Synergistic killing effect of imatinib and simvastatin on imatinib-resistant chronic myelogenous leukemia cells

Oh, Bora,Kim, Tae Y.,Min, Hyun J.,Kim, Miyoung,Kang, Myung S.,Huh, Ji Y.,Kim, Youngsoo,Lee, Dong S. Lippincott Williams Wilkins, Inc. 2013 Anti-cancer drugs Vol.24 No.1

The antiproliferative effect of simvastatin on tumor cells has been speculated to be by intracellular signal inhibition through 3-hydroxy-3-methylglutaryl acetyl coenzyme A reductase. We examined the killing effect of simvastatin on imatinib-sensitive and resistant chronic myelogenous leukemia (CML) cells (three kinds of CML cell lines representative of each hematopoietic lineage: K562, KCL22, and LAMA84) and T315I and E255K site-directed mutant cells (Ba/F3). The in-vivo effect of simvastatin was determined in K562-xenografted nude mice. Cotreatment with imatinib and simvastatin in imatinib-resistant CML cells showed a synergistic killing effect in K562-R, KCL22-R, LAMA84-R, and E255K mutant cells, but only an additive effect in the T315I mutant cell, although a single treatment of simvastatin strongly inhibited T315I mutant cells. Mechanisms of killing were an induction of apoptosis and cell cycle arrest, through inhibition of tyrosine phosphorylation, and activated STAT5 and STAT3. Simvastatin suppressed the growth of K562-transplanted tumors, and cotreatment with imatinib was more effective in reducing tumor size. Simvastatin also killed primary CD34 cells from patients with CML more efficiently, compared with CD34 CML cells. Our study shows a synergic effect of imatinib and simvastatin both in imatinib-sensitive and imatinib-resistant cells, but more effective synergism in resistant cells. On the basis of these findings, we suggest that a combination of simvastatin and imatinib may be a potential candidate for the treatment of imatinib-resistant CML.

α-Amylase 첨가에 따른 쌀가루 및 팽화미분 아이스크림의 이화학적 특성

오보라 ( Bora Oh ),이영현 ( Young Hyoun Yi ) 한국산업식품공학회 2016 산업 식품공학 Vol.20 No.1

단립종 쌀가루, 장립종 쌀가루, 단립종 팽화미분 및 장립종 팽화미분의 호화도와 α-amylase 첨가에 따른 쌀가루와 팽화미분 아이스크림의 점도, 오버런, 녹아내리는 정도, 수분, 조지방, 총당 그리고 색도를 조사하였다. 호화도는 동일 곡종에서는 팽화로 호화도가 증가하였으며, 동일 처리구에서는 단립종이 장립종 보다 높았다. 점도는 α-amylase 농도가 증가할수록 감소했고 0.0%를 제외한 동일 농도와 곡종에서는 쌀가루가 팽화미분보다 높았으며, 같은 농도와 처리구에서는 단립종이 높았다. 오버런은 0.2%가 가장 높았고 같은 농도와 처리구에서는 단립종이 높았다. 녹아내리는 정도는 믹스 점도가 낮아질수록 많이 녹아내렸고, 팽화미분이 쌀가루보다 많았다. 수분은 효소함량에 따라 유의적 차이가 없었으며, 팽화유무에 상관없이 단립종이 높거나 같았다. 조지방은 농도, 곡종 및 팽화유무와 상관없이 유의적 차이가 없었다. 총당 함량은 α-amylase 농도가 증가할수록 높아졌고 동일 농도에서는 팽화미분이 높은 값을 보였다. 색도는 효소 농도가 짙어질수록 “L”값과 “b”값은 증가하였고 “a”값은 감소하였다. 동일한 농도와 곡종에서는 팽화에 의해 “b”값은 감소하였다(p<0.05). The viscosity, overrun, melting-down, moisture, crude fat, total sugar, and color of rice powder and puffed rice powder ice cream, following the addition of α-amylase, were investigated. For identical grain types, the gelatinization degree increased with puffing, and within the same treatment, the short grain was higher than the long grain. Viscosity dropped with increasing α-amylase at the same concentration and grain type, excluding 0.0%, the rice powder was higher than the puffed one, and for the same concentration and treatment, the short grain was higher. The overrun was highest at 0.2%, and for the same concentration and treatment, the short grain exhibited higher overrun. Higher melting-down was observed in puffed and lower viscosity ice cream mix. No significant difference was found in moisture with enzyme concentration. Regardless of puffing, the short tended to have a higher moisture. No difference was noticed in crude fat by concentration, grain type, or puffing. The total sugar was higher with increasing α-amylase; at the same concentration, puffed tended to be higher. The hunter “L” and “b” increased with α-amylase, while the “a” value dropped. At the same concentration and grain type, the “b” values decreased with puffing (p<0.05).

