Nonlinear mixed models for characterization of growth trajectory of New Zealand rabbits raised in tropical climate

de Sousa, Vanusa Castro,Biagiotti, Daniel,Sarmento, Jose Lindenberg Rocha,Sena, Luciano Silva,Barroso, Priscila Alves,Barjud, Sued Felipe Lacerda,de Sousa Almeida, Marisa Karen,da Silva Santos, Natana Asian Australasian Association of Animal Productio 2022 Animal Bioscience Vol.35 No.5

Objective: The identification of nonlinear mixed models that describe the growth trajectory of New Zealand rabbits was performed based on weight records and carcass measures obtained using ultrasonography. Methods: Phenotypic records of body weight (BW) and loin eye area (LEA) were collected from 66 animals raised in a didactic-productive module of cuniculture located in the southern Piaui state, Brazil. The following nonlinear models were tested considering fixed parameters: Brody, Gompertz, Logistic, Richards, Meloun 1, modified Michaelis-Menten, Santana, and von Bertalanffy. The coefficient of determination (R²), mean squared error, percentage of convergence of each model (%C), mean absolute deviation of residuals, Akaike information criterion (AIC), and Bayesian information criterion (BIC) were used to determine the best model. The model that best described the growth trajectory for each trait was also used under the context of mixed models, considering two parameters that admit biological interpretation (A and k) with random effects. Results: The von Bertalanffy model was the best fitting model for BW according to the highest value of R² (0.98) and lowest values of AIC (6,675.30) and BIC (6,691.90). For LEA, the Logistic model was the most appropriate due to the results of R² (0.52), AIC (783.90), and BIC (798.40) obtained using this model. The absolute growth rates estimated using the von Bertalanffy and Logistic models for BW and LEA were 21.51g/d and 3.16 cm², respectively. The relative growth rates at the inflection point were 0.028 for BW (von Bertalanffy) and 0.014 for LEA (Logistic). Conclusion: The von Bertalanffy and Logistic models with random effect at the asymptotic weight are recommended for analysis of ponderal and carcass growth trajectories in New Zealand rabbits. The inclusion of random effects in the asymptotic weight and maturity rate improves the quality of fit in comparison to fixed models.

Genetic evaluation of sheep for resistance to gastrointestinal nematodes and body size including genomic information

Torres Tatiana Saraiva,Sena Luciano Silva,Santos Gleyson Vieira dos,Figueiredo Filho Luiz Antonio Silva,Barbosa Bruna Lima,Júnior Antônio de Sousa,Britto Fábio Barros,Sarmento José Lindenberg Rocha 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.4

Objective: The genetic evaluation of Santa Inês sheep was performed for resistance to gastrointestinal nematode infection (RGNI) and body size using different relationship matrices to assess the efficiency of including genomic information in the analyses. Methods: There were 1,637 animals in the pedigree and 500, 980, and 980 records of RGNI, thoracic depth (TD), and rump height (RH), respectively. The genomic data consisted of 42,748 SNPs and 388 samples genotyped with the OvineSNP50 BeadChip. The (co)variance components were estimated in single- and multi-trait analyses using the numerator relationship matrix (A) and the hybrid matrix H, which blends A with the genomic relationship matrix (G). The BLUP and single-step genomic BLUP methods were used. The accuracies of estimated breeding values and Spearman rank correlation were also used to assess the feasibility of incorporating genomic information in the analyses. Results: The heritability estimates ranged from 0.11±0.07, for TD (in single-trait analysis using the A matrix), to 0.38±0.08, for RH (using the H matrix in multi-trait analysis). The estimates of genetic correlation ranged from –0.65±0.31 to 0.59±0.19, using A, and from –0.42±0.30 to 0.57±0.16 using H. The gains in accuracy of estimated breeding values ranged from 2.22% to 75.00% with the inclusion of genomic information in the analyses. Conclusion: The inclusion of genomic information will benefit the direct selection for the traits in this study, especially RGNI and TD. More information is necessary to improve the understanding on the genetic relationship between resistance to nematode infection and body size in Santa Inês sheep. The genetic evaluation for the evaluated traits was more efficient when genomic information was included in the analyses. Objective: The genetic evaluation of Santa Inês sheep was performed for resistance to gastrointestinal nematode infection (RGNI) and body size using different relationship matrices to assess the efficiency of including genomic information in the analyses.Methods: There were 1,637 animals in the pedigree and 500, 980, and 980 records of RGNI, thoracic depth (TD), and rump height (RH), respectively. The genomic data consisted of 42,748 SNPs and 388 samples genotyped with the OvineSNP50 BeadChip. The (co)variance components were estimated in single- and multi-trait analyses using the numerator relationship matrix (A) and the hybrid matrix H, which blends A with the genomic relationship matrix (G). The BLUP and single-step genomic BLUP methods were used. The accuracies of estimated breeding values and Spearman rank correlation were also used to assess the feasibility of incorporating genomic information in the analyses.Results: The heritability estimates ranged from 0.11±0.07, for TD (in single-trait analysis using the A matrix), to 0.38±0.08, for RH (using the H matrix in multi-trait analysis). The estimates of genetic correlation ranged from –0.65±0.31 to 0.59±0.19, using A, and from –0.42±0.30 to 0.57±0.16 using H. The gains in accuracy of estimated breeding values ranged from 2.22% to 75.00% with the inclusion of genomic information in the analyses.Conclusion: The inclusion of genomic information will benefit the direct selection for the traits in this study, especially RGNI and TD. More information is necessary to improve the understanding on the genetic relationship between resistance to nematode infection and body size in Santa Inês sheep. The genetic evaluation for the evaluated traits was more efficient when genomic information was included in the analyses.

