A Study of the relationship between Chronic Pain and Quality of Life for Elderly in Long-term Care Service - Focused on the Mediating Effect of Depression -

Yu-me No,Jeoung-nam Yang 한국융합학회 2018 한국융합학회논문지 Vol.9 No.4

본 연구는 장기요양 재가서비스 이용 노인의 만성통증이 삶의 질에 미치는 영향에서 우울의 매개효과를 검증하는 데 목적을 두었다. 이를 위해 장기요양 재가서비스를 이용하고 있는 노인 204을 대상으로 조사를 실시하였다. 본 연구의 주요 분석결과는 변수간의 상관관계에서 만성통증과 우울은 정적관계, 만성통증과 삶의 질과의 관계에서는 부적 상관관계 로 분석되었다. 또한, 만성통증과 우울이 삶의 질에 미치는 영향에 있어서 우울은 완전한 매개효과를 보이는 것으로 검증되 었다. 이 결과를 통해 장기요양 재가서비스를 이용하는 노인의 다양한 융합적인 정신건강서비스를 위한 프로그램 개발 필요성에 대해 논의하였다. In this study, the effect of chronic pain on the lives of elderly people in long-term care service was analyzed based on the mediated effect of depression. The research data was sampled from elderly people in long-term care services, 204 people participated. From mediated regression analysis, depression was the most relevant factor on the quality of life, followed by chronic pain. With chronic pain and depression as independent variables and quality of life as a dependent variable, depression was proved to have had a fully mediated effect on quality of life. The result of this study suggested that convergence of various support systems should be implemented for the elderly in long-term care services.

Molecular analysis of transgenic rice overexpressing UDP-glycosyltransferase from Brassica rapa

Me-Sun Kim,Hye-Jung Lee,Joonki Kim,Dal-A Yu,Sanguk Byeon,Kwon-Kyoo Kang,Illsup Nou,Yong-Gu Cho 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

Secondary plant metabolites undergo several modification reactions, including glycosylation and physiological functions. Glycosylation, which is mediated by UDP-glycosyltransferase (UGT), plays a role in the storage of secondary metabolites and in defending plants against stress. In this study, a UDP-glucosyltransferase cDNA was isolated from Brassica rapa hereinafter referred to as BrUGT. It has a full-length cDNA of 1,236 bp that contains a single open reading frame of 834 bp which encodes a polypeptide of 277 amino acid residues with a calculated mass of 31.19 kDa. BLASTX analysis hits a catalytic domain of glycos_transf_1 super family (c112012) that belongs to the glycosyltransferases group 1 with tetratricopeptide (TPR) regions. UGT gene expression analysis showed high mRNA transcripts in pistil, followed by petal, seed and calyx of flower in Brassica rapa. Furthermore, we constructed a recombinant pFLCIII vector carrying the BrUGT gene under the control of ubiquitin promoter and NOS terminator and transformed into rice using Agrobacterium tumefaciens. The UGT overexpressing rice lines were then characterized at the physiological and molecular levels. To further understand the biological function of BrUGT, transcriptional profiling of the gene in transgenic rice lines under cold, salt, PEG, H2O2, ABA and drought stress condition is underway.

Classification of Korean rice varieties based on growth characteristics

Me-Sun Kim,Hye-Jung Lee,Dal-A Yu,Joonki Kim,Franz Nogoy,Eun-Ju Jeong,Jang-Hwan You,Yong-Gu Cho 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

The International Union for the Protection of New Varieties of Plants (UPOV) promotes an effective system of plant variety protection and encourages the development of new varieties of plants. International convention was initiated to standardized the system efforts and strengthen the policy. This study was conducted to establish a database for rice identification using morphological characters which include number of tillers and panicle per plant, spikelets per panicle, yield, plant maturity, height, leaf pigments, flag leaf angles, and rice bran. The whole rice population was grouped into three based on leaf angles, majority members of which retained the flag leaf angle-character until maturity stage. Most rice accessions did not exhibit anthocyanin pigments on the leaves particularly on the first leaf, leaf blade, leaf sheath and auricle, except for varieties classified as black rice. In the case of grain, many accessions produced secondary branching, and showed no awn. For agronomic traits, productive tiller and panicle per plant were higher in early flowering varieties, while spikelets per panicle and ripened grain were higher in late flowering varieties, and yield was higher in medium flowering varieties. All data were then pooled for cluster analysis which revealed three major independent clusters and four minor clusters.

