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Yechuan Huang,Zhaomin Wu,Yanrong Wang,Feng Li 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.4
To investigate the effects of pressure and temperature on lipoxygenase (LOX) in meat, changes in LOX activities in minced pork and in crude enzyme extracts under different conditions of pressure (131.82- 468.18 MPa), temperature (23.18-56.82oC), and duration (6.59-23.41 min) were evaluated using response surface methodology. LOX activities in extracts exhibited a decrease with an increasing treatment temperature, pressure, or duration. Each of these factors alone had a significant (p<0.05) effect on LOX activities. Activation of LOX in most minced pork treated at less than 400 MPa was observed. Treatment pressure and temperature, and interactions between pressure and temperature and between duration and pressure, significantly (p<0.05) affected LOX activities. LOX showed higher stability in muscles than in extracts during combined pressure-heat treatments. The most important factor affecting LOX was treatment temperature for extracts and treatment pressure for muscles.
Combined Effects of High-pressure and Thermal Treatments on Lipid Oxidation and Enzymes in Pork
Yechuan Huang,Yanrong Wang,Zhaomin Wu,Feng Li 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.1
The relationship between lipid oxidation and related enzymes in pork during combined pressure-heat treatments was investigated. Minced pork was treated under a pressure range of 0.1- 750 MPa and a temperature range of 30-60oC for 20 min. Thiobarbituric acid-reactive substances (TBARS) values and activities of acid lipase (AL), neutral lipase (NL), phospholipase (PL), and lipoxygenase (LOX) in pork were evaluated. NL and LOX were completely inactivated at 600 MPa-50oC and 450 MPa-60oC, and AL at 600MPa-55oC. PL had the greatest stability and was completely inactivated at 600 MPa-60oC and 750 MPa-50oC. PL activity was strongly related to lipid oxidation caused by high pressure, followed by AL and LOX. TBARS values at 600 and 750 MPa were strongly related to the inactivation rate and the ratio of PL during treatment. PL was the most important enzyme related to lipid oxidation induced by pressure.