Recombinant Kluyveromyces lactis expressing highly pathogenic porcine reproductive and respiratory syndrome virus GP5 elicits mucosal and cell-mediated immune responses in mice

Haiyan Zhao,Yalan Wang,Zhitao Ma,Yongqiang Wang,Wen-hai Feng 대한수의학회 2014 Journal of Veterinary Science Vol.15 No.2

Currently, killed-virus and modified-live porcinereproductive and respiratory syndrome virus (PRRSV)vaccines are used to control porcine reproductive andrespiratory syndrome. However, both types of vaccines haveinherent drawbacks; accordingly, the development of novelPRRSV vaccines is urgently needed. Previous studies havesuggested that yeast possesses adjuvant activities, and it hasbeen used as an expression vehicle to elicit immune responsesto foreign antigens. In this report, recombinantKluyveromyces lactis expressing GP5 of HP-PRRSV(Yeast-GP5) was generated and immune responses to thisconstruct were analyzed in mice. Intestinal mucosalPRRSV-specific sIgA antibody and higher levels of IFN-γ inspleen CD4+ and CD8+ T cells were induced by oraladministration of Yeast-GP5. Additionally, Yeast-GP5administered subcutaneously evoked vigorous cell-mediatedimmunity, and PRRSV-specific lymphocyte proliferationand IFN-γ secretion were detected in the splenocytes of mice. These results suggest that Yeast-GP5 has the potential foruse as a vaccine for PRRSV in the future.

Research on the Forming Mechanism of Group Doctor-Patient Conflicts in China Based on the Complex Social Networks

Haiyan Zhao,Yuanqing Lu,Fangxing Ye 보안공학연구지원센터 2014 International Journal of Future Generation Communi Vol.7 No.1

Needle Deflection during Insertion into Soft Tissue Based on Virtual Spring Model

Haiyan Du,Yongde Zhang,Jingang Jiang,Yanjiang Zhao 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.1

Needle insertion is the most common procedure of minimally invasive interventions. During insertion into a soft tissue, the needle with bevel tip can deflect due to the asymmetric forces acting on the tip of the needle. In this paper, a mechanics-based model is developed to predict the needle deflection. In the model, the needle is considered as a cantilever beam supported by a series of nonlinear springs each of which has stiffness different from each other. The value of stiffness can be calculated by cutting force acting on the needle tip. Based on the model and the analysis of cutting force and friction force, Rayleigh-Ritz method is used to estimate the amount of needle deflection. Experiment shows that the simulation model can accurately predict the deflection of the bevel-tipped needle.

CO2 Adsorption on the B12N12 Nanocage Encapsulated with Alkali Metals: A Density Functional Study

Haiyan Zhu,Qiyan Zhang,Qinfu Zhao,He Zhao,Yifan Feng,Bingbing Suo,Huixian Han,Qi Song,Yawei Li,Wenli Zou,Haiyan Zhu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.3

Density functional theory (DFT) calculations have been carried out to study the capacity of the B12N12 nanocage encapsulated with alkali metals (Li, Na, K) for the CO2 adsorption and activation. It is found that after encapsulating alkali metals, the alkali metal atoms are closer to one side of clusters instead of exactly lying at the center, and a considerable charge transfers from the inner alkali metal atoms to the B12N12 cage. Besides, the HOMO–LUMO gap (HLG) values of Li@B12N12, Na@B12N12 and K@B12N12 are decreased to about 6 eV, being much smaller than that of the pristine B12N12. Although the geometry structure parameters and the energy differences of M06-2X are slightly different from the ones of ωB97X-D, some identical results of two kinds of functional can be obtained. CO2 can be adsorbed chemically and physically on majority bonds of all the clusters, except for some bonds with large change in bond length and bond indices. The encapsulation of alkali-metal atoms may enhance the physical and chemical adsorption of CO2 on the surface of the clusters, in which Na@B12N12 and K@B12N12 are the most powerful physical and chemical adsorbent for CO2, respectively.

