桂枝가 Angiogenesis의 抑制機轉에 미치는 영향

安圭錫,崔昇勳,姜玧熙 대한동의병리학회 1999 동의생리병리학회지 Vol.13 No.2

桂枝의 apoptosis 유도와 angiogenesis 억제 기전을 실험적으로 규명하고자 ECV304 cell line 과 ECVPAR cell line을 이용하여 실험을 시행하여 다음과 같은 결과를 얻었다. 첫째, 세포부착 능력의 경우, 桂枝 煎湯液은 EVC304 cell line 과 ECVPAR cell line에서 5㎍/㎖부터 강한 저애효과를 보였다. 둘째, 桂枝 煎湯液은 ECV304 cell line서 GO/G1 arrest peak를 유발시켰다. 셋째, 桂枝 煎湯液은 ECV304 cell line에서 collagen coated plate에서 부착저지 효과를 나타냈다. 넷째, 桂枝 煎湯液은 ECV304 cell line에서 matrigel을 coating한 plate에서 lumen형성을 저애하였다. 다섯째, 桂枝 煎湯液은 ECV304 cell line 과 ECVPAR cell line에 대한 LFA-1 및 ELAM-1의 발현을 저애하였다. 여섯째, 桂枝 煎湯液은 ECV304 cell line 과 ECVPAR cell line에서 MMP-9과 uPA의 발현을 저애하였다. 일곱째, 桂枝 煎湯液은 ECV304 cell line 과 ECVPAR cell line에서 integrin αvβ3의 발현을 저애하였다. 이상의 실험결과로 보아 桂枝이 세포의 apoptosis를 유도하고 angiogenesis를 억제하는 효과가 있음을 알 수 있었으며, 또한 活血之劑의 angiogenesis 억제효과에 대한 가능성을 제시할 수 있을 것으로 사료된다. This experimental study was carried out to evaluate the effects of Cinnamomi Ramulus(CR) on angiogenic inhibition mechanism. In order to investigate the effects of Cinnamomi Ramulus on angiogenic inhibition mechanism evaluate cell survival rate by MTT assay, cell adhesive inhibition effect, DNA fragmantaion analysis, Nuclear Condensation assay, FACScan analysis, Angiogenesis inhibition assay, lmmunocytochemistry analysis, RT-PCR for mRNA expression, Western Blot analysis. The results were summurized as follows : 1. The cell adhesive inhibition ability was strong from 5㎍/㎖. 2. The GO/G1 arrest peak was existed on ECV304 cell-line. 3. The cells on Collagen plate were inhibition of proliferation and inducement of apoptosis by CR water extract. 4. lumen formation was inhibited by CR water extract. 5. LFA-1 and ELAM-1's expression were inhibited by CR water extract. they are commenly participation on inflammation and tumor regeneration. 6. The expression of MMP-9 and uPA were inhibited by CR water extract. 7. The expression of integrin αvβ3 was inhibited by CR water extract. 8. The expression of intracellular molecule were successively inhibited by CR water extract therefore the proliferation of ECV304 cell line was stopped and apoptosis was induced. According to the results Cinnamomi Ramulus showed to be a key antagonist of intergrin αvβ3, and which seems to be apoptosis by p53 through Flow cytometry. This report demonstrated that expression of MMP-9 and uPA blocked to under angiogenesis model. Thus, we suggested that Cinnamomi Ramulus block angiongenesis by inducing unscheduled apoptosis in ECV304 and ECVPAR cell lines and another oriental herbal medicine that treat Blood-stasis type also have angiogenic inhibition effects.

