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Reza Fotouhi Ghazvini,Maryam Tatari Varnusfaderani,Yousof Hamidoghli 한국원예학회 2005 Horticulture, Environment, and Biotechnology Vol.46 No.5
This study was conducted on in vitro induction of bulblets from bulb scale explants of Lilium ledebourii and Lilium × Gironde (Asiatic hybrid). Apical and basal segments of bulb scales were cut separately and cultured aseptically on MS basal medium supplemented with different concentrations of 6-benzyladenine (BA) at 0.01 and 0.1 ㎎ㆍℓ?¹ and α-naphthaleneacetic acid (NAA) at 0.01 and 0.1 ㎎ㆍℓ?¹ and on a medium without plant growth regulators. The greater number and fresh weight of L. ledebourii bulb lets were obtained on medium without plant growth regulators. For Asiatic hybrid, however, the greater number and fresh weight of bulb lets (9.16 and 1.1g, respectively) increased on medium containing BA plus NAA at equal concentrations of 0.1 ㎎ㆍℓ?¹. In both species, the highest number of roots was obtained on medium containing 0.1㎎ㆍℓ?¹ NAA without BA. Basal parts of bulb were more effective than the apical sections.
Effect of Water Stress on Freezing Tolerance of Mexican Lime (Citrus aurantifolia L.) Seedlings
Reza Fotouhi Ghazvini,Mohammad Reza Baghbanha,Abdollah Hatamzadeh,Mokhtar Heidari 한국원예학회 2008 Horticulture, Environment, and Biotechnology Vol.49 No.5
Mexican lime (Citrus aurantifolia L.) is the most sensitive citrus plant to freezing. To study the effect of water stress on development of freezing tolerance of lime seedlings, changes of chlorophyll content, total soluble sugar, electrolyte leakage (EL), proline, and chlorophyll fluorescence parameters, as indicators of freezing damage were evaluated. The seedlings at the stage of 7-9 leaves were exposed to four levels of field capacity (FC) including 100%, 75%, 50%, and 25% FC. Then, water stressed plants were subjected to -2, -4, -6, and -8℃ freezing temperatures for 12 hours. The results showed an increase in EL and total soluble sugar during freezing stress, whereas chlorophyll content, efficiency of photosystem II (Fv/Fm), and electron transfer rate (ETR) considerably decreased. Within the water stressed treatments, 25% FC decreased EL to 71.5% of control, while chlorophyll content, soluble sugars, Fv/Fm, and ETR were significantly increased. Therefore, as a vital indicator, 25% FC water stress could improve freezing tolerance of lime seedlings.
Hamid Reza Rouhi Ghorabaie,Reza Fotouhi Ghazvini,Behrouz Golein,Ali Reza Nabipour 한국원예학회 2010 Horticulture, Environment, and Biotechnology Vol.51 No.4
Understanding phylogenetic relationships and genetic diversity in Citrus are important in clarifying genetic relationships, characterizing germplasm and the registration of new cultivars. There are some Citrus accessions in Iranian citrus collections which have been classified merely based on their morphological traits. Molecular markers would help to infer their relations with known cultivars. In this study 30 Citrus accessions and eight known cultivars from Kotra collection were investigated using 11 pairs of SSR markers, which in total produced 63 alleles with an average of 5.72 alleles per locus. The lowest number of alleles was observed in cAGG9 locus with only 2 alleles and the highest number of alleles was observed in TAA41 locus with 10 alleles. All loci had pretty high PIC with an average of 0.571. The highest PIC was related to TAA41 locus with 0.745. A similarity dendrogram was constructed using Complete Linkage clustering method which put the 38 samples into 5 major groups.
Yahya Tajvar,Reza Fotouhi Ghazvini,Yousef Hamidoghli,Reza Hassan Sajedi 한국원예학회 2011 Horticulture, Environment, and Biotechnology Vol.52 No.6
The production of reactive oxygen species (ROS) in plants under low temperature stress causes damage to plants. In order to evaluate the antioxidant responses of Thomson navel young trees (two years old) on different rootstocks under low temperatures, an experiment was conducted in a factorial plan based on completely randomized design. Treatments of temperatures were at seven levels including 9, 6, 3, 0, -3, -6℃, and 25 ± 2℃ (as control) and rootstocks were Sour orange,Citrange and Trifoliate orange. Results of interactive effects of factors showed that electrolyte leakage (EL), antioxidant capacity, catalase (CAT) and ascorbate peroxidase (APX) activity were significant at P < 0.01. Maximum EL was observed in Thomson navel on Sour orange at -6℃, while the highest antioxidant capacity, CAT and APX activity respectively with mean 75.06%, 0.45 and 4.350 IU・g-1 FW were observed in Thomson navel on Trifoliate orange rootstock at 0, -3 and 0℃. Low temperatures increased EL, lipid peroxidation, antioxidant capacity, superoxide dismutase (SOD), APX, CAT and peroxidase enzyme activities (P < 0.01). Due to effects of rootstock, lipid peroxidation reduced to 52 % (P < 0.01). The biochemical and physiological study of this experiment indicated that Thomson navel on Trifoliate orange rootstock, had best tolerance to freezing stress.
Micropropagation from Stem Segments of Salt Tolerant Jojoba Seedlings
Hosein Meyghani,Reza Fotouhi Ghazvini,Yousof Hamidoghli 한국원예학회 2005 Horticulture, Environment, and Biotechnology Vol.46 No.3
In order to produce shoots from nodal segments of salt tolerant jojoba seedlings, the established explants on MS basal medium containing benzyladenine (BA) and naphthaleneacetic acid (NAA), were transferred to 1/2 MS medium supplemented with BA and NAA, kinetin and NAA, and BA, kinetin, and NAA at different concentrations. The number of shoots produced on the medium with 2 ㎎ㆍℓ?¹ BA and 0.1 ㎎ㆍℓ?¹ NAA (5.0 shoots per explant in average) was higher than the other treatments. Rooting of in vitro shoots was carried out on MS basal medium containing indole-3-butyric acid (IBA) and NAA at different concentrations. The highest rate of rooting occurred when either shoots were first treated with 120 ㎎ㆍℓ?¹ IBA solution for 24 h followed by transferring into the MS medium without plant growth regulators or cultured on MS medium supplemented with 4 ㎎ㆍℓ?¹ IBA and 2 ㎎ㆍℓ?¹ NAA. More roots were produced on MS medium containing both IBA and NAA than when they were used alone. The mixtures of peat and perlite at a ratio of 1:1 or 1:2 (v/v) were selected as the most suitable media for transplanting or adaptation of jojoba plantlets.