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Plant Regeneration from Protoplasts of Solanum tuberosum and S. stoloniferum
Yousef Hamidoghli,Kenneth Ward 한국원예학회 2006 Horticulture, Environment, and Biotechnology Vol.47 No.1
Pot plant materials of cultivated potato Solanum tuberusom cv. Aula and wild species S. stoloniferum were established from tubers and subsequently transferred to tissue culture conditions. One of the main difficulties with cv. Aula protoplast regeneration was the production and oxidation of phenolic compounds, causing browning. Initial culture of protoplasts using liquid medium and weekly replacement with double the volume of fresh medium, starting from the first division, completely overcame the browning problem. After 3 days of the culture, protoplasts of cv. Aula from newly established tissue culture shoots started to bud instead of dividing. The amount of budding in protoplasts isolated from shoots after 3?4 passages in culture was greatly reduced. It is recommended that when starting with in vivo plant materials, several passages during in vitro culture are necessary prior to protoplast culture. During this period, the plants might become adapted to in vitro conditions.
Ali Reza Noroozi Sharaf,Yousef Hamidoghli,Hedayat Zakizadeh 한국원예학회 2011 Horticulture, Environment, and Biotechnology Vol.52 No.3
Primula heterochroma is a scarce and endemic plant to the north of Iran. The aim of the present study is to establish an efficient and reproducible protocol for in vitro propagation from shoot tips. Native seeds were exposed to different treatments. Germination results showed that the scarification of seeds had significant effects on the germination delay and germination percentage compared to plant growth regulator treatments. Afterwards, the seedling-derived shoot tips were cultured on several Murashige and Skoog basal medium, containing various concentrations of 6-benzylaminopurine (BA) or thidiazuron (TDZ) in combination with various concentrations of 1-naphthaleneacetic acid (NAA). The result showed that the highest shoot regeneration, and the number and length of leaves were noticeably affected by media enriched with 1 mg・L^(-l) BA + 0.4 mg・L^(-l) NAA and 0.2 mg・L^(-l) TDZ + 0.4 mg・L^(-1) NAA. Root parameters significantly affected by media enriched with 1 mg・L^(-1) BA + 0.8 mg・L^(-1) NAA and 0.4 mg・L^(-1) TDZ + 0.8 mg・L^(-1) NAA. Plantlets were hardened-off and transferred to soil for further growth.
Sara Nikkhah Bahrami,Hedayat Zakizadeh,Yousef Hamidoghli,Mahmood Ghasemnezhad 한국원예학회 2013 Horticulture, Environment, and Biotechnology Vol.54 No.6
The effect of exogenous salicylic acid (SA) on the vase life of cut lisianthus flowers was investigated. ‘Miarichi Grand White’ flowers were obtained from a commercial grower and were pulse-treated with a 0, 50, 100, 150or 200 mg·L-1 SA solution containing 200 mg·L-1 8-hydroxyquinoline sulphate (HQS) and 5% (w/v) sucrose for 18 hours. As compared to distilled water (DW) as the control, the greatest delay in petals senescence was obtained in cut flowers pulsed with 100 mg·L-1 SA. Vase life of flowers increased by 4.5 days in the above mentioned treatment. The characteristics such as water uptake (WUP), relative fresh weight (RFW), protein concentration, proline accumulation,and activities of lipoxygenase (LOX) and superoxide dismutase (SOD) were measured in the 0 and 100 mg·L-1 SA treatments over the period of vase life. The WUP and RFW were obviously greater in the SA-treated flowers than in the control. Degradation of protein and accumulation of proline during vase life was shown to be suppressed by 100 mg·L-1 SA. The activity of LOX was gradually increased with progressing flower senescence, while the activity of SOD was declined. The flowers which had been pulsed with 100 mg·L-1 SA exhibited lower LOX and a higher SOD activities. Overall, the results suggest that SA can increase the vase life of lisianthus cut flowers by improving the antioxidant system and reducing damages caused by oxidative stress during senescence.
Yahya Tajvar,Reza Fotouhi Ghazvini,Yousef Hamidoghli,Reza Hassan Sajedi 한국원예학회 2011 Horticulture, Environment, and Biotechnology Vol.52 No.6
The production of reactive oxygen species (ROS) in plants under low temperature stress causes damage to plants. In order to evaluate the antioxidant responses of Thomson navel young trees (two years old) on different rootstocks under low temperatures, an experiment was conducted in a factorial plan based on completely randomized design. Treatments of temperatures were at seven levels including 9, 6, 3, 0, -3, -6℃, and 25 ± 2℃ (as control) and rootstocks were Sour orange,Citrange and Trifoliate orange. Results of interactive effects of factors showed that electrolyte leakage (EL), antioxidant capacity, catalase (CAT) and ascorbate peroxidase (APX) activity were significant at P < 0.01. Maximum EL was observed in Thomson navel on Sour orange at -6℃, while the highest antioxidant capacity, CAT and APX activity respectively with mean 75.06%, 0.45 and 4.350 IU・g-1 FW were observed in Thomson navel on Trifoliate orange rootstock at 0, -3 and 0℃. Low temperatures increased EL, lipid peroxidation, antioxidant capacity, superoxide dismutase (SOD), APX, CAT and peroxidase enzyme activities (P < 0.01). Due to effects of rootstock, lipid peroxidation reduced to 52 % (P < 0.01). The biochemical and physiological study of this experiment indicated that Thomson navel on Trifoliate orange rootstock, had best tolerance to freezing stress.