SET8 suppression mediates high glucose-induced vascular endothelial inflammation via the upregulation of PTEN

Shen Xuefang,Chen Xiangyuan,Wang Jing,Liu Jing,Wang Zhiyao,Hua Qing,Wu Qichao,Su Yanguang,He Huanzhong,Hu Yuqin,Meng Zhipeng,Xiong Wanxia,Zhu Minmin 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-

Hyperglycemia-mediated endothelial inflammation participates in the pathogenesis of cardiovascular complications in subjects with diabetes. Previous studies reported that phosphatase and tensin homolog deleted on chromosome ten (PTEN) and SET8 participate in high glucose-mediated endothelial inflammation. In this study, we hypothesize that SET8 regulates PTEN expression, thus contributing to high glucose-mediated vascular endothelial inflammation. Our data indicated that plasma soluble intercellular adhesion molecule-1 (sICAM-1) and endothelial selectin (e-selectin) were increased in patients with diabetes and diabetic rats. PTEN expression was augmented in the peripheral blood mononuclear cells of patients with diabetes and in the aortic tissues of diabetic rats. Our in vitro study indicated that high glucose increased monocyte/endothelial adhesion, endothelial adhesion molecule expression and p65 phosphorylation in human umbilical vein endothelial cells (HUVECs). Moreover, high glucose led to endothelial inflammation via upregulation of PTEN. Furthermore, high glucose inhibited SET8 expression and histone H4 lysine 20 methylation (H4K20me1), a downstream target of SET8. SET8 overexpression reversed the effects of high-glucose treatment. shSET8-mediated endothelial inflammation was counteracted by siPTEN. Furthermore, SET8 was found to interact with FOXO1. siFOXO1 attenuated high glucose-mediated endothelial inflammation. FOXO1 overexpression-mediated endothelial inflammation was counteracted by siPTEN. H4K20me1 and FOXO1 were enriched in the PTEN promoter region. shSET8 increased PTEN promoter activity and augmented the positive effect of FOXO1 overexpression on PTEN promoter activity. Our in vivo study indicated that SET8 was downregulated and FOXO1 was upregulated in the peripheral blood mononuclear cells of patients with diabetes and the aortic tissues of diabetic rats. In conclusion, SET8 interacted with FOXO1 to modulate PTEN expression in vascular endothelial cells, thus contributing to hyperglycemia-mediated endothelial inflammation.

PP-TABLF Based Adaptive Command Filter Control for Fully Actuated MSVs with Unknown Uncertainties and Multiple Constraints

Yannan Bi,Zhipeng Shen,Qun Wang,Haomiao Yu,Chen Guo 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.12

This article investigates the adaptive tracking control problem for the marine surface vessels (MSVs) with unknown uncertainties and multiple constraints. Firstly, a novel prescribed performance-based time-varying asystematic barrier Lyapunov function (PP-TABLF) is proposed to control the MSVs to navigate in a variable narrow waterway and to improve the transient performance of MSVs. Secondly, the predictor-based radial basis function neural networks (P-RBFNNs) are developed to approximate the system uncertainties and external disturbances. Specifically, the tracking errors of the RBFNNs are estimated in advance, and the prediction errors are utilized to update the RBFNNs and improve the estimation precision. The command filter and the idea of the recursive sliding mode are integrated with the control law to limit the amplitude of the virtual control signals and to reduce the computational burden. With the proposed control scheme, tracking errors will not override the prescribed performance ranges, and the control force will not be violated in the presence of total unknown uncertainties. Finally, the semi-global uniformly ultimate boundedness of the system is guaranteed by the proposed control scheme, and the simulation results are given to further demonstrate the effectiveness of the proposed approach.

