Clinical Application of Transcatheter Arterial Chemoembolization Combined with Synchronous C-arm Cone-Beam CT Guided Radiofrequency Ablation in treatment of Large Hepatocellular Carcinoma

Wang, Zhi-Jun,Wang, Mao-Qiang,Duan, Feng,Song, Peng,Liu, Feng-Yong,Wang, Yan,Yan, Jie-Yu,Li, Kai,Yuan, Kai Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

Objective: This work aimed to evaluate the safety and clinical efficacy of transcatheter arterial chemoembolization (TACE) combined with c-arm cone-beam CT guided synchronous radiofrequency ablation (RFA) in treatment of large hepatocellular carcinoma (HCC). Methods: 21 patients with large HCC were studied from January 2010 to March 2012. TACE combined with synchronous C-arm cone-beam CT guided RFA were performed on a total of 25 lesions. Conventional imaging examination (CEUS, enhanced CT or MRI) and AFP detection were regularly conducted to evaluate the technical success rate of combined treatment, complications, treatment response, time without disease recurrence and survival rate. Results: The technical success rate of combined treatment was 100%, without any significant complication. After 1 month, there were 19 cases with complete response and 2 cases with partial response, with an complete response rate of 90.4% (19/21) and a clinical effective rate of 100% (21/21). The complete response rates of single nodular lesions (100%, 17/17) was significantly higher than that of multiple nodular lesions (50%, 2/4) (P<0. 05). During 2 to 28 months of follow-up, in 19 cases with complete response, the average time without disease recurrence was $10.8{\pm}6$ months. The total survival rates of 6, 12 and 18 months in 21 patients were 100%, respectively. Conclusion: TACE combined with synchronous C-arm CT guided RFA is safe and effective for treatment of large HCC. The treatment efficacy for single nodular lesion is better than that for multiple nodular lesions.

Experimental Analysis of Equilibrization in Binary Classification for Non-Image Imbalanced Data Using Wasserstein GAN

Zhi-Yong Wang,강대기 한국인터넷방송통신학회 2019 International Journal of Internet, Broadcasting an Vol.11 No.4

In this paper, we explore the details of three classic data augmentation methods and two generative model based oversampling methods. The three classic data augmentation methods are random sampling (RANDOM), Synthetic Minority Over-sampling Technique (SMOTE), and Adaptive Synthetic Sampling (ADASYN). The two generative model based oversampling methods are Conditional Generative Adversarial Network (CGAN) and Wasserstein Generative Adversarial Network (WGAN). In imbalanced data, the whole instances are divided into majority class and minority class, where majority class occupies most of the instances in the training set and minority class only includes a few instances. Generative models have their own advantages when they are used to generate more plausible samples referring to the distribution of the minority class. We also adopt CGAN to compare the data augmentation performance with other methods. The experimental results show that WGAN-based oversampling technique is more stable than other approaches (RANDOM, SMOTE, ADASYN and CGAN) even with the very limited training datasets. However, when the imbalanced ratio is too small, generative model based approaches cannot achieve satisfying performance than the conventional data augmentation techniques. These results suggest us one of future research directions.

Long noncoding RNA DANCR promotes colorectal cancer proliferation and metastasis via miR-577 sponging

Yong Wang,Zhi Lu,Ningnin Wang,Jianzhou Feng,Junjie Zhang,Lan Luan,Wei Zhao,Xiandong Zeng 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Long non-coding RNAs (lncRNAs) play key roles in various malignant tumors, including colorectal cancer (CRC). Long non-coding RNA differentiation antagonizing non-protein coding RNA (DANCR) is overexpressed in CRC patients, but whether it affects CRC proliferation and metastasis via regulation of heat shock protein 27 (HSP27) remains unclear. In the present study, we found that DANCR was highly expressed and correlated with proliferation and metastasis in CRC. In addition, we demonstrated that DANCR and HSP27 were both targets of microRNA-577 (miR-577) and shared the same binding site. Furthermore, we revealed that DANCR promoted HSP27 expression and its mediation of proliferation/metastasis via miR-577 sponging. Finally, using an in vivo study, we confirmed that overexpression of DANCR promoted CRC tumor growth and liver metastasis. The present study demonstrated the function of DANCR in CRC and might provide a new target in the treatment of CRC.

