급성 호흡곤란 증후군으로 발현된 폐결핵 1례

윤여준,박성우,이준혁,장안수,박춘식 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Pulmonary tuberculosis as a cause of the acute respiratory distress syndrome is quite rare. The diagnosis of acute respiratory distress syndrome due to pulmonary tuberculosis is difficult because of the diversity of radiological patterns. The ability to identify patients at risk for the acute respiratory distress syndrome is important if therapies are to be developed to prevent the disorder. We experienced a young female compliants with mild fever, dyspnea who diagnosed pulmonary tuberculosis 1 months ago. She stoped anti-tuberculosis medication 2 day because confirmation of drug fever and after then sudden aggravated dyspnea. The clinical course of this case is similar with presentaion of ARDS. We started antituberculosis medication with steroid, which result in good prognosis.

소득작물 전후작지 적응 극단기성 벼 ‘중모 1032’

손영보,이종희,조준현,이지윤,오성환,송유천,박동수,박노봉,김상열,여운상,남민희,권영업 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

Pretreatment with Lycopene Attenuates Oxidative Stress-Induced Apoptosis in Human Mesenchymal Stem Cells

( Ji Yong Kim ),( Jai Sung Lee ),( Yong Seok Han ),( Jun Hee Lee ),( Inhyu Bae ),( Yeo Min Yoon ),( Sang Mo Kwon ),( Sang Hun Lee ) 한국응용약물학회 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.6

Human mesenchymal stem cells (MSCs) have been used in cell-based therapy to promote revascularization after peripheral or myocardial ischemia. High levels of reactive oxygen species (ROS) are involved in the senescence and apoptosis of MSCs, causing defective neovascularization. Here, we examined the effect of the natural antioxidant lycopene on oxidative stress-induced apoptosis in MSCs. Although H2O2 (200 mM) increased intracellular ROS levels in human MSCs, lycopene (10 μmM) pretreatment suppressed H2O2-induced ROS generation and increased survival. H2O2-induced ROS increased the levels of phosphorylated p38 mitogen activated protein kinase (MAPK), Jun-N-terminal kinase (JNK), ataxia telangiectasia mutated (ATM), and p53, which were inhibited by lycopene pretreatment. Furthermore, lycopene pretreatment decreased the expression of cleaved poly (ADP ribose) polymerase-1 (PARP-1) and caspase-3 and increased the expression of B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax), which were induced by H2O2 treatment. Moreover, lycopene significantly increased manganese superoxide dismutase (MnSOD) expression and decreased cellular ROS levels via the PI3K-Akt pathway. Our findings show that lycopene pretreatment prevents ischemic injury by suppressing apoptosis-associated signal pathway and enhancing anti-oxidant protein, suggesting that lycopene could be developed as a beneficial broad-spectrum agent for the successful MSC transplantation in ischemic diseases.

Pretreatment with Lycopene Attenuates Oxidative Stress-Induced Apoptosis in Human Mesenchymal Stem Cells

Kim, Ji Yong,Lee, Jai-Sung,Han, Yong-Seok,Lee, Jun Hee,Bae, Inhyu,Yoon, Yeo Min,Kwon, Sang Mo,Lee, Sang Hun The Korean Society of Applied Pharmacology 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.6

Human mesenchymal stem cells (MSCs) have been used in cell-based therapy to promote revascularization after peripheral or myocardial ischemia. High levels of reactive oxygen species (ROS) are involved in the senescence and apoptosis of MSCs, causing defective neovascularization. Here, we examined the effect of the natural antioxidant lycopene on oxidative stress-induced apoptosis in MSCs. Although $H_2O_2$ ($200{\mu}M$) increased intracellular ROS levels in human MSCs, lycopene ($10{\mu}M$) pretreatment suppressed $H_2O_2$-induced ROS generation and increased survival. $H_2O_2$-induced ROS increased the levels of phosphorylated p38 mitogen activated protein kinase (MAPK), Jun-N-terminal kinase (JNK), ataxia telangiectasia mutated (ATM), and p53, which were inhibited by lycopene pretreatment. Furthermore, lycopene pretreatment decreased the expression of cleaved poly (ADP ribose) polymerase-1 (PARP-1) and caspase-3 and increased the expression of B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax), which were induced by $H_2O_2$ treatment. Moreover, lycopene significantly increased manganese superoxide dismutase (MnSOD) expression and decreased cellular ROS levels via the PI3K-Akt pathway. Our findings show that lycopene pretreatment prevents ischemic injury by suppressing apoptosis-associated signal pathway and enhancing anti-oxidant protein, suggesting that lycopene could be developed as a beneficial broad-spectrum agent for the successful MSC transplantation in ischemic diseases.

