Long Noncoding RNA MALAT1 Regulates Hepatocellular Carcinoma Growth Under Hypoxia via Sponging MicroRNA-200a

Zheng-Bin Zhao,Fei Chen,Xiao-Fang Bai 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.8

Purpose: Hepatocellular carcinoma (HCC) is a common cancer worldwide. Metastasis-associated lung adenocarcinoma transcript1 (MALAT1), a long noncoding RNA (lncRNA), has been reported to be aberrantly expressed in hypoxic cancer cells. MALAT1 playsa significant role in many malignancies, including HCC. The aim of this study was to explore the role of MALAT1 in hypoxic HCCcells and its underlying regulatory mechanism. Materials and Methods: Quantitative reverse transcription PCR (qRT-PCR) assay was performed to detect the mRNA levels ofMALAT1 and microRNA-200a (miR-200a) in HCC cells. Cell invasion and migration ability were evaluated by Transwell assay. Starbasev2.0 and luciferase reporter assay were employed to identify the association between MALAT1 and miR-200a. Cell proliferationand apoptosis were measured by MTT assay and flow cytometry, respectively. Results: MALAT1 levels were significantly upregulated in HCC cells under hypoxia. Hypoxia promoted proliferation, migration,and invasion, and blocked apoptosis in Hep3B cells, which were weakened by knockdown of MALAT1. Starbase v2.0 showed thatMALAT1 and miR-200a have a complementarity region, and luciferase reporter assay verified that MALAT1 interacted with miR-200a in Hep3B cells. Moreover, MALAT1 negatively regulated the expression of miR-200a. miR-200a levels were dramatically downregulatedin HCC cells under hypoxia. Upregulation of miR-200a inhibited proliferation, migration, and invasion, and inducedapoptosis in Hep3B cells under hypoxia. Interestingly, downregulation of miR-200a partially reversed the tumor-suppressive effectof knockdown of MALAT1 on Hep3B cells in hypoxic condition. Conclusion: LncRNA MALAT1 was involved in proliferation, migration, invasion, and apoptosis by interacting with miR-200a inhypoxic Hep3B cells, revealing a new mechanism of MALAT1 involved in hypoxic HCC progression.

Vesicular Stomatitis Virus G Glycoprotein and ATRA Enhanced Bystander Killing of Chemoresistant Leukemic Cells by Herpes Simplex Virus Thymidine Kinase/Ganciclovir

( Bin Yin ),( Chen Xi Hu ),( Zheng Chen ),( Wen Jun Zhao ),( Li Rong Wei ),( Yan Wen Zheng ),( Chao He ),( Yan Zeng ) 한국응용약물학회 2014 Biomolecules & Therapeutics(구 응용약물학회지) Vol.22 No.2

Vesicular Stomatitis Virus G Glycoprotein and ATRA EnhancedBystander Killing of Chemoresistant Leukemic Cells by HerpesSimplex Virus Thymidine Kinase/Ganciclovir

Characteristics of Mammary Paget's Disease in China: a National-wide Multicenter Retrospective Study During 1999-2008

Zheng, Shan,Song, Qing-Kun,Zhao, Lin,Huang, Rong,Sun, Li,Li, Jing,Fan, Jin-Hu,Zhang, Bao-Ning,Yang, Hong-Jian,Xu, Feng,Zhang, Bin,Qiao, You-Lin Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

The aim of this study was to detail characteristics of mammary Paget's disease (PD) representing the whole population in China. A total of 4211 female breast cancer inpatients at seven tertiary hospitals from seven representative geographical regions of China were collected randomly during 1999 to 2008. Data for demography, risk factors, diagnostic imaging test, physical examination and pathologic characters were surveyed and biomarker status was tested by immunohistochemistry. The differences of demography and risk factors between PD with breast cancer and other lesions were compared using Chi-square test or t-test, with attention to physical examination and pathological characters. The percentage of PD was 1.6% (68/4211) in all breast cancers. The mean age at diagnosis was 48.1, and 63.2% (43/68) patients were premenopausal. There is no difference in demography and risk factors between PD with breast cancer and other breast cancer (P > 0.05). The main pattern of PD in physical exam and pathologic pattern were patients presenting with a palpable mass in breast (65/68, 95.6%) and PD with underlying invasive cancer (82.4%, 56/68) respectively. The rate of multifocal disease was 7.4% (5/68). PD with invasive breast cancer showed larger tumor size, more multifocal disease, lower ER and PR expression and higher HER2 overexpression than those in other invasive breast cancer (P < 0.05). These results suggested that PD in China is a concomitant disease of breast cancer, and that PD with underlying invasive cancer has more multiple foci and more aggressive behavior compared with other breast invasive cancer. We address the urgent needs for establishing diagnostic and therapeutic guidelines for mammary PD in China.

