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Young Chan Cho(曺永璨),Tae Young Chung(鄭泰英),Yong Hwan Park(朴用煥),Hak Soo Suh(徐學洙) 한국육종학회 1995 한국육종학회지 Vol.27 No.1
Genetic variation and phylogenetic relationship among twenty four strains of Korean red rice, one foreign red rice and five cultivars (Oryza sativa L.) were investigated at the DNA level by using randomly amplified polymorphic DNA (RAPD) method. Twenty of 23 10-mer primers assayed, produced strong and reproducible DNA bands. The number of amplified bands from each 10-mer primer varied from 2 to 8, with an average of 5.3 bands per primer. However, an average of 0.5 band per primer was polymorphic between a Korean red rice strain and a japonica cultivar. Out of 103 different bands observed among thirty accessions by using fifteen primers, 85(82.5%) were informative to differentiate one or more accessions for the others. Particularly, many polymorphic bands were detected among accessions which detected few polymorphism by RFLP analysis. A dendrogram of the phylogenetic similarities among thirty Korean red rices and cultivars was constructed based on their DNA polymorphisms. Classification of rice accessions based on the results from RAPD analysis was differentiated into two main groups, short grain red rices which include japonica cultivars and long grain red rices which include indica and Tongil-type cultivars. This result was in accordance with previous classifications based on isozyme and RFLP analysis. From genetic analysis of F₁ and F₂ populations of a cross between a japonica cultivar, Nagdongbyeo and a Korean red rice strain with short grain type, Geumleungaengmi 33, RAPD polymorphic bands were dominant and inherited following the Mendelian ratio.
1939년 조선일보 주최 조선주택설계도안 현상모집에 관한 고찰
김용범,박용환,Kim, Young-Bum,Park, Yong-Hwan 한국건축역사학회 2008 건축역사연구 Vol.17 No.2
This study examines the details and characteristics of prizewinning plans of Cho-Sun housing plan competition promoted by Daily Cho-Sun Company during Japanese colonial period. The main features of Cho-Sun housing plan competition were that the competition held with an application of popular newspaper readers and a judging committee was composed of only Korean architects, especially Park, Gil-Ryong as the president of the board of examiners. Cho-Sun housing plan competition put ideological factors for the improvement of living as like advancing to the cultured(modem) life, respecting for family life, respecting for privacy of family members and so on. First of all, the competition suggested the direction of the improvement of dwelling spaces which focused on making harmonious home life. This study found common characteristics of prizewinning plans of Cho-Sun housing plan competition as the result. First, Cho-Sun housing plan competition introduced a centralized house plan as a model of the improvement of small-sized houses. Second, architectural orders of western or Japanese style mingled with ones of korean traditional style in prizewinning plans as entrance hall, western style reception room(parlour), maid's room, bathroom for example. And a manner of seating using chairs was brought into the style of living which would be assumed in the prizewinning plans. Third, traditional way of composing dwelling spaces was maintained, receiving the characteristics of dwelling spaces of japanese style simultaneously, which central corridor was put at the heart of house for improving arrangement of rooms and flow lines. Fourth, lavatory and bathroom were placed inside of the house.
김영미 ( Young Mi Kim ),손성한 ( Seong Han Sohn ),정순일 ( Soon Il Jeong ),윤문섭 ( Mun Sup Yoon ),김태산 ( Tae San Kim ),박용환 ( Yong Hwan Park ) 한국응용생명화학회 2002 Applied Biological Chemistry (Appl Biol Chem) Vol.45 No.4
Along with the worldwide rapid increase of the cultiation area and commercial production of genetically modified (GM) crops, the amount of Gm grains imported to Korea has also been increasing. Roundup-Ready soybean (RRS) was introduced with 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) gene derived from Agrobacterium CP4 to confer the resistance to herbicide, glyphosate. In this study, we tried to develop PCR-based analytical method to detection the presence of RRS among non-GM soybeans. In order to detect RRS specifically, oligonucleotide primers were specifically designed based on the nucleotide sequence of E{S{S transgene. Qualitative PCR method was established and its specificity and accuracy were confirmed by analysing the nucleotide sequence of PCR DNA fragments. Bioassay was also conductted by spraying glyphosate at seedling stage. Survived individuals showed obvious resistance to Roundup Ready, however all of non-GM seedlings died in two weeks after spray. Conclusively, the highly selective detection systems for RRS were successfully established by both PCR using specific primers to EPSPS transgene and bioassay using the herbicide resistance of RRS. In addition to, the imported soybean showed to be mixed to several varieties regarding to 100-seed weight and hilum color.
