Conversion of incinerator bottom ash into mesoporous silica materials

Zhen-Shu Liu,Chun-Yi Huang,Sheng-Hua Chen 대한환경공학회 2015 대한환경공학회 학술발표논문집 Vol.2015 No.10

Experimental Research and Numerical Simulation of a Large-span Isolated Structure Considering Multi-dimensional Input Effects

Zhen-yuan Gu,Shu-guang Wang,Weiqing Liu,Dong-sheng Du,Wei-zhi Xu 한국강구조학회 2017 International Journal of Steel Structures Vol.17 No.4

Based on the shaking table test of 1:20 scale structural models of the large-span isolated and non-isolated flat grid structures under the action of horizontal-bidirectional (2D) and three-dimensional (3D) rare earthquake, the dynamic characteristics, including natural vibration frequency, damping ratio, the acceleration and displacement responses are investigated. The results show that the base isolation could significantly prolong the structural vibration periods and increase the damping ratio. The capacity of energy dissipation for the base-isolated structure can also be improved. The horizontal acceleration amplification factors (AAFs) of the structure can be reduced greatly due to the base isolation, which effectively reduces the vertical vibration of the large-span grid. Under the 3D seismic input, the horizontal AAF of each layer is greater than that under the 2D one, which illustrates that the vertical seismic input increases the horizontal dynamic response of the structure. Furthermore, the finite element models of the isolated and non-isolated structures were simulated. The experimental results are in good agreement with the numerical results. The parameter optimization of isolation bearings was conducted by using finite element software. The seismic response of each layer under different shear to weight ratios (μ) was analyzed. It can be found that the parameters of the isolation layer have an optimal range which makes the dynamic response of the structure reach a minimum value under the rare earthquake.

Fatty Acid Binding Protein 5 (FABP5) Promotes Aggressiveness of Gastric Cancer Through Modulation of Tumor Immunity

Shu Zhang,Mei-qing Qiu,Hui-jun Wang,Ya-fei Ju,Zhen Liu,Tao Wang,Shi-feng Kan,Zhen Yang,Ya-yun Cui,You-qiang Ke,Hong-min He,Li Sun 대한위암학회 2023 Journal of gastric cancer Vol.23 No.2

Purpose: Gastric cancer (GC) is the second most lethal cancer globally and is associated with poor prognosis. Fatty acid-binding proteins (FABPs) can regulate biological properties of carcinoma cells. FABP5 is overexpressed in many types of cancers; however, the role and mechanisms of action of FABP5 in GC remain unclear. In this study, we aimed to evaluate the clinical and biological functions of FABP5 in GC. Materials and Methods: We assessed FABP5 expression using immunohistochemical analysis in 79 patients with GC and evaluated its biological functions following in vitro and in vivo ectopic expression. FABP5 targets relevant to GC progression were determined using RNA sequencing (RNA-seq). Results: Elevated FABP5 expression was closely associated with poor outcomes, and ectopic expression of FABP5 promoted proliferation, invasion, migration, and carcinogenicity of GC cells, thus suggesting its potential tumor-promoting role in GC. Additionally, RNA-seq analysis indicated that FABP5 activates immune-related pathways, including cytokine-cytokine receptor interaction pathways, interleukin-17 signaling, and tumor necrosis factor signaling, suggesting an important rationale for the possible development of therapies that combine FABP5-targeted drugs with immunotherapeutics. Conclusions: These findings highlight the biological mechanisms and clinical implications of FABP5 in GC and suggest its potential as an adverse prognostic factor and/or therapeutic target.

Anti-tumor Efficacy of a Hepatocellular Carcinoma Vaccine Based on Dendritic Cells Combined with Tumor-derived Autophagosomes in Murine Models

Su, Shu,Zhou, Hao,Xue, Meng,Liu, Jing-Yu,Ding, Lei,Cao, Meng,Zhou, Zhen-Xian,Hu, Hong-Min,Wang, Li-Xin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

The majority of hepatocellular carcinoma (HCC) patients have a poor prognosis with current therapies, and new approaches are urgently needed. We have developed a novel therapeutic cancer vaccine platform based on tumor cell derived autophagosomes (DRibbles) for cancer immunotherapy. We here evaluated the effectiveness of DRibbles-pulsed dendritic cell (DC) immunization to induce anti-tumor immunity in BALB/c mouse HCC and humanized HCC mouse models generated by transplantation of human HCC cells (HepG2) into BALB/c-nu mice. DRibbles were enriched from H22 or BNL cells, BALB/c-derived HCC cell lines, by inducing autophagy and blocking protein degradation. DRibbles-pulsed DC immunization induced a specific T cell response against HCC and resulted in significant inhibition of tumor growth compared to mice treated with DCs alone. Antitumor efficacy of the DCs-DRibbles vaccine was also demonstrated in a humanized HCC mouse model. The results indicated that HCC/DRibbles-pulsed DCs immunotherapy might be useful for suppressing the growth of residual tumors after primary therapy of human HCC.

