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Yoshikazu Hayashi,Masahiro Okada,Takaaki Morikawa,Tatsuma Nomura,Hisashi Fukuda,Takahito Takezawa,Alan Kawarai Lefor,Hironori Yamamoto 대한소화기내시경학회 2021 Clinical Endoscopy Vol.54 No.3
Superficial colonic neoplasms sometimes extend into a diverticulum. Conventional endoscopic mucosal resection of these lesionsis considered challenging because colonic diverticula do not have a muscularis propria and are deeply inverted. Even if the solutionis carefully injected below the mucosa at the bottom of the diverticulum, the mucosa is rarely elevated from the diverticular orifice,and it is usually just narrowed. Although endoscopic submucosal dissection or full-thickness resection with an over-the-scope clipdevice enables the complete resection of these lesions, it is still challenging, time consuming and expensive. Underwater endoscopicmucosal resection without submucosal injection (UEMR) is an innovative technique enabling en bloc resection of superficial colonlesions. We report three patients with colon adenomas extending into a diverticulum treated with successful UEMR. UEMR enabledrapid and safe en bloc resection of colon lesions extending into a diverticulum.
Hironori Yamamoto,Satoshi Shinozaki,Yoshikazu Hayashi,Yoshimasa Miura,Tsevelnorov Khurelbaatar,Hiroyuki Osawa,Alan Kawarai Lefor 대한소화기내시경학회 2019 Clinical Endoscopy Vol.52 No.2
Early detection and resection of neoplastic lesions are key objectives to diminish colorectal cancer mortality. Resection of superficialcolorectal neoplasms, cold snare polypectomy, endoscopic mucosal resection, and endoscopic submucosal dissection have all beendeveloped and used worldwide. The pocket-creation method facilitates the resection of tumors in diffcult and routine locations. Earlydetection is the most important first step to maximize the benefits of recent advancements in endoscopic techniques. However, thedetection of small, flat-shaped, or faded color lesions remains diffcult. Linked color imaging, a novel multi-light technology, facilitatesthe recognition of minor differences in tissue by enhancing the color contrast between early colorectal neoplasms and surroundingnormal mucosa in a bright field of view. The most striking feature of linked color imaging is its ability to display the color of earlyneoplastic lesions as distinct from inflammatory changes, both of which have similar “redness” when viewed using white light imaging. To increase the detection rate of neoplasms, linked color imaging should be used from the outset for endoscopic observation. Earlydetection of superficial colorectal tumors can result in decreased mortality from colorectal cancer and maintain a good quality of life forpatients.
Heng Luo,Yoshikazu Yamamoto,Hae-Sook Jeon,Yan Peng Liu,Jae Sung Jung,Young Jin Koh,허재선 한국미생물학회 2011 The journal of microbiology Vol.49 No.1
The present study was conducted to evaluate the antibacterial activity of lichen-forming fungi (LFF) against Helicobacter pylori, and to optimize the culture conditions of LFF for maximum production of natural antibiotics against H. pylori. To accomplish this, a screening assay was first conducted among 19 species of LFF. The extract of Nephromopsis pallescens (KOLRI-040516) exhibited the strongest anti-H. pylori activity. Bioautograghic TLC and HPLC analysis identified usnic acid as the main antibacterial substance produced by N. pallescens. The growth of N. pallescens and production of antibacterial substances produced by the fungus were then investigated under several culture conditions including the culture media, initial medium pHs, incubation temperatures, and the degree of aeration. The results indicated that culture in MY medium with an initial pH of 6.0, a temperature of 15°C and a low degree of aeration supported the largest usnic acid production of the fungus (16.4 μg usnic acid/g dry biomass). Especially, aeration was found to be an important factor that affect both growth and usnic acid production of N. pallescens.
The In Vitro Antioxidant Properties of Chinese Highland Lichens
( Heng Luo ),( Yoshikazu Yamamoto ),( Yan Peng Liu ),( Jae Sung Jung ),( Hyung Yeel Kahng ),( Young Jin Koh ),( Jae Seoun Hur ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.11
The antioxidant properties of 46 lichen species, collected from the highly UV-exposed alpine areas of southwestern China, were evaluated for their potential therapeutic utilization. The anti-linoleic acid peroxidation activity, 1,1- diphenyl-2-picryl-hydrazyl (DPPH) scavenging activity, reducing power, and total phenolic contents were all assessed in vitro in the methanol extract of the lichens. A potent reducing power was detected in a number of the lichen extracts, when compared with butylated hydroxyanisole (BHA). In general, it was found that many of the lichens, with antioxidant properties, contained large quantities of phenolic content. Extracts of Peltigera praetextata and Sticta nylanderiana were found to exhibit the most potent activity in all of the antioxidant tests. In particular, extracts of S. nylanderiana displayed a 1.37 times greater anti-linoleic acid peroxidation activity, when compared with the ascorbic acid used as the positive control. S. nylanderiana also possessed the strongest free radical scavenging activity amongst all the tested species, with an inhibition rate of 90.4% at concentration of 330 ?g/ml. Activity-guided bioautographic TLC and HPLC analyses were used to establish which compounds were responsible for the potent antioxidant activities of the S. nylanderiana extract. These analyses revealed lecanoric acid to be primarily responsible for the effective antioxidant properties of S. nylanderiana. Overall, these results have indicated that several highland lichens have the potential of being utilized as novel bioresources for naturally occurring antioxidant therapies.
JOSHI, Yogesh,WANG, Xin Yu,YAMAMOTO, Yoshikazu,KOH, Young Jin,HUR, Jae-Seoun Cambridge University Press 2010 The Lichenologist Vol.42 No.6
<B>Abstract</B><P>Eleven species are recognized of which <I>C. bogilana</I> and <I>C. subflavorubescens</I> are described here as new to science while nine species (<I>C. cinnabarina</I>, <I>C. decipiens</I>, <I>C. ferruginea</I>, <I>C. inconspecta</I>, <I>C. pellodella</I>, <I>C. scopularis</I>, <I>C. stantonii</I>, <I>C. squamosa</I> and <I>C. subsoluta</I>) are reported for the first time for South Korea. Both new species are peculiar due to their secondary chemistry; anthraquinones along with atranorin, gyrophoric acid and lecanoric acid in <I>C. bogilana</I>, and gyrophoric acid together with anthraquinones in <I>C. subflavorubescens</I>.</P>