IBC microbial agent formulation technique development

Yeo Jun Yoon,Su Jin Kim,Min Hyun Kim,Je Kyung Park 한국응용곤충학회 2022 한국응용곤충학회 학술대회논문집 Vol.2022 No.04

Evaluation of Surgical Outcomes of Single port Versus Multiport Robotic myomectomy: matched- case control study

( Jun Young Yeo ),( Hyun Jung Lee ),( Yoon Hee Lee ),( Gun Oh Chong ),( Dae Gy Hong ),( Yoon Soon Lee ) 대한산부인과학회 2019 대한산부인과학회 학술대회 Vol.105 No.-

Objective: To compare the surgical outcomes of robotic-assisted myomectomy with the s single port or multiport approach. Methods: Comparison of consecutive single port robotic myomectomy undertaken between 2017 and 2018 with multiport robotic myomectomy completed between 2010 and 2018 by the same surgeon. 58 patients with single port robotic myomectomy were matched at the uterine tumor level to 58 patients with multiport myomectomy. Results: The total operative time, estimated blood loss, conversion rate, hospital stays. CRP and Hb change, number of myoma, weight of myoma and surgical complications were compared between the 2 study groups. One hundred sixteen patients underwent robotic-assisted myomectomy (58 single port and 58 multiport). There was no significant difference between the single port and multiport approach in weight (227.4 ±374.3 vs. 234.2±417.6 gm. P=0.743) and number of myoma (1.6±1.2 vs. 2±1.6, P=0.074), estimated blood loss (50±86.2 vs. 62.6±94.6 ml, P=0.163) and postoperative change of Hb(-1.3±1.1 vs. -1.6±1.2 g/dL, P=0.202) and CRP(3.8±3.3 vs. 3.3±2.8 mg/dL, P=0.599). There was no conversion to multiport in single port group. However, the operation time was shorter in the single port group(102.2±37 vs. 165.7±68.7 min, P= <0.001). There were many complications in the mutiport group (14% vs. 5%, P=0.002). The period of hospitalization were shorter in the single port group (3.8±1.8 vs. 4.5±1.4 days, P = 0.004). Odds ratios and 95% confidence intervals were calculated from multivariable logistic regression models. This result showed only statistical difference in operation times (Odd ratio=0.972, P<0.001), postoperative CRP changes (Odd ratio=1.261, P=0.024). Conclusion: Even though there were technical difficulties in single port robot myomectomy. However, our results were rather encouraging in selected patients; compare to multiport robotic myomectomy, single port robotic myomectomy had definite advantages in operation times, hospital stay and cosmetic benefits.

Hemorrhagic Stroke and Blindness after Hyaluronic Acid/Polylactic Acid Filler Injection

Yeo Jun Yoon,Doyeon Kook,Hye Sun Choi,Jiyun Lee,Yo Han Jung 대한신경초음파학회 2023 대한신경초음파학회지 (JNN) Vol.15 No.1

The number of people receiving facial filler injections is increasing as it is relatively safe than the other cosmetic procedures. However, complications of facial filler injection range from mild redness and inflammation to cerebral infarction. We report the case of a patient who was diagnosed with cerebral infarction and intracranial hemorrhage after facial filler injection. A 61-year-old male presented to the emergency department with ocular pain and visual loss in the left eye after filler injection into the glabella. Neurological examination revealed monocular blindness. Initial brain magnetic resonance imaging (MRI) revealed small, acute multifocal infarctions in the left frontal, parietal, and temporal lobes. Follow-up MRI revealed subarachnoid hemorrhages in the left frontal and parietal lobe. Follow-up brain computed tomography showed small intracerebral hemorrhage in the left frontal lobe. Clinicians must be aware that not only cerebral infarction, but intracranial hemorrhage could also be a potential adverse effect of the cosmetic filler injection in the face.