Anti-Angiogenic Activity of Gecko Aqueous Extracts and its Macromolecular Components in CAM and HUVE-12 Cells

Tang, Zhen,Huang, Shu-Qiong,Liu, Jian-Ting,Jiang, Gui-Xiang,Wang, Chun-Mei Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.5

Gecko is a kind of traditional Chinese medicine with remarkable antineoplastic activity. However, undefined mechanisms and ambiguity regarding active ingredients limit new drug development from gecko. This study was conducted to assess anti-angiogenic properties of the aqueous extracts of fresh gecko (AG) or macromolecular components separated from AG (M-AG). An enzyme-linked immunosorbent assay (ELISA) approach was applied to detect the vascular endothelial growth factor (VEGF) secretion of the tumor cells treated with AG or M-AG. The effect of AG or M-AG on vascular endothelial cell proliferation and migratory ability was analyzed by tetrazolium dye colorimetric method, transwell and wound-healing assays. Chick embryo chorioallantoic membrane (CAM) assays were used to ensure the anti-angiogenic activity of M-AG in vivo. The results showed that AG or M-AG inhibited the VEGF secretion of tumor cells, the relative inhibition rates of AG and M-AG being 27.2% and 53.2% respectively at a concentration of $20{\mu}L/mL$. AG and M-AG inhibited the vascular endothelial (VE) cell proliferation with IC50 values of $11.5{\pm}0.5{\mu}L/mL$ and $12.9{\pm}0.4{\mu}L/mL$ respectively. The VE cell migration potential was inhibited significantly (p<0.01) by the AG (${\geq}24{\mu}L/mL$) or M-AG (${\geq}12\mu}L/mL$) treatment. In vivo, neovascularization of CAM treated with M-AG was inhibited significantly (p<0.05) at a concentration of ${\geq}0.4{\mu}L/mL$. This study provided evidence that anti-angiogenesis is one of the anti-tumor mechanisms of AG and M-AG, with the latter as a promising active component.

RNAi-based Knockdown of Multidrug Resistance-associated Protein 1 is Sufficient to Reverse Multidrug Resistance of Human Lung Cells

Shao, Shu-Li,Cui, Ting-Ting,Zhao, Wei,Zhang, Wei-Wei,Xie, Zhen-Li,Wang, Chang-He,Jia, Hong-Shuang,Liu, Qian Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

Up-regulation of multidrug resistance-associated protein 1 (MRP1) is regarded as one of the main causes for multidrug resistance (MDR) of tumor cells, leading to failure of chemotherapy-based treatment for a multitude of cancers. However, whether silencing the overexpressed MRP1 is sufficient to reverse MDR has yet to be validated. This study demonstrated that RNAi-based knockdown of MRP1 reversed the increased efflux ability and MDR efficiently. Two different short haipin RNAs (shRNAs) targeting MRP1 were designed and inserted into pSilence-2.1-neo. The shRNA recombinant plasmids were transfected into cis-dichlorodiamineplatinum-resistant A549 lung (A549/DDP) cells, and then shRNA expressing cell clones were collected and maintained. Real time PCR and immunofluorescence staining for MRP1 revealed a high silent efficiency of these two shRNAs. Functionally, shRNA-expressing cells showed increased rhodamine 123 retention in A549/DDP cells, indicating reduced efflux ability of tumor cells in the absence of MRP1. Consistently, MRP1-silent cells exhibited decreased resistance to 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and DDP, suggesting reversal of MDR in these tumor cells. Specifically, MRP1 knockdown increased the DDP-induced apoptosis of A549/DDP cells by increased trapping of their cell cycling in the G2 stage. Taken together, this study demonstrated that RNAi-based silencing of MRP1 is sufficient to reverse MDR in tumor cells, shedding light on possible novel clinical treatment of cancers.

