Enhanced Supercapacitive Performance of Carbonized Polyaniline by Conducting Wrapping

Zhihong Luo,Xiangqun Zhuge,Yuzhen Zhao,Meixiao He,Lihua Zhu,Heiqing Tang,Kun Luo 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.8

Functionalized carbon nanotubes (MWNT–COOH) was selected as conducting wrapping agent to prepare composites of MWNT–COOH and carbonized polyaniline (C–PANI), to improve the supercapacitive performance of C–PANI. The morphology was characterized by scanning electron microscope and transmission electron microscope which showed that C–PANI was trapped in the conductive network of MWNT–COOH. The electrochemical measurement results indicated the increased specific capacitance and enhanced cycling stability of C–PANI with the addition of MWNT–COOH. When the weight ratio of C–PANI and MWNT–COOH was 4:1, the specific capacitance of the composites was 149 F g-1, and the capacitance retention was 93.7% after 1000 charge–discharge cycling.

Identification and Validation of Potential Conserved microRNAs and Their Targets in Peach (Prunus persica)

Zhihong Gao,Xiaoyan Luo,Ting Shi,Bin Cai,Zhen Zhang,Zongming Cheng,Weibing Zhuang 한국분자세포생물학회 2012 Molecules and cells Vol.34 No.3

MicroRNAs are a class of small, endogenous, non-coding RNA molecules that negatively regulate gene expression at the transcriptional or the post-transcriptional level. Although a large number of miRNAs have been identified in many plant species, especially from model plants and crops, they remain largely unknown in peach. In this study, 110 potential miRNAs belonging to 37 families were identified using computational methods. A total of 43 potential targets were found for 21 families based on near-perfect or perfect complementarity between the plant miRNA and the target sequences. A majority of the targets were transcription factors which play important roles in peach development. qRT-PCR analysis of RNA samples prepared from different peach tissues for 25 miRNA families revealed that miRNAs were differentially expressed in different tissues. Furthermore, two target genes were experimentally verified by detection of the miRNA-mediated mRNA cleavage sites in peach using RNA ligase-mediated 5’ rapid amplification of cDNA ends (RLM-RACE). Finally, we studied the expression pattern of the two target genes in three different tissues of peach to further understand the mechanism of the interaction between miRNAs and their target genes.

A Bacillus coagulans and its Application in Pine Pollen Fermentation

Shanyan Yang,Yaoyao Wu,Chubo Luo,Zhihong Di,Yunpeng Wu,Jie Zhang,Yaomei Xu 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.6

In the study, a potential L-lactic acid-producing bacillus-strain LT1 was isolated. It was identified as Bacillus coagulans based on its morphological, physiological, biochemical properties, and phylogenetic similarity of 16S rDNA. The fermentation conditions of pine pollen by strain LT1 were optimized using Box-Behnken design and response surface methodology. Optimum values of the tested variables for production of viable counts were an inoculum size of 9.22%, temperature= 49.21℃, and an initial pH of 6.82. Under optimized conditions, the viable count was 5.49×109 CFU/mL. Finally, the single factor experiment as preliminary research for fermentation product treatment was performed.

Numerical investigation of cavitation-vortex interaction in a mixed-flow waterjet pump

Renfang Huang,Bin Ji,Xianwu Luo,Zhihong Zhai,Jiajian Zhou 대한기계학회 2015 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.29 No.9

Turbulent cavitating flows in a mixed-flow waterjet pump were numerically investigated using the k-w SST turbulence model and themass transfer cavitation model based on the Rayleigh-Plesset equation to provide a comprehensive understanding of the cavitation-vortexinteraction mechanism. The predicted hydraulic performance, as well as the cavitation performance, exhibits a reasonable agreement withthe experimental results. The vorticity distributions under three operation conditions were illustrated together. Based on the illustration,cavitation development enhances vorticity production and flow unsteadiness in a mixed-flow waterjet pump. Vortices are basically locatedat the cavity interface, particularly at the downstream interface, during cavitation. Further analyses using the relative vorticity transportequation in cavitating turbulent flows indicate that vortex dilation and baroclinic torque exhibit a steep jump as cavitation occurs. Inaddition, vortex stretching contributes mainly to large-scale vortex generation.

