Association Study of Single-Nucleotide Polymorphisms of STAT2/STAT3/IFN-γ Genes in Cervical Cancer in Southern Chinese Han Women

Yuan, Yuan,Fan, Jie-Lin,Yao, Fang-Ling,Wang, Kang-Tao,Yu, Ying,Carlson, Jennifer,Li, Ming Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.8

Objective: Interferon-${\gamma}$ (IFN-${\gamma}$) and signal transducers and activators of transcription (STATs) each play an important role in carcinogenesis associated with viral infection. Cervical cancer is almost invariably associated with infection by human papillomavirus (HPV), and previous studies suggested that dysregulation of the signal pathway involved in IFN-${\gamma}$ and STATs is associated. Our objective was to evaluate the association of SNPs in STAT2, STAT3, and IFN-${\gamma}$ with cervical cancer susceptibility in Chinese Han women in Hunan province. Materials and Methods: Genomic DNA was extracted from peripheral blood samples of 234 cervical cancer patients and 216 healthy female controls. STAT2 and STAT3 genotyping was performed using polymerase chain reaction-restriction enzyme (PCR-RE) analysis. IFN-${\gamma}$ genotyping was detected by PCR-amplification of specific allele (PASA). Results: For STAT2 rs2066807 polymorphisms, there was no significant difference of genotype distribution (P=0.827) and allele frequencies (P=0.830, OR=1.09, 95% CI: 0.51-2.31) between cases and controls. For STAT3 rs957970 polymorphisms, there was no significant difference of genotype distribution (P=0.455) and allele frequencies (P=0.560, OR=0.92, 95% CI: 0.71-1.20) between cases and controls. For IFN-${\gamma}$ +874A/T polymorphisms, there was no significant difference of genotype distribution (P=0.652) and allele frequencies (P=0.527, OR=1.12, 95% CI: 0.79-1.59) between cases and controls. Conclusion: These results suggest that polymorphisms in STAT2, STAT3 and IFN-${\gamma}$ genes are not likely to be strong predictors of cervical cancer in Han women in southern China.

A 20–40 SECOND PROCESS FOR SYNTHESIS OF MULTI-WALLED CARBON NANOTUBES

YUAN-YAO LI,TING-CHI LIU,CHIH-CHE HSIEH,MASAKI SAGEHASHI,AKIYOSHI SAKODA 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2006 NANO Vol.1 No.2

We report on a 20–40 s process for growing aligned, uniformed, high purity multi-walled carbon nanotubes (MWNTs) using an alcohol lamp burner. With an appropriate catalyst precursor concentration, MWNTs can be grown on a Co-coated 304 stainless steel mesh under incomplete combustion conditions at 680–700°C. The process provides a rapid, easy, economic and safe method for the formation of carbon nanotubes. We suggest that the proposed method be considered as an educational kit for college or high school level experiments.

Luminescent characteristics of Sr2ZnSi2O7 : Eu3+ phosphor for ultraviolet light emitting diodes

Shan-shan Yao,Li-hong Xue,You-wei Yan,Yuan-yuan Li,Mi-fang Yan 한양대학교 세라믹연구소 2010 Journal of Ceramic Processing Research Vol.11 No.6

Red-emitting phosphors Sr2-xZnSi2O7 : Eux 3+ was prepared by a combustion-assisted synthesis method and an efficient red emission under near-ultraviolet (UV) was observed. The luminescence, crystallinity, and particle sizes were investigated using luminescence spectrometry, X-ray diffractometry, and field scanning electron microscopy. The emission spectrum shows that the most intense peak is located at 614 nm, which corresponds to the 5D0→ 7F2 transition of Eu3+. The phosphor has two main excitation peaks located at 394 and 465 nm, which match the emission of UV and blue light-emitting diodes, respectively.

Efficacy and Safety of Neurokinin-1 Receptor Antagonists for Prevention of Chemotherapy-Induced Nausea and Vomiting: Systematic Review and Meta-analysis of Randomized Controlled Trials

Yuan, Dong-Mei,Li, Qian,Zhang, Qin,Xiao, Xin-Wu,Yao, Yan-Wen,Zhang, Yan,Lv, Yan-Ling,Liu, Hong-Bin,Lv, Tang-Feng,Song, Yong Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.4