Study of Recellularized Human Acellular Arterial Matrix Repairs Porcine Biliary Segmental Defects

Wei Liu,Sheng-Ning Zhang,Zong-Qiang Hu,Shi-Ming Feng,Zhen-Hui Li,Shu-Feng Xiao,Hong-Shu Wang,Li Li 한국조직공학과 재생의학회 2019 조직공학과 재생의학 Vol.16 No.6

BACKGROUND: With the popularity of laparoscopic cholecystectomy, common bile duct injury has been reported more frequently. There is no perfect method for repairing porcine biliary segmental defects. METHODS: After the decellularization of human arterial blood vessels, the cells were cultured with GFP? (carry green fluorescent protein) porcine bile duct epithelial cells. The growth and proliferation of porcine bile duct epithelial cells on the human acellular arterial matrix (HAAM) were observed by hematoxylin–eosin (HE) staining, electron microscopy, and immunofluorescence. Then, the recellularized human acellular arterial matrix (RHAAM) was used to repair biliary segmental defects in the pig. The feasibility of it was detected by magnetic resonance cholangiopancreatography, liver function and blood routine changes, HE staining, immunofluorescence, real-time quantitative PCR (RT-qPCR), and western blot. RESULTS: After 4 weeks (w) of co-culture of HAAM and GFP? porcine bile duct epithelial cells, GFP? porcine bile duct epithelial cells grew stably, proliferated, and fused on HAAM. Bile was successfully drained into the duodenum without bile leakage or biliary obstruction. Immunofluorescence detection showed that GFP-positive bile duct cells could still be detected after GFP-containing bile duct cells were implanted into the acellular arterial matrix for 8 w. The implanted bile duct cells can successfully resist bile invasion and protect the acellular arterial matrix until the newborn bile duct is formed. CONCLUSION: The RHAAM can be used to repair biliary segmental defects in pigs, which provides a new idea for the clinical treatment of common bile duct injury.

Microstructure evolution and enhanced vacuum tribological performance of Ni-doped WS₂ composite coating

Xu, Shu-Sheng,Weng, Li-Jun,Liu, Yu-Zhen,Kang, Kyeong-Hee,Kim, Chang-Lae,Kim, Dae-Eun Elsevier Sequoia 2017 Surface & coatings technology Vol.325 No.-

<P><B>Abstract</B></P> <P>Ni-doped WS<SUB>2</SUB> composite coatings with various Ni contents were co-deposited using a radio frequency sputtering system on silicon wafer and AISI 440C stainless steel substrates. The microstructural characteristics of the WS<SUB>2</SUB>-Ni composite coatings and their tribological properties in vacuum were assessed. During introduction of Ni dopant in the WS<SUB>2</SUB>-Ni composite coating the sulfur/tungsten (S/W) ratio in the coating increased due to reduced preferential resputtering of sulfur atoms in the growing coating. The microstructure of the WS<SUB>2</SUB>-Ni composite coating varied from a fine columnar structure for Ni content equal to or less than 7.7at.% to a featureless structure as the Ni content increased further. The Ni dopant inhibited the growth of the coarse columnar WS<SUB>2</SUB> platelets which was accompanied by nanocrystallization and amorphization of the composite coating structure. WS<SUB>2</SUB>-Ni composite coatings with fine columnar structure exhibited relatively low hardness but showed a high tendency to form a lubricating transfer layer. It also demonstrated low brittleness and prolonged wear life in vacuum condition compared to coatings with dense featureless structure. The variation in tribological performance between the composite coatings resulted from the different wear mechanisms associated with their distinct microstructures.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Microstructure of WS<SUB>2</SUB> coating was modified by incorporating Ni as a dopant. </LI> <LI> The introduction of Ni increased the S/W ratio of the WS<SUB>2</SUB> composite coating. </LI> <LI> High Ni content in the WS<SUB>2</SUB> coating led to high brittleness and low wear resistance. </LI> <LI> WS<SUB>2</SUB>–5at.% Ni coating showed 5 times longer wear life than pure WS<SUB>2</SUB> film in vacuum. </LI> <LI> Superior tribological properties were attributed to transfer layer and high hardness. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Development of CMAQ for East Asia CO2 data assimilation under an EnKF framework: a first result

Huang, Zhekun,Peng, Zhen,Liu, Hongnian,Zhang, Meigen,Ma, Xiaoguang,Yang, Shu-Chih,Lee, Sang-Deok,Kim, Seung-Yeon Springer-Verlag 2014 Chinese science bulletin Vol.59 No.25

Evaluation of the Mutagenicity of Leachates Obtained from an Incinerator Fly Ash using a Salmonella Reverse Mutation Assay

Po-Wen Chen,Zhen-Shu Liu,Min-Jie Wun 대한환경공학회 2015 대한환경공학회 학술발표논문집 Vol.2015 No.10