Circular RNA cFAM210A, degradable by HBx, inhibits HCC tumorigenesis by suppressing YBX1 transactivation

Yu Jian,Li Wen,Hou Guo-jun,Sun Da-peng,Yang Yuan,Yuan Sheng-xian,Dai Zhi-hui,Yin Hao-zan,Sun Shu-han,Huang Gang,Zhou Wei-ping,Yang Fu 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Hepatitis B protein x (HBx) has been reported to promote tumorigenesis in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC), but the mechanism awaits further investigation. In this study, we found that cFAM210A (a circular RNA derived from the third exon of transcript NM_001098801 of the FAM210A gene; CircBase ID: hsa_circ_0003979) can be silenced by HBx. cFAM210A expression was downregulated and negatively correlated with tumorigenesis in patients with HBV-related HCC. Furthermore, cFAM210A reduced the proliferation, stemness, and tumorigenicity of HCC cells. Mechanistically, HBx increased the N6-methyladenosine (m6A) level of cFAM210A by promoting the expression of RBM15 (an m6A methyltransferase), thus inducing the degradation of cFAM210A via the YTHDF2-HRSP12-RNase P/MRP pathway. cFAM210A bound to YBX1 and inhibited its phosphorylation, suppressing its transactivation function toward MET. These findings suggest the important role of circular RNAs in HBx-induced hepatocarcinogenesis and identify cFAM210A a potential target in the prevention and treatment of HBV-related HCC.

A Low-area and Low-power 512-point Pipelined FFT Design Using Radix-2⁴-2³ for OFDM Applications

Yu, Jian,Cho, Kyung-Ju Korea Information Electronic Communication Technol 2018 한국정보전자통신기술학회논문지 Vol.11 No.5

In OFDM-based systems, FFT is a critical component since it occupies large area and consumes more power. In this paper, we present a low hardware-cost and low power 512-point pipelined FFT design method for OFDM applications. To reduce the number of twiddle factors and to choose simple design architecture, the radix-$2^4-2^3$ algorithm are exploited. For twiddle factor multiplication, we propose a new canonical signed digit (CSD) complex multiplier design method to minimize the hardware-cost. In hardware implementation with Intel FPGA, the proposed FFT design achieves more than about 28% reduction in gate count and 18% reduction in power consumption compared to the previous approaches.

An area-efficient 256-point FFT design for WiMAX systems

Yu, Jian,Cho, Kyung-Ju Korea Information Electronic Communication Technol 2018 한국정보전자통신기술학회논문지 Vol.11 No.3

This paper presents a low area 256-point pipelined FFT architecture, especially for IEEE 802.16a WiMAX systems. Radix-24 algorithm and single-path delay feedback (SDF) architecture are adopted in the design to reduce the complexity of twiddle factor multiplication. A new cascade canonical signed digit (CSD) complex multipliers are proposed for twiddle factor multiplication, which has lower area and less power consumption than conventional complex multipliers composed of 4 multipliers and 2 adders. Also, the proposed cascade CSD multipliers can remove look-up table for storing coefficient of twiddle factors. In hardware implementation with Cyclone 10LP FPGA, it is shown that the proposed FFT design method achieves about 62% reduction in gate count and 64% memory reduction compared with the previous schemes.

Ethylene was Involved in Ca2+-Regulated Na+ Homeostasis, Na+ Transport and Cell Ultrastructure During Adventitious Rooting in Cucumber Explants Under Salt Stress

Yu Jian 한국식물학회 2020 Journal of Plant Biology Vol.63 No.4

Salt stress as one of main abiotic stresses damaged plant growth and development. In this study, the effects of ethylene on Ca2+-regulated rooting index, Na+ and K+ concentration, Na+ transportation and cell ultrastructure during adventitious rooting in cucumber (Cucumis sativus L.) under salt stress were investigated. The results showed that ethylene might play a positive role in Ca2+-regulated rooting index during adventitious rooting under salt stress. Moreover, CaCl2 treatment significantly decreased the intracellular Na+ level but increased K+ content of cucumber explants under salt stress. However, the Na+ and K+ concentration which treated with Ca2+ treatment was significantly influenced by ethylene inhibitors under salt stress. Our results also showed that ethylene might be involved in Ca2+-affected cell ultrastructure of cucumber explants under salt condition. Further analysis showed that ethylene might be responsible for Ca2+-regulated Na+ transport through enhancing the transcriptional and protein levels of Na+/H+ antiporter as well as H+-ATPase for alleviating the negative effects of NaCl stress. Therefore, ethylene might act as a downstream signaling molecule of Ca2+ to maintain Na+ and K+ homeostasis via regulating Na+ transport and preserve the integrity of cellular ultrastructure of cucumber explant during adventitious rooting under salt condition.

Microstructure, Thermal Conductivity and Mechanical Properties of the Mg–Zn–Sb Ternary Alloys

Yujian Huang,Xiong Zhou,Jun Du 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.11

A novel Mg alloys of Mg–xZn–ySb (x = 0, 2, 4, 6; y = 0, 0.2, 0.5, 0.8, 1.2) were designed and their microstructure, thermalconductivity and mechanical properties were systematically investigated in the present study. The as-cast Mg–Zn–Sb ternaryalloys consist of α-Mg, Mg4Zn7and Mg3Sb2phases. Sb addition can refine the eutectic structure (α-Mg + Mg4Zn7) by thegrowth of Mg4Zn7on Mg3Sb2phases. The thermal conductivity of Mg–Zn–Sb alloys decreased with increasing Sb content. There existed an interactive effect of Zn/Sb on the thermal conductivity of the Mg–Zn–Sb alloys. The negative effect of Sbaddition on thermal conductivity of alloys was getting smaller with increasing Zn content in alloys. The negative effect ofMg3Sb2phases on the thermal conductivity of alloys could be weakened by the formation of weak-scattering Mg4Zn7coatedon Mg3Sb2phases. The best refinement effect on microstructure could be obtained with 0.8 wt%Sb addition. Mg–4Zn–0.8Sballoy possess the best comprehensive properties with thermal conductivity of over than 120 W/(m·K) and UTS of 185.6 MPa.

