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RISS 인기검색어
- 검색키워드
- 저자 : Zhaomin Wu (검색결과 4 건)
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Jie Wu,Xueqi Wang,Zhaomin Yao,Qingqing Wu,Wei Fang,Zegeng Li,Dianlei Wang 대한약학회 2019 Archives of Pharmacal Research Vol.42 No.11
In the present study, the roles of AITC in upregulatingthe MRP1 expression and its relationship with theactivation of the Notch1 signaling pathway were investigatedby combining the in vivo and in vitro experiments. AITCwas administered to the COPD model rats and normal ratsto explore the association between Notch1 and MRP1. Thehuman bronchial epithelial cells were treated with DAPT,the Notch1 signaling pathway inhibitor, to verify the effectof Notch1 on the expression of AITC-induced MRP1. Comparedwith the control group, the expressions of Notch1,Hes1 (the target gene of Notch1) and MRP1 in the lung tissueof the COPD model group were significantly inhibited. In contrast to the COPD model group, the expressions ofMRP1, Hes1 and Notch1 dramatically up-regulated followingthe treatment with Low/High doses of AITC. The expressionof MRP1 in the 16 HBE cells was down-regulated bythe inhibition of Notch in a DAPT concentration-dependentmanner. Additionally, the AITC-induced up-regulation ofthe MRP1 expression was markedly impaired following the inhibition of Notch1. The above results indicated that thepulmonary function and the expression of MRP1 in COPDrats could be improved by AITC, which was partly dependenton the Notch1 signaling pathway. Therefore, targetingthe Notch signaling pathway may present as an effectivetherapeutic strategy for COPD treatment.
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Yechuan Huang,Zhaomin Wu,Yanrong Wang,Feng Li 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.4
To investigate the effects of pressure and temperature on lipoxygenase (LOX) in meat, changes in LOX activities in minced pork and in crude enzyme extracts under different conditions of pressure (131.82- 468.18 MPa), temperature (23.18-56.82oC), and duration (6.59-23.41 min) were evaluated using response surface methodology. LOX activities in extracts exhibited a decrease with an increasing treatment temperature, pressure, or duration. Each of these factors alone had a significant (p<0.05) effect on LOX activities. Activation of LOX in most minced pork treated at less than 400 MPa was observed. Treatment pressure and temperature, and interactions between pressure and temperature and between duration and pressure, significantly (p<0.05) affected LOX activities. LOX showed higher stability in muscles than in extracts during combined pressure-heat treatments. The most important factor affecting LOX was treatment temperature for extracts and treatment pressure for muscles.
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Jiao, Ting,Lei, Zhaomin,Wu, Jianping,Li, Fei,Casper, David P.,Wang, Jianfu,Jiao, Jianxin Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.11
Objective: This project aimed to evaluate the effects of both different additives and filling methods on nutritive quality, fermentation profile, and in situ digestibility of whole plant corn silage. Methods: Whole plant corn forage harvested at 26.72% dry matter (DM) was chopped and treated with two filling methods, i) fill silos at one time (F1), ii) fill silos at three times (F3), packing samples into one/three silo capacity at the first day, another one/three capacity at the second day, then one/three at the third day, three replicates. For each replicate, samples were treated with three additives, i) control (CTRL, no additive), ii) Sila-Max (MAX, Ralco Nutrition Inc., Marshall, MN, USA), and iii) Sila-Mix (MIX, Ralco Nutrition Inc., USA). With three replicates of each secondary treatment, there were nine silos, 54 silos in total. Each silo had a packing density of 137.61 kg of DM/m3. All silos were weighed and stored in lab at ambient temperature. Results: After 60 d of ensiling, all items showed good silage fermentation under MAX filled one time or three times (p<0.01). Higher silage quality for all additives was obtained at filling one time than that filled three times (p<0.01). The highest DM and lowest DM loss rate (DMLR) occurred to MAX treatment at two filling methods (p<0.01); Digestibility of acid detergent fiber, neutral detergent fiber (NDF), and curde protein had the same results as silage quality (p<0.01). Yield of digestible DM and digestible NDF also showed higher value under MAX especially for filling one time (p<0.05). Conclusion: All corn silages showed good fermentation attributes (pH<4.0). The forage filled one time had higher silage quality than that filled three times (p<0.01). MAX with homofermentative lactic acid bacteria enhanced the lactic acid fermentation, silage quality and nutrient digestibility, and so improved the digestible nutrient yield.
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Combined Effects of High-pressure and Thermal Treatments on Lipid Oxidation and Enzymes in Pork
Yechuan Huang,Yanrong Wang,Zhaomin Wu,Feng Li 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.1
The relationship between lipid oxidation and related enzymes in pork during combined pressure-heat treatments was investigated. Minced pork was treated under a pressure range of 0.1- 750 MPa and a temperature range of 30-60oC for 20 min. Thiobarbituric acid-reactive substances (TBARS) values and activities of acid lipase (AL), neutral lipase (NL), phospholipase (PL), and lipoxygenase (LOX) in pork were evaluated. NL and LOX were completely inactivated at 600 MPa-50oC and 450 MPa-60oC, and AL at 600MPa-55oC. PL had the greatest stability and was completely inactivated at 600 MPa-60oC and 750 MPa-50oC. PL activity was strongly related to lipid oxidation caused by high pressure, followed by AL and LOX. TBARS values at 600 and 750 MPa were strongly related to the inactivation rate and the ratio of PL during treatment. PL was the most important enzyme related to lipid oxidation induced by pressure.
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