Symposia : Plant Genetics ; Brassinosteroid signal transduction - molecular genetics studies and beyond

( Zhi Yong Wang ) 한국생화학분자생물학회 (구 한국생화학회) 2007 생화학분자생물학회 춘계학술발표논문집 Vol.2007 No.-

Wide-Tunable Mid Infrared Intra-cavity Optical Parametric Oscillator Based on Multi-period MgO:PPLN

Xiao-Chan Wang,Yu-Heng Wang,Hao Zheng,Hong-Zhi Liu,Yong-Ji Yu,Zi-Jian Wang 한국광학회 2021 Current Optics and Photonics Vol.5 No.1

This paper reports a tunable diode-pumped folded intracavity Q-switched singly resonant optical parametric oscillator based on multi-period MgO:PPLN. A wide tuning mid-infrared parametric lightfrom 2.78 μm to 4.17 μm was obtained in real time by changing the poled periods and temperatures. The maximum output power of 1.89 W at 3.2 μm, 1.53 W at 3.5 μm, 0.87 W at 3.8 μm and 0.486 W at 4.1 μm were achieved. The highest optical-optical conversion efficiency was 7.89%. During experiments, a range tunable output of 2.78–4.17 μm in the mid-infrared range was achieved.

Transmembrane Helix of Novel Oncogene with Kinase-Domain (NOK) Influences Its Oligomerization and Limits the Activation of RAS/MAPK Signaling

Ying-Hua Li,Yin-Yin Wang,Shan Zhong,Zhi-Li Rong,Yong-Ming Ren,Zhi-Yong Li,Shu-Ping Zhang,Zhi-Jie Chang,Li Liu 한국분자세포생물학회 2009 Molecules and cells Vol.27 No.1

Ligand-dependent or independent oligomerization of receptor protein tyrosine kinase (RPTK) is often an essential step for receptor activation and intracellular signaling. The novel oncogene with kinase-domain (NOK) is a unique RPTK that almost completely lacks an ectodomain, expresses intracellularly and activates constitutively. However, it is unknown whether NOK can form oligomer or what function oligomerization would have. In this study, two NOK deletion mutants were generated by either removing the ectodomain (NOKECD) or including the endodomain (NOK-ICD). Co-immunoprecipitation demonstrated that the transmembrane (TM) domain of NOK was essential for its intermolecular interaction. The results further showed that NOK aggregated more closely as lower order oligomers (the dimer- and trimer-sized) than either deletion mutant did since NOK could be cross-linked by both Sulfo-EGS and formaldehyde, whereas either deletion mutant was only sensitive to Sulfo-EGS. Removing the NOK TM domain (NOK-ICD) not only markedly promoted higher order oligomerization, but also altered the subcellular localization of NOK and dramatically elevated the NOK-mediated constitutive activation of extracellular signal-regulated kinase (ERK). Moreover, NOK-ICD but not NOK or NOKECD was co-localized with the upstream signaling molecule RAS on cell membrane. Thus, TM-mediated intermolecular contacting may be mainly responsible for the constitutive activation of NOK and contribute to the autoinhibitory effect on RAS/MAPK signaling.

Perimeter Intrusion Detection Based on Intelligent Video Analysis

Yong-Liang ZHANG,Zhi-Qin ZHANG,Gang XIAO,Rui-Dong WANG,Xia HE 제어로봇시스템학회 2015 제어로봇시스템학회 국제학술대회 논문집 Vol.2015 No.10

Monitoring system has become one of the most important means for perimeter intrusion prevention. But most of existing monitoring systems are passive surveillance. In this paper, we propose a method to implement active perimeter intrusion detection by identifying human targets in video images captured by monitoring system. In order to enhance the robustness of detecting postures of human targets, this paper introduces Fourier Descriptor (FD) and Histogram of Oriented Gradients (HOG) to realize an effective detection of human bodies with multiple postures captured by fixed cameras. The experiment results confirm that the proposed algorithm has higher recognition rate for detecting human targets with walking, climbing, and jumping postures, and sufficiently meets the requirements of perimeter intrusion detection.

Diversity of Endophytic Fungi from Different Verticillium-Wilt-Resistant Gossypium hirsutum and Evaluation of Antifungal Activity Against Verticillium dahliae In Vitro

( Zhi Fang Li ),( Ling Fei Wang ),( Zi Li Feng ),( Li Hong Zhao ),( Yong Qiang Shi ),( He Qin Zhu ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.9

Cotton plants were sampled and ranked according to their resistance to Verticillium wilt. In total, 642 endophytic fungi isolates representing 27 genera were recovered from Gossypium hirsutum root, stem, and leaf tissues, but were not uniformly distributed. More endophytic fungi appeared in the leaf (391) compared with the root (140) and stem (111) sections. However, no significant difference in the abundance of isolated endophytes was found among resistant cotton varieties. Alternaria exhibited the highest colonization frequency (7.9%), followed by Acremonium (6.6%) and Penicillium (4.8%). Unlike tolerant varieties, resistant and susceptible ones had similar endophytic fungal population compositions. In three Verticillium-wilt-resistant cotton varieties, fungal endophytes from the genus Alternaria were most frequently isolated, followed by Gibberella and Penicillium. The maximum concentration of dominant endophytic fungi was observed in leaf tissues (0.1797). The evenness of stem tissue endophytic communities (0.702) was comparatively more uniform than the other two tissues. Eighty endophytic fungi selected from 27 genera were evaluated for their inhibition activity against highly virulent Verticillium dahliae isolate Vd080 in vitro. Thirty-nine isolates exhibited fungistasis against the pathogen at varying degrees. Seven species, having high growth inhibition rates (≥75%), exhibited strong antifungal activity against V. dahliae. The antifungal activity of both volatile and nonvolatile metabolites was also investigated. The nonvolatile substances produced by CEF-818 (Penicillium simplicissimum), CEF-325 (Fusarium solani), CEF-714 (Leptosphaeria sp.), and CEF-642 (Talaromyces flavus) completely inhibited V. dahliae growth. These findings deepen our understanding of cotton-endophyte interactions and provide a platform for screening G. hirsutum endophytes with biocontrol potential.