The Effect of Low Frequency Electromagnetic Fields on the Proliferation of vestibular schwann cells

Yeo Jun Yoon,In Seok Moon,Gwan Soo Park,Jong Dae Lee,Kiwon Song 대한두개저학회 2016 대한두개저학회지 Vol.11 No.2

Objectives : Authors reported that the time of mobile phone usage can be related with the vestibular schwannoma growth. In this study, we tried to approach to the biological effect of the extremely low frequency electromagnetic field (ELF-EMF) on NF2-/- mouse schwann (SC4) cells. Experimental Design : We used SC4 cell line and optimized the ELF-EMF exposure conditions with the ELF-EMF exposure results of other cells. For acute response, we exposed 1 and 10mT ELF-EMF to SC4 cells for 30minutes. For continuous exposures, SC4 cell were incubated in the 1 and 10mT ELF-EMF up to 144 hr. Cell viability was measured by MTT assays and proteins were detected by western blots. Distribution of cells in each stage of the cell cycle was analyzed by Fluorescence-activated cell sorting (FACS). Results : SC4 cells exposed to the continuous as well as the acute ELF-EMF did not show any DNA double strand breaks and apoptosis up to 144 hr. Interestingly, the proliferation of SC4 cells was increased by continuous exposures to ELF-EMF in an intensity and dose dependent manner. However, no activation of Erk and Akt pathways was observed in these cells. Moreover, both the EMF-exposed and unexposed SC4 cells showed similar distribution of cells in each stage of the cell cycle. Conclusion : These results demonstrated that ELF-EMF of the similar total value of multiplied energy and exposure time of RF-EMF can accelerate the proliferation of SC4, indirectly supporting a positive correlation between the cell phone usage and the tumor size.

The control of sweet potato whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae) by the entomopathogenic fungi, Lecanicillium (Verticillium) lecanii Btab01

Yeo-jun Yoon,Jong-ho Park,Eun-jung Han,Min-ho Lee,Yong-man Yu 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10

Sweet potato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is a serious pest of many economically important crops. The insect has developed resistance to chemical insecticides. Therefore, the development of microbial agent such as entomopathogenic fungi, Lecanicillium lecanii is necessary. Spores of L. lecanii Btab01 was collected after cultivation on solid PDA and liquid RB (rice bran amended with 2% molasses) media. The bioassay was carried out with B. taabci nymphs for 7 days at 25℃ and 60% relative humidity. Further, mortality was corrected with appropriate controls. The results revealed that spores obtained from RB medium caused high mortality (98.31%) compared to PDA medium (93.94%). Spore concentrations 105, 106, 107, and 108 colony forming units (c. f. u) ml-1 caused 5.81, 33.80, 77.27, and 98.54% mortality, respectively. The mortality (100%) was observed for 4 days when L. lecanii spores was mixed with 0.1 - 0.3% soybean oil. Hence, it is concluded from this study, L. lecanii Btab01 cultivated on RB medium can be recommended to control the nymphs of B. tabaci. Spore suspension can be expected to high efficacy when soybean oil was blended.

A hepatocyte growth factor/MET-induced antiapoptotic pathway protects against radiation-induced salivary gland dysfunction

Yoon, Yeo Jun,Shin, Hyun-Soo,Lim, Jae-Yol Elsevier 2019 Radiotherapy and oncology Vol.138 No.-

<P><B>Abstract</B></P> <P><B>Objective</B></P> <P>Hepatocyte growth factor (HGF) and its receptor MET are expressed in the salivary glands during developmental stages and tumor formation; however, the function of HGF in injured salivary gland tissues remains unclear. The present study investigated the role of HGF in protecting the salivary glands against radiation-induced injury using an organotypic culture method.</P> <P><B>Materials and methods</B></P> <P>Acinar-like organoids were formed by means of a three-dimensional (3D) human parotid tissue-derived spheroids (hPTS) culture method. Radioprotective effects of HGF on irradiated hPTS and signaling pathways on radioprotection were investigated.</P> <P><B>Results</B></P> <P>We detected MET expression in hPTS grown in a 3D culture. Treatment of irradiated hPTS with recombinant human HGF (rhHGF) restored salivary marker expression and secretory function of hPTS. Changes in the phosphorylation levels of apoptosis-related proteins through HGF-MET axis inhibited radiation-induced apoptosis. Treatment with PHA665752, a MET inhibitor, blocked MET-PI3K-AKT pathway, increased apoptosis, and suppressed the radioprotective effect of rhHGF against IR-induced damage of hPTS.</P> <P><B>Conclusions</B></P> <P>These results suggest that HGF is a key effector of radioprotection and that HGF-MET-PI3K-AKT axis is involved in protecting the salivary glands from radiation-induced apoptosis.</P> <P><B>Highlights</B></P> <P> <UL> <LI> HGF rescues the structure and function of irradiated human parotid epithelial cells. </LI> <LI> HGF increases antiapoptotic protein levels in irradiated cells. </LI> <LI> HGF acts as a key effector of radioprotection by modulating MET-PI3K-AKT pathway. </LI> </UL> </P>