鬱金이 Angiogenesis의 抑制機轉에 미치는 影響

崔昇勳,安圭錫,成熙根 대한동의병리학회 1999 동의생리병리학회지 Vol.13 No.2

鬱金이 ECV304 cell line 과 ECVPAR cell line에서의 angiogenesis 억제기전에 미치는 영향을 알아보기위하여 실험한 결과 다음과 같은 결과를 얻었다. 첫째, 세포 독성은 ECVPAR cell line에서 10㎍/ml였다. 둘째, 세포부착능력은 ECV304 cell line 과 ECVPAR cell line에서 5㎍/ml부터 강한 저해효과를 보였다. 셋째, ECV304 cell에서 Go/G1 arrest peak가 존재 하였다. 넷째, ECV304 cell line과 ECVPAR cell line에서 Collagen plate에서의 증식억제 및 apoptosis를 유도하였다. 다섯째, ECV304 cell line에서 angiogenesis의 lumen형성을 저해함을 알 수 있었다. 여섯째, ECV304 cell line 과 ECVPAR cell line에서 inflammation 및 tumor regeneration에 공통적으로 관여하는 LFA-1 및 ELAM-1의 발현과 기능을 저해함을 알 수 있었다. 일곱째, ECV304 cell line 과 ECVPAR cell line에서 MMP-9과 uPA의 발현이 억제됨을 알 수 있었다. 여덟째, ECV304 cell line 과 ECVPAR cell line에서 integrin ανβ3의 발현이 억제됨을 알 수 있었다. 아홉째, intracellular molecule의 발현에도 연속적으로 저해하므로써 ECV304 cell line 과 ECVPAR cell line의 증식이 중단되고 apoptosis를 유도 하게 됨을 알 수 있었다. 이상의 실험결과로 보아 鬱金이 ECV304 cell line과 ECVPAR cell line에서 발현되는 intergrin ανβ3의 발현을 억제하여 세포의 apoptosis를 유도하므로써 angiogenesis를 억제하는 효과가 있음을 알 수 있었다. This experimental study was carried out to evaluate the effects of Curcuma on angiogenic inhibition mechanism. In order to investigate the effects of Curcuma on angiogenic inhibition mechanism evaluate cell survival rate by MTT assay, cell adhesive inhibition assay, lmmunocytochemistry analysis, RT-PCR for mRNA expression, Western Blot analysis. The results were summarized as follows: 1. The IC50 was 10㎍/ml to ECV 304 cell 2. The cell adhesive inhibition ability was strong from 5㎍/ml. 3. The G0/G1 arrest peak was existed on ECV304 cell-line. 4. The cells on Collagen plate were inhibition of proliferation and inducement of apoptosis by Cur water extract. 5. Lumen formation was inhibited by Cur water extract. 6. LFA-1 and ELAM-1's expression were inhibited by Cur water extract. they are commonly participation on inflammation and tumor regeneration. 7. The expression of MMP-9 and uPA were inhibited by Cur water extract. 8. The expression of integrin ανβ3 was inhibited by Cur water extract. 9. The expression of intracellular molecule were successively inhibited by Cur water extract therefore the proliferation of ECV304 cell line was stopped and apoptosis was induced. According to the results Curcuma has been shown to be a key antagonist of intergrin ανβ3, and which seems to be apoptosis by P53 through Flow cytometry. This report demonstrated that expression of MMP-9 and uPA blocked to under angiogenesis model. Thus, we suggested that Curcuma water block angiogenesis by inducing unscheduled apoptosis in ECV304 and ECVPAR cell lines and another oriental herbal medicine that treat 뺘-stagnation and Blood-stasis type also have angiogenic inhibition effects.

High Expression of the Urokinase-Type Plasminogen Activator Receptor in SGT Cell Line

최현식,박경주,천윤권,이종헌 대한구강악안면병리학회 2011 대한구강악안면병리학회지 Vol.35 No.6

Urokinase-type plasminogen activator(uPA) bound to urokinase plasminogen activator receptor(uPAR) expression is strongly correlated with the metastatic potential of various tumors by enhancing ECM degradation through plasminogen and matrix metallopreotease activation. But expression of uPA/uPAR in human malignant salivary gland tumors has been rarely reported. The purpose of this study were to investigate mRNA expression and cytologic concentration of uPAR in SGT cell line compared to various cancer cell lines by RT-PCR and ELISA method, and to study migration and adhesion assay. These results would be to apply the pathogenesis and prognosis of malignant salivary gland tumors. All the cell lines(SGT, HN 4, SCC 25, and HeLA) were cultured under DMEM with 10% FBS at 37℃ in a 5% CO2 incubator. We studied a possible association between mRNA expression and cytosolic concentrations of uPAR in SGT cell line compared to various cancer cell lines using RT-PCR and an enzyme-linked immunoassay(ELISA) method. And also cell adhesion and migration assay were done in all the cell lines. In migration assay SGT cell line was about 2.5-4 folds higher than another cell lines. In adhesion assay SGT cell line was about 1.1-2 folds higher than another cell lines. uPAR cytosolic concentrations of SGT cell line was about 3.4-10 folds by ELISA, while mRNA expression was about 2.5-5folds by RT-PCR. Oral Scc cell lines showed the lowest value. uPAR protein and mRNA expression were correlated with migration and adhesion assay. From the aboving results, high cytosolic concentrations and mRNA expression of uPAR were correlated with migration and adhesion assay. It suggested that this might be a specific marker for malignant potential of SGT cell line and would be contributed to pathogenesis and prognosis of human salivary gland adenocarcinoma.