The Philosophical Thinking of the World University Games

( Haiyan Zhao ),( Song Ma ),( Chunyan Li ) 한국체육학회 2015 국제스포츠과학 학술대회 Vol.2015 No.1

Through studying the teleology, subject theory and methodology of the World University Games, this research topic aims to analyze the current orientation of development, subject of development and mode of development and appeal for setting objectives, grasping the significance of subject and improving the mechanism of competition in modern world university games in order to rebuild a sound image of world university games.

SCAMP5 Plays a Critical Role in Synaptic Vesicle Endocytosis during High Neuronal Activity

Zhao, Haiyan,Kim, Yoonju,Park, Joohyun,Park, Daehun,Lee, Sang-Eun,Chang, Iree,Chang, Sunghoe Society for Neuroscience 2014 The Journal of neuroscience Vol.34 No.30

<P>Secretory carrier membrane protein 5 (SCAMP5), a recently identified candidate gene for autism, is brain specific and highly abundant in synaptic vesicles (SVs), but its function is currently unknown. Here, we found that knockdown (KD) of endogenous SCAMP5 by SCAMP5-specific shRNAs in cultured rat hippocampal neurons resulted in a reduction in total vesicle pool size as well as in recycling pool size, but the recycling/resting pool ratio was significantly increased. SCAMP5 KD slowed endocytosis after stimulation, but impaired it severely during strong stimulation. We also found that KD dramatically lowered the threshold of activity at which SV endocytosis became unable to compensate for the ongoing exocytosis occurring during a stimulus. Reintroducing shRNA-resistant SCAMP5 reversed these endocytic defects. Therefore, our results suggest that SCAMP5 functions during high neuronal activity when a heavy load is imposed on endocytosis. Our data also raise the possibility that the reduction in expression of SCAMP5 in autistic patients may be related to the synaptic dysfunction observed in autism.</P>

Effects of Antioxidant Tempol on Systematic Inflammation and Endothelial Apoptosis in Emphysematous Rats Exposed to Intermittent Hypoxia

Haiyan Zhao,Yaping Zhao,Xin Li,Leiqian Xu,Fangxin Jiang,Wanju Hou,Lixia Dong,Jie Cao 연세대학교의과대학 2018 Yonsei medical journal Vol.59 No.9

Purpose: Obstructive sleep apnea and chronic obstructive pulmonary disease are independent risk factors of cardiovascular disease(CVD), and their coexistence is known as overlap syndrome (OS). Endothelial dysfunction is the initial stage of CVD; however,underlying mechanisms linking OS and CVD are not well understood. The aim of this study was to explore whether OS canlead to more severe inflammation and endothelial apoptosis by promoting endothelial dysfunction, and to assess the interventioneffects of antioxidant tempol. Materials and Methods: Male Wistar rats (n=66) were exposed to normal oxygen [normal control (NC) group], intermittent hypoxia(IH group), cigarette smoke (CH group), as well as cigarette smoke and IH (OS group). Tempol intervention was assessed inOS group treated with tempol (OST group) or NaCl (OSN group). After an 8-week challenge, lung tissues, serum, and fresh bloodwere harvested for analysis of endothelial markers and apoptosis. Results: The levels of intracellular adhesion molecule-1, vascular cellular adhesion molecule-1, and apoptosis in circulating epithelialcells were the highest in OS group and the lowest in NC group. These levels were all greater in IH group than in CH group,and were lower in OST group than in OS and OSN groups (all p<0.001). Conclusion: Synergistic effects of IH with cigarette smoke-induced emphysema produce a greater inflammatory status and endothelialapoptosis. OS-related inflammation and endothelial cell apoptosis may play important roles in promoting cardiovasculardysfunction, and antioxidant tempol could achieve a partial protective effect.

