[PB-0095] Genome-Wide Association Study and Transcriptome Analysis of Salinity Tolerance in Peanuts(Arachis hypogaea L.)

Kunyan Zou(Kunyan Zou),Seung-Ah Han(Seung-Ah Han),Min-jae Choi(Min-jae Choi),Min-jeong Lee(Min-jeong Lee),Da-Hye Jeon(Da-Hye Jeon),Tae-Hwan Jun(Tae-Hwan Jun) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

[PB-0094] Genome-Wide Association Study of Leaf Chlorophyll Content Using High-Density 50K SNP Array in Peanut Accessions

Kunyan Zou(Kunyan Zou),Nayoung Kwon(Nayoung Kwon),Tae-Hwan Jun(Tae-Hwan Jun) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

Dynamic response characteristics of an innovative turretless low motion FPSO hull in central GoM ultra-deep waters

Zou, Jun Techno-Press 2022 Ocean systems engineering Vol.12 No.2

In oil and gas industry, FPSO concept is the most popular hull form and ship shaped hull form dominants the FPSO market. Only a non-ship-shaped hull in operations with minor market shares is the cylindrical FPSO hull with medium to small storage capability. To add contracting options and competitions to reduce field development costs, an innovative turretless low motion hull, eco-FPSO, with 1MM bbls oil storage capacity and suitable for installing topsides modulars and equipping with regular SCRs, was first introduced in Zou (2020a). Dynamic characteristic responses of the eco-FPSO compared to the traditional SS-FPSO hull and DD-Semi platform are presented and discussed in this paper, suitability and feasibility of the proposed hull have been demonstrated and validated through extensive analyses in 10-yrp, 100-yrp and 1,000-yrp hurricanes in ultra-deepwater central GoM.

자기 공명 영상을 이용한 안면비대칭환자의 측두하악관절 원반의 형태와 위치에 관한 연구

Bingshuang Zou,김태우,최순철 대한치과교정학회 2005 대한치과교정학회지 Vol.35 No.5

본 연구는 안면비대칭이 있는 환자의 측두하악관절원반 형태와 위치를 평가하고 악관절내장증과 안명비대칭 간의 관계를 규명하기 위하여 시행되었다. 남자 10명 및 여자 27명으로 구성된 안명비대칭 환자 37명의 74개의 TMJ MRI를 사용하였으며, 대상 집단의 연령은 12세에서 44세까지의 범위로 평균 21.4세였다. 시상 및 두정 TMJ MRI를 최대감합위 및 최대개구위에서 채득한 후 부분전방변위, 전방변위, 회전 또는 측방 원반변위 등 원반변위의 종류를 기록하였으며, 원반의 모양, 위치, 그리고 원반의 변위 및 회전을 MRI tracing 상에서 판독하였다. 악관절내장증의 증상은 정상, 정복성 전방원반변위 (ADDR). 그리고 비정복성 전방원반변위(ADDNR)집단으로 분류하였으며, 환자는 양측성 정상. 편측 혹은 양측 내장증 집단으로 분류하였다. 약 70%의 환자에서 편측성 또는 양측성 내장증을 보였으며, 통계분석 결과 ADD. 특히 회전성 ADD가 변위측에서 더 높은 빈도로 나타났으나 반대측에서는 원반의 위치가 정상인 경우가 많았다 (p<0.01).변위측의 원반은 모양의 변형 및 하전방 변위를 유의하게 더 나타냈다. 그러나 반대쪽은 개구시 원반이 수직방향으로 과운동성이 관찰되었다. 이 연구는 안면비대칭 환자에서 ADD의 종류가 원반의 모양,경사,변위 각도,변위의 수직거리 및 회전각도와 연관이 있다는 것을 제시한다. The present study was conducted to examine the morphometrics and function of the disk on both sides among patients with facial asymmetry (FA) and to elucidate plausible correlations between internal derangement (ID) and FA. The sample was composed of 10 males and 27 females with FA. The disk status of all subjects was evaluated by biateral high resolution magnetic resonance scans in the sagittal (closed and open) and coronal (closed) planes. Five types of disk displacement were identified accordingly. The disk function was diagnosed as normal disk function, disk displacement with reduction, and disk displacement without reduction. The disk shape on sagittal MRI in closed position was classified as bi-concave, biplanar, funnel/hemiconvex, and deformed. The disk position, translation and rotation were also measured. The difference between the shifted side and non-shifted side was analyzed by statistical analysis. Approximately 70% of the patients in the present study showed unilateral or bilateral ID. It was found that anterior disk displacements (ADD), especially rotational ADD, occurred more frequently in the shifted side, while normal disk position was observed mainly in the non-shifted side(p<0.01). The disk of the shifted side showed significantly deformed configuration and inferior-anterior disk position. However, the disk of the non-deviated side showed hyper-mobility during jaw opening movement. These results demonstrate that in FA patients, the disks status of the shifted side is different from that of the non-shifted side, a phenomenon that could be correlated to facial asymmetry.