Induction of Apoptosis and Growth-Inhibition by Thymoquinone in ACHN and GP-293 Cell Lines in Comparable with Cis-Platinum

Shahraki, Samira,Mohebbati, Reza,Shafei, Mohammad Naser,Mahmoudi, Mahmoud,Hosseinian, Sara,Parhizgar, Soghra,Yazd, Zohreh Naji Ebrahimi,Heravi, Nazanin Entezari,Abadi, Reza Nejad Shahrokh,Khajavirad, KOREAN PHARMACOPUNCTURE INSTITUTE 2019 Journal of pharmacopuncture Vol.22 No.3

Objective: In the current work, we investigated the cytotoxic and apoptotic effects of Thymoquinone (TQ), an active compound of Nigella sativa (N. sativa) and Cis-platinum, on normal renal epithelial (GP-293) and human renal adenocarcinoma cell lines (ACHN). Methods: GP-293 and ACHN cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% Fetal bovine serum (FBS) and 1% penicillin plus streptomycin antibiotic. The MTT assay was used for cellular viability assessment. Viability of cells was observed using inverted light microscope 24, 48 and 72 h after exposure of the cells to various concentrations of TQ (1, 2.5, 5, 10, 50 and $100{\mu}g/ml$) and Cis-platinum (0.5, 1, 1.5, 2, 3, 6 and $12.5{\mu}g/ml$). Moreover, apoptosis was analyzed with a flow-cytometry method. The untreated cells were considered as control group. Results: Morphological changes such as reduced cell number and increased intercellular distance and reduced cell viability in ACHN and GP-293cell lines were observed in both TQ and Cis- platinum groups; however, Cis-platinum had greater effect on ACHN cell line than GP-293 cell line. In addition, GP-293 cell line was more sensitive to TQ compared to ACHN cell line. Furthermore, TQ and Cis-platinum had apoptotic effects on both ACHN and GP-293 cell lines. Conclusion: Our findings demonstrated that TQ and Cis-platinum had cytotoxic and apoptotic effects on both cell lines, However, GP-293 cell line was more sensitive to TQ. Additionally, Cis-platinum was more effective on ACHN cell line than on GP-293 cell line.

Induction of Apoptosis and Growth-Inhibition by Thymoquinone in ACHN and GP-293 Cell Lines in Comparable with Cis-Platinum

Samira Shahraki,Reza Mohebbati,Mohammad Naser Shafei,Mahmoud Mahmoudi,Sara Hosseinian,Soghra Parhizgar,Zohreh Naji Ebrahimi Yazd,Nazanin Entezari Heravi,Reza Nejad Shahrokh Abadi,Abolfazl Khajavi Rad 대한약침학회 2019 Journal of pharmacopuncture Vol.22 No.3

Objective: In the current work, we investigated the cytotoxic and apoptotic effects of Thymoquinone (TQ), an active compound of Nigella sativa (N. sativa) and Cis-platinum, on normal renal epithelial (GP-293) and human renal adenocarcinoma cell lines (ACHN). Methods: GP-293 and ACHN cell lines were cultured in Dulbecco’s modified Eagle's medium (DMEM) with 10% Fetal bovine serum (FBS) and 1% penicillin plus streptomycin antibiotic. The MTT assay was used for cellular viability assessment. Viability of cells was observed using inverted light microscope 24, 48 and 72 h after exposure of the cells to various concentrations of TQ (1, 2.5, 5, 10, 50 and 100 μg/ml) and Cis-platinum (0.5, 1, 1.5, 2, 3, 6 and 12.5 μg/ml). Moreover, apoptosis was analyzed with a flow-cytometry method. The untreated cells were considered as control group. Results: Morphological changes such as reduced cell number and increased intercellular distance and reduced cell viability in ACHN and GP-293cell lines were observed in both TQ and Cis- platinum groups; however, Cis-platinum had greater effect on ACHN cell line than GP-293 cell line. In addition, GP-293 cell line was more sensitive to TQ compared to ACHN cell line. Furthermore, TQ and Cis-platinum had apoptotic effects on both ACHN and GP-293 cell lines. Conclusion: Our findings demonstrated that TQ and Cis-platinum had cytotoxic and apoptotic effects on both cell lines, However, GP-293 cell line was more sensitive to TQ. Additionally, Cis-platinum was more effective on ACHN cell line than on GP-293 cell line.