부직포 및 알루미늄 적층이 소방용 방열복의 방열 방화 성능에 미치는 영향
김영수(Young-Soo Kim),박평규(Pyoung-Kyu Park),박용환(Yong-Hwan Park) 한국위험물학회 2014 한국위험물학회지 Vol.2 No.2
Protective clothing with heat and flame resistance for fire fighting is typically heavy, thick and bulky. High density woven fabrics of spunlace nonwoven and needle punching nonwoven were tested respectively to increase comfort and light-weight. The fabrics of single and dual mirror aluminized film were also tested for heat radiation protection, showing that better performance for double mirror fabric rather than single mirror fabric when exposed to medium density heat flux environment. Thicker fabric with needle punching nonwoven showed better thermal protective performance than that with spunlace nonwoven. Single or dual mirror aluminized film layer had little effect on TPP, however, had significant effect on HTI due to the high reflectance in IR region.
유영복,유창현,박용환,죤 페버디,Yoo, Young-Bok,You, Chang-Hyun,Park, Yong-Hwan,Peberdy, John F. The Korean Society of Mycology 1986 韓國菌學會誌 Vol.14 No.1
느타리버섯과 사철느타리버섯의 영양요구성 균주를 원형질체융합에 의하여 이종간융합균주를 선발하였다. 이 체세포잡종을 재배하여 자실체에서 담자포자를 분리하여 life cycle에 대한 유전분석을 한 결과를 요약하면 다음과 같다. 1. $Arg{\times}Ribo-1,\;Arg{\times}Ribo-2,\;Gly\;Ser{\times}Ribo-1$ 그리고 $Gly\;Ser{\times}Ribo-2$ 4개의 융합조합에서 7개의 융합균주를 분석한 결과 모두 유전형질이 분리되었으며 또한 유전자 조환이 일어났다. $Arg{\times}Ribo-1$과 $Arg{\times}Ribo-2$에서는 4종류로 분리되었으며 $Gly\;Ser{\times}Ribo-1$, $Gly\;Ser{\times}Ribo-2$와 같이 대립유전자가 3인자 일때는 8종류로 기대되나 단지 2내지 6종류로만 분리 되었다. 2. 대림유전자의 비에 었어서 $Arg{\times}Ribo-1$과 $Arg{\times}Ribo-2$에서 1:1로 기대되나 Ribo의 분리수가 많은 3:1 으로 분리되었으며, $Gly\;Ser{\times}Ribo-1$과 $Gly\;Ser{\times}Ribo-2$에서는 1:1:1과는 아주 상이한 분리비를 나타내었다. 3. 4개의 융합조합 모두 연관에 속하지 않았으며 조환가는 $38.7{\sim}67.6%$였다. 4. 분리되는 prototrophic recombinants의 균사체에서 clamp connection이 형성되었는데 4개의 융합조합에서 모두 나타났으며 융합균주에 따라 차이가 났는데 $Arg{\times}Ribo-1$의 p5균주는 곁정된 수의 78.5%가 clamp connection을 형성하였다. 또한 이들 균주의 자실체는 정상이었으며 자실체의 색은 느타리버섯과 사철느타리버섯의 중간이었다. Interspecific hybrids of Pleurotus ostreatus and Pleurotus florida were formed by using protoplasts of complementing auxotrophs. The genetic markers were shown to segregate and recombine in the first generation of monosporus isolates from basidiocarp of seven fusion products. The analysis provides proof of heterokaryosis and strong evidence for haploidy of vegetative nuclei, a sexual cycle consisting of nuclear fusion and meiosis. In all the crosses there was no evidence of linkage between the genetic markers. Clamp connections were formed in monosporus mycelia from basidiocarp of fusion products.
일제강점기 근대주택 평면변천의 사상적 배경에 관한 연구
손영민(Son Young-Min),김용범(Kim Young-Bum),박용환(Park Yong-Hwan) 대한건축학회 2010 대한건축학회논문집 Vol.26 No.9
This paper is review about a Movement of Living Development and a Discourse of Housing Improvement during the Japanese occupation period. While Movement of Living Development began in the 1920s. Korean architects were efforting to Improve conventional house. And they suggested plan to develop housing for that time. That plan show theirs thinking and thinking process about improving housing. I had collected various publications such as a newspaper and magazine. And then I had examined the contents of a related article. Through these actions, I analyzed the ideology of Housing Improvement and the characteristics of the Planned Housing that appears in the Improved Housing Proposal. Korean Architects were studying new change housing model to solve the problem of unreasonable conventional housing. New change housing model was reflected Modern housing ideas such as a cultural life, respecting for family life, respecting for privacy of family members, the sense of economy, hygienic awareness. Especially, While maintain the traditional way spatial configuration of conventional house, through the introduction of entrance hall and corridor, they wanted to improve room layout and user's movements. And they tried to design improving housing of modern style through the introduction of compromise between Western style and Japanese style such as a living room, a maid's room, a washstand and a bathroom.