Aldol condensation of acetic acid with formaldehyde to acrylic acid over SiO2-, SBA-15-, and HZSM-5-supported V-P-O catalysts

Jing Hua,Zhipeng Lu,Hengbo Yin,Wuping Xue,Aili Wang,Lingqin Shen,Shuxin Liu 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.40 No.-

Aldol condensation of acetic acid with formaldehyde to acrylic acid was catalyzed by the SiO2-, SBA-15-,and HZSM-5-supported vanadium phosphorous oxide (V-P-O) catalysts prepared by the incipientwetness impregnation. The supports obviously increased the surface areas and changed the acid–baseproperties of the as-prepared catalysts. V-P-O/SBA-15 catalysts with high acid and alkali quantitiesexhibited high catalytic activities for the aldol condensation of acetic acid with formaldehyde to acrylicacid. When the V-P-O/SBA-15 catalyst with the P/V mole ratio of 2.0:1 catalyzed the reaction at 330–370 8C, the acrylic acid selectivities were 90.8–70.2% at the formaldehyde conversions of 14.3–68.7%.

The Construction of the Animal Husbandry Information System Based on the Technology of Map Conflation

Yue Guo,Zhongbin Su,Weizheng Shen,Zhipeng Guo,Qingming Kong 보안공학연구지원센터 2015 International Journal of Smart Home Vol.9 No.3

The research aims to merge the sections of geographic information distribution of the large-scale farms information monitoring system and the farming enterprises filing system which are under the Animal Husbandry Bureau in Heilongjiang Province as a Geographic Information System (GIS) that based on the map conflation technology of topological relation. Applying a variety of algorithms of points, lines, surfaces to this study, and using optimized "Spider code" and matching algorithm based on area overlay rate to solve the map database conflation problem of two different sources but consistent geographic target. It not only improves the map accuracy and consistency, but also adds new space characteristics, and updates attribute information which associated with dataset spatial characteristics. It makes the personnel at all levels in the Animal Husbandry Bureau in Heilongjiang Province realize information sharing. It is conductive to promote the work of staff at all levels and provide decision support of data to the Animal Husbandry Bureau in Heilongjiang Province.

Reliability-based Event Driven Backstepping Positioning Control for a Turret-moored FPSO Vessel with Unknown Slow Time-varying Disturbances

Yulong Tuo,ShashaWang,Chen Guo,Haomiao Yu,Zhipeng Shen 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.2

In this paper, an event driven backstepping positioning controller based on the structural reliability is developed for a turret-moored floating production storage and offloading (FPSO) vessel with unknown slow timevarying disturbances. The mathematical model of the FPSO vessel is given at first. Secondly, to make full use of the positioning capability of mooring system while ensuring the safety of mooring lines, a continuous backstepping positioning controller is designed based on the structural reliability of the mooring system; in the meanwhile, a disturbance observer is designed to handle the unknown disturbances. Then, the proposed event driven positioning controller is achieved based on the designed continuous controller by designing suitable event driven conditions, which can determine the updated time instants of the event driven positioning controller. The proposed positioning controller can not only guarantee the uniform ultimate boundness of the errors for structural reliability and heading but also ensure that no Zeno behavior occurs. Finally, the simulations demonstrate that the proposed event driven positioning controller’s performance while reducing the execution times of FPSO vessel’s actuators.

Structural and functional properties of Maillard-reacted casein phosphopeptides with different carbohydrates

Meng Yuan,Yu Cao,Haoyang Zheng,Kunlin Chen,Yuping Lu,Jing Wang,Liqin Zhu,Ming Chen,Zhipeng Cai,Yonggen Shen 한국식품과학회 2024 Food Science and Biotechnology Vol.33 No.7

This study used glucose, fructose, maltose and dextran to explore the effects of different carbohydrates on the Maillard reaction of casein phosphopeptides (CPP). The color parameter results showed that heating time from 1 to 5 h led to brown color, which was consistent with the observed increased in browning intensity. Fourier transform infrared spectroscopy results verified that four carbohydrates reacted with CPP to produce Maillard conjugates. Fluorescence spectroscopy showed that the Maillard reaction changed the tertiary structure of CPP by decreasing the intrinsic fluorescence intensity and surface hydrophobicity compared with the CPP-carbohydrate mixture. At the same time, the Maillard reaction effectively improved the emulsifying properties, reducing power and DPPH radical scavenging activity of CPP. Furthermore, this study also found that glucose and fructose improved CPP more than maltose and dextran. Therefore, monosaccharides have good potential in modifying CPP via the Maillard reaction.