Mixture copulas with discrete margins and their application to imbalanced data

Liu Yujian,Xie Dejun,Edwards David A.,Yu Siyi 한국통계학회 2023 Journal of the Korean Statistical Society Vol.52 No.4

This article introduces the approach of using Bayesian sampling to estimate the mixture copula with discrete margins, we further apply our models to solve the class imbalanced problems in data science by oversampling. The methodology makes it possible to learn and sample from the data set with the discrete and continuous features exists simultaneously. On the other hand, the discreetness of factors in a data set are not naturally considered for the classic SMOTE algorithm and classic random oversampling is simply performed by generating the already existing points, which do not give any new information to the classifiers and is easy to overfit. Copula methods enable us to generate new points with the correlation structure memorized by learning from the training set. Hence, the overfitting problems are reduced. Experiments with synthetic and real data are done in the article following the introduction of the methodology. The outcomes shows the validity of the approach when compared with the benchmark methods.

Fuzzy Dynamic Sliding Mode Controller Design for Uncertain Nonlinear Markovian Jump Systems

Wenqiang Ji,Yujian An,Heting Zhang 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.7

This paper investigates the problem of sliding mode control (SMC) for a class of uncertain nonlinearMarkovian jump systems through T-S fuzzy models. By adopting some convexification techniques, new results onstochastic stability analysis of the sliding motion are attained. Then two new SMC design approaches are proposedto force the closed-loop system states onto the sliding surface in finite time. Finally, two simulation examples areshown to verify the effectiveness of the proposed approaches.

Continuous Isolation of Stem-Cell-Derived Extracellular Vesicles (SC-EVs) by Recycled Magnetic Beads in Microfluidic Channels

유하은,Kim Jaejeung,YUJIANNING,Hyun Kyung-A,Lim Jae-Yol,Yoon Yeo-Jun,Park Sunyoung,Jung Hyo-Il 한국바이오칩학회 2023 BioChip Journal Vol.17 No.4

Stem cells produce nanosized particles known as extracellular vesicles (SC-EVs), which therapeutically aff ect stem cells. EVs are more abundantly produced, exhibit better stability, and possess lower immune rejection rates than stem cells. However, the traditional methods of isolating EVs, such as ultracentrifugation, possess limitations that require a complex process and consume more time. Moreover, it is diffi cult to isolate specifi c EVs that have target surface proteins that aff ect regenerative eff ects. To address these limitations, a new dual-mode horseshoe-shaped orifi ce micromixer (DM-HOMM) chip that can bind antibody-conjugated micromagnetic beads and SC-EVs and sequentially elute specifi c SC-EVs on the beads using an eluent was developed. For eff ective elution from the microbead-SC-EV complex, four types of eluents were used to control pH and ionic strength between antibodies and surface proteins in EVs. In addition, we investigated the reusability of antibody-conjugated micromagnetic beads. The beads indicated identical binding effi ciencies between the antibodies and specifi c SC-EVs for three repeated cycles using the dual-mode chip. CD63 + EVs collected by the chip exhibited higher cell viability and regeneration eff ects than untreated and total EVs. This SC-EVs’ isolation method possesses the potential for targeted therapeutic applications and enhanced regenerative effects.

Exhaust Gas Recirculation Control in a Spark-Ignition LPG Engine Using Neural Networks

Hongwei Cui,Yifang Liu,Yujian Zhai 제어로봇시스템학회 2002 제어로봇시스템학회 국제학술대회 논문집 Vol.2002 No.10

Fatigue Resistance of a BFRP-Encapsulated Long-Gauge FBG Strain Sensor under Cyclic Train Loads

Bi-tao Wu,Yujian Zhou,Huaxi Lu,Yunhuang Xiao,Zhenwei Zhou 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.9

To verify the performance of a basalt fiber reinforced polymer (BFRP) fiber-encapsulated long-gauge strain sensor in railway bridge health monitoring, this paper studies the fatigue resistance and durability of the BFRP fiber-encapsulated FBG sensor under train loads. First, the influences of the length of the anchorage section and the length ratio of the sensing section on the accuracy of the sensor were studied. Then, the BFRP sensor was applied to a sleeper for 2 million cycles of tension fatigue testing. The strain-time history of the whole fatigue test was monitored and compared. After the test, a calibration test was carried out to verify the accuracy and repeatability of the sensor. Finally, the slip and fatigue cracking of the fiber in the anchorage section of the sensor were observed by electron microscopy. The results show that the gap between the anchoring section and the bare optical fiber was filled with epoxy resin, and there was no slip behavior. No fatigue cracking occurred in the fiber, and the straincoefficient and linearity of the sensor showed no obvious changes after 2 million cycles of loading. The long-gauge strain sensor encapsulated by BFRP fibers exhibited good fatigue resistance and can meet long-term monitoring requirements under train loads.