Objectives: Can addition of neurokinin-1 receptor antagonists (NK1-RAs) be considered as an ideal strategy for the prevention of chemotherapy-induced nausea and vomiting (CINV)? Researchers differ on this question. Materials and Methods: Electronic databases were searched for randomized control trials (RCTs) that evaluated the effectiveness and safety of NK1-RAs in preventing CINV. The primary end point was complete response (CR) in the acute, delayed, and overall phases after chemotherapy. Subgroup analyses evaluated the types of NK1-RAs, routines of administration, types of malignancies, regimens used in combination with NK1-RAs, and age of patients included in the studies. The incidences of different types of adverse events were also extracted to estimate the safety of NK1-RAs. Results: A total of 38 RCTs involving 13,923 patients were identified. The CR rate of patients receiving NK-RAs was significantly higher than patients in the control groups during overall phase (70.8% vs 56.0%, P<0.001), acute phase (85.1% vs 79.6%, P<0.001), and delayed phase (71.4% vs 58.2%, P<0.001). There were three studies including patients of children or adolescents, the CR rate was also significantly higher in the treatment group (overall phase: OR=2.807, P<0.001; acute phase: OR=2.863, P =0.012; delayed phase: OR=2.417, P<0.001). For all the other outcomes, patients in the NK1-RAs groups showed improvements compared to the control groups (incidence of nausea: 45.2% vs 45.9%, P<0.001; occurrence of vomiting: 22.6% vs 38.9%, P<0.001; usage of rescue drugs: 23.5% vs 34.1%, P<0.001). The pooled side effects from NK1-RAs did not significantly differ from previous reports and the toxicity rates in patients less than eighteen years old also did not diff between the two groups (P=0.497). However, we found that constipation and insomnia were more common in the patients of control groups, whereas diarrhea and hiccups were more frequently detected in patients receiving NK1-RAs. Conclusions: NK1-RAs improved the CR rate of CINV. They are effective for both adults and children. The use of NK1-RAs might be associated with the appearance of diarrhea and hiccups, while decreasing the possibility of constipation and insomnia.

Thermal-fluid-structure coupling analysis on plate-type fuel assembly under irradiation. Part-II Mechanical deformation and thermal-hydraulic characteristics

Li, Yuanming,Ren, Quan-yao,Yuan, Pan,Su, Guanghui,Yu, Hongxing,Zheng, Meiyin,Wang, Haoyu,Wu, Yingwei,Ding, Shurong Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.5

The plate-type fuel assembly adopted in nuclear research reactor suffers from complicated effect induced by non-uniform irradiation, which might affect stress conditions, mechanical behaviors and thermal-hydraulic performance of the fuel assembly. This paper is the Part II work of a two-part study devoted to analyzing the complex unique mechanical deformation and thermal-hydraulic characteristics for the typical plate-type fuel assembly under irradiation effect, which is on the basis of developed and verified numerical thermal-fluid-structure coupling methodology under irradiation in Part I of this work. The mechanical deformation, thermal-hydraulic performance and Mises stress have been analyzed for the typical plate-type fuel assembly consisting of support plates under non-uniform irradiation. It was interesting to observe that: the plate-type fuel assembly including the fuel plates and support plates tended to bend towards the location with maximum fission rate; the hot spots in the fuel foil appeared at the location with maximum thickness increment; the maximum Mises stress of fuel foil was located at the adjacent location with the maximum plate thickness increment et al.

A clinical Comparison of Lobaplatin or Cisplatin with Mitomycine and Vincristine in Treating Patients with Cervical Squamous Carcinoma

Li, Wei-Ping,Liu, Hui,Chen, Li,Yao, Yuan-Qing,Zhao, En-Feng Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.11

Background: The research was to compare the efficacy and side effects of cisplatin or lobaplatin in combination with mitomycine (MMC) and vincristine in treating patients with cervical squamous carcinoma. Materials and Methods: Cervical squamous carcinoma patients who were pathologically diagnosed with stage Ib-IIb from April 2012 to May 2013 in the general hospital of Chinese People's Libration Amy were enrolled. All patients were confirmed without prior treatment and were randomly divided into two groups, Group A and B. Efficacy and side effects were evaluated after one cycle of chemotherapy. Results: Group A (n=42) were treated with Loubo$^{(R)}$ (Lobaplatin) $50mg/m^2$, MMC $16mg/m^2$ and Vincristine $2mg/m^2$ every 21 days. Group B (n=44) were treated with Cisplatin $100mg/m^2$, MMC $16mg/m^2$ and Vincristine $2mg/m^2$ every 21 days. All 86 patients completed one cycle of chemotherapy with cisplatin or lobaplatin in combination with MMC and vincristine. No difference was observed regardiing short-term effect between two groups. Main side effects were bone marrow suppression and gastrointestinal reactions including decrease of white blood cells, platelet and nausea/vomiting. Grade III-VI liver and kidney impairment was not reported in two groups. In group A the incidence of uterine artery spasm in the process of drug delivery was significantly lower than the group B. Conclusions: Cisplatin or lobaplatin with MMC and Vincristine in the interventional treatment of cervical squamous carcinoma were effective, especially after uterine artery perfusion chemotherapy at tumor reduction and tumor downstaging period. The adverse reactions of concurrent chemotherapy are tolerable, and low physical and mental pressure even more less stimulation of vascular in treatment with lobaplatin. However, the long-term effects of this treatment need further observation.

