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- 저자 : Zhengchao Wang (검색결과 3 건)
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Zhengchao Wang,Yanqing Wu,Liyun Chen,Qianping Luo,Jisen Zhang,Jiajie Chen,Zimiao Luo,Xiaohong Huang,Yong Cheng,Zhenghong Zhang 생화학분자생물학회 2012 Experimental and molecular medicine Vol.44 No.10
Echinomycin is a small-molecule inhibitor of hypoxia- inducible factor-1 DNA-binding activity, which plays a crucial role in ovarian ovulation in mammalians. The present study was designed to test the hypothesis that hypoxia-inducible factor (HIF)-1α-mediated endothelin (ET)-2 expressions contributed to ovarian ovulation in response to human chorionic gonadotropin (hCG) during gonadotropin-induced superuvulation. By real-time RT-PCR analysis, ET-2 mRNA level was found to significantly decrease in the ovaries after chinomycin treatment, while HIF-1α mRNA and protein expression was not obviously changed. Further analysis also showed that these changes of ET-2 mRNA were consistent with HIF-1 activity in the ovaires, which is similar with HIF-1α and ET-2 expression in the granulosa cells with gonadotropin and echinomycin treatments. The results of HIF-1α and ET-2 expression in the granulosa cells transfected with cis-element oligodeoxynucleotide (dsODN) under gonadotropin treatment further indicated HIF-1α directly mediated the transcriptional activation of ET-2 during gonadotropin- induced superuvulation. Taken together, these results demonstrated that HIF-1α-mediated ET-2 transcriptional activation is one of the important mechanisms regulating gonadotropin-induced mammalian ovulatory precess in vivo. Echinomycin is a small-molecule inhibitor of hypoxia- inducible factor-1 DNA-binding activity, which plays a crucial role in ovarian ovulation in mammalians. The present study was designed to test the hypothesis that hypoxia-inducible factor (HIF)-1α-mediated endothelin (ET)-2 expressions contributed to ovarian ovulation in response to human chorionic gonadotropin (hCG) during gonadotropin-induced superuvulation. By real-time RT-PCR analysis, ET-2 mRNA level was found to significantly decrease in the ovaries after chinomycin treatment, while HIF-1α mRNA and protein expression was not obviously changed. Further analysis also showed that these changes of ET-2 mRNA were consistent with HIF-1 activity in the ovaires, which is similar with HIF-1α and ET-2 expression in the granulosa cells with gonadotropin and echinomycin treatments. The results of HIF-1α and ET-2 expression in the granulosa cells transfected with cis-element oligodeoxynucleotide (dsODN) under gonadotropin treatment further indicated HIF-1α directly mediated the transcriptional activation of ET-2 during gonadotropin- induced superuvulation. Taken together, these results demonstrated that HIF-1α-mediated ET-2 transcriptional activation is one of the important mechanisms regulating gonadotropin-induced mammalian ovulatory precess in vivo.
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Jie Zhou,Zhengchao Tan,Linwei CaoLingze Meng,Yuexing Wang 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.5
In the present research, the effects of normal mean stress acting on fatigue evolution are considered well in critical plane multiaxial models, whereas shear mean stress effects have rarely attracted attention. These models may result in nonconservative predictions under asymmetric loadings with substantial shear mean stress. Therefore, an improved critical energy model is introduced to solve multiaxial fatigue issues on the basis of the Fatemi-Socie model and the Walker model, which consider normal mean effect, shear mean stress effect, and additional hardening effect. Moreover, experimental data from GH4169, wrought Ti-6Al-4V, and TC4 are employed to validate the proposed model. Results show that the proposed model can reasonably predict fatigue life under various loadings, particularly for asymmetric loadings.
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Zhiyun Zhang,Jian Wang,Weiping Wan,Zhengchao Shen,Aixue Zuo,Rong Chen,Qinyi Wu,Enli Cai,Feng Huang,Rongping Zhang,Xinan Shi 한국통합생물학회 2023 Animal cells and systems Vol.27 No.1
Hepatocellular carcinoma (HCC) is a frequently occurring malignant gastrointestinal cancer. The 5-year survival rate of HCC is still below 8%, and thus, identifying more effective therapeutic methodsis needed. Here, we evaluated the effects of Stigmast-4-en-6β-ol-3-one (S463) on the viability andcolony formation of liver cancer cells. S463 treatment decreased the viability and inducedapoptosis and ferroptosis in liver cancer cells, and also increased cellular malondialdehyde(MDA) and lipid peroxidation levels. In S463 treated cells, the expression level of Bax wasincreased, and the expression level of GPX4 was reduced, and the cleavage of PARP wasimproved. We also found that S463 treatment downregulated E2F1 and upregulated p53 atboth the mRNA and protein levels. Importantly, rescue experiments revealed thatoverexpression of E2F1 partially restored S463-induced Bax and p53 upregulation and GPX4downregulation and counteracted the S463-induced decrease in cell viability and colonyformation and the S463-induced increase in MDA and lipid peroxidation levels. Our findingssuggest that S463 significantly inhibits viability and colony formation and induces apoptosisand ferroptosis in liver cancer cells via E2F1.
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