Substituting oat hay or maize silage for portion of alfalfa hay affects growth performance, ruminal fermentation, and nutrient digestibility of weaned calves

Yang Zou,XinPing Zou,XiZhi Li,Gang Guo,Peng Ji,Yan Wang,ShengLi Li,YaJing Wang,ZhiJun Cao 아세아·태평양축산학회 2018 Animal Bioscience Vol.31 No.3

Objective: The impact of forage feeding strategy on growth performance, ruminal fermentation and nutrient digestibility in post-weaning calves was investigated. Methods: Forty-five female Holstein calves (body weight [BW] = 79.79±0.38 kg) were enrolled in the 35-d study at one week after weaning and randomly assigned to one of three dietary treatments. All diets were fed as total mixed ration containing 60% (dry matter [DM] basis) of basal starter feed and 40% (DM basis) of forage, but varied in composition of forage source including i) alfalfa (40% DM, AH); ii) alfalfa hay (26.7% DM)+oat hay (13.3% DM; OH); iii) alfalfa hay (26.7% DM)+corn silage (13.3% DM; WS). Results: Dry matter intake was not different among treatment groups (p>0.05). However, BW (p<0.05) and average daily gain (p<0.05) of calves fed AH and OH were greater than WS-fed calves, whereas heart girth was greater in OH-fed calves than those fed AH and WS (p<0.05). Ruminal fermentation parameters including proportion of butyric acid, acetated-to-propionate ratio, concentration of total volatile fatty acid, protozoal protein, bacterial protein, and microbial protein in rumen were the highest in OH (p<0.05) and the lowest in WS. Compared with the AH and WS, feeding oat hay to postweaning calves increased crude protein digestibility (p<0.05), and decreased duration of diarrhea (p<0.05) and fecal index (p<0.05). Conclusion: Our results suggested that partially replacing alfalfa hay with oat hay improved ruminal fermentation, nitrogen utilization, and reduced incidence of diarrhea in post-weaning dairy calves.

Responses to Starch Infusion on Milk Synthesis in Low Yield Lactating Dairy Cows

Zou, Yang,Yang, Zhanshan,Guo, Yongqing,Li, Shengli,Cao, Zhijun Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.9

The effect of starch infusion on production, metabolic parameters and relative mRNA abundance was investigated in low yield lactating cows from 86 days in milk. Six Holstein cows fitted with permanent ruminal cannulas were arranged into one of two complete $3{\times}3$ Latin squares and infused with a starch solution containing 800 grams starch for 16 days. The three treatments were: i) ruminal and abomasal infusion with water (Control); ii) ruminal infusion with cornstarch solution and abomasal infusion with water (Rumen); iii) ruminal infusion with water and abomasal infusion with cornstarch solution (Abomasum). There were no significant differences (p>0.05) among the three treatments with low yield lactating cows in feed and energy intake, milk yield and composition, plasma metabolism, or even on gene expression. However, cows receiving starch through rumen performed better than directly through the abomasum during the glucose tolerance test procedure with a higher area under the curve (AUC; p = 0.08) and shorter half-time ($t^{1/2}$; p = 0.11) of plasma insulin, therefore, it increased glucose disposal, which stated a lipid anabolism other than mobilization after energy supplementation. In conclusion, extra starch infusion at concentration of 800 g/d did not enhance energy supplies to the mammary gland and improve the lactating performance in low yield lactating cows.

Integrated Metabolome and Transcriptome Analysis Reveal Complex Molecular Mechanisms Underlying Selenium Response of Aloe vera L.