The Efficacy of Saccharomyces boulardii CNCM I-745 in Addition to Standard Helicobacter pylori Eradication Treatment in Children

Zhang, Bin,Xu, Ya-Zheng,Deng, Zhao-Hui,Chu, Bo,Jiang, Li-Rong,Vandenplas, Yvan The Korean Society of Pediatric Gastroenterology 2015 Pediatric gastroenterology, hepatology & nutrition Vol.18 No.1

Purpose: This study aims to investigate Saccharomyces boulardii CNCM I-745 during Helicobacter pylori eradication in children. Methods: One hundred ninety-four H. pylori positive children were randomized in two groups. Therapy (omeprazole+ clarithromycin+amoxicillin or omeprazole+clarithromycin+metronidazole in case of penicillin allergy) was given to both groups during two weeks. In the treatment group (n: 102) S. boulardii was added to the triple therapy, while the control group (n: 92) only received triple therapy. The incidence, onset, duration and severity of diarrhea and compliance to the eradication treatment were compared. A $^{13}C$ urea breath test was done 4 weeks after the end of eradication therapy in two groups of 21 patients aged 12 years and older to test the H. pylori eradication rate. Results: In the treatment group, diarrhea occurred in 12 cases (11.76%), starting after $6.25{\pm}1.24days$, lasting $3.17{\pm}1.08days$, and compliance to eradication treatment was 100%. In the control group, diarrhea occurred in 26 cases (28.26%), starting after $4.05{\pm}1.11days$, lasting $4.02{\pm}0.87days$, and in six cases eradication treatment was stopped prematurely (p<0.05). The $^{13}C$ urea breath test showed successful H. pylori eradication in 71.4% of the patients in the treatment and in 61.9 % in the control group (not significant). Conclusion: S. boulardii has a beneficial effect on the prevention and treatment of diarrhea during H. pylori eradication in children. Although S. boulardii did only slightly increase H. pylori eradication rate, compliance to eradication treatment was improved.

Effects of Reynolds number on the flow field in a low-pressure turbine with incoming wakes

Xu Zhao,Yunfei Wang,Xiaozhong Ma,Yaowen Zhang,Long Yue,Bin Zheng 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.11

In order to further understand the effects of the Reynolds number on the flow field within a low-pressure turbine with incoming wakes, the transition SST turbulence model was employed for numerical simulation under four Reynolds number conditions ranging from 0.6×10 5 to 3.0×10 5 . The numerical results showed that the boundary layer separation at the rear part of the suction surface was prone to occur in the condition of low Reynolds number (Re 2th = 0.6×10 5 ), and the separation bubble was semi-open and semi-closed. The separation bubble impedes the development of the passage vortex and wall vortex towards mid-span region. When the Reynolds number exceeds 1.0×10 5 , boundary layer separation does not occur. The influence of Reynolds number on the total pressure loss coefficient is primarily evident on the suction surface side, and the growth rate of total pressure loss coefficient first increases and then decreases along the streamwise direction. As the Reynolds number increases from 0.6×10 5 to 3.0×10 5 , the peak point location of total pressure loss decreases by approximately 12 %. The influence of Reynolds number on corner vortex and wall vortex is relatively more sensitive compared to that of passage vortex.

The Effect of New Ecological Paradigm on Pro-Environmental Behaviors: Mediating Role of Environmental Attitudes

Kai-bin Zhao,Zheng Jin,Timothy Tamunang Tamutana,Yi-Ming Shi,Marina Kogay,Hanna Yeshinegus Adamseged 한국공공가치학회 2021 공공가치연구 Vol.1 No.1

Acquiring a better understanding of what drives pro-environmental behaviors is important for human. This study explores the underlying mechanism between New Ecological Paradigm and pro-environmental behaviors. Based on cognitive dissonance theory and identity theory, a questionnaire, including New Ecological Paradigm scale, pro-environmental attitude scale and pro-environmental behaviors scales, was used to investigate 1352 university students. The results indicated that: (1) There were significant correlations among New Ecological Paradigm, pro-environmental attitude and pro-environmental behaviors; (2) pro-environmental attitude played mediating role between New Ecological Paradigm and pro-environmental behaviors. In summary, this study uncovered the mechanism underlying the relationship between New Ecological Paradigm and pro-environmental behaviors, which contributes to the understanding of the complex mechanism that influence people s pro-environmental behaviors.

