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      • KCI등재

        Sensorless Control of Double-Sided Linear Switched Reluctance Machines with Eccentricities

        Qianlong Wang,Zhengfei Wu,Wei Jiang 전력전자학회 2019 JOURNAL OF POWER ELECTRONICS Vol.19 No.5

        The Double-sided Linear Switched Reluctance Machine (DLSRM) suffers from complex eccentricities in practical operations. A novel sensorless control method for a DLSRM with eccentricities is developed in this paper. The influences of eccentricities on the machine inductance characteristics and the estimated positions in sensorless control systems are discussed. A new position index, which is independent of eccentricities, is proposed according to an analysis of a DLSRM equivalent magnetic circuit. On the basis of this position index, the starting and low-velocity operation of eccentric DLSRMs are achieved. Experimental results obtained in the laboratory validate the proposed method.

      • SCIESCOPUSKCI등재

        Sensorless Control of Double-Sided Linear Switched Reluctance Machines with Eccentricities

        Wang, Qianlong,Wu, Zhengfei,Jiang, Wei The Korean Institute of Power Electronics 2019 JOURNAL OF POWER ELECTRONICS Vol.19 No.5

        The Double-sided Linear Switched Reluctance Machine (DLSRM) suffers from complex eccentricities in practical operations. A novel sensorless control method for a DLSRM with eccentricities is developed in this paper. The influences of eccentricities on the machine inductance characteristics and the estimated positions in sensorless control systems are discussed. A new position index, which is independent of eccentricities, is proposed according to an analysis of a DLSRM equivalent magnetic circuit. On the basis of this position index, the starting and low-velocity operation of eccentric DLSRMs are achieved. Experimental results obtained in the laboratory validate the proposed method.

      • KCI등재

        Identification of putative ingestion‑related olfactory receptor genes in the Chinese mitten crab (Eriocheir japonica sinensis)

        Chenchen Shen,Dan Tang,Yiping Zhang,Lv Wu,Yaqi Luo,Boping Tang,Zhengfei Wang 한국유전학회 2021 Genes & Genomics Vol.43 No.5

        Background Olfaction plays a central role in mating, spawning, obtaining food and escaping predators, which is essential for survival and reproduction of animals. The nature of the olfactory perception in crabs, which is a major group of crustaceans, has remained elusive. Objective This project aims to explore the molecular mechanism of olfaction in crabs and further improve our understanding of olfactory perception in crustaceans. Methods The olfactory receptors and ingestion-related gene expression in Eriocheir japonica sinensis were studied by transcriptomic techniques. The de novo assembly, annotation and functional evaluation were performed with bioinformatics tools. Results A series of chemosensory receptors associated with olfaction were identifed including 33 EsIRs, 24 EsIGluRs, 58 EsVIGluRs, 1 EsOR and 1 EsGC-D. We found IRs were key odorant receptors demonstrating a specifc species evolutionary trend in crustaceans. Furthermore, we identifed ORs in E. j. sinensis and Litopenaeus vannamei. The incomplete EsOR and LvOR1 structures implied that ORs exist in crustaceans, and may have been degenerated or even lost in the olfactory evolutionary process. In addition, comparative transcriptome analysises demonstrated two possible olfactory transduction pathways of E. j. sinensis: the cGMP-mediated olfactory pathway related to vegetable odor molecules and the cAMP-mediated olfactory pathway related to meat odor molecules. The above results were consistent with its omnivorous ingestion of E. j. sinensis. Conclusions Our study revealed the unique olfactory molecular mechanism of omnivorous crabs and provided valuable information for further functional research on the chemoreception mechanisms in crustaceans.

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        Mitochondrial OXPHOS genes provides insights into genetics basis of hypoxia adaptation in anchialine cave shrimps

        Huayun Guo,Hao Yang,Yitao Tao,Dan Tang,Qiong Wu,Zhengfei Wang,Bo-Ping Tang 한국유전학회 2018 Genes & Genomics Vol.40 No.11

        Cave shrimps from the genera Typhlatya, Stygiocaris and Typhlopatsa (TST complex) comprises twenty cave-adapted taxa, which mainly occur in the anchialine environment. Anchialine habitats may undergo drastic environmental fluctuations, including spatial and temporal changes in salinity, temperature, and dissolved oxygen content. Previous studies of crustaceans from anchialine caves suggest that they have possessed morphological, behavioral, and physiological adaptations to cope with the extreme conditions, similar to other cave-dwelling crustaceans. However, the genetic basis has not been thoroughly explored in crustaceans from anchialine habitats, which can experience hypoxic regimes. To test whether the TST shrimpcomplex hypoxia adaptations matched adaptive evolution of mitochondrial OXPHOS genes. The 13 OXPHOS genes from mitochondrial genomes of 98 shrimps and 1 outgroup were examined. For each of these genes was investigated and compared to orthologous sequences using both gene (i.e. branch-site and Datamonkey) and protein (i.e. TreeSAAP) level approaches. Positive selection was detected in 11 of the 13 candidate genes, and the radical amino acid changes sites scattered throughout the entire TST complex phylogeny. Additionally, a series of parallel/convergent amino acid substitutions were identified in mitochondrial OXPHOS genes of TST complex shrimps, which reflect functional convergence or similar genetic mechanisms of cave adaptation. The extensive occurrence of positive selection is suggestive of their essential role in adaptation to hypoxic anchialine environment, and further implying that TST complex shrimps might have acquired a finely capacity for energy metabolism. These results provided some new insights into the genetic basis of anchialine hypoxia adaptation.

      • KCI등재

        The complete mitochondrial genomes of Tarsiger cyanurus and Phoenicurus auroreus: a phylogenetic analysis of Passeriformes

        Huabin Zhang,Yuze Bai,Xuejia Shi,Linxia Sun,Zhengfei Wang,Xiaobing Wu 한국유전학회 2018 Genes & Genomics Vol.40 No.2

        Passeriformes is the largest group within aves and the phylogenetic relationships between Passeriformes have caused major disagreement in ornithology. Particularly, the phylogenetic relationships between muscicapoidea and sylvioidea are complex, and their taxonomic boundaries have not been clearly defined. Our aim was to study the status of two bird species: Tarsiger cyanurus and Phoenicurus auroreus. Furthermore, we analyzed the phylogenetic relationships of Passeriformes. Complete mitochondrial DNA (mtDNA) sequences of both species were determined and the lengths were 16,803 (T. cyanurus) and 16,772 bp (P. auroreus), respectively. Thirteen protein-coding genes, 22 tRNA genes, two rRNA genes, and one control region were identified in these mtDNAs. The contents of A and T at the base compositions was significantly higher than the content of G and C, and this AT skew was positive, while the GC skew was negative. The monophyly of Passeriformes is divided into four major clades: Corvoidea, Sylvioidea, Passeroidea, and Musicicapoidea. Paridae should be separated from the superfamily Sylvioidea and placed within the superfamily Muscicapoidea. The family Muscicapidae and Corvida were paraphyly, while Carduelis and Emberiza were grouped as a sister taxon. The relationships between some species of the order passeriformes may remain difficult to resolve despite an effort to collect additional characters for phylogenetic analysis. Current research of avian phylogeny should focus on adding characters and taxa and use both effectively to obtain a better resolution for deeper and shallow nodes.

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