Zou Yi,Changyou Han,Fang Wang,Yanhua Tan,Saina Yang,Chuying Huang,Shengyou Xie,Xueqin Xiao 한국식물학회 2021 Journal of Plant Biology Vol.64 No.2

Aloe vera L. is an excellent resource for medication. Selenium-enriched aloe can act as a functional food to human health. To understand the molecular mechanisms underlying selenium accumulation in Aloe vera L., we characterized the metabolic and transcriptome responses of aloe leaves under different Na2SeO4 levels (0, 200, and 400 mg/L) treatments. Aloe leaves spraying with exogenous selenium fertilizer showed a significant increase in total Se content compared with those under non-treatment control, and no distinct differences were observed between 200 and 400 mg/L Se treatment. Non-targeted metabolic profiling revealed that Se treatment triggered the accumulation of antioxidants, including amino acid and derivatives, phenols, flavonoids, terpene, as well as indole derivatives. Consistent with metabolic changes following Se treatment, the transcript level of genes involved in Se assimilation and Se-response showed dramatically increase, such as those encoding sulfate transporter, antioxidants, phytohormone signaling, transcription factors, and phenols metabolites, suggesting Se assimilation generally accompanied with antioxidant and pathogen defense. This study exhibited comprehensive insights on Se response in Aloe vera L., and provided us with targeted genes for genetic engineering, thereby improving the therapeutic value of aloe.

SPECTRAL RADIUS OF BIORTHOGONAL WAVELETS WITH ITS APPLICATION

Zou, Qingyun,Wang, Guoqiu,Yang, Mengyun Korean Mathematical Society 2014 대한수학회지 Vol.51 No.5

In this paper, a 2-circular matrix theory is developed, and a concept of spectral radius for biorthogonal wavelet is introduced. We propose a novel design method by minimizing the spectral radius and obtain a wavelet which has better performance than the famous 9-7 wavelet in terms of image compression coding.

Genetic Diversity and Population Structure of Peanut (Arachis hypogaea L.) Accessions from Five Different Origins

Zou, Kunyan,Kim, Ki-Seung,Lee, Daewoong,Jun, Tae-Hwan The Korean Society of Crop Science 2020 한국작물학회지 Vol.65 No.4

Peanut is an allotetraploid derived from a single recent polyploidization. Polyploidization has been reported to have caused significant loss in genetic diversity during the domestication of cultivated peanuts. Single nucleotide polymorphism (SNP)-based markers such as cleaved amplified polymorphic sequences (CAPS) derived from next-generation sequencing (NGS) have been developed and widely applied for breeding and genetic research in peanuts. This study aimed to identify the genetic diversity and population structure using 30 CAPS markers and 96 peanut accessions from five different origins. High genetic dissimilarities were detected between the accessions from Korea and those from the other three South American origins generally regarded as the origin of peanuts, while the accessions from Brazil and Argentina presented the lowest genetic dissimilarity. Based on the results of the present study, accessions from Korea have unique genetic variation compared to those from other countries, while accessions from the other four origins are closely related. Our study identified the genetic differentiation in 96 peanut accessions from five different origins, and this study also showed the successful application of SNP information derived from re-sequencing based on NGS technology.

Molecular Cloning, Segmental Distribution and Ontogenetic Regulation of Cationic Amino Acid Transporter 2 in Pigs

Zou, Shi-geng,Zhi, Ai-min,Zhou, Xiang-yan,Zuo, Jian-jun,Zhang, Yan,Huang, Zhi-yi,Xu, Ping-Wen,Feng, Ding-yuan Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.5

The goal of this study was to elucidate the expression and segmental distribution of the glomerular cationic amino acid metabolism transporter-2 (CAT-2) and thus to improve our understanding of porcine cationic amino acid transporters and amino acid absorption. Porcine CAT-2 was cloned, sequenced and characterized. The predicted amino acid sequence of porcine CAT-2 shared 86.1% and 92.1% identity with human and mouse CAT-2A, respectively. The tissue distribution patterns and ontogenic changes of CAT-2 mRNAs were determined by real-time Q-PCR. The results showed that porcine CAT-2 was highly expressed in the heart and intestinal tract (duodenum, ileum and jejunum). In addition, the mRNA of CAT-2 was found in liver, lung, kidney, brain and muscle. Within the intestinal tract, CAT-2 mRNA was most abundant in the ileum and rarely expressed in the duodenum. In the duodenum, the levels of CAT-2 mRNA reached their peak on day 7 (p<0.05) while in the jejunum, levels were low on day 1 and 7 and increased rapidly after day 26 before peaking on days 30 and 60 (p<0.05). The levels then dramatically decreased by day 90 (p<0.05). In the ileum, levels achieved their maximum on day 30 and then decreased significantly on day 60 (p<0.05).