Intermetallic Compounds (IMCs) Formation in Al/Mg Dissimilar Alloy Solid State Joining: Experimental Study and Phase Field Modeling

Kuijing Song,Xinquan Liu,Yukai Ji,Yong Wei,Junrui Luo,Fei Liu,Dashuang Liu,Zhihong Zhong 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.4

Aluminum/magnesium (Al/Mg) alloys have good metallurgical compatibility as well as similar melting and recrystallizationtemperatures. Therefore, the formation of deleterious IMCs is considered as a major problem in dissimilar Al/Mgalloys joining. In this paper, the metallurgical reaction behavior and atomic diffusion in Al/Mg solid state joining interfaceare studied. Based on the thermodynamic, kinetic and crystallographic characteristics of ordered phase transformation andatomic diffusion database, a phase field calculation framework of the polycrystalline β–Al3Mg2 and γ–Al12Mg17 evolutionis developed. Diffusivity, D and interface mobility, M, which significant kinetic parameters are defined as alloy compositionsand joining conditions dependent. By a series of simulation, it is proved the simulated interdiffusion microstructuralcharacteristics and element distribution across the joining interface are in coincidence with the corresponding experimentaldata. The thermally activated IMC evolution in diffusion bonding is in thermodynamic equilibrium, while accelerated IMCevolution in friction stir welding results from thermomechanically activated D and M. The present study contributes to theunderstanding of interfacial microstructures evolution in solid state joining of dissimilar alloys.

Lnc Tmem235 promotes repair of early steroid-induced osteonecrosis of the femoral head by inhibiting hypoxia-induced apoptosis of BMSCs

Zhang Fei,Peng Wuxun,Wang Tao,Zhang Jian,Dong Wentao,Wang Chuan,Xie Zhihong,Luo Hong,Liu Gang 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Bone marrow mesenchymal stem cells (BMSCs) have been used in the treatment of early steroid-induced osteonecrosis of the femoral head (SONFH). However, the hypoxic microenvironment in the osteonecrotic area leads to hypoxia-induced apoptosis of transplanted BMSCs, which limits their efficacy. Therefore, approaches that inhibit hypoxia-induced apoptosis of BMSCs are promising for augmenting the efficacy of BMSC transplantation. Our present study found that under hypoxia, the expression of the long noncoding RNA (Lnc) transmembrane protein 235 (Tmem235) was downregulated, the expression of Bcl-2-associated X protein was upregulated, the expression of B-cell lymphoma-2 protein was downregulated, and the apoptotic rate of BMSCs was over 70%. However, overexpression of Lnc Tmem235 reversed hypoxia-induced apoptosis of BMSCs and promoted their survival. These results demonstrated that Lnc Tmem235 effectively inhibited hypoxia-induced apoptosis of BMSCs. Mechanistically, we found that Lnc Tmem235 exhibited competitive binding to miR-34a-3p compared with BIRC5 mRNA, which is an inhibitor of apoptosis; this competitive binding relieved the silencing effect of miR-34a-3p on BIRC5 mRNA to ultimately inhibit hypoxia-induced apoptosis of BMSCs by promoting the expression of BIRC5. Furthermore, we cocultured BMSCs overexpressing Lnc Tmem235 with xenogeneic antigen-extracted cancellous bone to construct tissue-engineered bone to repair a model of early SONFH in vivo. The results showed that overexpression of Lnc Tmem235 effectively reduced apoptosis of BMSCs in the hypoxic microenvironment of osteonecrosis and improved the effect of BMSC transplantation. Taken together, our findings show that Lnc Tmem235 inhibited hypoxia-induced apoptosis of BMSCs by regulating the miR-34a-3p/BIRC5 axis, thus improving the transplantation efficacy of BMSCs for treating early SONFH.