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        In vitro Development of Interspecies Somatic Cell Nuclear Transfer Embryos Derived from Murine Embryonic Fibroblasts and Bovine Oocytes

        Yun,,J.I.,Koo,,B.S.,Yun,,S.W.,Lee,,Chang-Kyu Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.11

        Interspecies somatic cell nuclear transfer (iSCNT) is a useful method to preserve endangered species and to study the reprogramming event of a nuclear donor cell by the oocyte. Although several studies of iSCNT using murine cells and bovine oocytes have been reported, the development of murine-bovine iSCNT embryos beyond the 8-cell stage has not been successful. In this paper, we examined the developmental potential of embryos reconstructed with a murine embryonic fibroblast as the nuclear donor and a bovine oocyte as the cytoplasm recipient. The reconstructed embryos were cultured in CZB (murine medium) or CR1aa (bovine medium). In addition, for the development of a murine-bovine iSCNT blastocyst, the antioxidant ${\beta}$-mercaptoethanol (${\beta}ME$) was supplemented to CR1aa medium. Furthermore, to verify the mouse genome activation in murine-bovine iSCNT embryos, RT-PCR analysis of murine Xist was performed. The development of the murine-bovine iSCNT embryos cultured in CR1aa was significantly higher than that in CZB (p<0.05). With respect to the effect of BME on the development of the murine-bovine iSCNT blastocyst, addition of BME produced a significant increase in blastocyst development (p<0.05). Karyotype analysis confirmed that the reconstructed embryos were derived from murine cells (40XX). The Xist gene was gradually increased from the 8-cell stage to the blastocyst stage. This is the first report of blastocyst development of iSCNT embryos derived from murine somatic cells and bovine oocytes. These results demonstrate that bovine cytoplasm can support the development of later stages of a preimplantation embryo from murine-bovine iSCNT.

      • KCI등재

        Changes of Tree Growth and Fruit Quality of "Yumi" Peach under Long-Term Soil Water Deficit

        Yun,,Seok,Kyu,Kim,,Sung,Jong,Nam,,Eun,Young,Kwon,,Jung,Hyun,Chung,,Kyeong,Ho,Choi,,In,Myung,Kim,,Ghiseok,Shin,,Hyunsuk Korean Society for Agricultural Machinery 2017 바이오시스템공학 Vol.42 No.4

        Purpose: This paper presents the effects of soil drought stress during the growing season and pre-harvest period on tree growth and fruit quality of "Yumi" peach, an early season cultivar. Methods: Soil drought stresses were treated with four levels of -30, -50, -60, and -70 kPa during long term (LT) and short term (ST). For LT treatments, soil water was controlled for nine weeks from May 1 to July 5, which was assumed as the full growing season. For ST treatments, soil water was controlled for four weeks from June 10 to July 5, which was assumed as the pre-harvest season. Tree growth and leaf photosynthesis were measured, and fruit characteristics such as fruit weight and diameter, soluble solid and tannin contents, and harvest date were investigated. Results: Soil water deficit treatments caused a significant reduction in tree growth, leaf photosynthesis, and fruit enlargement. LT water stress over -60 kPa during the full growing season caused significant reduction in tree growth, including shoot length, trunk girth, leaf photosynthesis, and fruit enlargement. ST water stress over -60 kPa during the pre-harvest period also induced significant reduction in leaf photosynthesis and fruit enlargement, while tree growth was not reduced. In terms of fruit quality, water stress over -50 kPa significantly reduced fruit weight, increased soluble solid and tannin contents, and delayed harvest time in both LT and ST treatments. Conclusions: As a result, it is assumed that LT water stress over -60 kPa can reduce both tree growth and fruit enlargement, whereas ST water stress over -50 kPa can reduce fruit enlargement without reducing tree growth. From an agricultural perspective, moderate water deficit like -50 kPa treatments could have positive effects, such increased fruit soluble solid contents along with minimal reduction in fruit size.

      • A Case of Steven-Johnson Syndrome after Live Bee Acupuncture (Bong-Chim)

        Yun-Jae,Seol,Tae-Kyu,Lee,Su-Jung,Park,Sang-Uk,Ko,Kyu-Hyung,Kim,An-Soo,Jang 순천향대학교 순천향의학연구소 2015 Journal of Soonchunhyang Medical Science Vol.21 No.2

        Bee stings can cause various skin reactions that usually resolve over several days; however, in some patients the venom acts as an allergen, causing a severe immunological response such as anaphylaxis. Bee stings can also induce chronic inflammation because the barbed stinging apparatus and venom sacs, along with the nerve plexus, can lodge in the skin. Chronic non-specific low back pain is the most common medical problem for which patients seek complementary and alternative medical treatment, including bee venom acupuncture. Bee venom acupuncture involves injecting diluted bee venom into acupoints and is used for arthritis, pain, and rheumatoid diseases. Here we report a 75-year-old man with acute urticaria and skin eruption with eosinophilia and interstitial pneumonia 6 weeks later after receiving 5 times bee venom acupuncture.

