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- 저자 : Zichen Chen (검색결과 6 건)
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Zichen Zhang,Jianghua Li,Long Liu,Jun Sun,Zhaozhe Hua,Guocheng Du,Jian Chen 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.1
The study aimed to produce 2-O-α-D-glucopyranosyl-L-ascorbic acid (AA-2G) via the transglycosylation reaction by α-cyclodextrin glucanotransferase (α-CGTase) from recombinant Escherichia coli with L-ascorbic acid (AA) and β-cyclodextrin (β-CD) as the substrates. Liquid chromatography-tandem mass spectrometry analysis was conducted for AA-2G identification, and the glucoamylase treatment was carried out to produce AA-2G from AA-2-oilgosaccharides. The optimal temperature and pH for the enzymatic AA-2G production were 37°C and 5.5,respectively, and the optimal α-CGTase concentration and substrate mass ratio (AA:β-CD) for AA-2G synthesis were 160 U/mL and 1:1, respectively. At these optimal process conditions, maximal AA-2G production reached 13 g/L. This is the first report regarding the process optimization of enzymatic AA-2G production by α-CGTase from recombinant E. coli. The results may be useful for the industrial scale production of AA-2G.
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Identification of DNA methylation and genetic alteration simultaneously from a single blood biopsy
Chen Xiaomin,Liu Jiahui,Li Jun,Xie Yinpeng,Yu Zichen,Shen Lu,Liu Qingfeng,Wu Wei,Zhao Qiang,Lin Haoxiang,Liu Gaotong,Luo Qiuping,Yang Ling,Huang Yi,Zhao Meiru,Yi Xin,Xia Xuefeng 한국유전학회 2023 Genes & Genomics Vol.45 No.5
Background High-throughput sequencing of blood cell-free DNA (cfDNA) techniques offer an opportunity to characterize and monitor cancer rapidly in a non-invasive and real-time manner. Nonetheless, there lacks a tool within therapeutic arsenal to identify multi-omics alterations simultaneously from a single biopsy. In current times, bisulfite-based sequencing detects 5mC and 5hmC at single-base resolution is the golden standard of DNA methylation, while the degradation of DNA and biased sequencing data are the problems of this method. Objective To identify the consistency analysis of methylation and genetic variation with single library, we presented a platform detecting multi-omics data simultaneously from a single blood biopsy using bisulfite-free method of genomic methylation sequencing (GM-seq) mediated by TET enzyme. Methods We detected methylomic and genetic changes simultaneously from a single blood biopsy in NA12878 and randomly chose ten blood biopsies from colorectal cancer or lung cancer patients to validate the ability of GM-seq. Results Similar cytosine methylation level between whole genome bisulfite sequencing (WGBS) and GM-seq were identified in NA12878. Moreover, longer insert size, CpGs coverage and GC distribution were outperformed than WGBS. In addition, the comparison of the single nucleotide polymorphism (SNP), insertion-deletion (Indel) and copy number variation (CNV) in NA12878 or ctDNA from liver cancer between GM-seq and whole genome sequencing (WGS) show a good consistency, indicating that this method is feasible for detecting genetic variation in blood. Conclusion In conclusion, our work demonstrated a method for identification of the methylated modification and genetic variations simultaneously from a single blood biopsy.
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Measure Synchronization in a Huygens’s Non-Dissipative Two-Pendulum Clocks System
Jing Tian,ZiChen Chen,HaiBo Qiu,XiaoQiang Xi 한국물리학회 2018 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.72 No.2
In this paper, we characterize measure synchronization (MS) in a four-degrees-of-freedom Huygens’s two-pendulum clocks system. The two-pendulum clocks are connected by a massless spring with stiffness constant k. We find that with the stiffness constant k increasing, the coupled pendulums system achieves MS above a threshold value of kc. The energy characteristics of measure synchronization have been discussed, it is found that averaged energy of each pendulum system provide us an easy way to characterize MS transition. Furthermore, we discuss the dependence of the critical value for MS transition on initial conditions and the characteristic parameters of the system.
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Experiment investigation on flow characteristics of open natural circulation system
Xiangjie Qi,Zichen Zhao,Peng Ai,Peng Chen,Zhongning Sun,Zhaoming Meng 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.5
Experimental research on flow characteristics of open natural circulation system was performed, tofigure out the mechanism of the open natural circulation behaviors. The influence factors, such as theheating power, the inlet subcooled and the level of cooling tank on the flow characteristics of the systemwere examined. It was shown that within the scope of the experimental conditions, there are five flowtypes: single-phase stable flow, flash and geyser coexisting unstable flow, flash stable flow, flash unstableflow, and flash and boiling coexisting unstable flow. The geyser flow in flash and geyser coexisting unstable flow is different from classic geysers flow. The flow oscillation period and amplitude of the formerare more regular, is a newly discovered flow pattern. By drawing the flow instability boundary diagramand sorting out the flow types, it is found that the two-phase unstable flow is mainly characterized byboiling and flash, which determine the behavior of open natural circulation respectively or jointly. Moreover, compared with full liquid level system, non-full liquid level system is more prone to boilingphenomenon, and the range of heat flux density and undercooling degree corresponding to unstableflow is larger
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Grey fuzzy PI control for packing pressure during injection molding process
Shuo Wang,Ji Ying,Zichen Chen,Kan Cai 대한기계학회 2011 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.25 No.4
This study presents a grey fuzzy PI (GFPI) controller for packing pressure control during the injection process. The novel controller was designed for solving the problems of large overshoot, static error, and long delay time on servo motor-driven injection molding machines. Based on reasonable assumptions, a mathematical model of injection system was established. According to the nonlinear, severe interferences, and long delay time characteristics in injection process, this paper integrates predictive grey system, robust fuzzy ratiocination,and PI control. Using these mathematical model and control algorithms, a GFPI controller is implemented into an MCU using C programming techniques. The integral discrete PID controller and solid fuzzy controller were realized for contrast experiments. The experimental results show that GFPI controller had better performance on reducing overshoot and static error, increasing both response rate and repeatable accuracy. Such a developed technology would provide helpful references for designing the controller of energy-saving servo motor-driven injection molding equipment.
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Machining Error Inspection of T-Spline Surface by On- Machine Measurement
Jintao Lai,Jianzhong Fu,Hongyao Shen,Wenfeng Gan,Zichen Chen 한국정밀공학회 2015 International Journal of Precision Engineering and Vol. No.
A new method for machining error inspection of T-spline surface by on-machine measurement (OMM) is investigated in this study. With the presented method, a relatively small number of sampling points are needed to evaluate the machining error of the surface. The sampling strategy based on the dominant of the control vertex is proposed. Based on the inspection data, the machined surfacecan be obtained through a T-spline surface reconstruction algorithm. Verification experiments showed that the inspection errorbetween the OMM method and the coordinate measuring machine (CMM) method is within 13.3%. Simulation results shows that thefitting error in critical area can be improved with less additional sampling points while the surface is presented in T-spline surface.
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