SGT 세포주에서 유로키나제형 플라스미노젠 활성제 수용체의 높은 발현

최현식,박경주,천윤권,이종헌 대한구강악안면병리학회 2011 대한구강악안면병리학회지 Vol.35 No.6

Urokinase-type plasminogen activator(uPA) bound to urokinase plasminogen activator receptor(uPAR) expression is strongly correlated with the metastatic potential of various tumors by enhancing ECM degradation through plasminogen and matrix metallopreotease activation. But expression of uPA/uPAR in human malignant salivary gland tumors has been rarely reported. The purpose of this study were to investigate mRNA expression and cytologic concentration of uPAR in SGT cell line compared to various cancer cell lines by RT-PCR and ELISA method, and to study migration and adhesion assay. These results would be to apply the pathogenesis and prognosis of malignant salivary gland tumors. All the cell lines(SGT, HN 4, SCC 25, and HeLA) were cultured under DMEM with 10% FBS at 37℃ in a 5% CO2 incubator. We studied a possible association between mRNA expression and cytosolic concentrations of uPAR in SGT cell line compared to various cancer cell lines using RT-PCR and an enzyme-linked immunoassay( ELISA) method. And also cell adhesion and migration assay were done in all the cell lines. In migration assay SGT cell line was about 2.5-4 folds higher than another cell lines. In adhesion assay SGT cell line was about 1.1-2 folds higher than another cell lines. uPAR cytosolic concentrations of SGT cell line was about 3.4-10 folds by ELISA, while mRNA expression was about 2.5-5 folds by RT-PCR. Oral Scc cell lines showed the lowest value. uPAR protein and mRNA expression were correlated with migration and adhesion assay. From the aboving results, high cytosolic concentrations and mRNA expression of uPAR were correlated with migration and adhesion assay. It suggested that this might be a specific marker for malignant potential of SGT cell line and would be contributed to pathogenesis and prognosis of human salivary gland adenocarcinoma

KUMA3 모세포주와 변이주에서 EGF에 대한 반응

오해일(Hai-Ill Oh),문용석(Yong-Suk Moon),최인장(In-Jang Choi) 대한해부학회 2002 Anatomy & Cell Biology Vol.35 No.6