Efficient quantum secure communication scheme with one-time pad

Li, Yuan,Zeng, Guihua,Yao, Weili,Lee, Moonho Springer-Verlag 2009 Optical review Vol.16 No.3

Thermal-fluid-structure coupling analysis for plate-type fuel assembly under irradiation. Part-I numerical methodology

Li, Yuanming,Yuan, Pan,Ren, Quan-yao,Su, Guanghui,Yu, Hongxing,Wang, Haoyu,Zheng, Meiyin,Wu, Yingwei,Ding, Shurong Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.5

The plate-type fuel assembly adopted in nuclear research reactor suffers from complicated effect induced by non-uniform irradiation, which might affect its stress conditions, mechanical behavior and thermal-hydraulic performance. A reliable numerical method is of great importance to reveal the complex evolution of mechanical deformation, flow redistribution and temperature field for the plate-type fuel assembly under non-uniform irradiation. This paper is the first part of a two-part study developing the numerical methodology for the thermal-fluid-structure coupling behaviors of plate-type fuel assembly under irradiation. In this paper, the thermal-fluid-structure coupling methodology has been developed for plate-type fuel assembly under non-uniform irradiation condition by exchanging thermal-hydraulic and mechanical deformation parameters between Finite Element Model (FEM) software and Computational Fluid Dynamic (CFD) software with Mesh-based parallel Code Coupling Interface (MpCCI), which has been validated with experimental results. Based on the established methodology, the effects of non-uniform irradiation and fluid were discussed, which demonstrated that the maximum mechanical deformation with irradiation was dozens of times larger than that without irradiation and the hydraulic load on fuel plates due to differential pressure played a dominant role in the mechanical deformation.

Wnt/β-Catenin Promotes the Osteoblastic Potential of BMP9 Through Down-Regulating Cyp26b1 in Mesenchymal Stem Cells

Yao Xin-Tong,Li Pei-pei,Liu Jiang,Yang Yuan-Yuan,Luo Zhen-Ling,Jiang Hai-Tao,He Wen-Ge,Luo Hong-Hong,Deng Yi-Xuan,He Bai-Cheng 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.5

BACKGROUND: All-trans retinoic acid (ATRA) promotes the osteogenic differentiation induced by bone morphogenetic protein 9 (BMP9), but the intrinsic relationship between BMP9 and ATRA keeps unknown. Herein, we investigated the effect of Cyp26b1, a critical enzyme of ATRA degradation, on the BMP9-induced osteogenic differentiation in mesenchymal stem cells (MSCs), and unveiled possible mechanism through which BMP9 regulates the expression of Cyp26b1. METHODS: ATRA content was detected with ELISA and HPLC–MS/MS. PCR, Western blot, and histochemical staining were used to assay the osteogenic markers. Fetal limbs culture, cranial defect repair model, and micro–computed tomographic were used to evaluate the quality of bone formation. IP and ChIP assay were used to explore possible mechanism. RESULTS: We found that the protein level of Cyp26b1 was increased with age, whereas the ATRA content decreased. The osteogenic markers induced by BMP9 were increased by inhibiting or silencing Cyp26b1 but reduced by exogenous Cyp26b1. The BMP9-induced bone formation was enhanced by inhibiting Cyp26b1. The cranial defect repair was promoted by BMP9, which was strengthened by silencing Cyp26b1 and reduced by exogenous Cyp26b1. Mechanically, Cyp26b1 was reduced by BMP9, which was enhanced by activating Wnt/b-catenin, and reduced by inhibiting this pathway. b-catenin interacts with Smad1/5/9, and both were recruited at the promoter of Cyp26b1. CONCLUSIONS: Our findings suggested the BMP9-induced osteoblastic differentiation was mediated by activating retinoic acid signalling, viadown-regulating Cyp26b1. Meanwhile, Cyp26b1 may be a novel potential therapeutic target for the treatment of bone-related diseases or accelerating bone-tissue engineering.

Diversity and geographic variation of endosymbiotic bacteria in natural populations of the pea aphid (Acyrthosiphon pisum) in China

Zhang Li,Cao Ya,Zhang Li-Jun,Wang Meng-Yao,Wang Xiao-Tong,Yang Xing-Zhuo,Duan Ting-Yu,Yuan Ming-Long 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3

Bacterial symbionts in aphids are known to benefit the insect host and associated with aphid’s ecological adaptation. The pea aphid (Acyrthosiphon pisum), an important legume pest worldwide, carries at least eight endosymbionts, providing a model system to study insect–bacteria interactions. However, species diversity and geographic variations of endosymbionts are unknown in Chinese populations; therefore, we characterized symbiont communities and diversity of 27 pea aphid samples from 13 geographic populations of China. Via amplicon high-throughput sequencing and diagnostic PCR, we found that bacterial communities of Chinese populations were dominated by Proteobacteria and Firmicutes. Among eight known endosymbionts, five (Buchnera, Serratia, Hamiltonella, Regiella, and Rickettsia) were detected by both methods, with a specific geographical distribution. The obligate symbiont, Buchnera, was present in all aphid samples, while the four facultative symbionts showed a significant geographic variation. Each population was randomly infected with distinct endosymbionts, ranging from three to five species. Serratia and Rickettsia showed relatively higher abundance in central regions of China, Regiella was predominant in eastern and western China, whereas Ham iltonella showed an extremely low abundance and was absent in four populations. Samples grouped by altitudes showed a significant diversity difference, whereas there was no significant difference between red and green body colors. Bacterial community structures of the Chinese pea aphid populations were mainly influenced by environmental factors, other than body colors. These data can guide the development of potential biocontrol techniques against this aphid.