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Shin, Yong Cheol,Song, Su-Jin,Shin, Dong-Myeong,Oh, Jin-Woo,Hong, Suck Won,Choi, Yu Suk,Hyon, Suong-Hyu,Han, Dong-Wook Informa UK (TaylorFrancis) 2018 Applied spectroscopy reviews Vol.53 No.2
<P>Skeletal muscle injuries are extremely common because skeletal muscle is quite frequently used in the human body, and these injuries can cause serious health implications. Currently, grafting and pharmacological therapies are the most common therapeutic methods for treating and repairing the skeletal muscle damages, but both therapeutic methods have significant limitations. Therefore, in recent years, the tissue engineering approaches have attracted much attention in biomedical and bioengineering fields. In particular, up-to-date studies have focused on the novel strategies aimed at promoting and enhancing the regeneration of skeletal muscle tissue by using tissue engineering scaffolds. Although the tissue engineering scaffolds can be readily fabricated with conventional biocompatible materials, such as polymer, ceramic, or metallic materials, the carbon nanomaterials (CNMs) are the most fascinating candidates as a scaffold material due to their favorable biocompatibility and extraordinary physicochemical, electronic, mechanical, and thermal properties. The aim of this review is to summarize some of the recent reports concerning the nanocomposite scaffolds functionalized with CNMs and to highlight promising perspective for the applications of CNMs as skeletal tissue engineering scaffolds. In addition, it is also discussed how the spectroscopic analysis can be employed for analyzing CNMs and nanocomposite scaffolds.</P>
Shin Dong-Jun,Choy Hyon E.,Hong Yeongjin The Microbiological Society of Korea 2005 The journal of microbiology Vol.43 No.3
In eukaryotic cells, various proteins are anchored to the plasma membrane through glycosylphosphatidylinositol (GPI). To study the biosynthetic pathways and modifications of GPI, various mutant cells have been isolated from the cells of Chinese hamster ovaries (CHO) supplemented with several exogenous genes involved in GPI biosynthesis using aerolysin, a toxin secreted from gram-negative bacterium Aeromonas hydrophila. Alpha toxin from Gram-positive bacterium Clostridium septicum is homologous to large lobes (LL) of aerolysin, binds GPI-anchored proteins and possesses a cell-destroying mechanism similar to aerolysin. Here, to determine whether alpha toxins can be used as an isolation tool of GPI-mutants, like aerolysin, CHO cells stably transfected with several exogenous genes involved in GPI biosynthesis were chemically mutagenized and cultured in a medium containing alpha toxins. We isolated six mutants highly resistant to alpha toxins and deficient in GPI biosynthesis. By genetic complementation, we determined that one mutant cell was defective of the second subunit of dolichol phosphate mannose synthase (DPM2) and other five cells were of a putative catalytic subunit of inositol acyltransferase (PIG-W). Therefore, C. septicum alpha toxins are a useful screening probe for the isolation of various GPI-mutant cells.
난소의 생식세포 기원성 종양세포에서의 c-myc, c-H-res 관련 단백 및 표피성장인자에 관한 면역조직학적 연구
현동관,신용칠,염범우 고려대학교 의과대학 1993 고려대 의대 잡지 Vol.30 No.1
The causes of malignant tumor include chemical agents, irradiation, viral infection and others. Among them, oncogenic viral infection clarified the pathogenesis of the neoplasm. Oncogenic virus has viral oncogene which has transforming activity of cells when it is incoorperated into the DNA of the host cells. These DNA of transformed cells also have the activity of transformation of recipient cells and production of infectious virus, in culture. The normal cells may have the DNA showing homologous or similar structure of viral oncogene. These cellular oncogenes may be activated by gene mutation chromosomal translocation, deletion and promotor insertion. In these cases, abnormal oncogene related proteins are produced, such as ras P21 and P62-c-myc proteins. There are many reports that oncogene related proteins are detected in variable carcinomas, sarcomas, and leukemia-lymphomas. However, there are few reports on germ cell tumors of ovary So we stained rasP21 (ras gene related protein), P62-c-myc (c-myc related protein) and EGF antisera on various ovarian germ cell tumors with the method of avidin-biotin peroxidase complex. The results are as followings, 1. The positive reaction of all three antisera against the oncoprotein is the highest in cystic teratoma. 2. In cystic teratomas, the squamous cells and sebaceous glands show the strongest positive reaction and chondroid and glial tissue shows the weakest positive reaction. 3. c-myc antiserum shows the strongest positive reaction in the most tumors comparing with that of other two antisera.