세포의 신호전달체계 통로와 연관된 많은 성장인자 중 epidermal growth factor (EGF)의 기전이 가장 잘 밝혀져 있다. EGF는 표피의 재생과 EGF 수용체가 과발현되는 상피암의 약제치료에 항암제와 병용으로 사용함으로써 항암 효과를 상승시킨다. 그러나 과도한 EGF사용으로 생길 수 있는 부작용을 최소화하기 위해 아랫입술 편평상피암 조직에서 확립한KUMA3 모세포주와 과량의 EGF가 첨가된 배양액에서 장기간 계대배양으로 획득한 변이주를 대상으로 EGF에 대한 반응들을 관찰하였다. EGF 처리에 대한 세포군락 형성 형태는 모세포주에서는 세포들이 분산된 상태로 뚜렷한 세포돌기들이 관찰되는 반면에 변이주에서는 대조군과 유사한 형태로 둥근 세포들로 밀집된 상태였다. 대조군에 대한 EGF처리군의 세포군락수의 비율은 EGF농도에 따라 두 세포주에서 같은 양상으로 줄었으나, 변이주에서 세포군락수의 비율은 모세포주보다 더 높았다. 모세포주 세포와 변이주 세포를 같은 수로 동시에 배양하였을 때 EGF가 첨가되지 않은 배양액에서는 모세포주 세포가 우성적으로 성장하였으나, EGF가 첨가된 배양액들에서는 변이주 세포가 우세하게 자랐다. EGF에 의한 세포분화나 고사 및 증식에 관여하는 bc1-2, p53과 PCNA의 단백 변화를 휴지기 상태의 세포에 3, 6, 9, 12, 24시간으로 EGF로 처리하여 Western blot으로 관찰하였으나 두 세포주에서 변화의 차이가 없었다. EGF에 의한 EGF 수용체 단백의 세포 내 유입 후 탈감작이 모세포주에서는 EGF처리군 모두 동일한 상태였으나, 변이주에서는 EGF처리 시간별에 따른 EGF수용체 단백이 주 기성을 나타내었다. EGF 수용체 tyrosine kinase의 기질인 annexin I과 annexin II의 발현 변화는 모세포주에서는annexin I과 annexin II의 변화가 뚜렷하지 않았고, 변이주에서는 annexin I의 변화는 뚜렷하지 않았으나 annexin II는 EGF처리 12시간과 24시간에서 현저하게 감소하였다. 이러한 annexin II의 현저한 감소를 정확히 판명하기 위해 면역세포화학법으로 관찰하였을 때 모세포주에서는 EGF처리 9시간에서 세포질에 편재해 있던 annexin II가 핵막 쪽으로 이동되었으나 변이주에서는 annexin II가 세포막 쪽으로 이동되어 있었다. 변이주의 EGF처리 12시간과 24시간에서 annexin II가 현저하게 감소된 것은 annexin II가 세포밖으로 분비되었기 때문이다. 이상의 결과들을 통해 변이주에서 EGF처리에도 불구하고 모세포주와 다르게 세포군락 형성 형태와 세포모양이 대조군과 유사하게 유지될 수 있는 것은 EGF 수용체 단백의 EGF처리에 따른 시간별 주기성과 EGF처리로 인한 annexin II의 세포 밖 분비에 기인한 것으로 생각되어진다. 그러므로 이러한 결과들은 여러 상피암에 약제치료 시 항암제에 EGF 병용여부를 결정하는 좋은 자료가 될 것으로 생각된다. EGF has been a representive growth factor for understanding the signal transduction that underlies the biological response of a number of growth factors. EGF plays an important role in the regulation of growth inhibition in the squamous cell carcinoma with over-expressed EGF receptors, but the mechanism that determine sensitivity to the growth inhibition by EGF are not well understood. The KUMA3 parental cell line, derived from a squamous cell carcinoma of the human lower lip, was established and variant cell line derived from the parental cell line was acquired by treatment with high dose EGF (200 ng/mL) for 6 months. The KUMA3 parental cells treated with EGF showed marked scattering cells with prominent dendritic processes. On the other hand, EGF treated cells in the variant cell line showed a dense monolayer of ovoid cells similar to untreated cells. Therefore, it was conformed that the variant cell line was resistant to the growth-inhibitory effect of EGF. To gain insight into the action mechanism of EGF in the KUMA3 parental and variant cell lines, Western blot analysis was examined to identify transregulation of EGF receptor. In the all parental cell lines treated with EGF, EGF receptor proteins were completely disappeared by lysosomal degradation. However, EGF receptor proteins were shown the periodic appearance according to the time course in the variant cell lines treated with EGF. In Western blot and immunocytochemical analyses, annexin II protein evenly distributed in the cytoplasm relocated to the plasma membrane at 9 hrexposure to EGF and was significantly decreased at 12 hr- and 24 hr-exposures to EGF in the variant cell line. However, annexin II protein relocated to the nuclear membrane at 9 hr-exposure to EGF and was not any change in amount at 12 hr- and 24-exposure to EGF in the parental cell line. In conclusion, this study suggests that the retention of colony formation pattern and cell morphology in the variant cell line treated with EGF might be owing to the heterologous desensitization of EGF receptor and exocytosis of annexin II.

Establishment and characterization of cell lines from three human thyroid carcinomas: Responses to all-trans-retinoic acid and mutations in the BRAF gene

Koh, C.S.,Ku, J.L.,Park, S.Y.,Kim, K.H.,Choi, J.S.,Kim, I.J.,Park, J.H.,Oh, S.K.,Chung, J.K.,Lee, J.H.,Kim, W.H.,Kim, C.W.,Cho, B.Y.,Park, J.G. North-Holland 2007 Molecular and cellular endocrinology Vol.264 No.1