      • 베타3-아드레날린성 수용체 유전자 변이와 체지방 분포

        윤태승,김용득,김혜순,김미정,서영성,권중혁,최진수,김정국,하승우,김보완,원규장,이형우,손호상,이지현,윤현대,김원호,윤영길,이인규 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.2

        연구배경: 비만의 발생 원인은 크게 유전적인 요인과 환경적인 요인으로 나눌 수 있으며, 치근 비만과 관련된 유전자 연구가 활발히 진행되고 있다 지금 까지 알려진 비만 관련 유전자로는 Leptin, UCP, PPAR-r, β3-AR 유전자 등이 있으며, 이 중에서 β3-AR 유전자는 내장 지방에 주로 존재하면서 G 단백질과 결합하여, 아드레날린성 신호에 의해 c-AMP 증가로 지방 분해와 열 발생을 일으킨다. 이 유전자의 64번째 아미노산인 trpytophan이 arginine으로 치환된 변이에서 기초대사율 감소와 체중 증가가 있다는 보고가 있으나, 비만과 무관하다는 보고도 있어, 본 연구는 좀 더 많은 수를 대상으로 β3-AR 유전자 변이에 따른 비만과의 연관성을 확인 위해 시행되었다. 방법: 연구 대상자는 정상군 97명 비만군 101명으로 하여, β3-AR 유전자 변이에 따른 체질량지수, 허리둘레, 엉덩이둘레, 허리엉덩이둘레비, 복부 내장지방면적, 피하지방면적, 내장지방/피하지방면적비율을 비교하고, 총 콜레스테롤, 중성 지방, 고밀도 지단백, 저밀도 지단백 등을 비교하였다. 결과: β3-AR유전자의 다형성이 비만군에 있어 이형접합체변이 (Trp/Arg)와 동형접합체 변이 (Arg/Arg)비율이 43%, 5%로 정상인 36%, 1% 비해 높았으나 통계학적 유의성은 없었다. 복부 피하지방면적 비교에 있어 정상군(Trp/T게)에서는 213.9± 109.6 ㎠인 반면에 동형 접합체변이 (Trp/Arg)와 이형접합체변이 (Arg/Arg)군에서 각각, 244.0±127.7 ㎠, 323.9±189.8 ㎠로 통계학적 유의성이 있었으며, 그 외에 허리 둘레,엉덩이 둘레, 허리 엉덩이 둘레비, 내장지방면적, 내장지빈피하지방면적비 등은 유전자 변이군에서 전반적인 증가는 있었지만 통계학적 유의성은 없었다. 결론: 3-AR 유전자 변이와 비만과의 관련에 대한 본 연구에 의하면, β3-AR 유전자 변이에 따른 비만 연관성은 복부 피하지방면적 증가에 있어서 통계학적으로 유의하게 증가 소견 보였으며, 그 외 다른 비만 관련 인자들에 있어 통계학적 의의는 없으나 전반적인 증가 소견을 보여, 한국인에 있어 β3-AR 유전자 변이는 비만과 연관성이 있을 것이라는 결론을 얻을 수 있었다. Background: Reasons for obesity include environmental facotrs and, more largely so, genetic factors. There have been many studies on these genetic factors. So far, genes related to obesity such as Leptin, Uncoupling Protein(UCP), Peroxisome proliferator activated receptor-gamma (PPAR-γ), and Beta3-adrener-gic receptor(β3-AR) gene have been discovered. Among these, β3-AR is expressed in visceral adipose tissue and is thought to contribute to the regulation of resting metabolic rate and lipolysis. The missense mutation of β3-AR gene, resulting in replacement of tryptophan by arginine at position 64 (Trp64Arg), is associated with decreased resting metabolic rate and weight gain. We performed this study to determine if Trp64Arg polymorphism of β3-AR gene is associated with obesity in Koreans. Method: We investigated the relationship between the (33-AR gene mutation and body mass index (BMI), waist circumference, hip circumference, waist to hip ratio (WHR), area of subcutaneous fat, area of visceral fat, visceral to subcutaneous fat ratio (VSR), and lipid profile. 198 subjects were included in this study of which 97 were of normal weight and 101 were obese. Anthropometric data was obtained from physical examination and medical records. Result: In the cases of (33-AR gene mutation of the obese group, the ratio of Trp/Arg and Arg/Arg are 43% and 5%, respectively, which were higher than the normal group(36%, l%), although a statistical significant was not found. There was significant difference in the are of subcutaneous fat. Normal group (Trp/Trp) measured at 213.9k109.6 ㎠ versus 244.0k127.7 ㎠ (Trp/Arg) and 323.9k189.9 ㎠ (Arg/Arg) for the mutation groups. Circumference of waist, circumference of hip, WHR, area of visceral fat, and VSR were higher in the mutation groups than in normal subject, but not significantly different. Conclusion: These results suggest that a genetic mutation in the (33-AR gene can affect body fat composition, and is associated with obesity in Korean adults (J Kor SOC Endocrinol 18:184-192, 2003).

      • SCIESCOPUSKCI등재

        Expression of Latent P-Type ATPases and Their Presumptive Roles in Cell Membrane of Helicobacter pylori

        Yun,,Soon,Kyu,Hwang,,Se,Young 한국미생물 · 생명공학회 1997 Journal of microbiology and biotechnology Vol.7 No.6

        Cation motive ATPases on cell membranes of Helicobacter pylori were investigated using everted membrane vesicles. Latent ATPases could be ascertained from aggregated vesicle using N,N-dimethylformamide (DMF) and Triton X-100. By contrast, ultrasonication or chloroform treatments caused membranes to be disrupted, resulting in an alteration of sensitivities against azide or vanadate. Considerable amounts of vanadate-sensitive enzymes were identified from vesicle micelles, prepared by the dilution method. These were activated in the presence of either Ni^2+ or NH^+_4. From studies employing H. pylori intact cell systems, we found that ATPase expression of this bacterium was markedly dependent upon air composition. It was interesting that cellular expression of Ni^2+ - or NH^+_4-motive ATPases was significantly affected by extracellular pH, suggesting that these unique enzymes may physiologically he involved in cellular Ni^2+ import and NH^+_4 export, respectively

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