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생인공치주인대 제조를 위한 차폐막이 부착된 복합 나노섬유 스캐폴드의 제조
서영권 ( Young Kwon Seo ),안재일 ( Jae Il Ahn ),최경민 ( Kyung Min Choi ),윤희훈 ( Hee Hoon Yoon ),송계용 ( Kye Yong Song ),김영진 ( Young Jin Kim ),최정원 ( Jeong Won Choi ),손영숙 ( Young Sook Son ),박정극 ( Jung Keug Park ) 한국조직공학과 재생의학회 2006 조직공학과 재생의학 Vol.3 No.4
Various biodegradable scaffolds have been used for the development of bioartificial periodontal ligament( Bio-PDL), but those scaffolds were focused on cell growth and differentiation only, and could not function as a guided tissue regeneration(GTR) membrane which can inhibit gingival cells migration. So, in this study, we tried to make a composite scaffold composed of collagen nanofiber on amniotic membrane(AM) using electrospinning for dental tissue engineering. As results, we could make a composite scaffold whose breaking load was 1±0.3 N superior to nanofiber scaffold without AM(0.1±0.05 N). After seeding of PDL cell on each scaffold, they attached and proliferated well on both scaffolds. However nanofiber only scaffold was contracted after 5 days of culture, but composite nanofiber scaffold was not contracted for all culture days. In conclusion, the composite nanofiber scaffold made by electrospun collagen fiber on AM will be a suitable substrate for PDL cell culture finally resulting in a good Bio-PDL.
서영권 ( Young Kwon Seo ),안재일 ( Jae Il Ahn ),이두훈 ( Doo Hoon Lee ),권순용 ( Soon Yong Kwon ),정도현 ( Do Hun Jung ),박용순 ( Yong Soon Park ),송계용 ( Kye Yong Song ),양은경 ( Eun Kyung Yang ),김영진 ( Young Jin Kim ),박정극 한국조직공학과 재생의학회 2004 조직공학과 재생의학 Vol.1 No.2
Amniotic membrane (AM) dressing can be used for the protection against superficial bacterial infection, facilitation of reepithelialization, reduction of caloric and fluid loss from burn or ulcers. In addition, it increases the speed of healing and minimizes the scarring of tissue repair of wounds. Objective of this study is to evaluate the wound healing effect of deepithelialized human AM with keratinocytes on it and the usefulness as substrates of epithelial cells culture. Deepithelialized AM was made by removal of their amniotic epithelial cells with 0.05% trypsin treatment and lyophilization and compared the wound healing effect with the intact human AM with keratinocytes. First, we evaluated the antiinflammatory effect of human AM, which was mixed with collagen solution in a powder form and freeze dried. Second, we cultured keratinocytes onto deepithelialized AM and grafted to the rat skin defect in full-thickness, and then the epidermal regeneration and dermal fibrosis was compared. As results, AM showed antiinflammatory effects evidence by much lowered inflammatory cell infiltrates in the implantation sites. Keratinocytes were cultured better and stratified on the deepithelialized AM than intact AM. Epithelial regeneration and fibrosis was also much better than intact AM. Therefore, we can conclude that AM had antiinflammatory effects, improve the wound healing and also suitable for cell culture substrates.
Application of Nutrient Enriched Media on Organ Culture of Human Scalp Hair Follicle
Bo-Young Yoo(유보영),Hee-Hoon Yoon(윤희훈),Doo-Hoon Lee(이두훈),Young-Kwon Seo(서영권),Youn-Ho Shin(신연호),Kye-Yong Song(송계용),Sung-Joo Hwang(황성주),Young-Jin Kim(김영진),Eun-Kyung Yang(양은경),Chang-Seo Park(박장서),Ih-Seop C 한국생물공학회 2004 한국생물공학회 학술대회 Vol.2004 No.4
Development of In vitro 3-D Human Hair Growth Model using Dermal Equivalent
Bo-Young Yoo(유보영),Hee Hoon Yoon(윤희훈),Doo-Hoon Lee(이두훈),Young-Kwon Seo(서영권),Youn-Ho Shin(신연호),Kye-Yong Song(송계용),Sung-Joo Hwang(황성주),Young-Jin Kim(김영진),Eun-Kyung Yang(양은경),Chang-Seo Park(박장서),Ih-Seop C 한국생물공학회 2004 한국생물공학회 학술대회 Vol.2004 No.10