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- 저자 : Ziling Fan (검색결과 2 건)
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Shi Shu,Yunlong Bai,Gang Wang,Xinhuan Xiao,Ziling Fan,Jiang Zhang,Chang Zhao,Yang Zhao,Cheng Xia,Hongyou Zhang 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.6
Objective: Hypocalcemia is an important metabolic disease of dairy cows during the transition period, although the effect of hypocalcemia on biological function in dairy cows remains unknown. Methods: In this study, proteomic, mass spectrum, bioinformatics and western blotting were employed to identify differentially expressed proteins related to serum Ca concentration. Serum samples from dairy cows were collected at three time points: 3rd days before calving (day –3), the day of calving (day 0), and 3rd days after calving (day +3). According to the Ca concentration on day 0, a total of 27 dairy cows were assigned to one of three groups (clinical, subclinical, and healthy). Samples collected on day –3 were used for discovery of differentially expressed proteins, which were separated and identified via proteomic analysis and mass spectrometry. Bioinformatics analysis was performed to determine the function of the identified proteins (gene ontology and pathway analysis). The differentially expressed proteins were verified by western blot analysis. Results: There were 57 differential spots separated and eight different proteins were identified. Vitamin D-binding protein precursor (group-specific component, GC), alpha-2-macroglobulin (A2M) protein, and apolipoprotein A-IV were related to hypocalcemia by bioinformatics analysis. Due to its specific expression (up-regulated in clinical hypocalcemia and down-regulated in subclinical hypocalcemia), A2M was selected for validation. The results were consistent with those of proteomic analysis. Conclusion: A2M was as an early detection index for distinguishing clinical and subclinical hypocalcemia. The possible pathogenesis of clinical hypocalcemia caused by GC and apolipoprotein A-IV was speculated. The down-regulated expression of GC was a probable cause of the decrease in calcium concentration.
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Jia Chen,Jianhua Shao,Chunchao Zhao,Jie Shen,Ziling Dong,Wenyan Liu,Min Zhao,Judi Fan 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.6
Three new neolignans, fordianoles A-C (1–3),characterized as (7S,8R)-4-hydroxy-3,30,50-trimethoxy-80,90-dinor-8,40-oxyneolignan-7,70,9-triol, (7R,8R)-4-hydroxy-3,30,50-trimethoxy-80,90-dinor-8,40-oxyneolignan-7,70,9-triol, and (7R,8R)-4-hydroxy-3,30,50-trimethoxy-8,40-oxyneolignan-7,9,90-triol-70-one, together with an unusual c-lactone,3-(3,4-dihydroxyphenyl)-4-pentanolide (4), andtwenty-five known compounds (5–29) were isolated fromthe aerial parts of Viburnum fordiae Hance. Their structuresincluding absolute configurations were determined byspectroscopic and chemical methods. Among them, compounds6, 7, 11–15, 17–28 were isolated from the Viburnumgenus for the first time. The anti-inflammatory and antioxidantactivities of all compounds were evaluated in vitro. Compounds 15, 19, 20 and 29 showed significant inhibitoryactivity on NO production in RAW264.7 cells stimulated byLPS with IC50 values ranging from 8.60 to 13.92 lM. Meanwhile, compounds 1–4, 15, 19, 20, 22, 23, 25, 26 and29 exhibited varying antioxidant activities through DPPH,ABTS free radical scavenging and FRAP assays.
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