, Hang-Sub,Woo, Jong-Tae,Kim, Hye-Sung,Lee, Sung-Sik The Korean Society of Veterinary Service 2007 韓國家畜衛生學會誌 Vol.30 No.2
Bovine brucellosis has occurred for years in Gyeonggi province under the national test and slaughter scheme. The serum agglutination test (SAT) is a diagnostic tool to confirm the disease despite the argument on its specificity. We selected 8 farms where only one or two individuals were diagnosed as brucellosis through SAT at the primary regular herd check and isolated the causative organism and characterized the species by species-specific PCR. The pathogen isolation was successful in 6 farms out of 8 farms by microbiological culture, showing the successful rate of 75%. The isolation rate of the causative organism represents 70% from supra-mammary lymph node and 60% from uterine tissues. They were characterized as Brucella abortus biovar 1 after biotyping by PCR, showing the fragment of 498 bp. Five of 8 farms were diagnosed as brucellosis two to four times more over the intervals of two or three months. Here in this study we briefly showed the correlation of the sporadic outbreak of brucellosis tested by SAT and the isolation of the causative organism. Moreover one or two reactors against brucellosis among considerable size of herd may indicate that SAT failed to detect potentially infected individuals in the incubation stage or chronic phase of the disease.