COMBINATORIAL PROPERTIES OF NONNEGATIVE MATRICES

Shao, Jia Yu TOPOLOGY AND GEOMETRY RESEARCH CENTER 1993 Proceedings of the Topology and Geometry Research Vol.3 No.-

Rapid and Efficient Detection of 16SrI Group Areca Palm Yellow Leaf Phytoplasma in China by Loop-Mediated Isothermal Amplification

Shao-shuai Yu,Hai-yan Che,Sheng-jie Wang,Cai-li Lin,Ming-xing Lin,Wei-wei Song,Qing-hua Tang,Wei Yan,Wei-quan Qin 한국식물병리학회 2020 Plant Pathology Journal Vol.36 No.5

Areca palm yellow leaf (AYL) disease caused by the 16SrI group phytoplasma is a serious threat to the development of the Areca palm industry in China. The 16S rRNA gene sequence was utilized to establish a rapid and efficient detection system efficient for the 16SrI-B subgroup AYL phytoplasma in China by loopmediated isothermal amplification (LAMP). The results showed that two sets of LAMP detection primers, 16SrDNA-2 and 16SrDNA-3, were efficient for 16SrIB subgroup AYL phytoplasma in China, with positive results appearing under reaction conditions of 64oC for 40 min. The lowest detection limit for the two LAMP detection assays was the same at 200 ag/μl, namely approximately 53 copies/μl of the target fragments. Phytoplasma was detected in all AYL disease samples from Baoting, Tunchang, and Wanning counties in Hainan province using the two sets of LAMP primers 16SrDNA-2 and 16SrDNA-3, whereas no phytoplasma was detected in the negative control. The LAMP method established in this study with comparatively high sensitivity and stability, provides reliable results that could be visually detected, making it suitable for application and research in rapid diagnosis of AYL disease, detection of seedlings with the pathogen and breeding of diseaseresistant Areca palm varieties.

Leaky Gut in IBD: Intestinal Barrier–Gut Microbiota Interaction

Yu Shunying,Sun Yibin,Shao Xinyu,Zhou Yuqing,Yu Yang,Kuai Xiaoyi,Zhou Chunli 한국미생물·생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.7

Inflammatory bowel disease (IBD) is a global disease that is in increasing incidence. The gut, which contains the largest amount of lymphoid tissue in the human body, as well as a wide range of nervous system components, is integral in ensuring intestinal homeostasis and function. By interacting with gut microbiota, immune cells, and the enteric nervous system, the intestinal barrier, which is a solid barrier, protects the intestinal tract from the external environment, thereby maintaining homeostasis throughout the body. Destruction of the intestinal barrier is referred to as developing a “leaky gut,” which causes a series of changes relating to the occurrence of IBD. Changes in the interactions between the intestinal barrier and gut microbiota are particularly crucial in the development of IBD. Exploring the leaky gut and its interaction with the gut microbiota, immune cells, and the neuroimmune system may help further explain the pathogenesis of IBD and provide potential therapeutic methods for future use.

Event-triggered Control of Positive Systems With State Saturation Using Linear Programming

Yu Shao,Junfeng Zhang,Laiyou Liu,Xuanjin Deng 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.1

This paper proposes the event-triggered control of positive systems with state saturation both in discretetime and continuous-time cases. A 1-norm based event-triggered mechanism is established for positive systems. Under the event-triggered mechanism, the error term between actual and sample states is transformed into intervaluncertain form. Together with the properties of saturation, the systems with state saturation are transformed intointerval uncertain systems and the corresponding lower and upper bounds of the system matrices are obtained,respectively. Using a linear co-positive Lyapunov function, the event-triggered controller and the auxiliary gainmatrix of the domain of attraction are designed in terms of linear programming, respectively. Then, the systemswith state and input saturation are also described via interval uncertain systems. An event-triggered controller isdesigned and thus the closed-loop systems are positive and stable under the designed controller. Furthermore, thepresented event-triggered control approach is extended to the continuous-time case. Compared to existing controlapproaches, the event-triggered control can reduce energy consumption and increase the practicability. Finally,several numerical examples are given to illustrate the effectiveness of the proposed design

