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조홍식,Justin Zook,Todd Banner,박상혁,민병현,Karen A. Hasty,Eugene Pinkhassik,Erno Lindner 한국조직공학과 재생의학회 2014 조직공학과 재생의학 Vol.11 No.1
Self-assembled gold monolayers offer several advantages for the realization of novel modified electrodes for biosensor applications. This is due to their ability to decrease non-specific adsorption and provide for covalent attachment of biomolecules. Surfaces for these applications require the precise control of ligand density, the ability to immobilize ligands, and in situ-modulation of ligand activity. In this study, we focused our studies on the immobilization of antibody on a gold monolayer surface. We self-assembled thioctic acid onto the gold surface as an anchor point for the immobilization of anti-fibrinogen onto the surface. The modifications to the gold surface were characterized by ELISA, ellipsometry, and AFM.
Lisa V. Kalman,Vivekananda Datta,Mickey Williams,Justin M. Zook,Marc L. Salit,한진영 대한진단검사의학회 2016 Annals of Laboratory Medicine Vol.36 No.6
Characterized reference materials (RMs) are needed for clinical laboratory test development and validation, quality control procedures, and proficiency testing to assure their quality. In this article, we review the development and characterization of RMs for clinical molecular genetic tests. We describe various types of RMs and how to access and utilize them, especially focusing on the Genetic Testing Reference Materials Coordination Program (Get-RM) and the Genome in a Bottle (GIAB) Consortium. This review also reinforces the need for collaborative efforts in the clinical genetic testing community to develop additional RMs.