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Catenary Equation-Based Approach for Force Finding of Cable Domes
Zhengrong Jiang,Xiaowei Liu,Kairong Shi,Quanpan Lin,Zijian Zhang 한국강구조학회 2019 International Journal of Steel Structures Vol.19 No.1
Cable forces along the cable length direction vary with deadweight. An invariant that is horizontal component force along the cable length, is introduced to characterize prestress distribution of cable domes. A new approach named catenary equationbased component force balancing method, is proposed for force fi nding. In the method, the relationship between the form and the horizontal component force of the cable is determined according to catenary equation of the cable. Based on the horizontal component force of one cable, the horizontal component forces of other cables can be solved from the nodal equilibrium relationship. Take the Geiger cable dome as an example, the results of force fi nding show that the proposed method has clear mechanical concept, high calculation accuracy and simple solving process. Thus, it can provide a new general idea for force fi nding of cable domes. In addition, the diff erence of the results of force fi nding is large between with deadweight considered and with deadweight ignored. Therefore, deadweight should be considered during the prestress design of cable domes.
Mo, Rongli,Zhang, Na,Yang, Sichao,Zhang, Qinglin,Luo, Zhengrong Korean Society of Horticultural Science 2016 원예과학기술지 Vol.34 No.2
A transient ihpRNA-induced gene silencing system based on Agrobacterium-mediated injection infiltration has been established to evaluate candidate genes involved in proanthocyanidin (PAs) biosynthesis in persimmon (Diospyros kaki Thunb.). We chose DkPDS (phytoene desaturase) as a gene-silencing target to evaluate the newly developed transient gene silencing system. Our qRT-PCR analysis indicated that two ihpRNA constructs (pHG-PDS5' and pHG-PDS3') targeted DkPDS, which also led to significantly reduce expression of DkPDS in 'Mopanshi' persimmon leaves. To further confirm the reliability of the system, we successfully utilized it for DkLAR (leucoanthocyanidin reductase) gene silencing. The expression levels of DkLAR in 'Mopanshi' and 'Eshi 1' leaves were ca. 6-fold and ca. 5-fold lower than those in leaves harboring empty vector (pHG-GFP), respectively. DMACA (4-dimethylaminocinnamaldehyde) staining and the Folin-Ciocalteau assay showed that the accumulation of PAs was markedly inhibited in 'Mopanshi', 'Eshi 1' and 'Youhou' leaves. These results indicate that DkLAR plays an important role in the accumulation of PAs in persimmon. The transient ihpRNA-induced gene silencing method developed in this study is a highly efficient and useful tool for functional analysis of persimmon genes involved in PA biosynthesis.
Rongli Mo,Na Zhang,Sichao Yang,Qinglin Zhang,Zhengrong Luo 한국원예학회 2016 원예과학기술지 Vol.34 No.2
A transient ihpRNA-induced gene silencing system based on Agrobacterium-mediated injection infiltration has been established to evaluate candidate genes involved in proanthocyanidin (PAs) biosynthesis in persimmon (Diospyros kaki Thunb.). We chose DkPDS (phytoene desaturase) as a gene-silencing target to evaluate the newly developed transient gene silencing system. Our qRT-PCR analysis indicated that two ihpRNA constructs (pHG-PDS5’ and pHG-PDS3’) targeted DkPDS, which also led to significantly reduce expression of DkPDS in ‘Mopanshi’ persimmon leaves. To further confirm the reliability of the system, we successfully utilized it for DkLAR (leucoanthocyanidin reductase) gene silencing. The expression levels of DkLAR in ‘Mopanshi’ and ‘Eshi 1’ leaves were ca. 6-fold and ca. 5-fold lower than those in leaves harboring empty vector (pHG-GFP), respectively. DMACA (4-dimethylaminocinnamaldehyde) staining and the Folin-Ciocalteau assay showed that the accumulation of PAs was markedly inhibited in ‘Mopanshi’, ‘Eshi 1’ and ‘Youhou’ leaves. These results indicate that DkLAR plays an important role in the accumulation of PAs in persimmon. The transient ihpRNA-induced gene silencing method developed in this study is a highly efficient and useful tool for functional analysis of persimmon genes involved in PA biosynthesis.