Effects of zinc oxide and calcium–doped zinc oxide nanocrystals on cytotoxicity and reactive oxygen species production in different cell culture models

Souza Gabriela Leite de,Moura Camilla Christian Gomes,Silva Anielle Christine Almeida,Marinho Juliane Zacour,Silva Thaynara Rodrigues,Dantas Noelio Oliveira,Bonvicini Jéssica Fernanda Sena,Turrioni An 대한치과보존학회 2020 Restorative Dentistry & Endodontics Vol.45 No.4

Objectives: This study aimed to synthesize nanocrystals (NCs) of zinc oxide (ZnO) and calcium ion (Ca2+)-doped ZnO with different percentages of calcium oxide (CaO), to evaluate cytotoxicity and to assess the effects of the most promising NCs on cytotoxicity depending on lipopolysaccharide (LPS) stimulation. Materials and Methods: Nanomaterials were synthesized (ZnO and ZnO:xCa, x = 0.7; 1.0; 5.0; 9.0) and characterized using X-ray diffractometry, scanning electron microscopy, and methylene blue degradation. SAOS-2 and RAW 264.7 were treated with NCs, and evaluated for viability using the MTT assay. NCs with lower cytotoxicity were maintained in contact with LPS-stimulated (+LPS) and nonstimulated (−LPS) human dental pulp cells (hDPCs). Cell viability, nitric oxide (NO), and reactive oxygen species (ROS) production were evaluated. Cells kept in culture medium or LPS served as negative and positive controls, respectively. One-way analysis of variance and the Dunnett test (α = 0.05) were used for statistical testing. Results: ZnO:0.7Ca and ZnO:1.0Ca at 10 µg/mL were not cytotoxic to SAOS-2 and RAW 264.7. +LPS and −LPS hDPCs treated with ZnO, ZnO:0.7Ca, and ZnO:1.0Ca presented similar NO production to negative control (p > 0.05) and lower production compared to positive control (p < 0.05). All NCs showed reduced ROS production compared with the positive control group both in +LPS and −LPS cells (p < 0.05). Conclusions: NCs were successfully synthesized. ZnO, ZnO:0.7Ca and ZnO:1.0Ca presented the highest percentages of cell viability, decreased ROS and NO production in +LPS cells, and maintenance of NO production at basal levels.

Antiplasmodial and Cytotoxic Activities of Toad Venoms from Southern Amazon, Brazil

Felipe Finger Banfi,Karla de Sena Guedes,Carla Regina Andrighetti,Ana Carolina Aguiar,Bryan Wender Debiasi,Janaina da Costa Noronha,Domingos de Jesus Rodrigues,Gerardo Magela Vieira Junior,Bruno Anton 대한기생충학열대의학회 2016 The Korean Journal of Parasitology Vol.54 No.4

Final report on APMP.QM-S5: Essential and toxic elements in seafood

Valiente, Liliana,Bennett, John W,de Sena, Rodrigo Caciano,Kotzeva, Boriana,Massiff, Gabriela,Chao, Jingbo,Wang, Jun,Nasr, Randa,Labarraque, Guillaume,Kakoulidis, Elias,Lampi, Eugenia,Sin, Della Wai-m Springer-Verlag 2013 Metrologia Vol.50 No.-