Overexpression of CIPK 15 improved tolerance to pre-harvest sprouting in rice

Dal-A Yu,Hye-Jung Lee,Joonki Kim,Me-Sun Kim,Marjohn Nino,Sothea Ouk,Seong-Dong Kim,Ill-sup Nou,Yong-Gu Cho 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

Since global climate changes drastically, pre-harvest sprouting (PHS) is expected to pose serious problems in rice production. CBL-interacting serine/threonine protein kinases (CIPKs) have been implicated to play important role in regulating various abiotic stresses such as cold, salinity and drought. In this study, to understand the function of this gene under pre-harvest sprouting in rice, a cDNA clone encoding CBL-interacting protein kinase 15 was isolated from rice flowers. We constructed a recombinant vector carrying the CIPK15 under the control of the CaMV 35S promoter and Tnos terminator and transformed into rice using Agrobacterium tumefaciens. Insertion of the gene was verified in transformants using HPT resistance test and genomic PCR. Transcriptional profiling using tissues of wild type, Gopum, revealed expression of the gene in whole plant tissues with level of expression highest in the seeds suggesting possible role in dormancy. Comparative expression analysis of the gene in transgenic and wild type through semi-quantitative RT-PCR and real-time PCR showed higher expression in transgenic rice lines. Moreover, screening in the mist chamber showed overexpression lines that were resistant to the PHS. This result suggests the involvement of CIPK15 in the regulation of pre-harvest sprouting.

Overexpression of CBL-interacting protein kinase 15 improves tolerance to pre-harvest sprouting in rice (Oryza sativa L.)

Dal-A Yu,Hye-Jung Lee,Joonki Kim,Me-Sun Kim,Marjohn Nino,Sanguk Byeon,Jeongmi Kim,Illsup Nou,Kwon-Kyoo Kang,Yong-Gu Cho 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

Since global climate changes drastically, pre-harvest sprouting (PHS) is expected to pose serious problems in rice production. CBL-interacting serine/threonine protein kinases (CIPKs) have been implicated to play important role in regulating various abiotic stresses such as cold, salinity and drought. In this study, to understand the function of this gene under pre-harvest sprouting in rice, a cDNA clone encoding CBL-interacting protein kinase 15 (CIPK15) was isolated from rice flowers. This gene is 2,818 bp long with 1,332 bp coding region that encodes a polypeptide of 443 amino acids. We constructed a recombinant vector carrying the OsCIPK15 under the control of the CaMV 35S promoter and Tnos terminator and transformed into rice using Agrobacterium tumefaciens. Insertion of the gene was verified in transformants using HPT resistance test and genomic PCR. Transcriptional profiling using tissues of wild type, Gopum, revealed expression of the gene in whole plant tissues with level of expression highest in the seeds suggesting possible role in dormancy. Comparative expression analysis of the gene in transgenic and wild type through semi-quantitative RT-PCR and real-time PCR showed higher expression in transgenic rice lines. Moreover, screening in the mist chamber showed overexpression lines that were resistant to the PHS. This result suggests the involvement of OsCIPK15 in the regulation of pre-harvest sprouting.

Modification of starch composition using RNAi targeting of SSS1 gene in rice

Hye-Jung Lee,Moo-Geun Jee,Dal-A Yu,Me-Sun Kim,Joonki Kim,Seon-Kyeong Song,Kwon-Kyoo Kang,Yong-Gu Cho 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

An increasing preference for good eating quality of rice among consumers has become one of the important considerations in rice breeding. Amylose content is a leading factor affecting eating quality of rice. Amylose composition is determined by the relative activity of soluble starch synthase (SSS) and granule-bound starch synthase (GBSS). This study focused on modifying the expression of SSSI gene which is responsible for amylopectin and amylose synthesis in rice by using RNA interference (RNAi) technology. The transgenic rice plants showed various amylose content in rice grains. Favorable rice lines were selected according to genomic PCR, transgene expression and amylose contents analysis. A semi-quantitative RT-PCR was carried out to determine the expression level of SSSI gene after flowering of transgenic rice and wild type. Down-regulation of SSSI gene in transgenic plants was evident in the decreasing expression in rice grains. Accordingly, scanning electron microscopy (SEM) analysis revealed uniform size with smooth curves starch granules in down-regulation rice lines, in contrast with the non-uniform granules in wild type. Results indicated that RNAi-SSSI transgenic lines produced low amylose contents that fell between glutinous and non-glutinous rice. This study showed that down-regulation of endogenous SSSI may improve the eating quality in rice.

Modification of Starch Composition Using Downregulation of GBSS1 gene in Japonica Rice

Hye-Jung Lee,Moo-Geun Jee,Dal-A Yu,Me-Sun Kim,Franz Nogoy,Mingmao Sun,Sailila E. Abdula,Kwon-Kyoo Kang,Illsup Nou,Yong-Gu Cho 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