Shin, Yong Cheol,Yang, Won Jun,Lee, Jong Ho,Oh, Jin-Woo,Kim, Tai Wan,Park, Jong-Chul,Hyon, Suong-Hyu,Han, Dong-Wook Dove Medical Press 2014 International journal of nanomedicine Vol.9 No.-
<P>This study concentrates on the development of biodegradable nanofiber membranes with controlled drug release to ensure reduced tissue adhesion and accelerated healing. Nanofibers of poly(lactic-<I>co</I>-glycolic acid) (PLGA) loaded with epigallocatechin-3-<I>O</I>-gallate (EGCG), the most bioactive polyphenolic compound in green tea, were electrospun. The physicochemical and biomechanical properties of EGCG-releasing PLGA (E-PLGA) nanofiber membranes were characterized by atomic force microscopy, EGCG release and degradation profiles, and tensile testing. In vitro antioxidant activity and hemocompatibility were evaluated by measuring scavenged reactive oxygen species levels and activated partial thromboplastin time, respectively. In vivo antiadhesion efficacy was examined on the rat peritonea with a surgical incision. The average fiber diameter of E-PLGA membranes was approximately 300–500 nm, which was almost similar to that of pure PLGA equivalents. E-PLGA membranes showed sustained EGCG release mediated by controlled diffusion and PLGA degradation over 28 days. EGCG did not adversely affect the tensile strength of PLGA membranes, whereas it significantly decreased the elastic modulus and increased the strain at break. E-PLGA membranes were significantly effective in both scavenging reactive oxygen species and extending activated partial thromboplastin time. Macroscopic observation after 1 week of surgical treatment revealed that the antiadhesion efficacy of E-PLGA nanofiber membranes was significantly superior to those of untreated controls and pure PLGA equivalents, which was comparable to that of a commercial tissue-adhesion barrier. In conclusion, the E-PLGA hybrid nanofiber can be exploited to craft strategies for the prevention of postsurgical adhesions.</P>
( Dong Yun Shin ),( Jong Han Lee ),( Hyon Suk Kim ),( Min Geol Lee ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2
Background: Appropriate diagnosis protocols for syphilis disease should be well established. Recently, automated non-treponemal reagin (rapid plasma reagin, RPR) tests are developed and more frequently used than conventional RPR card test. Objectives: To evaluate automated RPR test comparing to conventional RPR card test for its clinical application Methods: A total of 62 patients` serum samples with syphilis and 50 syphilis-negative controls were enrolled for this evaluation. The percent agreement, kappa value, and overall sensitivity and specificity were compared between two RPR tests. Also, seroconversion rate of each RPR test were evaluated. Results: The percent agreement of both RPR tests was 78.6%. Overall sensitivity and specificity of the auto RPR test to TPPA (treponema pallidum particle agglutination assay) was 50% and 64%, respectively, while the same values for conventional RPR card test were 84.13% and 97.96%, respectively. The conventional RPR card test showed overall high positivity than the automated RPR test. The automated RPR test showed a higher seronegative changes (57.1%, 12/21) than the RPR card test (19%, 4/21) after treatment for syphilis (P=0.025). Conclusion: The automated RPR test showed a lower positivity compared to TPPA which shows high positivity during all the stages of syphilis regardless of treatment. However, the automated RPR test may be helpful to monitor the seroconversion responses after treatment for syphilis over a relatively short period of time.
Shin, Dong-Jun,Cho, Duck,Kim, Young Ran,Rhee, Joon Haeng,Choy, Hyon E.,Lee, Je-Jung,Hong, Yeongjin S. Karger AG 2006 Journal of molecular microbiology and biotechnolog Vol.11 No.1
<P>Paroxysmal nocturnal hemoglobinuria (PNH), a hematopoietic stem cell disorder, is caused by the loss of glycosylphosphatidylinositol (GPI)-anchored proteins on the cell membrane. PNH can be simply diagnosed by flow cytometry using monoclonal antibodies against GPI-anchored proteins or fluorescent-tagged aerolysin, a bacterial toxin that binds GPI anchored proteins. <I>Clostridium septicum</I> alpha toxin is homologous to aerolysin and specifically binds GPI-anchored proteins. Previously, we found that an alpha toxin m45 mutant with two amino acid changes, S189C/S238C, lost cytotoxicity but still possessed binding activity for GPI-anchored proteins. To use this mutant toxin as a diagnostic probe in flow cytometry, we constructed the EGFP-AT(m45) expression vector, comprising a S189C/S238C alpha toxin mutant with EGFP and His tags at the N and C termini, respectively. The recombinant EGFP-AT(m45) was easily purified using single-step affinity chromatography against His tag from <I>Escherichia coli</I>. EGFP-AT(m45) bound to CHO and HeLa cells in a similar manner to monoclonal antibodies against GPI-anchored proteins or aerolysin. In whole blood from a PNH patient, GPI-deficient granulocytes could be differentiated by EGFP-AT(m45) using the same procedure as that employed with commercially available monoclonal antibodies. Therefore, nontoxic EGFP-conjugated <I>C. septicum</I> alpha toxin could be used clinically for PNH diagnosis.</P><P>Copyright © 2006 S. Karger AG, Basel</P>