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Characterization of a strong green tissue-specific motif in rice photosystem I gene promoter Ppsak
Zhimin Lin,Jingwan Yan,Huanxin Yan,Feng Wang 한국식물생명공학회 2017 Plant biotechnology reports Vol.11 No.2
Characterization of green tissue-specific promoters helps facilitate genetic improvement in crops. Here, we isolated a novel green tissue-specific expression gene Psak encoding photosystem I protein in rice through RTPCR analysis. The 5′ flanking region from −635 to +60 (transcription start site of Psak as +1) covering parts of Psak gene and its promoter Ppsak was identified as be critical for green tissue-specific expression (including leaves and stems) in rice. Further promoter deletion analyses demonstrated that the promoter region from −559 to −232 was necessary and sufficient for green tissue-specific expression of Psak gene. Histochemical assays showed that the GUS expression of this 267-bp region was highly presented in leaves and stems, but reduced or absent in other tissues examined. The GUS expression level of this core promoter region could be 14.6- to 21.6-fold higher than that of the full-length region of Ppsak promoter in leaves and stems. These results combined with cis-acting elements prediction in Ppsak promoter suggested that the TCT motif located in this promoter played a key role in strong green tissue-specific expression.
On Privacy and Anonymity in Freenet System
Tianbo Lu,Zhimin Lin,Lingling Zhao,Yang Li 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.5
As a typical representative of anonymous network applications, Freenet system has so many advantages in sharing, privacy, anonymity as well as uploading and downloading convenience that it adequately realizes expression freedom. So it’s widely used in different fields. For Freenet system, academia and system designers have been conducting research and improvement which mainly on following points: structure, topology, routing algorithm and the Darknet mechanism which proposed by new version. The purpose of these efforts is to increase the system network utilization, enhance the reliability of transmission and improve the safety and robustness of the system. This paper reviews and summarizes the research progress of Freenet system by collating and analyzing relevant articles. We also analyze and compare the main ideas, the algorithm application as well as pros and cons of different articles surrounding different topics. In addition, we also tease out the development and the evolution trends of Freenet system in time order, and combined with current network situation, we made reasonable proposals and prospects and draw scientific conclusions.
Yan Jingwan,Lin Zhimin,Hu Changquan,Wang Feng 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.6
A new phosphatidylinositol transporter gene was isolated from the T-DNA insertion mutant libraries of rice. The RT-PCR and GUS staining showed that it belonged to embryo specific expression gene. The phonotype of growth and development revealed that the growth was slower and leaf yellowing than that of wild type before 5-leaf stage in rice seedlings, and it was completely restored until to 5-leaf stage. Further, the transcriptome sequencing results showed that the gene affected the nitrogen metabolism signal pathway between mutant and wild type. In addition, our study revealed an important phosphati- dylinositol transporter protein which can control the growth development and promote nitrate transporter and absorption in rice seedling stage.
Cluster-Based Polarized Spectrum Sharing in Channels with Polarization Mode Dispersion
Dongming Li,Zhimin Zeng,Caili Guo,Xiaolin Lin 한국전자통신연구원 2014 ETRI Journal Vol.36 No.3
Polarized spectrum sharing (PSS) exploits the spectrumopportunities in a polarized domain. However, when it comesto wideband environments PSS is impaired by the frequencydependentpolarization mode dispersion (PMD); thus, theeffective throughput of PSS drops. To combat the PMD effect,this work proposes a cluster-based PSS approach to enablePSS on a narrower frequency span. Simulation results showthat the effective throughput of PSS on cluster basisoutperforms that of PSS on bandwidth and subcarrier basis.
( Ahmed E. Gomaa ),( Zhiping Deng ),( Zhimin Yang ),( Liguo Shang ),( Yuhua Zhan ),( Wei Lu ),( Min Lin ),( Yongliang Yan ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.2
The complexity of the bacterial recombination system is a barrier for the construction of bacterial mutants for the further functional investigation of specific genes. Several protocols have been developed to inactivate genes from the genus Pseudomonas. Those protocols are complicated and time-consuming and mostly do not enable easy construction of multiple knock-ins/outs. The current study describes a single and double crossover-recombination system using an optimized vector-free allele-exchange protocol for gene disruption and gene replacement in a single species of the family Pseudomonadaceae. The protocol is based on selfligation (circularization) for the DNA cassette which has been obtained by overlapping polymerase chain reaction (Fusion-PCR), and carries an antibiotic resistance cassette flanked by homologous internal regions of the target locus. To establish the reproducibility of the approach, three different chromosomal genes (ncRNA31, rpoN, rpoS) were knocked-out from the root-associative bacterium Pseudomonas stutzeri A1501. The results showed that the P. stutzeri A1501 mutants, which are free of any plasmid backbone, could be obtained via a single or double crossover recombination. In order to optimize this protocol, three key factors that were found to have great effect on the efficiency of the homologous recombination were further investigated. Moreover, the modified protocol does not require further cloning steps, and it enables the construction of multiple gene knock-in/out mutants sequentially. This work provides a simple and rapid mutagenesis strategy for genome editing in P. stutzeri, which may also be applicable for other gram-negative bacteria.
Ze-Hua Zhao,Feng-Zhi Xin,Yaqian Xue,Zhimin Hu,Yamei Han,Fengguang Ma,Da Zhou,Xiao-Lin Liu,Aoyuan Cui,Zhengshuai Liu,Yuxiao Liu,Jing Gao,Qin Pan,Yu Li,Jian-Gao Fan 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-
Microbial metabolites have emerged as critical components that mediate the metabolic effects of the gut microbiota. Here, we show that indole-3-propionic acid (IPA), a tryptophan metabolite produced by gut bacteria, is a potent anti-non-alcoholic steatohepatitis (NASH) microbial metabolite. Here, we demonstrate that administration of IPA modulates the microbiota composition in the gut and inhibits microbial dysbiosis in rats fed a high-fat diet. IPA induces the expression of tight junction proteins, such as ZO-1 and Occludin, and maintains intestinal epithelium homeostasis, leading to a reduction in plasma endotoxin levels. Interestingly, IPA inhibits NF-κB signaling and reduces the levels of proinflammatory cytokines, such as TNFα, IL-1β, and IL-6, in response to endotoxin in macrophages to repress hepatic inflammation and liver injury. Moreover, IPA is sufficient to inhibit the expression of fibrogenic and collagen genes and attenuate diet-induced NASH phenotypes. The beneficial effects of IPA on the liver are likely mediated through inhibiting the production of endotoxin in the gut. These findings suggest a protective role of IPA in the control of metabolism and uncover the gut microbiome and liver cross-talk in regulating the intestinal microenvironment and liver pathology via a novel dietary nutrient metabolite. IPA may provide a new therapeutic strategy for treating NASH.