There is a great consideration on rice eating quality aside from improving its tolerance to various stresses. High yielding and pest and disease tolerant rice is highly desirable but it is more commercially important if it also has a high eating quality. There are various factors contributing to the good eating quality of rice. This study focuses on modifying the expression of GBSS1 genes which are responsible for amylopectin and amylose synthesis in rice by using RNAi and antisense techniques. We have developed 40 transgenic plants with RNAi-GBSS1 gene and 60 transgenic lines with antisense-GBSS1 gene. The transgenic plants show diverse amylose contents in rice seed. We selected candidate lines according to PCR, RNA expression and amylose contents. A semi-quantitative RT-PCR was carried out to measure the expression level of GBSS1 gene at several time points after the flowering of transgenic plants. The expression level of GBSS1 gene in rice grains decreases over time and the mRNA expression among the transgenic plants were lower compare to its wild type. In the SEM analysis, the starch granule of wild type Gopumbyeo has very large structures accompanied with small ones around the area. However, the starch structures in transgenic plants were smaller and more uniform in size and shape throughout the viewing area

십이지장경 소견으로 진단이 가능했던 췌장의 Mucinous Ductal Ectasia 3예

김명환,박선미,서동진,이성구,최호순,민영일,송일한,이미화,유은실,유미란,한덕종,공경엽,이문규 대한소화기내시경학회 1995 Clinical Endoscopy Vol.15 No.2

We have experienced three cases of mucinous ductal ectasia of the pancreas. They showed the characteristic duodenoscopic findings and underlying pathology was hyperplasia in two cases and adenocarcinoma in one case. When endoscopic retrograde pancreatography was performed, bulging ampulla of Vater, patulous ampullary orifice and mucus leakage from papillary orifice were noted. Also cyst-like dilatation of main duct or side branch of the uncinate process were observed.

III 급 부정교합의 치료

유필식,김동훈,김중한,이승미,김미애,박관수,박형수,임한호,윤영주,김광원 朝鮮大學校 口腔生物學硏究所 2000 Oral Biology Research (Oral Biol Res) Vol.24 No.2

Class Ⅲ malocclusion occurs in the number of patients of Asians and is one of the most difficult types of malocclusion to treat Class Ⅲ malocclusion primarily results from maxillary skeletal retrusion and mandibular skeletal protrusion, and combination of both. Treatment of Class Ⅲ malocclusion usually includes growth modification (Fra¨nkel. Face mask, Chin cup etc.), comprehensive orthodontic therapy, either combined with extraction and/or orthognathic surgery. This study describes the diagnosis and treatment methods of Class Ⅲ malocclusion with the illustrations of clinical cases.

The process of National Health Insurance coverage for Chuna Manual Therapy in Korea: A qualitative study

Yu Jieun,Shin Byung-Cheul,Kim Hyunmin,Hyun Eunhye,Lee Jin-Ho,Kim Me-Riong,Lim Byungmook 한국한의학연구원 2022 Integrative Medicine Research Vol.11 No.1

Background: Chuna Manual Therapy (CMT) has been widely used in Korea, and coverage in Korean National Health Insurance (NHI) was finally implemented in 2019. The objectives of this study were to analyze the process of NHI coverage for CMT qualitatively, and to summarize important roles, streams, and implications regarding its inclusion in the modern public health insurance system. Methods: Related literature was collected and 8 key personnel involved in the policy-making process were qualitatively interviewed, and Zahariadis’ version of the Multiple Streams Framework (MSF) was applied to analyze the policy agenda setting and the roles of stakeholders. Results: Through the collaborative efforts of various stakeholders, a pilot coverage project for CMT was implemented in 2017, and coverage was expanded nationwide in 2019. MSF showed that it was mainly achieved through three streams: governmental change (political stream), demand from the general public and KM doctors (problem stream), and strengthening/reinforcement of the feasibility and acceptability of the policy (policy steam). Also, the roles of policy entrepreneurs and resulting changes were shown to be significant for the overall process. Conclusion: NHI coverage for CMT was realized through collective policy and research efforts from the government and academic sectors. The roles of stakeholders were shown to be significant in the overall process, and documentation of their involvement is hoped to be of use of other countries that utilize traditional and/or manual medicine. Background: Chuna Manual Therapy (CMT) has been widely used in Korea, and coverage in Korean National Health Insurance (NHI) was finally implemented in 2019. The objectives of this study were to analyze the process of NHI coverage for CMT qualitatively, and to summarize important roles, streams, and implications regarding its inclusion in the modern public health insurance system. Methods: Related literature was collected and 8 key personnel involved in the policy-making process were qualitatively interviewed, and Zahariadis’ version of the Multiple Streams Framework (MSF) was applied to analyze the policy agenda setting and the roles of stakeholders. Results: Through the collaborative efforts of various stakeholders, a pilot coverage project for CMT was implemented in 2017, and coverage was expanded nationwide in 2019. MSF showed that it was mainly achieved through three streams: governmental change (political stream), demand from the general public and KM doctors (problem stream), and strengthening/reinforcement of the feasibility and acceptability of the policy (policy steam). Also, the roles of policy entrepreneurs and resulting changes were shown to be significant for the overall process. Conclusion: NHI coverage for CMT was realized through collective policy and research efforts from the government and academic sectors. The roles of stakeholders were shown to be significant in the overall process, and documentation of their involvement is hoped to be of use of other countries that utilize traditional and/or manual medicine.