<P>The supplementary comparison APMP.QM-S5 was undertaken to demonstrate the capability of participating national metrology institutes (NMIs) and designated institutes (DIs) in measuring the contents of the incurred essential elements (iron and zinc) and toxic elements (total arsenic and cadmium) at µg/g levels in a test sample of dried shrimp by various analytical techniques.</P><P>At the APMP TCQM Meeting held in Pattaya, Thailand in November 2010, Government Laboratory of the Government of the Hong Kong Special Administrative Region (GLHK) proposed this APMP supplementary comparison. The proposal was further discussed and agreed upon at the CCQM Inorganic Analysis Working Group Meeting held in Paris in April 2011. GLHK was the coordinating laboratory for the supplementary comparison. For enhancing the collaboration amongst specialized regional bodies in Asia-Pacific and to help build the laboratory capacity of NMIs/DIs from developing economies, the reference values of the supplementary comparison are used for evaluation of performance of participants of an APMP proficiency testing programme (APMP PT 11-01), an Asia-Pacific Laboratory Accreditation Cooperation proficiency testing programme (APLAC T082) and an Asia-Pacific Economic Co-operation proficiency testing programme (APEC PT), which were concurrently run using the same testing material as in APMP.QM-S5.</P><P>The supplementary comparison serves to facilitate claims by participants on the Calibration and Measurement Capabilities (CMCs) as listed in Appendix C of the Key Comparison Database (KCDB) under the Mutual Recognition Arrangement of the International Committee for Weights and Measures (CIPM MRA).</P><P>A total of 18 institutes registered for the supplementary comparison and all of them submitted their results. Most of the participants used microwave acid digestion methods for sample dissolution. For the instrumental determination, a variety of techniques like ICP-MS, ICP-OES, INAA and AAS were employed by the participants. For this supplementary comparison, inorganic core capabilities have been demonstrated by concerned participants with respect to methods including ICP-MS (without isotope dilution), ID-ICP-MS, ICP-OES, INAA and AAS on the determination of total arsenic, cadmium, iron and zinc in a matrix of seafood.</P><P>Main text.To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/.</P><P>The final report has been peer-reviewed and approved for publication by the APMP, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).</P>

Epithelial Barrier Theory: The Role of Exposome, Microbiome, and Barrier Function in Allergic Diseases

Losol Purevsuren,Sokolowska Milena,Hwang Yu-Kyoung,Ogulur Ismail,Mitamura Yasutaka,Yazici Duygu,Pat Yagiz,Radzikowska Urszula,Ardicli Sena,Yoon Jeong-Eun,Choi Jun-Pyo,Kim Sae-Hoon,van de Veen Willem,A 대한천식알레르기학회 2023 Allergy, Asthma & Immunology Research Vol.15 No.6

Allergic diseases are a major public health problem with increasing prevalence. These immune-mediated diseases are characterized by defective epithelial barriers, which are explained by the epithelial barrier theory and continuously emerging evidence. Environmental exposures (exposome) including global warming, changes and loss of biodiversity, pollution, pathogens, allergens and mites, laundry and dishwasher detergents, surfactants, shampoos, body cleaners and household cleaners, microplastics, nanoparticles, toothpaste, enzymes and emulsifiers in processed foods, and dietary habits are responsible for the mucosal and skin barrier disruption. Exposure to barrier-damaging agents causes epithelial cell injury and barrier damage, colonization of opportunistic pathogens, loss of commensal bacteria, decreased microbiota diversity, bacterial translocation, allergic sensitization, and inflammation in the periepithelial area. Here, we review scientific evidence on the environmental components that impact epithelial barriers and microbiome composition and their influence on asthma and allergic diseases. We also discuss the historical overview of allergic diseases and the evolution of the hygiene hypothesis with theoretical evidence.

Nutrition and yield of hydroponic arugula under inoculation of beneficial microorganisms

Gato Isabela Martins Bueno,da Silva Oliveira Carlos Eduardo,Oliveira Thaissa Julyanne Soares Sena,Jalal Arshad,de Almeida Moreira Vitória,Giolo Victoria Moraes,Vitória Letícia Schenaide,de Lima Bruno 한국원예학회 2023 Horticulture, Environment, and Biotechnology Vol.64 No.2

The use of benefi cial fungi and bacteria can improve growth, yield and food quality of plants. The electrical conductivity (EC) of a nutrient solution is closely linked to better nutrition of arugula plants in a hydroponic system. Therefore, objectives of current study were to evaluate isolated and combined eff ect of inoculation with Azospirillum brasilense and Trichoderma hazianum under two ECs on root-shoot biomass, nutrition and yield of arugula in hydroponic cultivation system. The experi- ment was designed in a strip-plot block with fi ve replications in a 4 × 2 factorial scheme. The treatments were consisted of four microbial inoculations (without, A. brasilense, T. harzianum and co-inoculation of both) and two ECs (1.4 and 1.6 dS m −1). The higher EC with A. brasilense inoculation provided greater growth of arugula plants in a hydroponic system. Inoculation with A. brasilense has increased leaf area, shoot–root length, leaf chlorophyll index (LCI) and shoot ammonium accumulation while reduced root-shoot nitrate accumulation. The leaf nutrition or biofortifi cation of arugula plants with K, S, Ca, Mg, Zn, Fe, and Mn was improved under both isolated and combined inoculations of A. brasilense and T. harzianum .