Driving Factors on the Adoption of Low Carbon Production: Evidence from 265 Enterprises Managers in Jiangsu Province of China

Yuanqing Lu,Haiyan Zhao,Fang Huang,Jin Wang 보안공학연구지원센터 2014 International Journal of u- and e- Service, Scienc Vol.7 No.5

It’s the inevitably choice for enterprises to low carbon production, which is the important way to cope with the carbon limitation in the future and to gain competitiveness. Based on factor analysis, present paper developde low carbon production behavior questionnaire and investigated 265 enterprises managers in JiangsuProvince. Results of PLS test shows that: government policies, enterprise development, foreign trade have apparent effects on low carbon production behavior; the role of the public pressure to low carbon production behavior is not found. Through the driving factors analysis, present paper proposed some policy suggestions.

Tetrasodium iminodisuccinate assisted hydrothermal synthesis and upconversion luminescence of NaYF4: Yb3+, Er3+ hexagonal nanoplates

Haiyan Lin,Zikun Wang,Yujiang Wang,Junwei Zhao 한양대학교 세라믹연구소 2020 Journal of Ceramic Processing Research Vol.21 No.6

Yb3+/Er3+ co-doped NaYF4 (NaYF4: Yb3+, Er3+) hexagonal nanoplates were synthesized by a tetrasodium iminodisuccinateassisted hydrothermal synthesis method for the first time. The crystal structure and morphology was investigated by the Xraydiffraction and scanning electron microscope analysis. The upconversion luminescent property of the synthesized NaYF4:Yb3+, Er3+ hexagonal nanoplates was discussed as a function of Yb3+ and Er3+ concentration. Under 980 nm excitation, NaYF4:Yb3+, Er3+ hexagonal nanoplates exhibited strong red emissions near 660 nm and green upconversion emissions at 530 and 550nm corresponding to the intra 4f transitions of Er3+ (4F9/2, 2H11/2, 4S3/2) → Er3+ (4I15/2). The Yb3+ 5 mol% and Er3+ 0.5 mol%co-doped NaYF4 sample exhibited strongly shiny upconversion emission, which was considered to be the optimum dopingconcentration. A possible upconversion mechanism for NaYF4: Yb3+, Er3+ depending was discussed in detail.

Low pH stress responsive transcriptome of seedling roots in wheat (Triticum aestivum L.)

Haiyan Hu,Jie He,Junjie Zhao,Xingqi Ou,Hongmin Li,Zhengang Ru 한국유전학회 2018 Genes & Genomics Vol.40 No.11

Soil acidification is one of major problems limiting crop growth and especially becoming increasingly serious in China owing to excessive use of nitrogen fertilizer. Only the STOP1 of Arabidopsis was identified clearly sensitive to proton rhizotoxicity and the molecular mechanism for proton toxicity tolerance of plants is still poorly understood. The main objective of this study was to investigate the transcriptomic change in plants under the low pH stress. The low pH as a single factor was employed to induce the response of the wheat seedling roots. Wheat cDNA microarray was used to identify differentially expressed genes (DEGs). A total of 1057 DEGs were identified, of which 761 genes were up-regulated and 296 were downregulated. The greater percentage of up-regulated genes involved in developmental processes, immune system processes, multi-organism processes, positive regulation of biological processes and metabolic processes of the biological processes. The more proportion of down-regulation genes belong to the molecular function category including transporter activity, antioxidant activity and molecular transducer activity and to the extracellular region of the cellular components category. Moreover, most genes among 41 genes involved in ion binding, 17 WAKY transcription factor genes and 17 genes related to transport activity were up-regulated. KEGG analysis showed that the jasmonate signal transduction and flavonoid biosynthesis might play important roles in response to the low pH stress in wheat seedling roots. Based on the data, it is can be deduced that WRKY transcription factors might play a critical role in the transcriptional regulation, and the alkalifying of the rhizosphere might be the earliest response process to low pH stress in wheat seedling roots. These results provide a basis to reveal the molecular mechanism of proton toxicity tolerance in plants.