We report the characteristics of three cell lines (designated, SNU-80, SNU-373 and SNU-790), which were established from two papillary carcinomas and one anaplastic carcinoma obtained from three Korean thyroid carcinoma patients. All cell lines grow as adherent cells. Electron microscopy characteristically showed cytoplasmic invaginations of nuclei and intranuclear cytoplasmic inclusions. SNU-80 and SNU-790 cells showed a positive reaction to anti-cytokeratin antibody, and SNU-790 cells positivity for CK-19. All lines were free of mycoplasma or bacteria and were proven unique by DNA fingerprinting analysis. The p15 and p16 genes are deleted in the SNU-790 line. Mutations of the p53 gene were found in two lines (SNU-80 and SNU-373), but no mutations in the RET or MEN1 genes were observed. Mutations of the BRAF gene were found in the SNU-80 (G468R) and the SNU-790 (V599E) cell lines, but no mutations in the K-ras gene were present. SNU-80 and SNU-790 cells showed a positive reaction to anti-cytokeratin antibody, and no evidence of the production of thyroglobulin or calcitonin was observed. The cell lines were unable to trap radioactive iodine but did not contain TSH receptor. In addition, we investigated the mRNA expression levels of Tg, TSHR, TTF-1, PAX-8, NIS, IL-6, and LIF, and of the α, β and γ retinoic acid receptors in these cell lines. IL-6 was down-regulated in all three cell lines by all-trans-retinoic acid treatment. RAR-α was expressed but RAR-β was not expressed in the three cell lines, and RAR-γ was not expressed in SNU-790. Interestingly, RAR-β (SNU-80 and SNU-373) and RAR-γ (SNU-790) was up-regulated by all-trans-retinoic acid treatment. We believe that these well-characterized thyroid carcinoma cell lines may be useful tools for investigations on the biological characteristics of thyroid carcinoma, particularly for investigations related to gene alterations, especially of the BRAF gene. These cell lines may also be useful for redifferentiation therapy studies on thyroid carcinoma using all-trans-retinoic acid.

Higher Expression of Urokinase - type Plasminogen Activator And Plasminogen Activator Inhibitor Type 1 in SGT Cell Line

이종헌,유상원,박경주 대한구강악안면병리학회 2010 대한구강악안면병리학회지 Vol.34 No.3

Urokinase-type plasminogen activator (uPA) and plasminogen activator type 1 (PAI-1) inhibitor contribute to the invasiveness of many carcinomas. It will be helpful to study clinical behavior of patients with malignant tumor by analysis of their expression. Expression of uPA and PAI-1 in human salivary gland tumors has been rarely reported in vitro. The purpose of this study were to investigate the protein expression of uPA and PAI-1 in SGT cell line compared to oral SCC and HeLa cell lines and to study migration and adhesion assay. All the cell lines were cultured under DMEM with 10% FBS at at 37oC in a 5% CO2 incubator. We studied a possible association between cytosolic uPA and PA-1 concentrations in SGT cell line compared to any other cell lines through cell migration and adhesion assay, and enzyme-linked immunoassay(ELISA). In migration assay SGT cell line was about 2 .5-4 folds higher than another cell lines. In adhesion assay SGT cell line was about 1.1-2 folds higher than another cell lines. uPA cytosolic concentrations of SGT cell line was about 3-10 folds, while PAI-1 was about 2.5-10 folds. Oral SCC cell lines were the lowest value. Both uPA and PAI-1 concentrations were correlated with migration and adhesion assay. High cytosolic concentrations of uPA and PAI-1 was correlated with migration and adhesion assay. It suggested that these markers might be specific marker for SGT cell line and would be contributed to treatment and prognosis of human salivary gland adenocarcinoma.

SGT 세포주에서 유로키나제형 플라스미노젠 활성제 및 제일형 플라스미노젠 활성억제제의 높은 발현

유상원,박경주,이종헌 대한구강악안면병리학회 2010 대한구강악안면병리학회지 Vol.34 No.3

Urokinase-type plasminogen activator (uPA) and plasminogen activator type 1 (PAI-1) inhibitor contribute to the invasiveness of many carcinomas. It will be helpful to study clinical behavior of patients with malignant tumor by analysis of their expression. Expression of uPA and PAI-1 in human salivary gland tumors has been rarely reported in vitro. The purpose of this study were to investigate the protein expression of uPA and PAI-1 in SGT cell line compared to oral SCC and HeLa cell lines and to study migration and adhesion assay. All the cell lines were cultured under DMEM with 10% FBS at at 37oC in a 5% CO2 incubator. We studied a possible association between cytosolic uPA and PA-1 concentrations in SGT cell line compared to any other cell lines through cell migration and adhesion assay, and enzyme-linked immunoassay(ELISA). In migration assay SGT cell line was about 2 .5-4 folds higher than another cell lines. In adhesion assay SGT cell line was about 1.1-2 folds higher than another cell lines. uPA cytosolic concentrations of SGT cell line was about 3-10 folds, while PAI-1 was about 2.5-10 folds. Oral SCC cell lines were the lowest value. Both uPA and PAI-1 concentrations were correlated with migration and adhesion assay. High cytosolic concentrations of uPA and PAI-1 was correlated with migration and adhesion assay. It suggested that these markers might be specific marker for SGT cell line and would be contributed to treatment and prognosis of human salivary gland adenocarcinoma