Peng Chen,Lei Yan,Shuang Zhang,Zhengrong Wu,Suyue Li,Xiaojuan Yan,Ningbo Wang,Ning Liang,Hong Yu Li 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.1
A stirring bioreactor packed with a carbon fiber textiles (FT) biofilm formed by Bacillus subtilis was used to produce vanillin from ferulic acid. Biofilm formation was characterized by scanning electron microscopy. The interactive effects of three variables on vanillin molar yield (M) and conversion efficiency of ferulic acid (E) were evaluated by response surface methodology (RSM) with a Box-Behnken design (BBD). The optimal conversion conditions with a maximum overall desirability D of 0.983 were obtained by a desirability function. Considering the actual operation, the confirmation tests were performed using the slightly modified optimal conditions (initial ferulic acid concentration 1.55 g/ L, temperature 35oC, stirring speed 220 rpm). The results showed that M and E were 57.42 and 93.53%, respectively. This was only 1.03% and 1.87%, respectively, different from the predicted values, confirming the validity of the predicted models. These revealed that the stirred packed reactor could be successfully used in vanillin bioconversion from ferulic acid.
Shougang Duan,Yufeng Yang,Yong Dong,Yu Wang,Beibei Jiang,Chuanqiang Li,Zhengrong Zhang 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.6
Ultra-fine grain AlCrFe2Ni2Wx(x = 0.1, 0.2, 0.3, 0.4) alloys were designed and prepared by vacuum arc melting, and correspondingmicrostructural evolution and mechanical properties were investigated. All of the alloys show a mixed structurewith FCC + BCC (B2) + TCP phases. The addition of W element has a significant effect on the formation mechanism of themorphology, which promotes the transition from FCC phase to μ phase and inhibits the spinodal decomposition of BCCphase. With the increase of W content, more μ phase precipitates from the FCC phase and grain boundaries. After the dissolutionof W element into matrix, the formed oversaturated solid solution and precipitated μ phase have the effect of solidsolution strengthening and second phase strengthening, respectively. The yield strength and Vickers hardness increasesfrom 765 to 1319.6 MPa and HV 332.2 to HV 461.8, respectively. The addition of W element enhances the strength of thealloys but reduced ductility. The AlCrFe2Ni2W0.1alloy exhibits outstanding comprehensive mechanical properties, with itsfracture strength reaching 2726.5 MPa and a considerable compressive strain of 43.3%, which implies promising potentialengineering application.
Effect of Li Content on the Surface Film Formed on the Binary Mg–Li Alloys in NaCl Solution
Chuanqiang Li,Dahui Liang,Yejia Lin,Yong Dong,Binqing Shi,Changjian Yan,Zhengrong Zhang 대한금속·재료학회 2024 METALS AND MATERIALS International Vol.30 No.1
The surface film formed on the Mg–Li alloys with different Li content in 0.1 M NaCl solution were investigated via electrochemicaltesting, morphologies observation and chemical composition analysis in this work. The results revealed that thesurface film of Mg–14Li alloy (L14: body centred cubic (BCC)) possessed higher electrical resistivity and remained a highertolerating over-potential (0.1 V vs. open circuit potential) than those of Mg–4Li (L4: hexagonal closed-packed (HCP)) andMg–7.5Li (L7: HCP+BCC), resulting in a better corrosion performance. After 24 h immersion in NaCl solution, the wholesurface film of L14 remained undamaged and displayed weave-like and dense characteristic, while both L4 and L7 present aseverely damaged film. The cross-sectional details illustrated typical two layers of surface film formed on L14 with the outerlayer of ~ 1.5 μm and inner layer of ~ 2.5 μm in thickness, whilst only one layer of film with less than 2.5 μm in thicknesswas observed on L4 and L7. At last, combining analysis of X-ray diffraction, energy dispersive X-ray spectrometry, X-rayphotoelectron spectroscopy and transmission electron microscope confirmed the composition of outer layer of surface filmon the L14 contains much more Mg(OH)2 and Li-containing compounds, and the porous Mg(OH)2 film can be sealed by theLi-containing compounds, which is very different the single Mg(OH)2 film on the traditional magnesium and L4/L7 alloys. As a result, the best protective function of the surface film on the L14 alloy contribute to its high corrosion resistance.