대학생들의 수업 적응도에 대한 잠재전이분석

오종현(Oh, JongHyun),오보라(Oh, BoRa),김우성(Kim, WooSung) 학습자중심교과교육학회 2017 학습자중심교과교육연구 Vol.17 No.17

The purpose of this study is to investigate the transitions of class adjustment for university students over time. Although there are previous studies related to class adjustment, there are little studies exploring longitudinal changes in class adjustment. This study carried out questionnaire survey on class adjustment to university in March and May 2016 for university students in Gyeongsangbuk-do area. The data of 262 students who answered both the first and second questionnaires were analyzed. As a result of the analysis of the latent profiles, the class adjustment’s profiles were classified into Adjustment_difficulty , Adjustment_medium , and Adjustment_high The results of latent transition analysis on the class adjustment shows that the students had difficulty adjusting to the class over time. In order to determine whether individual characteristics were predictive of class adjustment behavior profiles at Time 1 or transitions in class adjustment from Time 1 to Time 2, gender and high school’s tracks(liberal arts vs science) were incorporated into the Latent Transition Analysis model. The result shows that female students are 3.236 times more likely to be in ‘ Adjustment_difficulty ’ state than male students. The level of academic achievement also differs between latent states on class adjustment. Lastly , the implications and limitations of this stud y were discussed. 본 논문에서는 잠재 전이 분석을 통하여 대학생의 수업 적응도 변화를 탐색한다. 지금까지 학업 적응과 관련된 다양한 연구들이 선행되었으나, 개별 수업에서의 적응의 변화를 종단적으로 탐색한 연구는 미비하였다. 표본은 경북 지역의 대학생을 대상으로 하였으며, 2016년도 3월과 5월, 2회에 걸쳐 수업 적응도 설문 조사를 실시하여 1·2차 설문조사에 모두 응답한 학생 262명의 자료를 수집, 분석하였다. 분석 결과, 대학생들의 수업 적응도는 ‘적응_어려움’, ‘적응_중간’, ‘적응_높음’이라는 3개의 잠재 프로파일로 분류됨을 확인하였으며, 학생들은 학기 초 보다는 학기 말에 수업에 적응하는 것을 더 어려워함을 관찰하였다. 잠재전이모형에 성별과 고교계열을 공변수로 투입하여 분석한 결과, 여학생이 남학생에 비해 수업적응도 1차에서 ‘적응_어려움’ 잠재상태에 속할 확률이 3.235배 더 높았다. 또한. 수업 적응도 2차 잠재상태에 따라, 학업 성취의 정도가 차이가 있음을 확인하였다.

Actinomadurol, an Antibacterial Norditerpenoid from a Rare Actinomycete, <i>Actinomadura</i> sp. KC 191

Shin, Bora,Kim, Byung-Yong,Cho, Eunji,Oh, Ki-Bong,Shin, Jongheon,Goodfellow, Michael,Oh, Dong-Chan American Chemical Society and American Society of 2016 Journal of natural products Vol.79 No.7

<P>A new secondary metabolite, actinomadurol (1), was isolated along with the known compound JBIR-65 (2) from a rare actinomycete, Actinomadura strain KC 191. The structure of 1 was established as a rare member of the bacterial C-19 norditerpenoid class by NMR data and ECD calculations. The absolute configuration of 2, which was previously reported without stereochemical analysis, was determined by using the modified Mosher's method and ECD calculations. Actinomadurol (1) exhibited potent antibacterial activity against pathogenic strains, such as Staphylococcus aureus, Kocuria rhizophila, and Proteus hauseri (MIC = 0.39-0.78 mu g/mL), whereas JBIR-65 (2) showed no antibacterial activity.</P>

Clinical and Biological Implications of Cell Cycle-Related Gene Fusions and Mutations in Resectable Hepatocellu-lar Carcinomas

( Jihyun An ),( Ji-hye Oh ),( Wonkyung Kim ),( Bora Oh ),( Yoo-jin Oh ),( Chang Ock Sung ),( Ju Hyun Shim ) 대한간학회 2021 춘·추계 학술대회 (KASL) Vol.2021 No.1

Immunotherapy Biomarker-Based Classification and Its Potential Clinical Usefulness in Hepatocellular Carcinoma

( Jihyun An ),( Ji-hye Oh ),( Wonkyung Kim ),( Bora Oh ),( Yoo-jin Oh ),( Chang Ock Sung ),( Ju Hyun Shim ) 대한간학회 2021 춘·추계 학술대회 (KASL) Vol.2021 No.1

Concealment, depression and poor quality of life in patients with congenital facial anomalies

Lim, So-Young;Lee, Dongsoo;Oh, Kap Sung;Nam, Bora;Bang, Sa-lk;Mun, Goo-Hyun; Pyon, Jai-Kyong;Kim, Ji-Hae;Yoon, Se Chang;Song, Hyo-Seok;Jeon, Hong Jin Sookmyung Women's University Research Institute of 2011 여성과 건강 Vol.6 No.2