Proteomic analyses for profiling regulated proteins/enzymes by <i>Fucus vesiculosus</i> fucoidan in B16 melanoma cells: A combination of enzyme kinetics functional study

Wang, Zhi-Jiang,Zheng, Li,Yang, Jun-Mo,Kang, Yani,Park, Yong-Doo Elsevier 2018 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Vol.112 No.-

<P><B>Abstract</B></P> <P>Fucoidans are complex sulfated polysaccharides that have a wide range of biological activities. Previously, we reported the various effects of <I>Fucus vesiculosus</I> fucoidan on tyrosinase and B16 melanoma cells. In this study, to identify fucoidan-targeted proteins in B16 melanoma cells, we performed a proteomics study and integrated enzyme kinetics. We detected 19 candidate proteins dysregulated by fucoidan treatment. Among the probed proteins, the enzyme kinetics of two candidate enzymes, namely lactate dehydrogenase (LDH) as an upregulated protein and superoxide dismutase (SOD) as a downregulated enzyme, were determined. The enzyme kinetics results showed that <I>Fucus vesiculosus</I> fucoidan significantly inhibited LDH catalytic function while it did not affect SOD activity even at a high dose, while only slightly decreased activity (up to 10%) at a low dose. Based on our previous and present observations, fucoidan could inhibit B16 melanoma cells growth via regulating proteins/enzymes expression levels such as LDH and SOD known as cell survival biomarkers. Interestingly, both expression level and enzyme catalytic activity of LDH were regulated by fucoidan, which could directly induce the apoptotic effect on B16 melanoma cells along with SOD downregulation. This study highlights how combining proteomics with enzyme kinetics can yield valuable insights into fucoidan targets.</P>

Suppression of the Epidermal Growth Factor-like Domain 7 and Inhibition of Migration and Epithelial-Mesenchymal Transition in Human Pancreatic Cancer PANC-1 Cells

Wang, Yun-Liang,Dong, Feng-Lin,Yang, Jian,Li, Zhi,Zhi, Qiao-Ming,Zhao, Xin,Yang, Yong,Li, De-Chun,Shen, Xiao-Chun,Zhou, Jin Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9

Background: Epidermal growth factor-like domain multiple 7 (EGFL7), a secreted protein specifically expressed by endothelial cells during embryogenesis, recently was identified as a critical gene in tumor metastasis. Epithelial-mesenchymal transition (EMT) was found to be closely related with tumor progression. Accordingly, it is important to investigate the migration and EMT change after knock-down of EGFL7 gene expression in human pancreatic cancer cells. Materials and Methods: EGFL7 expression was firstly testified in 4 pancreatic cancer cell lines by real-time polymerase chain reaction (Real-time PCR) and western blot, and the highest expression of EGFL7 was found in PANC-1 cell line. Then, PANC-1 cells transfected with small interference RNA (siRNA) of EGFL7 using plasmid vector were named si-PANC-1, while transfected with negative control plasmid vector were called NC-PANC-1. Transwell assay was used to analyze the migration of PANC-1 cells. Real-time PCR and western blotting were used to detect the expression change of EGFL7 gene, EMT markers like E-Cadherin, N-Cadherin, Vimentin, Fibronectin and transcription factors like snail, slug in PANC-1, NCPANC-1, and si-PANC-1 cells, respectively. Results: After successful plasmid transfection, EGFL7 gene were dramatically knock-down by RNA interference in si-PANC-1 group. Meanwhile, migration ability decreased significantly, compared with PANC-1 and NC-PANC-1 group. Meanwhile, the expression of epithelial phenotype marker E-Cadherin increased and that of mesenchymal phenotype markers N-Cadherin, Vimentin, Fibronectin dramatically decreased in si-PANC-1 group, indicating a reversion of EMT. Also, transcription factors snail and slug decreased significantly after RNA interference. Conclusions: Current study suggested that highly-expressed EGFL7 promotes migration of PANC-1 cells and acts through transcription factors snail and slug to induce EMT, and further study is needed to confirm this issue.