Resonant Frequency Estimation of Reradiation Interference at MF from Power Transmission Lines Based on Generalized Resonance Theory

Bo, Tang,Bin, Chen,Zhibin, Zhao,Zheng, Xiao,Shuang, Wang The Korean Institute of Electrical Engineers 2015 Journal of Electrical Engineering & Technology Vol.10 No.3

The resonant mechanism of reradiation interference (RRI) over 1.7MHz from power transmission lines cannot be obtained from IEEE standards, which are based on researches of field intensity. Hence, the resonance is ignored in National Standards of protecting distance between UHV power lines and radio stations in China, which would result in an excessive redundancy of protecting distance. Therefore, based on the generalized resonance theory, we proposed the idea of applying model-based parameter estimation (MBPE) to estimate the generalized resonance frequency of electrically large scattering objects. We also deduced equation expressions of the generalized resonance frequency and its quality factor Q in a lossy open electromagnetic system, i.e. an antenna-transmission line system in this paper. Taking the frequency band studied by IEEE and the frequency band over 1.7 MHz as object, we established three models of the RRI from transmission lines, namely the simplified line model, the tower line model considering cross arms and the line-surface mixed model. With the models, we calculated the scattering field of sampling points with equal intervals using method of moments, and then inferred expressions of Padé rational function. After calculating the zero-pole points of the Padé rational function, we eventually got the estimation of the RRI’s generalized resonant frequency. Our case studies indicate that the proposed estimation method is effective for predicting the generalized resonant frequency of RRI in medium frequency (MF, 0.3~3 MHz) band over 1.7 MHz, which expands the frequency band studied by IEEE.

Resonant Frequency Estimation of Reradiation Interference at MF from Power Transmission Lines Based on Generalized Resonance Theory

Tang Bo,Chen Bin,Zhao Zhibin,Xiao Zheng,Wang Shuang 대한전기학회 2015 Journal of Electrical Engineering & Technology Vol.10 No.3

The resonant mechanism of reradiation interference (RRI) over 1.7MHz from power transmission lines cannot be obtained from IEEE standards, which are based on researches of field intensity. Hence, the resonance is ignored in National Standards of protecting distance between UHV power lines and radio stations in China, which would result in an excessive redundancy of protecting distance. Therefore, based on the generalized resonance theory, we proposed the idea of applying model-based parameter estimation (MBPE) to estimate the generalized resonance frequency of electrically large scattering objects. We also deduced equation expressions of the generalized resonance frequency and its quality factor Q in a lossy open electromagnetic system, i.e. an antennatransmission line system in this paper. Taking the frequency band studied by IEEE and the frequency band over 1.7 MHz as object, we established three models of the RRI from transmission lines, namely the simplified line model, the tower line model considering cross arms and the line-surface mixed model. With the models, we calculated the scattering field of sampling points with equal intervals using method of moments, and then inferred expressions of Pade rational function. After calculating the zero-pole points of the Pade rational function, we eventually got the estimation of the RRI’s generalized resonant frequency. Our case studies indicate that the proposed estimation method is effective for predicting the generalized resonant frequency of RRI in medium frequency (MF, 0.3~3 MHz) band over 1.7 MHz, which expands the frequency band studied by IEEE.

Metagenomic and Proteomic Analyses of a Mangrove Microbial Community Following Green Macroalgae Enteromorpha prolifera Degradation

( Yijing Wu ),( Chao Zhao ),( Zheng Xiao ),( Hetong Lin ),( Lingwei Ruan ),( Bin Liu ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.12

A mangrove microbial community was analyzed at the gene and protein levels using metagenomic and proteomic methods with the green macroalgae Enteromorpha prolifera as the substrate. Total DNA was sequenced on the Illumina HiSeq 2000 PE-100 platform. Twodimensional gel electrophoresis in combination with liquid chromatography tandem mass spectrometry was used for proteomic analysis. The metagenomic data revealed that the orders Pseudomonadales, Rhizobiales, and Sphingomonadales were the most prevalent in the mangrove microbial community. By monitoring changes at the functional level, proteomic analyses detected ATP synthase and transporter proteins, which were expressed mainly by members of the phyla Proteobacteria and Bacteroidetes. Members of the phylum Proteobacteria expressed a high number of sugar transporters and demonstrated specialized and efficient digestion of various glycans. A few glycoside hydrolases were detected in members of the phylum Firmicutes, which appeared to be the main cellulose-degrading bacteria. This is the first report of multiple “omics” analysis of E. prolifera degradation. These results support the fact that key enzymes of glycoside hydrolase family were expressed in large quantities, indicating the high metabolic activity of the community.

Patent Ductus Arteriosus and Pulmonary Valve Stenosis in A Patient with 18p Deletion Syndrome

Chun-Hong Xie,Jian-Bin Yang,Fang-Qi Gong,Zheng-Yan Zhao 연세대학교의과대학 2008 Yonsei medical journal Vol.49 No.3

We report on a patient with a partial deletion on the short arm of chromosome 18 (del 18p), who presented with dysmorphic features and delayed developmental milestones as well as with a patent ductus arteriosus (PDA) and pulmonary valve stenosis (PS). Several forms of congenital heart disease (CHD) are found in about 10% of patients with del (18p), but coexisting PDA and PS have not been reported. Del (18p) must be considered in patients with characteristic phenotypic abnormalities and congenital heart disease, including a combination of PDA and PS.