Downregulation of the long noncoding RNA MBNL1-AS1 protects sevoflurane-pretreated mice against ischemia-reperfusion L216injury by targeting KCNMA1

Shao-Nan Yu 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Total knee arthroplasty (TKA) is the most common and cost-effective treatment for older adults with long-standing osteoarthritis. During TKA, muscle cells suffer from prolonged oxygen deficiency, which leads to altered cell metabolism that reduces the energy demand and maintains cell homeostasis before blood flow is restored. This study focused on the role of the lncRNA muscleblind-like 1 antisense RNA 1 (MBNL1-AS1) in protecting sevofluranepretreated mice against ischemia-reperfusion (I/R) injury after TKA, as well as the elucidation of the potential associated mechanism. Identification of differentially expressed lncRNAs was performed using the microarray dataset GSE21164, which was extracted from the GEO database. Target genes of the lncRNA were determined using Multi-Experiment Matrix (MEM), a dual-luciferase reporter gene assay, and KEGG enrichment analyses. The results showed that MBNL1- AS1 was overexpressed in skeletal muscle cells in mice, while KCNMA1, which was enriched in the cGMP-PKG signaling pathway, was negatively regulated by MBNL1-AS1. Furthermore, I/R mice displayed serious inflammatory reactions. Down-regulation of MBNL1-AS1 increased the expression of KCNMA1, PKGII, VASP, VEGF, Bcl-2, Cyclin D1, Cyclin D3, and Cdc 42 but decreased the expression of Bax, cleaved caspase-3, and cleaved PARP. Furthermore, upon MBNL1-AS1 upregulation, the rate of cell apoptosis increased while the rate of cell proliferation decreased. Our data suggested that down-regulated lncRNA MBNL1-AS1 might promote the proliferation and inhibit the apoptosis of skeletal muscle cells by upregulating KCNMA1 expression via activation of the cGMP-PKG signaling pathway, thus protecting sevofluranepretreated mice against I/R injury after TKA.

Generalized exponents of non-primitive graphs

Shao, Jia-Yu,Hwang, Suk-Geun 경북대학교 위상수학 기하학연구센터 1999 硏究論文集 Vol.8 No.-

The exponent of a primitive digraph is the smallest integer k such that for each ordered pair of (not necessarily distinct) vertices x and y there is a walk of length k from x to y. As a generalization of exponent, Brualdi and Liu (Linear Algebra Appl. 14 (1990) 483-499) introduced three types of generalized exponents for primitive digraphs in 1990. In this paper we extend their definitions of generalized exponents from primitive digraphs to general digraphs which are not necessarily primitive. We give necessary and sufficient conditions for the finiteness of these generalized exponents for graphs (undirected, corresponding to symmetric digraphs) and completely determine the largest finite values and the exponent sets of generalized exponents for the class of non-primitive graphs of order n, the class of connected bipartite graphs of order n and the class of trees of order n. ⓒ 1998 Elsevier Science Inc. All rights reserved.

Human lactoferrin efficiently targeted into caprine beta-lactoglobulin locus with transcription activator-like effector nucleases

Yu-Guo Yuan,Shao-Zheng Song,Meng-Ming Zhu,Zheng-Yi He,Rui Lu,Ting Zhang,Fei Mi,Jin-Yu Wang,Yong Cheng 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.8