Objectives: Studies have shown that patients with congenital facial anomalies are vulnerable to depression. In addition, concealment of facial anomalies in an effort to mask handicaps is common, and these patients also often have difficulties with interpersonal rela¬tionships and in social situations. Despite this, no previous study has investigated the associa¬tion between concealment of facial anomalies and depression, and a patient’s quality of life. Methods: A group of 65 patients, who had been scheduled for plastic surgery, completed this study. A total of 50 patients who had congenital facial anomalies, some of whom concealed their facial anomalies (N = 22), and some whom didn’t (N = 28), as well as 15 patient controls were interviewed and subsequently administered the Beck Depression Inventory-ll (BDI-II), the Structured Clinical Interview for DSM-IV (SCID), the Millon Behavioral Medicine Diagnostic (MBMD) and the WHO Quality of Life (WHOQOL). Results: Among patients with congenital facial anomalies, those who concealed their anom¬alies exhibited a significantly higher level of depression and anxiety; higher rates of self¬ accusation, dissatisfaction, hypochondria, weight loss and antisocial personality traits; and a lower quality of life than those who did not conceal their anomalies. To the contrary, no significant differences were found with respect to depression, anxiety and quality of life between the congenital facial anomaly group and controls. Further, the concealment of facial anomalies was a significant predictor for lifetime major depressive disorder (odds ratio (OR) = 7.1, 95% confidence interval (Cl) 1.4-37.3), after adjusting for age, gender and microtia. Conclusion: Facial concealment is a significant predictor of depression and poor quality of life in patients with congenital facial anomalies.

Prognostic Molecular Signatures and S100P Expression in Resectable Hepatocellular Carcinoma

( Jihyun An ),( Hee Sang Hwang ),( Hyo Jeong Kang ),( Bora Oh ),( Yoo-Jin Oh ),( Ji-hye Oh ),( Wonkyung Kim ),( Deokhoon Kim ),( Chang Ohk Sung ),( Ju Hyun Shim ),( Eunsil Yu ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1

Aims: Although hepatocellular carcinoma (HCC) is often recurred in patients undergoing curative hepatectomy, there are no faithful biomarkers to predict this undesirable events. Recent RNA-based efforts have developed valuable genetic markers or signatures prognostic of cancer outcomes. We aimed to discover molecular predictors of recurrence after HCC resection and to unveil the geno-molecular structure of the resected tumors in a series of Korean patients. Methods: Based on transcriptomic and genomic data of 206 HCC samples surgically resected at Asan Medical Center, we performed differential gene expression analysis to find quantitative markers associated with early recurrence; and used unsupervised clustering method to classify molecular subtypes. Public RNA-sequencing datasets from Japan (RIKEN) and China (GSE14520) were also used to validate original findings. Results: The results of differential gene expression analysis showed that S100P was identified as the highest-ranked overexpressed gene in HCCs with early recurrence within 2 years after surgery. This trend was also reproduced in immunohistochemical studies of the original and independent AMC cohorts. On multivariable competing risks modeling, S100P expression independently predicted HCC-specific mortality (adjusted hazard ratio, 1.09; P<0.05). Validation in the GSE14520 cohort and in vitro experiments confirmed the prognostic value of S100P for HCC recurrence. The c-statics of the S100P mRNA for predicting early recurrence confirmed that it had prognostic utility better than that of serum alpha-fetoprotein. We also identified five discrete molecular subtypes of HCC: the subtype with stem cell features (‘AMC-C4’) was associated with the worst prognosis both in our series and another two public datasets. S100P was most significantly upregulated in the subgroup C4 (P<0.05). Conclusions: We discovered a promising prognostic biomolecule, S100P, associated with early recurrence after HCC resection, and verified geno-molecular architecture of tumors affecting clinical outcomes particularly in Asian patients. These new insights into molecular mediators would help to tailor care for affected Asians.

Impaired autophagic flux in the uterine mesenchyme leads to vascular muscle relaxation and increased vascular permeability

Bora Lee,Hyejin Shin,Ji-Eun Oh,Jin-Hyun Jun,Seok-Ho Hong,Haengseok Song,Hyunjung Jade Lim 한국실험동물학회 2018 한국실험동물학회 학술발표대회 논문집 Vol.2018 No.7

Accuracy of Three Digitization Methods for the Dental Arch with Various Tooth Preparation Designs: An In Vitro Study : Assessment of Accuracy of Digitization Methods

Oh, Kyung Chul,Lee, Bora,Park, Young-Bum,Moon, Hong Seok Wiley (Blackwell Publishing) 2019 Journal of prosthodontics Vol.28 No.2