Objective: To create genetically modified goat as a biopharming source of recombinant human lacotoferrin (hLF) with transcription activator-like effector nucleases. Methods: TALENs and targeting vector were transferred into cultured fibroblasts to insert hLF cDNA in the goat beta-lactoglobulin (BLG) locus with homology-directed repair. The gene targeted efficiency was checked using sequencing and TE7I assay. The bi-allelic gene targeted colonies were isolated and confirmed with polymerase chain reaction, and used as donor cells for somatic cell nuclear transfer (SCNT). Results: The targeted efficiency for BLG gene was approximately 10%. Among 12 Bi-allelic gene targeted colonies, five were used in first round SCNT and 4 recipients (23%) were confirmed pregnant at 30 d. In second round SCNT, 7 (53%), 4 (31%), and 3 (23%) recipients were confirmed to be pregnant by ultrasound on 30 d, 60 d, and 90 d. Conclusion: This finding signifies the combined use of TALENs and SCNT can generate bi-allelic knock-in fibroblasts that can be cloned in a fetus. Therefore, it might lay the foundation for transgenic hLF goat generation and possible use of their mammary gland as a bioreactor for large-scale production of recombinant hLF.

Resource-Optimal Licensed-Assisted Access in Heterogeneous Cloud Radio Access Networks With Heterogeneous Carrier Communications

Lien, Shao-Yu,Cheng, Shin-Ming,Chen, Kwang-Cheng,Kim, Dong In IEEE 2016 IEEE Transactions on Vehicular Technology VT Vol.65 No.12

<P>To support a tremendous amount of traffic demands via wireless access in 2020 and beyond, limited bandwidth of the licensed bands has been a major obstacle to boosting further the capacity of wireless services. To fundamentally break through this predicament, an emerging technology known as heterogeneous carrier communications has been launched into standardization in the form of licensed-assisted access (LAA) to the unlicensed bands. Integrating the heterogeneous cloud radio access networks and carrier aggregation, and although LAA-empowered cellular networks gain wider bandwidth from the unlicensed bands, communications may suffer from intersystem interference. To avoid interference, listen-before-talk has been designated as a mandatory function; however, it leads to significant challenges of the hidden-terminal problem. To address this open issue in LAA, in this paper, we consequently propose a resource-optimal scheme using a minimum amount of replicated radio resources to achieve the most essential latency guarantees for real-time applications. To further support non-real-time applications, a new resource control as well as the mathematical architecture inspired by so-called political communications is proposed to furthermaximize the throughput of packet delivery. Our scheme not only optimizes resource utilization in time and spatial domains but also suggests optimum energy efficiency and computation efficiency, to successfully deploy cellular networks on the unlicensed bands.</P>

Improving Cellulase Production in Trichoderma koningii Through RNA Interference on ace1 Gene Expression

( Wang Shao Wen ),( Miao Xing ),( Gang Liu ),( Shao Wen Yu ),( Juan Wang ),( Sheng Li Tian ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.8

Ribonucleic acid interference (RNAi) inhibits the expression of target genes in a sequence-specific manner, and shows potential for gene knockdown in filamentous fungi, in which the locus-specific gene knockout occurs in low frequency. In this study, the function of the repressor of cellulase expression I (ACEI) was verified in Trichoderma koningii (T. koningii) YC01 through RNAi, and ace1-silenced strains with improved cellulase productivity were obtained. An expression cassette that transcribed the interfering double-stranded RNA (dsRNA) of ace1 was constructed and transformed into T. koningii, and the transformants, in which the expression of ace1 was successfully silenced, were selected. As a result of the ace1 gene silencing, the expression levels of the main cellulase and xylanase genes were elevated, and the enhanced production of total proteins, cellulase, and xylanase was observed in the cultivation. In addition, the downregulation of ace1 resulted in an increasing expression of xyr1, but no clear variation in the expression of cre1, which suggested that ACEI acted as a repressor of the xyr1 transcription, but was not involved in the regulation of the cre1 expression. The results of this work indicate that ace1 is a valid target gene for enhancing enzyme production in T. koningii, and RNAi is an appropriate tool for improving the properties of industrial fungi.

중국 애니메이션에서 색채 상징성 연구

샤오쉬원 ( Shao Yu Wen ),김해윤 ( Kim Hae Yoon ) 한국기초조형학회 2020 한국기초조형학회 학술발표논문집 Vol.2020 No.1